Extrathymic T cell differentiation in vitro from human CD34+ stem cells.
J Leukoc Biol
; 64(6): 733-9, 1998 Dec.
Article
em En
| MEDLINE
| ID: mdl-9850154
ABSTRACT
Although it is well established that T cells are derived from CD34+ stem cells in vivo, and that T cells can develop in the absence of a functioning thymus, it has not proven possible thus far to generate human T cells in vitro from CD34+ cells in the absence of any thymic influence. We now present a limiting dilution cloning culture system that supports the differentiation of highly purified human CD34+ cells to CD3+ T cells in vitro in the complete absence of any thymic components. The culture system features the use of a serum-free medium supplemented with a cocktail of cytokines including flt-3 ligand, interleukin-3 (IL-3), stem cell factor (SCF), and IL-2. CD4+ T cell clones capable of mitogen-stimulated proliferation and response to IL-2, and expressing a varied TCR-Vbeta repertoire were obtained under these conditions. This culture system therefore supports human T lymphopoiesis in the absence of any thymic influence and may prove useful for the evaluation of extrathymic T cell differentiation in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Subpopulações de Linfócitos T
/
Antígenos CD34
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha