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A novel growth differentiation factor-9 (GDF-9) related factor is co-expressed with GDF-9 in mouse oocytes during folliculogenesis.
Laitinen, M; Vuojolainen, K; Jaatinen, R; Ketola, I; Aaltonen, J; Lehtonen, E; Heikinheimo, M; Ritvos, O.
Afiliação
  • Laitinen M; Department of Bacteriology and Immunology, Haartman Institute, P.O. Box 21, University of Helsinki, FIN-00014, Helsinki, Finland. mplaitin@cc.helsinki.fi
Mech Dev ; 78(1-2): 135-40, 1998 Nov.
Article em En | MEDLINE | ID: mdl-9858711
ABSTRACT
Growth differentiation factor-9 (GDF-9) is a transforming growth factor-b (TGF-b) family member which is expressed in the oocytes in mouse ovaries (McGrath, S.A., Esquela, A.F., Lee, S.J., 1995. Oocyte-specific expression of growth/differentiation factor-9. Mol. Endocrinol. 9, 131-136). GDF-9 is indispensable for normal folliculogenesis since female mice deficient for the GDF-9 gene are infertile due to an arrest of follicular growth at the primary follicle stage (Dong, J., Albertini, D.F., Nishimori, K., Kumar, T.R. , Lu, N., Matzuk, M.M., 1996. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 383, 531-535). We searched the GenBank Expressed Sequence Tag (EST) database with the mouse GDF-9 cDNA sequence, and identified from a mouse 2-cell embryo library an EST cDNA that encodes a putative member of the TGF-b superfamily, and named it as GDF-9B. Northern blot hybridization analyses of mouse ovaries revealed a single transcript of approximately 4.0 kilobases (kb) for GDF-9B and of 2.0 kb for GDF-9. We cloned by reverse transcription-polymerase chain reaction from mouse ovarian RNA a partial 821-base pair GDF-9B cDNA that spans the sequence encoding the putative mature region of GDF-9B. The COOH-terminal region of GDF-9B appears to be 53% homologous to GDF-9. Moreover, like GDF-9, GDF-9B lacks the cysteine residue needed for the covalent dimerization of several TGF-b family members. Using in situ hybridization analysis, we demonstrate that GDF-9B and GDF-9 mRNAs are co-localized in the oocyte. We also show that GDF-9B and GDF-9 genes are co-ordinately expressed during follicular development.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Oogênese / Proteínas do Ovo / Diferenciação Celular / Substâncias de Crescimento / Regulação da Expressão Gênica no Desenvolvimento / Isoformas de Proteínas / Peptídeos e Proteínas de Sinalização Intercelular / Folículo Ovariano Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mech Dev Assunto da revista: EMBRIOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Finlândia
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Oogênese / Proteínas do Ovo / Diferenciação Celular / Substâncias de Crescimento / Regulação da Expressão Gênica no Desenvolvimento / Isoformas de Proteínas / Peptídeos e Proteínas de Sinalização Intercelular / Folículo Ovariano Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mech Dev Assunto da revista: EMBRIOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Finlândia