Screening for genital human papillomavirus: results from an international validation study on human papillomavirus sampling techniques.

The objective of this study was to determine the validity of human papillomavirus (HPV) detection using exfoliated cervical cells compared with cervical biopsy specimens in women with normal cervix and to assess whether HPV detection rates using exfoliated cells is dependent on the number and order in which cervical scrapes are taken. Women undergoing hysterectomy for reasons other than cervical cancer were recruited in three hospitals in countries with varying risks of cervical cancer. After informed consent and at the time of surgery, three consecutive cervical scrapes were taken as well as four biopsy specimens, one in each of the quadrants around the cervical os. In this study, 331 women were recruited and provided 992 cell samples and 1324 biopsy samples. All scrapes and a sample of biopsy specimens (n = 103) were tested by polymerase chain reaction enzyme immunoassay using a general primer (GP5+/ bio6+). Type-specific tests were performed for 14 HPV types at the subpicogram level in one test and individually. Positive samples were verified using Southern blot hybridization. The prevalence of HPV DNA was 6.3% in cervical cells. Of 19 HPV positive samples in the scrapes, 17 were confirmed in the biopsy specimens. The agreement, as measured by the Kappa statistic, was 0.90 (P < 0.0001). The concordance in detecting HPV infection between scrapes and biopsy specimens was 97.5%, and the concordance in categorizing the samples as negatives was 94.4%. These values were unchanged when the order in which scrapes were taken was compared. Among women without cervical cancer, HPV DNA detection rates do not vary if exfoliated cells or random biopsy specimens are taken as the primary testing specimen. Screening programs based on highly sensitive HPV DNA detection technology in cell scrapes should expect a minimal underdetection.

Total and unbound cytosolic estrogen and progesterone receptors in myometrium and fibroid after gonadotropin-releasing hormone agonist treatment.

OBJECTIVE: To study the changes in bound and unbound cytosolic estrogen receptor (ER) and progesterone receptor (PR) in fibroid and myometrium after treatment with gonadotropin-releasing hormone agonists (GnRH-a). DESIGN: Prospective randomized study. SETTING: A tertiary institutional hospital. PATIENTS: Thirty premenopausal women with normal regular menstrual cycles and uterine fibroids. INTERVENTIONS: Patients were randomized to receive buserelin acetate for 3 months (n = 20) or no treatment (n = 10) before hysterectomy. MAIN OUTCOME MEASURES: Bound and unbound ER and PR. RESULTS: In nontreated women, values of unbound ER and PR in fibroid were higher than in myometrium. There were no differences in total receptors. The total and unbound ER in myometrium was higher in treated than in nontreated women. There were no differences in fibroid. CONCLUSIONS: The higher content of unbound hormone receptors in fibroid correlates with an exacerbation of the hormone dependence of this tissue compared with myometrium. The increase of total and unbound ER in myometrium after treatment suggests a higher level of synthesis or a lower level of degradation of receptors in this tissue.

Metastatic cancer spread at the laparoscopic puncture site. Report of a case in a patient with carcinoma of the ovary. Case report.

The aim of this study is to draw attention to the possibility of the occurrence of a metastatic spread at the laparoscopic entrance site in patients suffering from a carcinoma of the ovary. This event has rarely been mentioned in medical literature before. A case is presented here in which a localized tumoral lesion appeared after a laparoscopic staging study in the abdominal wall, exactly at the site used for the introduction of the accessory laparoscopic trocar in a patient suffering from a carcinoma of the ovary.