Interleukin-10 functions in vitro and in vivo to inhibit bacterial DNA-induced secretion of interleukin-12.

Bacterial DNA (bDNA) has a number of biologic properties, including the ability to induce interleukin-12 (IL-12) production by macrophages. We studied the role of the regulatory cytokine IL-10 as a potential inhibitor of bDNA-induced IL-12 production. IL-10 concentrations as low as 0.3 ng/ml profoundly inhibited bDNA-induced macrophage IL-12 production as measured by Elispot analysis of IL-12 p40-secreting cells. Additionally, we found that IL-10 inhibited bDNA-induced IL-12 secretion by the macrophage cell lines J774 and RAW 264. Preincubation of splenic adherent cells with IL-10 markedly reduced bDNA-induced transcription of IL-12 p40 mRNA. Interestingly, after 2 h of exposure, bDNA also induces transcription of IL-10 mRNA by splenic adherent cells. The importance of IL-10 in the in vivo regulation of bDNA-induced cytokine secretion was illustrated by the response of mice with disrupted IL-10 genes (IL-10 ko mice) to i.v. bDNA challenge. Compared to +/+ mice, IL-10 knockout (ko) mice exhibited increased numbers of IL-12 and interferon-gamma (IFN-gamma)-secreting cells following either single or repeated challenge with bDNA. These findings indicate that IL-10 plays a key role in regulating bDNA-induced production of inflammatory cytokines.

[The morphological substrate and histogenesis of systemic and ocular atherosclerosis]. / Substratul morfologic si histogeneza aterosclerozei sistemice si oculare.

The atherosclerosis is an complex pathologic process with thrombosis and vessels obstructions. At the cerebral level, atherosclerosis determines lesions especially at the Willis circle vessels, and his peripheral ramifications (ophthalmic artery). The authors presents the atherosclerotic lesions at the level of ocular vessels, and their consequence at the central retinal artery, and other ocular vessels.

[Laser treatment in diabetic retinopathy]. / Tratamentul laser în retinopatia diabetica.

1050 eyes with diabetic retinopathy (670 preproliferatives retinopathies, 380 proliferatives retinopathies), was treated by laser argon photocoagulation. The laser photocoagulation is the election therapeutical method for the zones of neovascular perfusion, after angiofluorographic diagnosis. It is necessary to know very well the therapeutical indications and the correct technique adopted to each type of lesions. Also is necessary to obtain an equilibrium of the diabetic, especially in limitation of high variation of glycemia. In this study 20% of treated cases, was repeated the photocoagulation, and 10% of cases presented complications: vitreous hemorrhages, neovascular glaucoma, retinal detachment.