RESUMO
A strain of camel kidney cells was developed and carried in serial passages. The subcultures were slow-growing in the early passages and were composed of heterogeneous cell population. By the 35th passage, the growth rate increased, and more homogeneous cells, mostly of the epithelioid type, were seen. The cell strain was highly susceptible to West Nile, Sindbis, vesicular stomatitis, adeno, and vaccinia viruses, and also was susceptible to herpes simplex, rinderpest, measles, and canine distemper viruses.
Assuntos
Camelus , Técnicas de Cultura , Rim , Vírus/patogenicidade , Animais , Linhagem Celular , Efeito Citopatogênico Viral , Métodos , Vírus/isolamento & purificaçãoRESUMO
The results obtained with Brucella B19 strain, used as adjuvant and stimulant in the preparation of hyperimmune anti-foot-and-mouth disease serums on guinea pigs, have led the authors to carry out a series of experiments with a combined anti-foot-and-mouth disease and anti-brucellosis vaccine (strain B 19 Buck and Cotton). The search for neutralizing antibodies for foot-and-mouth disease has been undertaken by different methods. In the search for antibrucellic antibodies reactions such as seroagglutination, fixation of the complement, etc. have been used. The results obtained with combined vaccine are identical with those obtained with each of the monovalent vaccines.