Autologous transplantation of muscle-derived CD133+ stem cells in Duchenne muscle patients.

Duchenne muscular dystrophy (DMD) is a lethal X-linked recessive muscle disease due to defect on the gene encoding dystrophin. The lack of a functional dystrophin in muscles results in the fragility of the muscle fiber membrane with progressive muscle weakness and premature death. There is no cure for DMD and current treatment options focus primarily on respiratory assistance, comfort care, and delaying the loss of ambulation. Recent works support the idea that stem cells can contribute to muscle repair as well as to replenishment of the satellite cell pool. Here we tested the safety of autologous transplantation of muscle-derived CD133+ cells in eight boys with Duchenne muscular dystrophy in a 7-month, double-blind phase I clinical trial. Stem cell safety was tested by measuring muscle strength and evaluating muscle structures with MRI and histological analysis. Timed cardiac and pulmonary function tests were secondary outcome measures. No local or systemic side effects were observed in all treated DMD patients. Treated patients had an increased ratio of capillary per muscle fibers with a switch from slow to fast myosin-positive myofibers.

Early postoperative seizures and endovenous phenytoin. Preliminary clinical data.

Early seizures represent a major complication in the post operative course of patients operated on for supratentorial tumors or AVMs. The real effectiveness of the AEDs prophylaxis to reduce the occurrence of post operative seizures is controversial. We proposed a prophylactic treatment with endovenous PHT consisting of two infusions of PHT (mean dosage of 18 mg/kg; mean time of 1 hr) perioperatively and during the first postoperative day. The interruption of the previous oral anticonvulsant treatment is not required. The endovenous route should permit a rapid reach of the therapeutical range. Sixty-six patients were treated. Fifty-one patients received two infusions and 15 patients only one infusion. The serum concentration of PHT performed at 24 hrs of operation was in most of patients (more than 80%) in the lower part of the therapeutical range while at 24 hrs of the second infusion was in the higher part or over the range. The overall prevalence of seizures was 10.6%. In the first group the incidence was 7.8%, in the second one was 20%. All the seizures appeared within 48 hrs of the operation. All the patients in the first group had single seizures, 2 patients of the second one experienced two seizures. No status epilepticus was observed. Alteration of consciousness and mild hypotension were the most common side effects. They never required major measurements and were mild, transient and completely reversible. We are starting with a randomized study based on a larger sample of patients which will allow a more reliable statistical analysis.

[Guidelines for the management of hypertensive crises and simple blood pressure rise. Literature review and clinical experience]. / Linee guida della terapia delle crisi ipertensive e dei rialzi pressori. Rassegna della letteratura ed esperienza personale.

Hypertension is a common clinical problem in the Emergency Department. Beside homologated therapeutical approaches it is necessary in primis to consider the problem in a clinical context and in an appropriate nosographic scheme in order to provide a rational approach to the treatment. In this paper the authors review the nosographic and therapeutical approaches previously reported in literature and then state their proposals derived by their daily experience at the Emergency Department. In authors' opinion it is of primary importance to distinguish between hypertensive crises and simple blood pressure rise. For this end it seems useful to define four syndromic classes: A. Real Hypertensive Crises: 1-Hypertensive Emergencies, 2-Hypertensive Urgencies. B. Simple Blood Pressure Rise: 1-Stable Uncomplicated Hypertension, 2-Transient Hypertension. According to this classification the majority of patients referring to Emergencies Departments for elevated blood pressure can be included in the last two classes. The authors suggest a step-by-step approach to the treatment beginning with benzodiazepines, loop diuretics, beta-blockers or clonidine or ACE inhibitors or calcium channel blockers; In an elevated percentage of cases benzodiazepines alone are effective in appropriately lowering blood pressure.

Awake neurosurgery: an update.

Intraoperative brain mapping has the goal of aiding with maximal surgical resection of brain tumors while minimizing functional sequelae. Retrospective randomized studies on large populations have shown that this technique can optimize the surgical approach while reducing postoperative morbidity. During direct electrical stimulation of the language areas adjacent to the tumor, the patient should be collaborative and be able to speak to participate in language testing. Different anesthesiological protocols have been proposed to allow intraoperative brain mapping, which range from local anesthesia to conscious sedation or general anesthesia, with or without airway instrumentation. The most common intraoperative complications are seizure, respiratory depression, and patients' stress and discomfort. Since awake craniotomy carries both benefits and potential risks, the following factors are crucial in the management of patients: 1) careful selection of the patients and 2) communication between the anesthesiological and surgical teams. To date, there remains no consensus about the optimal anesthesiological regimen to use. Only prospective, multicentre randomized studies focused on evaluating the role of different anesthesiological techniques on intraoperative monitoring, postoperative deficits, and intraoperative complications can answer the question of which anesthesiological approach should be chosen when intraoperative brain mapping is requested.

[Sodium nitroprusside in neurosurgery. Clinical considerations]. / Il nitroprussiato di sodio in neurochirurgia. Considerazioni cliniche.

The authors present their experience with SNP as hypotensive drug in surgery at cerebral aneurysms. They stress the effectiveness of SNP which they use to control deep hypotensive in 22 cases. SNP induces hypotensive in few minutes, without depressing myocardial and renal functions. The authors present also some criteria at selection of patients (to be treated with SNP during surgery) and stress the importance of monitoring blood gases during SNP hypotensive, especially te pO2 arteriovenous gradient. Using SNP at the recommended doses, and hydroxycobalamin as preventive treatment, no CN- toxicity was noted. The authors think SNP is the drug of choice for inducing deep hypotension in neurosurgery.

Serum gastrin levels in patients with renal failure on maintenance haemodialysis and after successful kidney transplantation.

Chronic renal failure in man is associated with a hypergastrinaemia which is not corrected by haemodialysis. Serum gastrin concentrations were measured in 66 patients on maintenance haemodialysis and in 27 patients after successful kidney transplantation. The basal gastrin levels distinguished 2 groups of patients on maintenance haemodialysis: a first group with gastrin values less than or equal to 120 pg/ml (mean 58+/-29.88 pg/ml) and a second group with gastrin values greater than 120 pg/ml (mean 295+/-257 pg/ml). The onset of urine output after kidney transplantation was associated with a dramatic fall in gastrin concentration (3 patients studied in the early post-transplant period). 24 patients with stabilized transplant function had serum gastrin levels less than 120 pg/ml (mean 21.33+/- 17.29 pg/ml). The gastrin response to a standard protein meal depended on the basal gastrin concentration and was different in patients with renal failure on maintenance haemodialysis and those with a successful kidney transplantation.