RESUMO
We investigated IgG transport across the gut of suckling opossums to see whether it is likely to be Fc gamma R-mediated. Enterocytes isolated from the proximal and distal regions of the small intestine of suckling aged 12-52 days, and reacted with indicator SRBC at pH 6.0 or 7.2, bound opossum IgG in rosette assays. Considerable overall variation was observed in the numbers of enterocytes forming rosettes. No binding was seen with rabbit IgG at these ages, or with opossum and rabbit IgG when enterocytes were obtained from opossums aged 55-73 days. Opossum anti-SRBC antibody (IgG) fed to sucklings at 52 days and earlier (but not later) could subsequently be detected in the serum. However, rabbit anti-SRBC antibody (IgG) could not be detected in the blood serum when fed to sucklings of any age. Fluorescent tracing of FITC-labelled opossum and rabbit IgG fed to suckling opossums, and of endogenous opossum IgG, pointed to transport of the homologous IgG occurring across gut enterocytes of the proximal region. These results suggest that IgG is recognised and transcytosed by specific Fc gamma Rs present on opossum enterocytes prior to weaning.
Assuntos
Imunoglobulina G/metabolismo , Intestino Delgado/imunologia , Gambás/imunologia , Fatores Etários , Animais , Animais Lactentes , Transporte Biológico Ativo , Imunofluorescência , Coelhos , Ratos , Ratos Wistar , Receptores de IgG/metabolismo , Especificidade da EspécieRESUMO
During the process of implantation, maternal spiral arteries within the decidua are invaded by trophoblast cells that adhere to and migrate along the luminal surface of the vascular endothelial cells. This phenomenon resembles the events that occur during the migration of neutrophils into an acute inflammatory site, therefore it is possible that similar mechanisms are involved. Indeed, previous observations have shown that endovascular trophoblast expresses the blood group-related antigen sialyl Le(x). In this study, we show, by immunohistology, the expression of both E- and P-selectin by vascular endothelial cells only in the decidua basalis and not in decidua parietalis. In contrast, ICAM-1 is expressed by all vascular endothelium throughout the decidua. Expression of VCAM-1 is variable at the implantation site, and is not expressed by vascular endothelial cells in decidua parietalis. Interestingly, we demonstrate the strong expression of a polysialylated form of NCAM by endovascular trophoblast. Our data suggests that vascular invasion by trophoblast is regulated by the expression of appropriate adhesion molecules which permit interaction between endovascular trophoblast and decidual endothelial cells.
Assuntos
Moléculas de Adesão Celular/fisiologia , Decídua/citologia , Implantação do Embrião , Endotélio Vascular/citologia , Cadeias alfa de Integrinas , Trofoblastos/citologia , Moléculas de Adesão Celular/análise , Moléculas de Adesão Celular Neuronais/análise , Moléculas de Adesão Celular Neuronais/fisiologia , Decídua/química , Decídua/fisiologia , Endotélio Vascular/química , Endotélio Vascular/fisiologia , Feminino , Humanos , Imuno-Histoquímica , Molécula 1 de Adesão Intercelular , Selectina-P , Glicoproteínas da Membrana de Plaquetas/análise , Glicoproteínas da Membrana de Plaquetas/fisiologia , Gravidez , Receptores de Antígeno muito Tardio/análise , Receptores de Antígeno muito Tardio/fisiologia , Trofoblastos/química , Trofoblastos/fisiologia , Molécula 1 de Adesão de Célula VascularRESUMO
During placentation the extravillous trophoblast (EVT) cells migrate through the decidua towards the maternal spiral arteries. The walls of the arteries are then destroyed by trophoblast resulting in an increased blood flow to the fetus. These EVT express HLA-G, HLA-E and HLA-C, an unusual combination of two non-classical and one classical MHC class I molecules. The decidua is infiltrated by distinctive uterine natural killer (NK) cells during the time of trophoblast invasion. These cells express a variety of receptors (CD94/NKG2, KIR and ILT) which are known to recognize HLA class I molecules. There is, therefore, a mechanism for molecular recognition of the placental trophoblast cells. The possible functional consequences of this uterine NK cell-trophoblast interactions are uncertain. One possible result is in an altered NK cell cytokine profile which modulates the invasive proclivity of the EVT. In this way placentation could be controlled.
Assuntos
Decídua/imunologia , Antígenos de Histocompatibilidade Classe I/metabolismo , Células Matadoras Naturais/imunologia , Receptores Imunológicos/metabolismo , Trofoblastos/imunologia , Decídua/citologia , Feminino , Antígenos HLA/metabolismo , Antígenos HLA-C/metabolismo , Antígenos HLA-G , Humanos , Placentação/imunologia , Gravidez , Antígenos HLA-ERESUMO
In this paper definitive evidence that the classical class I product, HLA-C, is expressed on the surface of normal trophoblast cells is provided. HLA-C transcripts were sequenced from cDNA isolated from first trimester trophoblast cells obtained by flow cytometric sorting. Both paternal and maternal alleles were transcribed. HLA-C proteins were demonstrated by biochemical analysis and found on the cell surface in association with beta(2)-microglobulin. Upregulation of cell surface HLA-C but not HLA-G expression after interferon (IFN)-gamma treatment was demonstrated by flow cytometric analysis. Immunohistology has confirmed HLA-C is expressed by all extravillous subpopulations in vivo. The question of whether trophoblast HLA-C molecules interact with decidual NK cells expressing killer Ig-like receptors (KIR) has also been addressed. Our results demonstrate that extravillous trophoblast expresses at least two HLA class I molecules, HLA-G and HLA-C on the cell surface.
Assuntos
Antígenos HLA-C/biossíntese , Trofoblastos/metabolismo , Adulto , Antígenos de Superfície/biossíntese , Antígenos de Superfície/genética , Coriocarcinoma/metabolismo , Testes Imunológicos de Citotoxicidade , Eletroforese em Gel de Poliacrilamida , Feminino , Citometria de Fluxo , Técnica Indireta de Fluorescência para Anticorpo , Antígenos HLA/biossíntese , Antígenos HLA-C/genética , Antígenos HLA-G , Antígenos de Histocompatibilidade Classe I/biossíntese , Humanos , Interferon gama/farmacologia , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Gravidez , RNA Mensageiro/biossíntese , Receptores Imunológicos/imunologia , Receptores Imunológicos/metabolismo , Receptores KIR , Receptores KIR2DL1 , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/metabolismo , Trofoblastos/efeitos dos fármacos , Células Tumorais Cultivadas , Regulação para Cima , Microglobulina beta-2/metabolismoRESUMO
This study has examined the expression of adhesion molecules by uterine-specific CD56bright CD16-CD3- large granular lymphocytes using flow cytometry and immunohistology. Data are compared with those obtained after IL-2 stimulation and with peripheral blood CD56+ cells. We have found that decidual CD56bright cells strongly express three fibronectin receptors, alpha 4 beta 1, alpha 5 beta 1, and alpha 4 beta 7. The HML-1 antigen is also expressed. The beta 2 integrins are also found on decidual CD56+ cells although, apart from CD11c, these are present at levels lower than those of corresponding cells in blood. After IL-2 stimulation there is a considerable increase in CD11a/CD18 expression on CD56bright cells. Both ICAM-1 and NCAM are found on CD56bright cells at higher levels compared to those of peripheral blood CD56+ cells. This pattern of expression of beta 1 and beta 2 integrins and members of the immunoglobulin superfamily must have important implications in the behaviour of decidual LGL. These adhesion molecules are also likely to be important in the recruitment and retention of CD56bright LGL in the decidual microenvironment particularly as the specialised decidual extracellular matrix and uterine LGL appear concomitantly at the time of implantation.
Assuntos
Antígenos CD/análise , Antígenos de Diferenciação de Linfócitos T/análise , Moléculas de Adesão Celular/análise , Integrinas/análise , Interleucina-2/farmacologia , Linfócitos/imunologia , Receptores de Fibronectina/análise , Complexo CD3/análise , Antígeno CD56 , Células Cultivadas/efeitos dos fármacos , Decídua/imunologia , Feminino , Citometria de Fluxo , Regulação da Expressão Gênica/imunologia , Humanos , Imuno-Histoquímica , Molécula 1 de Adesão Intercelular , Linfócitos/efeitos dos fármacos , Gravidez , Receptores de IgG/análiseRESUMO
During the process of implantation in humans, fetal trophoblast cells invade and migrate into the maternal decidua. During this migration, trophoblast cells destroy the wall of the maternal spiral arteries, converting them from muscular vessels into flaccid sinusoidal sacs. This vascular transformation is important to ensure an adequate blood supply to the feto-placental unit. Both cell-cell and cell-matrix interactions are important for trophoblast invasion of the decidual stroma and decidual spiral arteries. Cell-matrix adhesions are mediated by specific receptors, mostly belonging to the family of integrins. Signals transduced to the cells from the matrix via integrins could play a pivotal role in the control of cellular behaviour and gene expression, such as metalloproteinases that facilitate matrix degradation and tissue remodelling. This review focuses on the role of integrins and extracellular matrix in trophoblast cell migration, trophoblast invasion of the decidual spiral arteries and matrix degradation by trophoblast during implantation.
Assuntos
Implantação do Embrião/fisiologia , Placenta/fisiologia , Trofoblastos/fisiologia , Artérias , Decídua/irrigação sanguínea , Proteínas da Matriz Extracelular/fisiologia , Feminino , Humanos , Integrinas/fisiologia , GravidezRESUMO
At the time of implantation, the decidua is infiltrated by a unique population of NK cells with large granular lymphocyte morphology, which are thought to influence placental trophoblast invasion and differentiation. The mechanisms used by these cells to migrate within decidua are not known, but in other biological processes such as wound healing and tumor invasion cell-matrix interactions are important. These interactions are mediated by specific receptors, mostly belonging to the family of integrins. Decidual NK cells are observed to bind to type IV collagen and fibronectin, but not to laminin. Adhesion to collagen was inhibited with an anti-alpha 1 integrin subunit mAb, whereas adhesion to fibronectin was blocked with anti-alpha 4, -alpha 5, and -beta 1 integrin subunit mAbs. Binding of decidual NK cells to decidual stromal cells was partially blocked with mAbs to the alpha 4 and alpha 5 integrin subunits. These results provide insight into the possible mechanisms utilized by decidual NK cells for migration and retention within the pregnant uterine mucosa.
Assuntos
Adesão Celular/imunologia , Decídua/citologia , Matriz Extracelular/imunologia , Integrinas/fisiologia , Células Matadoras Naturais/fisiologia , Células Estromais/imunologia , Células 3T3 , Animais , Proteínas da Matriz Extracelular/imunologia , Feminino , Fibroblastos/imunologia , Humanos , Integrinas/imunologia , Camundongos , GravidezRESUMO
The process of placental implantation involves a series of transformations of trophoblast from a single polarized epithelial layer resting on a basement membrane (villous trophoblast), to cellular aggregates (trophoblast columns) which ultimately disperse to invade uterine decidua as individual cells (interstitial trophoblast). Such tissue re-modelling is associated with changes in the constituents of the extracellular matrix and in the expression of matrix receptors by the cells, the most relevant being the family of integrins which bind to laminin and fibronectin. In this study we show, by immunohistology and flow cytometry, a gradual loss of laminin receptors with the concomitant acquisition of fibronectin receptors as trophoblast is transformed from the villous phenotype, through the cell columns, into the extravillous population. The pattern of staining for the alpha 5, alpha 6, beta 1 and beta 4 subunits indicates that the integrins expressed by trophoblast are predominantly the alpha 5 beta 1 and the alpha 6 beta 4 heterodimers. We have also shown that isolated trophoblast cells assume a flattened, sessile phenotype when cultured on laminin but exhibit a more spreading, motile morphology when plated on fibronectin. In addition, numerous multinucleated giant cells are observed on a fibronectin substrate. Our data suggest that the relative expression of laminin and fibronectin receptors may determine the morphology and behaviour of trophoblast during the process of implantation.
Assuntos
Fibronectinas/metabolismo , Expressão Gênica , Integrinas/metabolismo , Laminina/metabolismo , Trofoblastos/fisiologia , Adesão Celular , Células Cultivadas , Fibronectinas/análise , Citometria de Fluxo , Imunofluorescência , Humanos , Imuno-Histoquímica , Integrinas/análise , Laminina/análise , Trofoblastos/química , Trofoblastos/citologiaRESUMO
At the time of implantation, the extracellular matrix proteins laminin and fibronectin are abundant in the decidua and are distributed pericellularly around each individual stromal cell. First trimester human trophoblast expresses both laminin and fibronectin receptors, specifically the alpha 1 beta 1, alpha 5 beta 1, alpha 6 beta 1 and alpha 6 beta 4 integrin heterodimers. In this study we have demonstrated that in-vitro adhesion of first trimester human trophoblast to purified extracellular matrix proteins and to purified decidual stromal cell monolayers can be inhibited by monoclonal antibodies directed against appropriate integrin subunits and by synthetic peptides containing an arginine-glycine-aspartic acid sequence. Monoclonal antibodies (mAbs) to the alpha 5 and beta 1 integrin subunits and a synthetic peptide significantly inhibited adhesion to fibronectin. Binding of trophoblast to laminin was blocked with mAbs to the alpha 6 and beta 1 but not alpha 1 and beta 4 integrin subunits. Similarly, integrin-mediated adhesion to monolayers of decidual stromal cells could be blocked with mAbs to the alpha 5, alpha 6, beta 1 and beta 4 integrin subunits. Integrin-mediated signal transduction in normal and malignant trophoblast was investigated by Western blotting. A 115 kDa protein was the major tyrosine phosphorylated protein detected in trophoblast after binding to laminin or fibronectin. The profile of tyrosine phosphorylated proteins differed for malignant trophoblast.
Assuntos
Fibronectinas/metabolismo , Integrinas/fisiologia , Laminina/metabolismo , Transdução de Sinais , Trofoblastos/metabolismo , Células 3T3 , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/farmacologia , Adesão Celular , Coriocarcinoma , Decídua/citologia , Feminino , Humanos , Integrinas/imunologia , Camundongos , Dados de Sequência Molecular , Peptídeos/farmacologia , Gravidez , Células Estromais/citologia , Células Tumorais CultivadasRESUMO
The main population of lymphocytes found in the human decidua during early pregnancy are NK-like cells with a distinctive phenotype, CD56bright CD16- CD3-. These cells are in close association with invading trophoblast that may be their in vivo target. We have examined three aspects of decidual NK function in vitro: cytotoxicity, proliferation, and cytokine production. The functional assays indicate uterine lymphocytes differ fundamentally from both PBL and even from classical circulating NK cells. Their role in the establishment of normal pregnancy remains unknown.
Assuntos
Citotoxicidade Imunológica , Decídua/imunologia , Células Matadoras Naturais/imunologia , Feminino , Humanos , GravidezRESUMO
Mice in which the gene that encodes the receptor (R) for leukemia inhibitory factor (LIF) has been deleted show abnormal growth and development of the placenta. This indicates that LIF plays an important role in placental development. The expression of LIF-R and LIF was examined in human trophoblast and decidua using in situ hybridization and immunocytochemistry. LIF-R mRNA and immunoreactivity was localized in villous and extravillous trophoblast throughout pregnancy, and in endothelial cells of the fetal villi. Strong expression of mRNA encoding LIF was detected in decidual leukocytes, which are abundant at the implantation site. Extravillous trophoblast, which invades the maternal decidua, therefore expresses LIF-R as it moves past decidual leukocytes, which express LIF mRNA. The effect of LIF on cultured human trophoblast was examined in vitro. Recombinant human LIF had no effect on [3H]thymidine incorporation by purified extravillous trophoblast, nor on expression of integrins alpha1, alpha5, or beta1 by isolated trophoblast. These results identify fetal endothelial cells and all cells of the trophoblast lineage as targets for the action of LIF in human placenta. Although its effects on trophoblast are not yet clear, LIF appears to mediate interactions between maternal decidual leukocytes and invading trophoblast. LIF may also play a critical role in controlling angiogenesis in the placental villi, since human fetal endothelial cells express LIF-R, and mice lacking a functional LIF receptor gene show altered vascular development in the placenta.