Assuntos
Membranas , Microssomos Hepáticos , Proteínas/análise , Animais , Ácidos e Sais Biliares/farmacologia , Centrifugação com Gradiente de Concentração , Eletroforese Descontínua , Lipídeos/análise , Masculino , Membranas/análise , Microscopia Eletrônica , Microssomos Hepáticos/efeitos dos fármacos , Peso Molecular , Ratos , SolubilidadeAssuntos
Colesterol/metabolismo , Fígado/metabolismo , Lipídeos de Membrana/metabolismo , Animais , Retículo Endoplasmático/metabolismo , Complexo de Golgi/metabolismo , Técnicas In Vitro , Lanosterol/metabolismo , Fígado/ultraestrutura , Masculino , Microssomos Hepáticos/metabolismo , Ratos , Esqualeno/metabolismo , Esteróis/biossínteseRESUMO
A single dose of [1-14C] or [U-14C]glycine or L-[1-14C] or [U-14C]valine was injected intraperitoneally into 1- and 3-week-old chicks. Continuous automatic determination of respiratory 14CO2 and determination of 14C in excreta and uric acid were carried out for 8 h. Computer-aided analysis of the kinetics of respiratory 14CO2 demonstrated two exponentially decreasing components, 'fast 14CO2' and slow 14CO2'. Fast 14CO2 production represents the immediate oxidation and/or decarboxylation of the 14C-labeled amino acid. The half-lives differed according to the nature of the 14C-labeled amino acid but not to the age of the chick. The size of the acetyl-CoA pool labeled by [14C]glycine was between 10 and 15 mmol/100 g of chick. Metabolic partition of a circulating plasma free amino acid between oxidation and retention could be quantified by an isotopic index.
Assuntos
Acetilcoenzima A/metabolismo , Aminoácidos/metabolismo , Respiração , Animais , Animais Recém-Nascidos , Dióxido de Carbono , Radioisótopos de Carbono , Galinhas , Glicina/metabolismo , Oxirredução , Ácido Úrico/metabolismo , Valina/metabolismoRESUMO
Analysis of the respiratory 14CO2 of growing chicks injected intraperitoneally with [1-14C] or [U-14C]glycine or valine has shown the presence of a delayed 'slow 14CO2' exponential component. The half-lives, about 3 h, are independent of the nature of the amino acid and of the age (1-3 weeks) of the animal. As supported by investigations in cycloheximide-treated chicks of the oxidative catabolism of glycine, valine and acetate, slow 14CO2 is significantly associated with the degradation of short-lived proteins.