Phosphatidylserine exposure and aminophospholipid translocase activity in Rh-deficient erythrocytes.
Mol Membr Biol
; 14(3): 125-32, 1997.
Article
em En
| MEDLINE
| ID: mdl-9394292
ABSTRACT
Endogenous phosphatidylserine (PS) exposure and lipid transport activity have been investigated for seven unrelated cases of Rhnull erythrocytes. Endogenous PS exposure was measured by prothrombinase activity. Out of six cases studied, two Rhnull samples exhibited abnormal aminophospholipid exposure, as suggested by the measurement of a lower Km of factor Xa for prothrombin. Aminophospholipid translocase activity was measured through the transbilayer redistribution of spin-labelled analogues of phospholipids. Provided that incubation conditions allow the maintainance of intracellular ATP level, no difference was observed between Rhnull and control erythrocytes, clearly indicating that the aminophospholipid translocase and Rh polypeptides are different molecular species.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilserinas
/
Sistema do Grupo Sanguíneo Rh-Hr
/
Proteínas de Transporte
/
Proteínas de Transferência de Fosfolipídeos
/
Eritrócitos Anormais
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
Mol Membr Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França