Species-specific plasmid sequences for PCR identification of the three species of Borrelia burgdorferi sensu lato involved in Lyme disease.
J Clin Microbiol
; 36(1): 269-72, 1998 Jan.
Article
em En
| MEDLINE
| ID: mdl-9431963
ABSTRACT
Species-specific sequences were shown to be carried by plasmids of the three main species of Borrelia burgdorferi sensu lato involved in Lyme disease. Libraries of the 16-, 33-, and 25-kb plasmids of B. burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii, respectively, were then built and used to isolate species-specific sequences. After sequencing of the cloned inserts, three sets of primers were designed. They were shown to determine species-specific PCR amplification products. The sensitivities of the PCR assay with these primers were 100 spirochetes for B. burgdorferi sensu stricto and 1,000 spirochetes for B. garinii and B. afzelii. The usefulness of these primers for the identification of species in biological samples (tick, serum, and cerebrospinal fluid samples) was ascertained.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Grupo Borrelia Burgdorferi
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Bélgica