Assuntos
Európio/efeitos da radiação , Gadolínio/efeitos da radiação , Hólmio/efeitos da radiação , Lantânio/efeitos da radiação , Muramidase/efeitos da radiação , Neodímio/efeitos da radiação , Conformação Proteica , Análise Espectral , Aminoácidos/análise , Sítios de Ligação/efeitos da radiação , Luz , Fotoquímica , Efeitos da Radiação , Raios UltravioletaRESUMO
The S-peptide of the enzyme bovine pancreatic ribonuclease has been used as a model for covalent semisynthesis. Methods for side-chain protection, enzymatic cleavage of the peptide chain at the level of the single arginine-10 and for selective deprotection of the alpha-carboxyl function of this residue, have been examined. The partially protected [1-10] sequence has been coupled to a solid-phase generated [11-15] sequence attached to the polymer. After deblocking from the solid-support, the [1-15] semisynthetic peptide was complexed with native S-protein to give a complex with high biological activity.
Assuntos
Fragmentos de Peptídeos/síntese química , Ribonuclease Pancreático/síntese química , Sequência de Aminoácidos , Animais , Arginina , Bovinos , Indicadores e Reagentes , Ribonuclease Pancreático/metabolismo , TripsinaRESUMO
The peptide corresponding to the (66-104) sequence of horse heart cytochrome c and its carboxyamide analog, selectively modified at the critical Met80 residue, have been synthesized by stepwise solid-phase methods on PAM and BHA resins respectively. The correctness of the growing peptide chain as well as the homogeneity of the final products have been monitored by several analytical methods including quantitative Edman degradation. After HF cleavage both peptides were purified by semipreparative HPLC. The overall yields were 24% for the native (66-104) and 10% for the carboxyamide analog. The homogeneity of the purified synthetic peptides have been determined by different criteria including HPLC, amino acid composition, Edman degradation, electrophoresis, and tryptic peptide mapping. The synthetic fragments have been utilized for preliminary semisynthesis experiments with the native [Hse greater than 65] (1-65)H heme-sequence.