RESUMO
P-selectin glycoprotein ligand 1 (PSGL-1) is a sialomucin expressed on leukocytes that mediates neutrophil rolling on the vascular endothelium. Here, the role of PSGL-1 in mediating lymphocyte migration was studied using mice lacking PSGL-1. In a contact hypersensitivity model, the infiltration of CD4(+) T lymphocytes into the inflamed skin was reduced in PSGL-1-deficient mice. In vitro-generated T helper (Th)1 cells from PSGL-1-deficient mice did not bind to P-selectin and migrated less efficiently into the inflamed skin than wild-type Th1 cells. To assess the role of PSGL-1 in P- or E-selectin-mediated migration of Th1 cells, the cells were injected into E- or P-selectin-deficient mice. PSGL-1-deficient Th1 cells did not migrate into the inflamed skin of E-selectin-deficient mice, indicating that PSGL-1 on Th1 cells is the sole ligand for P-selectin in vivo. In contrast, PSGL-1-deficient Th1 cells migrated into the inflamed skin of P-selectin-deficient mice, although less efficiently than wild-type Th1 cells. This E-selectin-mediated migration of PSGL-1-deficient or wild-type Th1 cells was not altered by injecting a blocking antibody to L-selectin. These data provide evidence that PSGL-1 on Th1 cells functions as one of the E-selectin ligands in vivo.
Assuntos
Quimiotaxia de Leucócito , Dermatite Alérgica de Contato/imunologia , Selectina E/imunologia , Glicoproteínas de Membrana/imunologia , Selectina-P/imunologia , Células Th1/imunologia , Adjuvantes Imunológicos , Animais , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/fisiologia , Células CHO , Cricetinae , Dermatite Alérgica de Contato/patologia , Modelos Animais de Doenças , Selectina E/genética , Selectina E/metabolismo , Ligantes , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Oxazolona/efeitos adversos , Selectina-P/genética , Selectina-P/metabolismo , Pele/imunologia , Pele/patologia , Células Th1/metabolismo , Células Th1/fisiologiaRESUMO
Sub-optimal accrual rates to adult oncology clinical trials have been reported. One potential accrual barrier has been the step of identifying potential clinical trials for patients. In order to partially address this barrier, a clinical trial listing was developed for oncologists to use on their personal digital assistants (PDAs). Preliminary feedback was gathered from an initial group of physicians who accessed this resource. Benefits of this approach as well as future enhancements are discussed.