Bark regeneration and tannin content in Myracrodruon urundeuva Allemão after simulation of extractive damages--implications to management.

Due to the current exploitation and consequent extinction of native medicinal plants around the world, new strategies have been proposed to address the sustainable use of this resource. Accordingly, the goal of this study is to assess the speed of bark regeneration of Myracrodruon urundeuva Allemão and to compare the tannin content before and after tissue regeneration. Twenty individuals from an area of Caatinga in Caruaru, Pernambuco State (NE Brazil), were selected. To evaluate the speed of bark regeneration, four treatments were established, according to the area of bark removed (10 × 2, 6 × 5, 8 × 5 and 10 × 5 cm), with five replicates (five individuals of M. urundeuva Allemão) and three treatments of tannin content (bark removed before damage, as regenerated tissue, and non-impacted bark or control). At the end of 23 months of monitoring the regeneration, we found that only seven of the 20 individuals analyzed did not fully heal. No significant correlation between the monthly percent regeneration and average monthly precipitation was found. The tannin content varied according to the quantified tissue (after damage, regenerated and control) as well as between damage classes (20 cm(2)-41.64 to 63.53 mg; 30 cm(2)-49.25 to 67.54 mg; 40 cm(2)-31.69 to 67.44 mg; 50 cm(2)-34.08 to 48.53 mg). Despite the variations found, there was no significant difference between the measurement periods (p > 0.05) or damage classes (p > 0.05). The results showed that the regeneration rate was higher in individuals belonging to the 10 × 2 and 10 × 5 cm groups and that there is no correlation between precipitation and regeneration speed. Tannin levels did not vary significantly in the tissues before damage and after regeneration.

Cytotoxic pyranonaphthoquinones from Melloa quadrival vis (Bignoniaceae).

Three new pyranonaphthoquinones: 5-hydroxy-6-methoxy-alpha-lapachone, 5,6-dihydroxy-a-lapachone and 4',5-dihydroxy-6-methoxy-alpha-lapachone, and two known compounds: lapachol and 5,5'-dihydroxy-3',4',7-trimethoxyflavanone, were isolated from the stem bark of Melloa quadrivalvis. Their structures were established by spectrometric data, mainly 1D- and 2D-NMR and mass spectra. The methylazoetetrazolium (MTT) method using viable cells of the strain Hep2 and the strain NCIH-292 demonstrated cytotoxic activity. The CI50 was also calculated. The chloroform extract and 5-hydroxy-6-methoxy-alpha-lapachone inhibited cell growth.

Evaluation of antihyperglycaemic activity of Calotropis procera leaves extract on streptozotocin-induced diabetes in Wistar rats

Calotropis procera (Aiton) W.T.Aiton,Apocynaceae, popularly known as "algodão-de-seda", is a wild African bush, rich in bioactive substances that determine the medicinal potential of this species. Diabetes mellitus is a disease that affects about 10% of the population. This study aimed to evaluate the antihyperglycaemic activity of the hydroalcoholic extract of the leaves of C. procera of occurrence in coast of Pernambuco, Brazil. The hydroalcholic extract of the leaves of C. procera (300 and 600 mg/kg/day), vehicle, insulin (6U, s.c.) or metformin (500 mg/ kg/day) were administered orally to streptozotocin-induced diabetic rats (n = 7/group) for four weeks. Changes in body weight, food and water intake, biochemical markers, fasting glucose levels and oral glucose tolerance test were evaluated. The results showed that the C. procera dried extract (300 and 600 mg/kg) reduced significantly the level of blood glucose throughout the evaluation period and improved metabolic status of the animals and ameliorate the oral tolerance glucose test. The phytochemical screening revealed and quantified the presence of phenolic compounds and flavonoids in a percentage of 29.1 and 2.9%, respectively. Thus, we conclude that the extract of the leaves of C. procera has antihyperglycemic activity.

Análise da pluviosidade e do efeito de borda sobre os teores de flavonóides em Bauhinia cheilantha (Bong.) Steud., Fabaceae / Analysis of rainfall and edge-effects on flavonoid levels in Bauhinia cheilantha (Bong.) Steud., Fabaceae

Este estudo avaliou a influência do efeito de borda e da pluviosidade sobre a produção de flavonóides em indivíduos de Bauhinia cheilantha (Bong.) Steud., Fabaceae, em uma área de caatinga no estado de Pernambuco, Nordeste do Brasil. Foi utilizada metodologia analítica por complexação com cloreto de alumínio para quantificar a concentração de flavonóides contidos nos extratos foliares de B. cheilantha, por meio de espectrofotometria no visível. De forma geral, o efeito de borda afeta a produção de flavonóides, entretanto, não foi possível correlacionar a produção de flavonóides e a pluviosidade, demonstrando que a espécie avaliada utiliza outra estratégia como resposta às pressões ambientais.

The present study evaluated the influence of the edge-effect and rainfall on flavonoid content in individuals of Bauhinia cheilantha (Bong.) Steud., Fabaceae, in an area of caatinga vegetation in Pernambuco State, northeastern Brazil. The analytical methodology used aluminum chloride binding to quantify flavonoid concentrations by visible light spectrophotometry in leaf extracts of B. cheilantha. In general, forest edges influenced flavonoid production, but it was not possible to relate production with rainfall levels. These results demonstrate that this species uses various strategies to respond to environmental variables.

Influence of biflorin on the labelling of red blood cells, plasma protein, cell protein, and lymphocytes with technetium-99m: in vitro study

In this paper we report the results of an in vitro study involving the influence of biflorin (an o-quinone isolated from Capraria biflora L. that has potent antimicrobial activity) on the Tc-99m labeling of red blood cells, plasma protein, cells protein, and lymphocytes. Blood was withdrawn from Wistar rats and incubated with various concentrations of biflorin, and solutions of stannous chloride and Tc-99m were added. Plasma (P) and red blood cells (RBC) were isolated, precipitated, and centrifuged, and soluble (SF) and insoluble (IF) fractions were isolated. The results show that the highest concentration (100 percent) of biflorin is able to reduce the uptake of Tc-99m ( percentATI) on RBC and the fixation on IF-P. To study the influence of biflorin on 99mTc lymphocyte labeling, human blood was submitted to a technique with Ficoll-Hypac and centrifuged, and white cells were isolated. Lymphocytes (2.5 mL; 1.0 x 10(6) cells/mL) were obtained and a 0.2 mL solution was incubated with biflorin (0.1 mL). Solutions of stannous chloride and 99mTc were added. Lymphocytes were separated and the percentATI bound in these cells was evaluated. A reduction in percentATI (from 97.85 ± 0.99 to 88.86 ± 5) was observed for RBC and for IF-P (73.24 ± 5.51 to 20.72 ± 6.95). In this case the results showed no decrease in percentATI for the lymphocytes with biflorin.

Neste artigo relatam-se os resultados de um estudo in vitro envolvendo a influência da biflorina (uma o-quinona isolada de Capraria biflora L. que possui uma potente atividade antimicrobiana) na marcação do Tc-99m em células vermelhas do sangue, proteínas do plasma, proteínas celulares e em linfócitos. O sangue foi coletado de ratos Wistar e incubado com várias concentrações de biflorina, e soluções de cloreto estanoso (SnCl2) adicionando-se Tc-99m. O plasma (P) e as células vermelhas do sangue (CVS) foram isolados, precipitados e centrifugados, isolando-se as frações solúveis (FS) e insolúveis (FI). A maior concentração de biflorina (100 por cento) é capaz de reduzir a captação do Tc-99m ( por centoATI) nas CVS e a fixação na FI-P. Uma solução de 0,2 mL de linfócitos (2,5 mL; 1.0 x 10(6) células/mL), obtidos por centrifugação de sangue humano tratado com Ficoll-Hypac, foi incubada com biflorina (0,1 mL). Soluções de cloreto estanoso e Tc-99m foram então adicionadas. Os linfócitos foram separados e o por centoATI presente nessas células foi avaliado. Uma redução no por centoATI (de 97,85 ± 0,99 a 88,86 ± 5) foi observada para CVS e para FI-P (73,24 ± 5,51 a 20,72 ± 6,95). Os resultados não mostraram decréscimo no por centoATI para os linfócitos com biflorina.

The effects of seasonal climate changes in the caatinga on tannin levels in Myracrodruon urundeuva (Engl.) Fr. All. and Anadenanthera colubrina (Vell.) Brenan / Os efeitos da sazonalidade climática na caatinga sobre os teores de taninos em Myracrodruon urundeuva (Engl.) Fr. All. e Anadenanthera colubrina (Vell.) Brenan

The influence of seasonal climatic changes on tannin concentrations was examined in two caatinga (semi-arid) plant species (Myracrodruon urundeuva (Engl.) Fr. All. and Anadenanthera colubrina (Vell.) Brenan). The Folin-Ciocalteau method was used to quantify total phenols, and the casein precipitation method was used for tannins. In general, there is a close relationship between tannin levels and rainfall, although species seem to adopt different strategies of tannin compound production in response to periods of drought and rainfall in the caatinga.

Examinou-se a influência da sazonalidade climática sobre as concentrações de taninos em duas espécies da Caatinga: Myracrodruon urundeuva (Engl.) Fr. All. e Anadenanthera colubrina (Vell.) Brenan. Empregou-se o método Folin-Ciocalteau para quantificar os fenóis totais e o método de precipitação da caseína para taninos. De maneira geral, existe uma forte relação entre os teores de taninos e a pluviosidade, apesar das espécies parecerem adotar diferentes estratégias de produção de compostos tânicos em resposta aos períodos de seca e chuva na caatinga.