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1.
J Dairy Sci ; 107(5): 2883-2899, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38101733

RESUMO

Net energy for lactation (NEL) and metabolizable protein (MP) are the 2 main nutritional forces that drive synthesis of milk components. This study investigated mammary-gland metabolism in dairy cows in response to variations in the supply of NEL and MP. Four Holstein dairy cows were randomly assigned to a 4 × 4 Latin square design, in which each experimental period consisted of 14 d of dietary treatment. The diets provided 2 levels of NEL (low energy, 25.0 Mcal/d vs. high energy, 32.5 Mcal/d) and 2 levels of MP (low protein, 1,266 g/d vs. high protein, 2,254 g/d of protein digestible in the intestine) in a 2 × 2 factorial arrangement. Performance and dry matter intake (DMI) were measured during the last 5 d of each period, and the mammary net balance was measured on d 13 by collecting 6 sets of blood samples from the left carotid artery and left mammary vein. Mammary plasma flow was measured according to the Fick principle for Phe and Tyr. The mammary net balance of carbon equaled the uptake of nutrients expressed as carbon minus the output of lactose, fatty acids (FA) synthesized in the mammary gland, AA of milk protein, and glycerol-3P from triglyceride on d 13. Milk, lactose, fat, and protein yields increased when NEL and MP supplies increased. However, increasing the NEL supply increased FA synthesis more than increasing the protein supply did. In addition, FA secretion increased more than lactose secretion when the NEL supply increased. Increasing the NEL supply increased the left half-udder uptake of all major energy-yielding nutrients by increasing mammary plasma flow. However, nutrient uptake increased more than milk output did, which in turn increased carbon dioxide output. This increase in nutrient oxidation by the mammary gland decreased the mammary efficiency of nutrients utilization when the NEL supply increased. Increasing MP supply tended to increase glucose uptake through mammary clearance and increased mammary AA uptake with no change in mammary plasma flow. In addition, the protein supply did not change the mammary uptake of acetate or ß-hydroxybutyrate. The increase in milk-component secretions in response to either NEL or MP supplies occurred through different metabolic adaptations (increase in mammary plasma flow vs. clearances, respectively). These results suggest that the nutrient use by the mammary gland is highly flexible, which helps in maintaining milk and milk-component yields even with limiting nutrient supplies.


Assuntos
Lactose , Leite , Feminino , Bovinos , Animais , Lactose/metabolismo , Leite/metabolismo , Lactação/fisiologia , Proteínas do Leite/metabolismo , Dieta/veterinária , Ácidos Graxos/metabolismo , Metabolismo Energético/fisiologia
4.
Hernia ; 21(4): 637-642, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28233069

RESUMO

PURPOSE: Synthetic mesh for herniorrhaphy has been placed under critical observation regarding the potential association of mesh placement and the subsequent development of autoimmune diseases. We sought to evaluate whether there is a link between synthetic polypropylene mesh repairs and the subsequent development of systemic/autoimmune disorders (SAID). STUDY DESIGN: Adult men undergoing hernia repair with mesh between January 2008 and December 2009 in New York State were identified using International Classification of Diseases, Ninth Revision, Modification procedure codes and Current Procedural Terminology Coding System, Fourth Edition codes. A control cohort of men undergoing colonoscopy was created with whom to compare outcomes. RESULTS: A total of 29,712 patients underwent hernia repair between January 2008 and December 2009. In the control cohort, 79,265 patients underwent colonoscopy. During the entire follow-up, 475 patients undergoing hernia repair and 1305 patients in the control cohort were diagnosed with autoimmune disease. When patients were matched based on demographics, comorbidities and procedure date, hernia repair was not associated with an increased risk of developing autoimmune disease over the entire follow-up time period. 1.6% of those in the hernia group vs. 1.7% of those in the colonoscopy group developed SAID [risk ratio (95% CI): hernia vs. colonoscopy 0.93(0.79-1.09)]. No association between mesh surgery and increased risks of SAID was found at any of the specified time points (6 months, 1 year, and 2-year follow-up). CONCLUSIONS: Mesh-based hernia repair was not associated with the development of autoimmune diseases compared to those undergoing routine screening colonoscopy.


Assuntos
Doenças Autoimunes/etiologia , Herniorrafia/instrumentação , Polipropilenos/imunologia , Telas Cirúrgicas/efeitos adversos , Adulto , Idoso , Estudos de Coortes , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva , Estudos Retrospectivos , Risco
5.
FEBS Lett ; 443(3): 321-5, 1999 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-10025956

RESUMO

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Antimônio/farmacologia , Resistência a Múltiplos Medicamentos , Metais Pesados/farmacologia , Regulação para Cima/efeitos dos fármacos , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores , Transportadores de Cassetes de Ligação de ATP/metabolismo , Antimônio/metabolismo , Antineoplásicos/farmacologia , Arsenitos/farmacologia , Western Blotting , Cloreto de Cádmio/farmacologia , Resistencia a Medicamentos Antineoplásicos , Fluoresceínas/metabolismo , Amplificação de Genes/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Neoplasias Pulmonares , Meglumina/farmacologia , Metalotioneína/metabolismo , Proteínas Associadas à Resistência a Múltiplos Medicamentos , Propionatos/farmacologia , Quinolinas/farmacologia , RNA Mensageiro/metabolismo , Células Tumorais Cultivadas , Sulfato de Zinco/farmacologia
6.
Biochem Pharmacol ; 61(11): 1387-91, 2001 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-11331074

RESUMO

The human multidrug-resistance protein (MRP1) confers resistance to some heavy metals such as arsenic and antimony, mainly through mediating an increased cellular efflux of metal. However, it was recently suggested that arsenic, used under its trioxide derivative form as anticancer drug, is not handled by MRP1. The aim of the present study was to test this hypothesis in MRP1-overexpressing human lung tumor GLC4/Sb30 cells. Using the cytotoxicity MTT assay, GLC4/Sb30 cells were found to be 10.8-fold more resistant to arsenic trioxide (As2O3) than parental GLC4 cells. MK571, a potent inhibitor of MRP1 activity, almost totally reversed resistance of GLC4/Sb30 cells, but did not alter the sensitivity of GLC4 cells. Moreover, As2O3-loaded GLC4/Sb30 cells poorly accumulated arsenic through an increased MK571-sensitive efflux of metal. Finally, depletion of cellular glutathione levels in buthionine sulfoximine-treated GLC4/Sb30 cells was found to result in increased accumulation and reduced efflux of arsenic in cells exposed to As2O3, outlining the glutathione-dependence of MRP1-mediated transport of the metal. These results indicate that MRP1 overexpression in human tumor cells can confer resistance to As2O3, which may limit the clinical use of this anticancer drug for treatment of MRP1-positive tumors.


Assuntos
Transportadores de Cassetes de Ligação de ATP/biossíntese , Antineoplásicos/farmacologia , Arsenicais/farmacologia , Óxidos/farmacologia , Transportadores de Cassetes de Ligação de ATP/fisiologia , Trióxido de Arsênio , Sobrevivência Celular/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/fisiologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Proteínas Associadas à Resistência a Múltiplos Medicamentos , Células Tumorais Cultivadas
7.
Eur J Pharmacol ; 330(2-3): 231-40, 1997 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-9253958

RESUMO

The effects of arachidonic acid ethanolamide (anandamide), palmitoylethanolamide and delta9-tetrahydrocannabinol on the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-4, interleukin-6, interleukin-8, interleukin-10, interferon-gamma, p55 and p75 TNF-alpha soluble receptors by stimulated human peripheral blood mononuclear cells as well as [3H]arachidonic acid release by non-stimulated and N-formyl-Met-Leu-Phe (fMLP)-stimulated human monocytes were investigated. Anandamide was shown to diminish interleukin-6 and interleukin-8 production at low nanomolar concentrations (3-30 nM) but inhibited the production of TNF-alpha, interferon-gamma, interleukin-4 and p75 TNF-alpha soluble receptors at higher concentrations (0.3-3 microM). Palmitoylethanolamide inhibited interleukin-4, interleukin-6, interleukin-8 synthesis and the production of p75 TNF-alpha soluble receptors at concentrations similar to those of anandamide but failed to influence TNF-alpha and interferon-gamma production. The effect of both compounds on interleukin-6 and interleukin-8 production disappeared with an increase in the concentration used. Neither anandamide nor palmitoylethanolamide influenced interleukin-10 synthesis. delta9-Tetrahydrocannabinol exerted a biphasic action on pro-inflammatory cytokine production. TNF-alpha, interleukin-6 and interleukin-8 synthesis was maximally inhibited by 3 nM delta9-tetrahydrocannabinol but stimulated by 3 microM delta9-tetrahydrocannabinol, as was interleukin-8 and interferon-gamma synthesis. The level of interleukin-4, interleukin-10 and p75 TNF-alpha soluble receptors was diminished by 3 microM delta9-tetrahydrocannabinol. [3H]Arachidonate release was stimulated only by high delta9-tetrahydrocannabinol and anandamide concentrations (30 microM). These results suggest that the inhibitory properties of anandamide, palmitoylethanolamide and delta9-tetrahydrocannabinol are determined by the activation of the peripheral-type cannabinoid receptors, and that various endogenous fatty acid ethanolamides may participate in the regulation of the immune response.


Assuntos
Ácido Araquidônico/metabolismo , Ácidos Araquidônicos/farmacologia , Citocinas/metabolismo , Dronabinol/farmacologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Ácidos Palmíticos/farmacologia , Amidas , Ácido Araquidônico/sangue , Citocinas/sangue , Endocanabinoides , Etanolaminas , Humanos , Alcamidas Poli-Insaturadas , Taxa Secretória/efeitos dos fármacos
8.
Toxicology ; 142(2): 127-34, 2000 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-10685512

RESUMO

The human multidrug-resistance protein (MRP1), known to mediate cellular efflux of a wide range of xenobiotics, including anticancer drugs, has also been shown to transport antimony, thereby conferring resistance to this heavy metal. The aim of the present study was to investigate whether other cytotoxic metals could be handled by MRPI using MRP1-overexpressing lung tumor GLC4/Sb30 cells. Such cells were found to be 3.4-, 12.7- and 16.3-fold more resistant than parental GLC4 cells to mercuric ion, arsenite and arsenate, respectively, whereas they remained sensitive to other cytotoxic metals tested such as copper, chromium, cobalt or aluminium. MK571, a potent inhibitor of MRP1 activity, almost totally reversed resistance of GLC4/Sb30 cells to mercuric ions and arsenic while it did not significantly alter sensitivity of GLC4 cells to metals. Arsenate-treated GLC4/Sb30 cells were found to poorly accumulate arsenic through increased MK571-inhibitable efflux of the metal. Arsenate, however, failed to alter MRP1-mediated transport of known MRP1 substrates such as calcein and vincristine. In conclusion, these findings demonstrated that MRP1 likely handled some, but not all, cytotoxic metals such as arsenic and mercuric ions in addition to antimony, therefore resulting in reduced toxicity of these compounds towards MRP1-overexpressing cells.


Assuntos
Transportadores de Cassetes de Ligação de ATP/fisiologia , Metais/toxicidade , Transportadores de Cassetes de Ligação de ATP/análise , Antimônio/metabolismo , Antimônio/toxicidade , Arsênio/metabolismo , Arsênio/toxicidade , Resistência a Múltiplos Medicamentos , Humanos , Neoplasias Pulmonares/patologia , Mercúrio/metabolismo , Mercúrio/toxicidade , Proteínas Associadas à Resistência a Múltiplos Medicamentos , Células Tumorais Cultivadas
9.
Life Sci ; 59(22): 1829-37, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8950280

RESUMO

Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in NIH-3T3 fibroblasts. The furosemide-sensitive Na+/K+/Cl- cotransport and the ouabain-sensitive Na+/K(+)-ATPase were both involved in Rb+ influx under resting conditions with a ratio Na+/K+/Cl- cotransport/Na+/K(+)-ATPase (r) = 0.73. Bradykinin stimulated Rb+ influx (+82.6%) through both systems without changing their ratio (r = 0.72). PKC stimulation by a 15-min-treatment with phorbol 12-myristate 13-acetate (PMA) (2x10(-7) M) increased Rb+ influx in resting cells by 75.7% without affecting r (0.75). PKC inhibition by H-7, and PKC down-regulation by 24-h PMA (10(-6) M) treatment decreased the bradykinin-induced stimulation of Rb+ influx (+31% and +14.9% above control, respectively). Both down-regulation and inhibition of PKC dramatically reduced the furosemide-sensitive Na+/K+/Cl- cotransport, as r fell to 0.239 and 0.032 in bradykinin-stimulated cells after H-7 and 24-h PMA treatments, respectively. BAPTA/AM pretreatment (10(-4) M, 60 min), which complexed with [Ca2+]i, not only prevented the bradykinin-induced [Ca2+]i raise, but also partially inhibited bradykinin-induced Rb+ influx stimulation (+39% above control), without modifying r (0.76). We conclude that stimulation of PKC is a major pathway involved in bradykinin stimulation of Rb+ influx in NIH-3T3 fibroblasts, and that rises in [Ca2+]i participate in bradykinin signalling, possibly through PKC activation. Our data also suggest that active PKC is required for basal and bradykinin-stimulated Na+/K+/Cl- cotransport activity in these cells.


Assuntos
Bradicinina/farmacologia , Proteínas de Transporte/efeitos dos fármacos , Rubídio/farmacologia , ATPase Trocadora de Sódio-Potássio/efeitos dos fármacos , Células 3T3 , Animais , Proteínas de Transporte/metabolismo , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Furosemida/farmacologia , Transporte de Íons , Camundongos , Proteína Quinase C/metabolismo , Simportadores de Cloreto de Sódio-Potássio , ATPase Trocadora de Sódio-Potássio/metabolismo
10.
Rofo ; 133(2): 158-63, 1980 Aug.
Artigo em Alemão | MEDLINE | ID: mdl-6449432

RESUMO

Partial and total trans-catheter embolisation of the spleen with butyl-2-cyanoacrylate-lipiodol in 23 dogs lead to partial necrosis and cicatrisation of the spleen after 3 to 5 weeks. The embolised vessels do not recanalise, but are by-passed by extra- and intra-splenic collaterals. In five animals there was a reduction in the number of erythrocytes; after embolisation there was increase in the number of leucocytes, whereas the thrombocytes were unchanged. The most common complication which occurred in five animals was a 1.5 to 2.5 cm. splenic abscess. The procedure was employed without success in one patient with bleeding oesophageal varices. The procedure was complicated by extensive necrosis of the gastric mucosa. Trans-catheter embolisation of the spleen has significant complications, despite strict asepsis and antibiotic cover. The main indication of the procedure is hypersplenism which cannot be treated surgically.


Assuntos
Embolização Terapêutica/normas , Hiperesplenismo/terapia , Artéria Esplênica , Animais , Bucrilato , Circulação Colateral , Cães , Embolização Terapêutica/efeitos adversos , Varizes Esofágicas e Gástricas/terapia , Mucosa Gástrica , Humanos , Masculino , Pessoa de Meia-Idade , Necrose/etiologia , Peritonite/etiologia
11.
J Anal Toxicol ; 24(2): 90-2, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10732945

RESUMO

A 39-year-old man committed suicide by ingestion of aluminum phosphide, a potent mole pesticide, which was available at the victim's workplace. The judicial authority ordered an autopsy, which ruled out any other cause of death. The victim was discovered 10 days after the ingestion of the pesticide. When aluminum phosphide comes into contact with humidity, it releases large quantities of hydrogen phosphine (PH3), a very toxic gas. Macroscopic examination during the autopsy revealed a very important asphyxia syndrome with major visceral congestion. Blood, urine, liver, kidney, adrenal, and heart samples were analyzed. Phosphine gas was absent in the blood and urine but present in the brain (94 mL/g), the liver (24 mL/g), and the kidneys (41 mL/g). High levels of phosphorus were found in the blood (76.3 mg/L) and liver (8.22 mg/g). Aluminum concentrations were very high in the blood (1.54 mg/L), brain (36 microg/g), and liver (75 microg/g) compared to the usual published values. Microscopic examination revealed congestion of all the organs studied and obvious asphyxia lesions in the pulmonary parenchyma. All these results confirmed a diagnosis of poisoning by aluminum phosphide. This report points out that this type of poisoning is rare and that hydrogen phosphine is very toxic. The phosphorus and aluminum concentrations observed and their distribution in the different viscera are discussed in relation to data in the literature.


Assuntos
Compostos de Alumínio/intoxicação , Praguicidas/intoxicação , Fosfinas/intoxicação , Adulto , Alumínio/análise , Compostos de Alumínio/análise , Química Encefálica , Humanos , Rim/química , Fígado/química , Masculino , Praguicidas/análise , Fosfinas/análise , Fósforo/análise , Suicídio
12.
Environ Toxicol Chem ; 20(11): 2530-6, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11699779

RESUMO

Selectivity is an important factor in identifying candidate pesticides to be used in crop protection since it characterizes chemicals that, while being effective against target pests, exert an acceptable impact on the other components of the environment. Extrapolated to an integrated pest management (IPM) context, selectivity implies that candidate pesticides may preserve the ability of beneficial insects to significantly control target pest populations. In the present study, we assess the physiological selectivity of the organophosphate chlorfenvinphos, used to protect cruciferous crops against the cabbage root fly, Delia radicum (Diptera: Anthomyiidae), by investigating both the lethal and sublethal effects exerted on its main parasitoid Trybliographa rapae (Hymenoptera: Figitidae). The comparison of the median lethal doses showed that T. rapae was at least seven times less sensitive than D. radicum to chlorfenvinphos. However, longevity of parasitoids surviving a sublethal dose was reduced by half. The potential fecundity of females was decreased by 9.6 to 22.8%. Chlorfenvinphos also induced important behavioral changes in both sexes and reduced the chances for parasitoids to mate by more than 70%. While most behavioral changes were reversible, effects on mating and on fecundity were not, thereby suggesting long-term effects on the reproduction of the parasitoid. These cumulative effects of chlorfenvinphos would have dramatic consequences on the efficacy of parasitoids contacting such doses of chlorfenvinphos in the field and therefore there is question about the intrinsic selectivity of this insecticide.


Assuntos
Clorfenvinfos/toxicidade , Dípteros , Himenópteros , Inseticidas/toxicidade , Animais , Comportamento Animal/efeitos dos fármacos , Feminino , Fertilidade/efeitos dos fármacos , Controle de Insetos , Dose Letal Mediana , Masculino , Parasitos , Medição de Risco
13.
Sao Paulo Med J ; 116(5): 1826-8, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-10030110

RESUMO

The authors report the case of an infant with an extensive face hemangioma with subglottic airway obstruction which had been successfully treated with interferon alpha 2A but then reoccurred with the same dimensions and airway blockage after treatment was abruptly interrupted. The authors suggest the implementation of a standard procedure for the interruption of interferon alpha 2A treatment in order to avoid this rebound effect and advise on the need for further studies to properly evaluate dosage and administration parameters for interferon alpha 2A in the treatment of difficult hemangioma.


Assuntos
Obstrução das Vias Respiratórias/etiologia , Antineoplásicos/uso terapêutico , Neoplasias Faciais/tratamento farmacológico , Hemangioma/tratamento farmacológico , Interferon-alfa/uso terapêutico , Obstrução das Vias Respiratórias/cirurgia , Neoplasias Faciais/complicações , Feminino , Hemangioma/complicações , Humanos , Recém-Nascido , Interferon alfa-2 , Proteínas Recombinantes , Traqueostomia
14.
J Pharm Belg ; 49(5): 395-401, 1994.
Artigo em Francês | MEDLINE | ID: mdl-7837032

RESUMO

The debated consumption of germanium suggested the authors to compare biopharmaceutical parameters of germanium oxide and germanium sesquioxide. A first evaluation, in rabbit, has been based on Germanium blood levels determined by atomic absorption spectrometry, after cross administration of both products by the I.V. and oral routes. When given orally, the apparent oxide bioavailability is very low (about 10%) but better than that of the sesquioxide. That difference could result from differences of disposition parameters of both products, which have to be studied late.


Assuntos
Antimutagênicos/farmacocinética , Germânio/farmacocinética , Animais , Disponibilidade Biológica , Masculino , Coelhos
15.
Contemp Clin Trials ; 33(5): 1011-8, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22643040

RESUMO

INTRODUCTION: Robotic assistance during laparoscopic surgery for pelvic organ prolapse rapidly disseminated across the United States without level I data to support its benefit over traditional open and laparoscopic approaches [1]. This manuscript describes design and methodology of the Abdominal Colpopexy: Comparison of Endoscopic Surgical Strategies (ACCESS) Trial. METHODS: ACCESS is a randomized comparative effectiveness trial enrolling patients at two academic teaching facilities, UCLA (Los Angeles, CA) and Loyola University (Chicago, IL). The primary aim is to compare costs of robotic assisted versus pure laparoscopic abdominal sacrocolpopexy (RASC vs LASC). Following a clinical decision for minimally-invasive abdominal sacrocolpopexy (ASC) and research consent, participants with symptomatic stage≥II pelvic organ prolapse are randomized to LASC or RASC on the day of surgery. Costs of care are based on each patient's billing record and equipment costs at each hospital. All costs associated with surgical procedure including costs for robot and initial hospitalization and any re-hospitalization in the first 6weeks are compared between groups. Secondary outcomes include post-operative pain, anatomic outcomes, symptom severity and quality of life, and adverse events. Power calculation determined that 32 women in each arm would provide 95% power to detect a $2500 difference in total charges, using a two-sided two sample t-test with a significance level of 0.05. RESULTS: Enrollment was completed in May 2011. The 12-month follow-up was completed in May 2012. CONCLUSIONS: This is a multi-center study to assess cost as a primary outcome in a comparative effectiveness trial of LASC versus RASC.


Assuntos
Colposcopia/métodos , Pesquisa Comparativa da Efetividade/métodos , Laparoscopia/métodos , Robótica/métodos , Prolapso Uterino/cirurgia , Colposcopia/economia , Análise Custo-Benefício , Feminino , Humanos , Laparoscopia/economia , Estudos Multicêntricos como Assunto/métodos , Complicações Pós-Operatórias , Anos de Vida Ajustados por Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto/métodos , Robótica/economia , Método Simples-Cego
19.
Acta Clin Belg ; 61 Suppl 1: 25-31, 2006.
Artigo em Francês | MEDLINE | ID: mdl-16700147

RESUMO

The famous Lafarge affair which took place in Tulle (Corrèze, France) in 1840, has never ceased to interest the general public because some doubts have remained even after the pronunciation of the judgment. Another hypothesis than arsenicum poisoning has been formulated in 1980 by Professor P. Lepine who believes in a paratyphoid fever occuring after food poisoning. This assumption and the discovery of exceptional family documents in 2004 by Edouard de Lamaze, a distant cousin of Emma Pontier, the confidante of Marie Lafarge, sheds a new light on this mysterious affair.


Assuntos
Intoxicação por Arsênico/história , Homicídio/história , França , História do Século XIX , Toxicologia/normas
20.
Rev Paul Med ; 111(2): 363-6, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8284580

RESUMO

In this preliminary note the authors describe a new technique consisting in the use of videoscopy as an aid in surgery for the placement of tissue expanders in a patient with sequelae due to burn scarring on the posterior thorax. The surgical steps are described, together with the technical details of the use of the videolaparoscopy equipment. The authors discuss the advantages of this new technique which permits the use of small routes of approach, perpendicular to the expansion area, thus, providing an excellent view with a minimal amount of trauma. The method prevents complications such as dehiscence with prosthesis exposure and hematomas. A precise dissection of the necessary area can be achieved by this technique, a process facilitated by the continuous expansion and bulging of the dissected area obtained by controlled injection of carbon dioxide. The disadvantages are the high cost of the procedure and the long surgical time. The authors emphasize the need for future rationalization of the indications and for an appropriate methodology for the procedure.


Assuntos
Queimaduras/cirurgia , Endoscopia , Expansão de Tecido/métodos , Pré-Escolar , Feminino , Humanos
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