RESUMO
27 skin allografts were transplanted in duplicate to 13 rabbits defective in the sixth component (C'6) of complement (C'). 14 were rejected within the normal period of time and 9 only after a significant delay. In four grafts, no rejection was observed. Grafts exchanged between C' active and C' defective littermates tended to persist viable longer on the C' defective partners. It is concluded that in a species of higher vertebrates, the rabbit, allograft rejection can proceed in the absence of C'6 and the biological functions depending on it. This includes the cytotoxic function of complement. However, the prolonged survival in C'6 defective rabbits of some allografts strongly suggests that two pathways may be operative in the rejection reaction. The results are consistent with the view that a mechanism independent of C'6 was alone fully effective in the majority of the donor-host combinations. A C'6-dependent mechanism became influential only in some specific donor-host combinations.
Assuntos
Reações Antígeno-Anticorpo , Proteínas do Sistema Complemento , Transplante de Pele , Imunologia de Transplantes , Animais , Antígenos de Grupos Sanguíneos , Galinhas , Cães , Isoanticorpos , Coelhos , Ratos , Transplante HomólogoRESUMO
The survival of rat skin allografts was significantly increased by repeated intraperitoneal injections of 2.5 mg Valium (diazepam) into hosts. In addition, in vivo pretreatment of the donor animals at this dose level decreased the intensity of the recipients' immune responses to skin allografts. In vitro exposure of skin grafts to Valium also prolonged allograft survival.
Assuntos
Diazepam/farmacologia , Rejeição de Enxerto/efeitos dos fármacos , Transplante de Pele , Animais , Diazepam/administração & dosagem , Injeções Intraperitoneais , Ratos , Ratos Endogâmicos Lew , Transplante HomólogoAssuntos
Formação de Anticorpos , Reação Enxerto-Hospedeiro/efeitos dos fármacos , Hipersensibilidade/imunologia , Imunossupressores/farmacologia , Piridinas/farmacologia , Transplante de Pele , Imunologia de Transplantes/efeitos dos fármacos , Animais , Azatioprina/farmacologia , Cães , Feminino , Rejeição de Enxerto , Hemaglutinação , Camundongos , Camundongos Endogâmicos C57BL , Pirimidinas/toxicidade , Ratos , Ovinos , Ácidos Sulfênicos/farmacologia , Cauda , Transplante HomólogoAssuntos
Tecido Adiposo/efeitos dos fármacos , Sistema Fagocitário Mononuclear/efeitos dos fármacos , Silicones/farmacologia , Glândulas Suprarrenais/efeitos dos fármacos , Animais , Feminino , Injeções Intraperitoneais , Injeções Subcutâneas , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Tecido Linfoide/efeitos dos fármacos , Camundongos , Ovário/efeitos dos fármacos , Pâncreas/efeitos dos fármacos , Silicones/administração & dosagem , Baço/efeitos dos fármacosAssuntos
Silicones/metabolismo , Tecido Adiposo/efeitos dos fármacos , Glândulas Suprarrenais/efeitos dos fármacos , Animais , Carbono , Feminino , Histiócitos/efeitos dos fármacos , Injeções Intraperitoneais , Injeções Subcutâneas , Linfonodos/efeitos dos fármacos , Camundongos , Pericárdio/efeitos dos fármacos , Silicones/farmacologiaAssuntos
Transplante de Pele , Pele/irrigação sanguínea , Animais , Tamanho do Órgão , Ratos , Transplante AutólogoAssuntos
Próteses e Implantes , Silicones/metabolismo , Animais , Mama , Estética , Face , Feminino , Cobaias , Haplorrinos , Humanos , Técnicas In Vitro , Glândulas Mamárias Animais , Camundongos , Coelhos , Ratos , Silicones/farmacologia , Cirurgia PlásticaRESUMO
An operating table stand incorporating a rotatable mechanical surface with a motorized focusing stage carrier was designed for a standard stereomicroscope. This can be used for microsurgical experiments and practicing microsurgical techniques in small laboratory animals.
Assuntos
Laboratórios , Microscopia/instrumentação , Microcirurgia/instrumentação , Animais , PesquisaRESUMO
The stereomicroscopic appearance of the vasculature and circulation of groin flaps, with their epigastric pedicles divided at various time intervals, is an early diagnostic indication of flap viability. A simple and comprehensive training model, with characteristic vascular and flow changes within a single flap, is provided in rats, with pedicles transected at three to four days after the flap is raised.
Assuntos
Sobrevivência de Enxerto , Retalhos Cirúrgicos , Animais , Masculino , Microscopia , Ratos , Ratos Endogâmicos , Pele/irrigação sanguíneaRESUMO
This study entailed direct stereomicroscopic observations of rat abdominal island flaps and the sequence of vascular events that occurred during and after occlusion by clamping of the individual femoral vessels for varying time intervals of up to 16 hours. The vascular changes and the status of the circulation in the flap vasculature, including the timing of complete hemal stasis and resumption of flow, following the application and subsequent release of the vessel clamp were observed and correlated with flap viability and behavior. This study also included a grading system of the vascular changes observed under a stereomicroscope. From these findings, it is suggested that in vivo stereomicroscopy may serve as a new and simple clinical tool for the early diagnosis of thrombosis at the site of microvascular anastomosis of a free flap transfer and may define time limits during which the anastomosis can be successfully corrected and the flap salvaged.
Assuntos
Sobrevivência de Enxerto , Microscopia/métodos , Retalhos Cirúrgicos , Animais , Feminino , Microcirculação/fisiologia , Ratos , Ratos EndogâmicosRESUMO
A lacerated fresh leaf is introduced as a model for developing and refining microvascular skills. Being friable, this plant tissue exaggerates damage due to technical errors, therefore improving a trainee's skills.
Assuntos
Microcirurgia , Procedimentos Cirúrgicos Vasculares/educação , Animais , Plantas , RatosRESUMO
Free groin flaps taken from rats were preserved by refrigeration at 4 degrees C in either lactated Ringers solution or tissue culture medium for various periods of time. The results indicate that a high survival rate can be expected at periods up to 72 hours, but there was no success in preserving flaps longer than 72 hours. These preliminary experimental findings suggest that, clinically, a high survival rate can be achieved in free flaps following excision from donor sites even if the microvascular transfer must be postponed for a period up to but not exceeding 48 hours.