Overexpression of vascular endothelial growth factor induces cell transformation in cooperation with fibroblast growth factor 2.
Oncogene
; 14(4): 463-71, 1997 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-9053843
ABSTRACT
Vascular endothelial growth factor (VEGF) is a family of homodimeric proteins produced from a single gene by alternative splicing of the VEGF transcript. VEGF induces in vivo angiogenesis and vascular permeability. We have recently demonstrated that VEGF is an autocrine growth factor for retinal pigment epithelial (RPE) cells. To further understand the role of VEGF, we overexpressed VEGF in rat RPE cells. The transfected cells exhibited a growth advantage in vitro and an increased response to the mitogenic effect of fibroblasts growth factor-2 (FGF-2), and formed colonies in soft agar upon FGF-2 addition. Moreover, analysis of FGF-receptors evidenced a dramatic increase in FGFR-1 mRNA and protein level, supporting the hypothesis that this receptor mediates the transforming effect of FGF-2. These results reveal that the oncogenic role of VEGF is exerted through a cross regulation between VEGF and FGF signal transduction pathways.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado Ocular
/
Expressão Gênica
/
Transformação Celular Neoplásica
/
Fatores de Crescimento Endotelial
/
Fator 2 de Crescimento de Fibroblastos
/
Linfocinas
/
Receptores de Fatores de Crescimento de Fibroblastos
/
Receptores Proteína Tirosina Quinases
Limite:
Animals
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França