RESUMO
Mice that are deficient in either the Pms2 or Msh2 DNA mismatch repair genes have microsatellite instability and a predisposition to tumours. Interestingly, Pms2-deficient males display sterility associated with abnormal chromosome pairing in meiosis. Here mice deficient in another mismatch repair gene, Mlh1, possess not only microsatellite instability but are also infertile (both males and females). Mlh1-deficient spermatocytes exhibit high levels of prematurely separated chromosomes and arrest in first division meiosis. We also show that Mlh1 appears to localize to sites of crossing over on meiotic chromosomes. Together these findings suggest that Mlh1 is involved in DNA mismatch repair and meiotic crossing over.
Assuntos
Troca Genética , Reparo do DNA , Proteínas Fúngicas/genética , Meiose/genética , Proteínas Adaptadoras de Transdução de Sinal , Sequência de Aminoácidos , Animais , Sequência de Bases , Núcleo Celular/química , Núcleo Celular/genética , Epididimo/patologia , Feminino , Proteínas Fúngicas/química , Infertilidade Feminina/genética , Infertilidade Masculina/genética , Masculino , Camundongos , Camundongos Transgênicos , Dados de Sequência Molecular , Proteína 1 Homóloga a MutL , Mutação , Oócitos/química , Oócitos/patologia , Proteínas de Saccharomyces cerevisiae , Espermatócitos/química , Testículo/patologiaRESUMO
Germline mutations in the human MSH2, MLH1, PMS2 and PMS1 DNA mismatch repair (MMR) gene homologues appear to be responsible for most cases of hereditary non-polyposis colorectal cancer (HNPCC; refs 1-5). An important role for DNA replication errors in colorectal tumorigenesis has been suggested by the finding of frequent alterations in the length of specific mononucleotide tracts within genes controlling cell growth, including TGF-beta receptor type II (ref. 6), BAX (ref. 7) and APC (ref. 8). A broader role for MMR deficiency in human tumorigenesis is implicated by microsatellite instability in a fraction of sporadic tumours, including gastric, endometrial and colorectal malignancies. To better define the role of individual MMR genes in cancer susceptibility and MMR functions, we have generated mice deficient for the murine homologues of the human genes MLH1, PMS1 and PMS2. Surprisingly, we find that these mice show different tumour susceptibilities, most notably, to intestinal adenomas and adenocarcinomas, and different mutational spectra. Our results suggest that a general increase in replication errors may not be sufficient for intestinal tumour formation and that these genes share overlapping, but not identical functions.
Assuntos
Adenosina Trifosfatases , Proteínas de Transporte , Enzimas Reparadoras do DNA , Proteínas de Ligação a DNA , Proteínas Fúngicas/genética , Neoplasias Intestinais/genética , Mutação , Proteínas de Neoplasias/deficiência , Proteínas/genética , Proteínas Adaptadoras de Transdução de Sinal , Animais , Reparo do DNA/genética , Replicação do DNA/genética , Suscetibilidade a Doenças , Intestinos/anatomia & histologia , Intestinos/patologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Camundongos Mutantes , Repetições de Microssatélites , Endonuclease PMS2 de Reparo de Erro de Pareamento , Dados de Sequência Molecular , Proteína 1 Homóloga a MutL , Proteínas MutL , Proteínas de Neoplasias/genética , Proteínas Nucleares , Especificidade de Órgãos , Neoplasias Cutâneas/genéticaRESUMO
Analysis of two human familial cancer syndromes, hereditary nonpolyposis colorectal cancer and familial adenomatous polyposis, indicates that mutations in either one of four DNA mismatch repair gene homologues or the adenomatous polyposis coli (APC) gene, respectively, are important for the development of colorectal cancer. To further investigate the role of DNA mismatch repair in intestinal tumorigenesis, we generated mice with mutations in both Apc and the DNA mismatch repair gene, Pms2. Whereas Pms2-deficient mice do not develop intestinal tumors, mice deficient in Pms2 and heterozygous for Min, an allele of Apc, develop approximately three times the number of small intestinal adenomas and four times the number of colon adenomas relative to Min and Pms2+/-;Min mice. Although Pms2 deficiency clearly increases adenoma formation in the Min background, histological analysis indicated no clear evidence for progression to carcinoma.
Assuntos
Adenoma/genética , Adenosina Trifosfatases , Proteínas do Citoesqueleto/genética , Enzimas Reparadoras do DNA , Proteínas de Ligação a DNA , Pólipos Intestinais/genética , Proteínas/fisiologia , Proteína da Polipose Adenomatosa do Colo , Animais , Reparo do DNA , Camundongos , Camundongos Knockout , Camundongos Mutantes , Repetições de Microssatélites/genética , Endonuclease PMS2 de Reparo de Erro de Pareamento , Neoplasias Experimentais , Polimorfismo Conformacional de Fita SimplesRESUMO
The DNA mismatch repair (MMR) system in mammalian cells not only serves to correct base mispairs and other replication errors, but it also influences the cellular response to certain forms of DNA damage. Cells that are deficient in MMR are relatively resistant to alkylation damage because, in wild-type cells, the MMR system is thought to promote toxicity via futile repair of alkylated mispairs. Conversely, MMR-deficient cells are sensitive to UV light, possibly due to the requirement for MMR factors in transcription-coupled repair of active genes. MMR deficiency has been associated with familial and sporadic carcinomas of the colon and other sites, and so, we sought to determine the influence of MMR status on cellular response to ionizing radiation, an agent commonly used for cancer therapy. Fibroblast cell lines were established from transgenic mice carrying targeted disruptions of one of three MMR genes in mammalian cells: Pms2, Mlh1, or Msh2. In comparison to wild-type cell lines from related mice, the Pms2-, Mlh1-, or Msh2-nullizygous cell lines were found to exhibit higher levels of clonogenic survival following exposure to ionizing radiation. Because ionizing radiation generates a variety of lesions in DNA, the differences in survival may reflect a role for MMR in processing a subset of these lesions, such as damaged bases. These results both identify a new class of DNA-damaging agents whose effects are modulated by the MMR system and may help to elucidate pathways of radiation response in cancer cells.
Assuntos
Dano ao DNA/genética , Reparo do DNA/genética , Proteínas de Ligação a DNA/genética , DNA/efeitos da radiação , Animais , Linhagem Celular/efeitos da radiação , Proteínas de Ligação a DNA/fisiologia , Fibroblastos/efeitos da radiação , CamundongosRESUMO
The tmpA600 mutation confers thymidylate synthase deficiency and thymidine auxotrophy to Dictyostelium discoideum. The tdrA600 mutation enhances transport of thymidine and thereby reduces the auxotrophic requirement of tmpA600 strains. The tmpA locus maps to linkage group III. The tdrA600 mutation is dominant and cosegregates with both linkage groups IV and VI, possibly because of a translocation between the two. The tdrA600 allele is sufficient to allow efficient incorporation of exogenous [3H]thymidine or [3H]uridine into TCA-precipitable material and to sensitize the cell to the nucleoside-analog inhibitor, 5-fluorodeoxyuridine. These properties make the tdrA mutation useful for studies requiring labelling of DNA or RNA in vivo.
Assuntos
Dictyostelium/genética , Mutação , Timidina/metabolismo , Timidilato Sintase/genética , Mapeamento Cromossômico , Ligação Genética , Fenótipo , PloidiasRESUMO
In the experimentally observed relationship between survival of colony-forming ability and the amount of exposure to ultraviolet light, two characteristics are generally found. First, sensitive and resistant components often show. Second, there is often a shouldered character to the survival. We present evidence that the first is largely due to the presence of active replication forks in the genome, and that the second is related to the operation of the recombinational repair system. We are able to describe our data in terms of a superposition of single and multiple-hit fractions and to show that the latter are greatly increased, in excision-repair-competent strains, by prevention of protein synthesis for 1 h prior to irradiation. Applying this analysis and treatment to a number of mutant strains enables us to make suggestions as to the interaction between recombinational and excision repair.
Assuntos
Reparo do DNA , Escherichia coli/efeitos da radiação , Exodesoxirribonuclease V , Exodesoxirribonucleases/genética , Genes Bacterianos , Recombinases Rec A/genética , Recombinação Genética , Raios UltravioletaRESUMO
We describe 22 new mutants of D. discoideum that are sensitive to DNA damage. These mutants were isolated on the basis of sensitivity to either temperature, gamma-rays, or 4-nitroquinolone-1-oxide (4NQO). The doses of gamma-rays, ultraviolet light (UV), and 4NQO required to reduce the survival of colony-forming ability of these mutants to 10% (D10) range from 2% to 100% of the D10s for the nonmutant, parent strains. For most of the mutants, those which are very sensitive to one agent are very sensitive to all agents tested and those which are moderately sensitive to one agent, are moderately sensitive to all agents tested. One mutant is sensitive only to 4NQO. Linkage relationships have been examined for 13 of these mutants. This linkage information was used to design complementation tests to determine allelism with previously characterized complementation groups affecting sensitivity to radiation. 4 of the new mutants fall within previously identified complementation groups and 3 new complementation groups have been identified (radJ, radK and radL). Other new loci probably also exist among these new mutants. This brings the number of characterized mutants of D. discoideum which are sensitive to DNA-damaging agents to 33 and the number of assigned complementation groups to 11.
Assuntos
Dano ao DNA , Reparo do DNA , Dictyostelium/genética , 4-Nitroquinolina-1-Óxido/toxicidade , Animais , Dictyostelium/efeitos dos fármacos , Dictyostelium/efeitos da radiação , Raios gama , Teste de Complementação Genética , Ligação Genética , Mutagênese , Raios UltravioletaRESUMO
The human DNA mismatch repair gene homologue hMSH2, on chromosome 2p is involved in hereditary non-polyposis colon cancer (HNPCC). On the basis of linkage data, a second HNPCC locus was assigned to chromosome 3p21-23 (ref. 3). Here we report that a human gene encoding a protein, hMLH1 (human MutL homologue), homologous to the bacterial DNA mismatch repair protein MutL, is located on human chromosome 3p21.3-23. We propose that hMLH1 is the HNPCC gene located on 3p because of the similarity of the hMLH1 gene product to the yeast DNA mismatch repair protein, MLH1, the coincident location of the hMLH1 gene and the HNPCC locus on chromosome 3, and hMLH1 missense mutations in affected individuals from a chromosome 3-linked HNPCC family.
Assuntos
Neoplasias Colorretais Hereditárias sem Polipose/genética , Reparo do DNA/genética , Mutação , Proteínas de Neoplasias/genética , Proteínas Adaptadoras de Transdução de Sinal , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteínas de Transporte , Células Cultivadas , Mapeamento Cromossômico , Cromossomos Humanos Par 3 , DNA de Neoplasias , Humanos , Células Híbridas , Dados de Sequência Molecular , Proteína 1 Homóloga a MutL , Proteínas Nucleares , Reação em Cadeia da Polimerase , Polimorfismo Genético , Roedores , Saccharomyces/genética , Homologia de Sequência de AminoácidosRESUMO
Using gene targeting in embryonic stem cells, we have derived mice with a null mutation in a DNA mismatch repair gene homolog, PMS2. We observed microsatellite instability in the male germline, in tail, and in tumor DNA of PMS2-deficient animals. We therefore conclude that PMS2 is involved in DNA mismatch repair in a variety of tissues. PMS2-deficient animals appear prone to sarcomas and lymphomas. PMS2-deficient males are infertile, producing only abnormal spermatozoa. Analysis of axial element and synaptonemal complex formation during prophase of meiosis I indicates abnormalities in chromosome synapsis. These observations suggest links among mismatch repair, genetic recombination, and chromosome synapsis in meiosis.