Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon.
Trans R Soc Trop Med Hyg
; 91(6): 719-24, 1997.
Article
em En
| MEDLINE
| ID: mdl-9509189
ABSTRACT
Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
Palavras-chave
Africa; Africa South Of The Sahara; Age Factors; Child; Clinical Research; Clinical Trials; Demographic Factors; Developing Countries; Diseases; Drugs; Examinations And Diagnoses; French Speaking Africa; Gabon; Laboratory Examinations And Diagnoses; Laboratory Procedures; Malaria--prevention and control; Middle Africa; Parasitic Diseases; Population; Population Characteristics; Research Methodology; Research Report; Treatment; Youth
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Malária Falciparum
Limite:
Adolescent
/
Animals
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Trans R Soc Trop Med Hyg
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Reino Unido