RESUMO
The neuronal-specific G protein Gz is known to interact with a large variety of receptors for neurotransmitters and hormones. Fatty acylations on the N-terminus of the alpha subunit of Gz (alpha z) provide anchorage to the plasma membrane. Fatty acylation-deficient mutants of alpha z have previously been shown to exhibit altered signaling properties. Since the N-terminus of alpha z is likely to play a critical role in beta gamma binding, we examined the ability of these mutants to interact with beta gamma subunits by means of receptor-mediated stimulation of beta gamma-sensitive type II adenylyl cyclase. Our results indicate that lack of myristoylation, but not lack of palmitoylation, impaired the ability of alpha z to mediate receptor-induced release of beta gamma subunits.
Assuntos
Proteínas de Ligação ao GTP/metabolismo , Mutagênese Sítio-Dirigida , Receptores Opioides delta/fisiologia , Acilação , Toxina Adenilato Ciclase , Adenilil Ciclases/metabolismo , Animais , Células COS , AMP Cíclico/metabolismo , DNA Complementar , D-Penicilina (2,5)-Encefalina , Encefalinas/farmacologia , Proteínas de Ligação ao GTP/biossíntese , Proteínas de Ligação ao GTP/genética , Substâncias Macromoleculares , Ácido Mirístico , Ácidos Mirísticos/metabolismo , Neurônios/metabolismo , Ácido Palmítico/metabolismo , Mutação Puntual , Receptores Opioides delta/biossíntese , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Transdução de Sinais , Transfecção , Fatores de Virulência de Bordetella/farmacologiaRESUMO
A case of herpes gestationis (HG) and a hormone producing tumour in a woman is presented. The disease had a fulminant course and the patient deceased within months. Fifteen years ago a hydatidiform mole had been removed. Only rarely has HG been associated with hydatidiform mole and choriocarcinoma. We believe that our patient had a human chorionic gonadotrophin (HCG) producing germ cell tumour. Although extremely seldom encountered, the association of HG to neoplasms should be kept in mind.
Assuntos
Gonadotropina Coriônica/metabolismo , Neoplasias Embrionárias de Células Germinativas/patologia , Penfigoide Gestacional/patologia , Complicações na Gravidez/patologia , Neoplasias Retroperitoneais/patologia , Dermatopatias Vesiculobolhosas/patologia , Pele/patologia , Adulto , Feminino , Humanos , Neoplasias Embrionárias de Células Germinativas/metabolismo , Gravidez , Neoplasias Retroperitoneais/metabolismoAssuntos
Clima , Dermatite Atópica/prevenção & controle , Habitação , Microclima , Adolescente , Adulto , Alérgenos , Criança , Pré-Escolar , Feminino , Humanos , Umidade , Masculino , Pessoa de Meia-Idade , VentilaçãoAssuntos
Doenças do Ducto Colédoco/cirurgia , Cistos/cirurgia , Criança , Feminino , Humanos , Masculino , Métodos , Pessoa de Meia-IdadeRESUMO
Skin blood flow was measured by the laser Doppler technique in lesional and clinically normal skin of 10 diabetic patients with necrobiosis lipoidica during and after treatment with 40 mg acetylsalicylic acid (ASA) daily. The measurements showed that the blood flow during ASA treatment was significantly decreased in the central lesional skin without changes in the peripheral part of the lesions and normal skin. In view of these findings we suggest that low-dose ASA may not be the best treatment for necrobiosis lipoidica.
Assuntos
Aspirina/efeitos adversos , Necrobiose Lipoídica/fisiopatologia , Pele/irrigação sanguínea , Adolescente , Adulto , Aspirina/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Necrobiose Lipoídica/tratamento farmacológico , Fluxo Sanguíneo Regional/efeitos dos fármacosRESUMO
In 27 patients with vitiligo punch biopsies from vitiliginous skin to normal skin and vice versa were performed. Of the vitiligo grafts 69% showed donor dominance and 31% receptor dominance. When normal skin was transplanted 74% showed receptor dominance and 26% donor dominance. In 13 patients (52%) blood screening pointed towards an autoimmune disease.
Assuntos
Transplante de Pele , Vitiligo , Adolescente , Adulto , Idoso , Autoanticorpos/análise , Biópsia , Criança , Feminino , Seguimentos , Humanos , Masculino , Microssomos/imunologia , Pessoa de Meia-Idade , Células Parietais Gástricas/imunologia , Pele/patologia , Tireoglobulina/imunologia , Glândula Tireoide/imunologia , Vitiligo/imunologia , Vitiligo/patologiaRESUMO
A 74-year-old woman with a chronic, severe, erosive lichen planus on the tongue, buccal mucosa and toes was treated successfully with dapsone. Previous therapy had failed, Partly due to side effects.
Assuntos
Dapsona/uso terapêutico , Líquen Plano/tratamento farmacológico , Idoso , Dapsona/administração & dosagem , Feminino , Dermatoses do Pé/tratamento farmacológico , Humanos , Doenças da Boca/tratamento farmacológico , Mucosa Bucal , Dedos do Pé , Doenças da Língua/tratamento farmacológicoRESUMO
The occurrence of house dust mites was measured in the homes of 12 patients with the scaly skin disease, psoriasis. The median number of mites in the mattress dust and floor dust was 27 (range 10-153)/0.1 g and 56 (range 12-114)/0.1 g respectively, which is significantly higher than the background pollution of house dust mites in normal Danish homes. The results support the idea that the amount of mites in our homes not only depend on relative air humidity but also on the production of dandruff.
Assuntos
Poeira , Ácaros , Psoríase/patologia , Pele/patologia , Adulto , Animais , Leitos , Dermatite/etiologia , Dermatite/patologia , Poeira/efeitos adversos , Feminino , Habitação , Humanos , Umidade , Hipersensibilidade Imediata/etiologia , Hipersensibilidade Imediata/patologia , Masculino , Pessoa de Meia-Idade , Ácaros/imunologiaRESUMO
An analysis of the month of birth in 210 patients with atopic dermatitis and a positive prick test towards the house dust mite (Dermatophagoides pteronyssinus) showed a significantly higher prevalence of birth in the interval May to November when compared with the expected distribution (p less than 0.01). A subdivision of the AD patients due to the concomitant presence of asthma showed no differences as the relative risk was nearly the same in the two subgroups. This observation indicates that a consistent seasonal preference of birth in patients with atopic dermatitis only applies to the skin disease and not to coexistent asthma.
Assuntos
Asma/etiologia , Dermatite Atópica/etiologia , Ácaros/imunologia , Estações do Ano , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Estudos Retrospectivos , Fatores de RiscoRESUMO
The occurrence of house dust mites (Dermatophagoides spp) was investigated in the homes of 26 patients with atopic dermatitis (AD), 20 patients with psoriasis and 41 randomly selected homes in Arhus, Denmark. The AD patients with moderate to severe eczema had an increased concentration of mites (median 85 mites/0.1 g mattress dust) compared with the controls (median 8 mites/0.1 g mattress dust). The higher exposure to house dust mites corresponded to a relative risk of 4.6 and a clear dose-response relationship between exposure and disease could be demonstrated. Our results illustrate a clear association between moderate to severe atopic dermatitis and increased exposure to house dust mites in the patients' homes, and support the hypothesis that mite antigens could be an aetiological factor in atopic dermatitis.
Assuntos
Dermatite Atópica/etiologia , Ácaros/imunologia , Adolescente , Adulto , Alérgenos/imunologia , Animais , Criança , Poeira , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de RiscoRESUMO
The frequency of type-I reactions (contact urticaria) to plaice, whiting, common dab, witch, codfish, Norwegian haddock, herring and halibut has been examined in 26 individuals; 23 of these carried through all the investigations. 65% of the volunteers had a positive scratch patch test to one or more species of fish. All 20 min closed patch tests were negative. 5 of the volunteers were atopics. They showed about twice as many positive scratch patch tests when compared to the non-atopics. The investigation shows that the "fish allergen" is destroyed by cooking but not by freezing. The possibility of common and specific fish allergens is discussed.
Assuntos
Dermatite de Contato/etiologia , Dermatite Ocupacional/etiologia , Peixes , Indústria de Processamento de Alimentos , Urticária/etiologia , Animais , Produtos Pesqueiros/efeitos adversos , Pesqueiros , HumanosRESUMO
A case of toxic hepatitis caused by combination therapy with methotrexate and etretinate in the treatment of severe psoriasis is presented in a 47-year-old woman. The patient had received methotrexate alone for more than 10 years without any signs of severe liver damage. The liver status had been controlled by consecutive liver biopsies. The treatment was discontinued and after 2 months the liver parameters became normal.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/etiologia , Metotrexato/efeitos adversos , Psoríase/tratamento farmacológico , Tretinoína/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/diagnóstico , Doença Hepática Induzida por Substâncias e Drogas/patologia , Quimioterapia Combinada , Feminino , Humanos , Testes de Função Hepática , Metotrexato/uso terapêutico , Pessoa de Meia-Idade , Tretinoína/uso terapêuticoRESUMO
Non-traumatic extra-hepatic rupture (NTEHR) of the bile system into free peritoneal cavity is a well-known condition. Usually the gall-bladder is the site of perforation, but non-traumatic perforation of the common bile duct and the hepatic duct occurs. For the hepatic duct it is extremely rare and has so far been reported in only 7 cases in adults (Christensen, Baekgaard & Blichert-Toft, 1977; MacWilliams, 1912; Reich, 1942). A review of the literature reveals, that non-traumatic intra-hepatic rupture (NTIHR) in the biliary tree with perforation into free peritoneal cavity has apparently never been reported before. The purpose of this paper is to report two such cases with recovery.