RESUMO
The influence of non-opsonized and opsonized S. aureus 2879M and E. coli 321 strains on the total strength of interaction between the endothelial cell and neutrophil during the docking process was studied using in vitro model of experimental septicemia. We observed a decrease in the force and work of adhesion between receptors of neutrophils and endothelial cells under the influence of non-opsonized strains and further decrease in the affinity of single interactions between cells under the influence of opsonized S. aureus, which was compensated by an increase in the number of contacts, as well as an increase in the force of adhesion under the influence of opsonized E. coli compared to non-opsonized bacteria, which remained below the control level, while adhesion work reaches the control level. Thus, opsonization of S. aureus aggravates the "immunological uncoupling" between neutrophils and endothelial cells, while opsonization of E. coli reduces the pathological effect compared to non-opsonized bacteria.
Assuntos
Células Endoteliais , Escherichia coli , Neutrófilos , Sepse , Staphylococcus aureus , Neutrófilos/imunologia , Neutrófilos/metabolismo , Escherichia coli/imunologia , Staphylococcus aureus/imunologia , Staphylococcus aureus/patogenicidade , Sepse/imunologia , Sepse/microbiologia , Sepse/metabolismo , Sepse/patologia , Células Endoteliais/imunologia , Células Endoteliais/metabolismo , Células Endoteliais/microbiologia , Humanos , Fagocitose , Adesão Celular/imunologia , Proteínas Opsonizantes/metabolismo , Proteínas Opsonizantes/imunologia , Aderência Bacteriana , AnimaisRESUMO
The incubation of quantum dots with lymphocytes induced significant changes in all morphometric characteristics of these cells. Protein crown formed on the surface of quantum dots after incubation with the serum consists mainly of transport proteins, immunoglobulins, blood coagulation proteins, and kininogens. Protein crown changes the morphometric characteristics of cells: in the case of incubation with quantum dots that have low-molecular-weight coating, a shift towards control parameters (cells without exposure) was observed; on the contrary, after incubation with quantum dots that have a high-molecular-weight coating, the differences from the control became more pronounced. It can be hypothesized that protein crown provokes autoagression of lymphocytes against each other and against platelets.
Assuntos
Linfócitos/ultraestrutura , Pontos Quânticos , Adulto , Humanos , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Adulto JovemRESUMO
Thanks to the modification of the force spectroscopy method, when a neutrophil is fixed on the tip, and an endotheliocyte culture is grown on the substrate, the exact indicators of the adhesion force and adhesion work between cells have been investigated. The high variability of adhesion contacts in different donors associated with different expression profiles of neutrophils. It was found by flow cytometry that the EA.hy926 cell line actively expresses VCAM-1, as well as P- and E-selectin under the Staphylococcus aureus influence after 60 min of co-incubation. At the same time, the integral indicators of the adhesion force and adhesion work in the "neutrophil - endothelial cell" interaction were significantly inhibited by S. aureus in all studied donors. Since the VCAM-1 receptor is not involved in the adhesion bonds between neutrophils and endothelial cells, the suppression of the interaction is associated with the inhibition of P- and E-selectins, but direct receptors removal from the endothelial cells surface of the EA.hy926 cell line does not occur. Escherichia coli causes multidirectional effects in the system of interaction "neutrophil - endothelial cell", depending on the expression profile of the donor's neutrophils. However, the cumulative effect of interaction from all donors shows that in general, under the influence of E. coli, there is an increase in adhesion force and a suppression of adhesion work.