RESUMO
Objective: To compare the effects between hybrid surgery and transabdominal preperitoneal surgery in treatment of irreducible inguinal hernia. Methods: A total of 60 patients who underwent laparoscopic inguinal hernia repair between June 2011 and December 2017 were included in the study. Patients were divided into two group: hybrid surgery group (observation group, n=30) and transabdominal preperitoneal group (control group, n=30). The operation time, intraoperative bleeding, hospital stay, hospital cost and complications were analyzed. Results: The operative time of observation group and control group was 45 (35-65) minutes and 50(35-70) minutes, respectively. Intraoperative blood loss of two groups was 10(5-15) ml and 5(2-10) ml. The length of postoperative hospital stay was 2(1-4) days and 2(1-3) days in the two groups, respectively. And the hospitalization cost of two groups was 9 646 (9 066-11 560) yuan and 9 494(8 989-10 660) yuan, respectively. The intraoperative complications occurred in 4 cases in control group, including 1 case of vas deferens injury, 2 cases of spermatic vessel injury and 1 case of inferior epigastric artery injury. No intraoperative complications occurred in observation group. Perioperative complications in observation group and control group included dysuria (6.7% vs 10.0%), scrotum hematoma (3.4% vs 0%), wound pain (46.7% vs 6.7%) and fever (16.7% vs 20.0%). Twelve months of follow-up was completed in all the patients, and no recurrence or infections occurred in the two groups. The incidence of seroma in observation group and control group was 26.7%, 33.3%, respectively. One case of foreign body sensation and one case of chronic pain occurred in control group. The incidence of perioperative wound pain in patients undergoing hybrid surgery was higher than those undergoing transabdominal preperitoneal surgery (P<0.05), but no statistical differences were observed for other variables between the two groups (all P>0.05). Conclusion: Hybrid surgery is safe and feasible for the treatment of irreducible inguinal hernia. Though with a higher incidence of postoperative acute pain, it may have advantages of avoiding injuries of the vas deferens and spermatic vessels.
Assuntos
Hérnia Inguinal , Laparoscopia , Estudos de Casos e Controles , Hérnia Inguinal/cirurgia , Herniorrafia , Humanos , Masculino , Telas Cirúrgicas , Resultado do TratamentoRESUMO
OBJECTIVE: To explore the surgical techniques and the clinical efficacy of laparoscopic transabdominal preperitoneal repair (TAPP) for recurrent inguinal hernia. METHODS: Clinical data of 367 patients with recurrent inguinal hernia who underwent TAPP repair from Mar. 2009 to Mar. 2015 in Beijing Chao-Yang Hospital of Capital Medical University were analyzed retrospectively. RESULTS: Laparoscopic operations were completed successfully in 365 cases, however, 2 cases were converted to open surgery.The operation time was (55.7±19.3) min (30-100 min) and the hospital stay was (4.9±2.7) d (2-12 d). The incidences of postoperative pain, hydrocele, and urinary retention were 4.1%(15/367), 13.1%(48/367), and 1.3%(5/367) respectively.Other complications such as foreign body sensation, wound infection, and intestinal obstruction.All cases were followed up from 3 to 72 months ((36.5±14.7) months), 2 recurrent cases was observed and no mesh infection and long-term chronic pain were observed. CONCLUSIONS: Laparoscopic TAPP repair has advantages of minimal invasion and few complications, which is safe and effective for recurrent inguinal hernia.
Assuntos
Hérnia Inguinal/cirurgia , Laparoscopia/métodos , Telas Cirúrgicas , Dor Crônica , Herniorrafia , Humanos , Obstrução Intestinal , Tempo de Internação , Masculino , Dor Pós-Operatória , Estudos Retrospectivos , Procedimentos Cirúrgicos Operatórios/métodos , Hidrocele Testicular , Resultado do TratamentoRESUMO
Bacterial diversity and the major flora present on air-packaged broiler meat during storage at normal (4°C) and fluctuating storage temperatures (0-4°C and 4-10°C) were investigated using culture-dependent and culture-independent approaches. Culture-dependent analysis revealed that the growth of microflora was retarded when broiler meat was stored at lower temperatures (0-4°C). Denaturing gradient gel electrophoresis profiles showed that Staphylococcus spp., Pseudomonas spp., Acinetobacter spp., Carnobacterium spp., Aeromonas spp., and Weissella spp. were the dominant bacteria throughout all storage conditions. Enterobacteriaceae only appeared in samples subjected to storage with high temperature abuse, whereas Shewanella spp. and Psychrobacter spp. were only detected in samples stored below 4°C. Our results provide evidence that, compared with storage at a standard fixed temperature (4°C), fluctuations in temperatures induce a more complex bacterial diversity in the air-packaged broiler.
Assuntos
Bactérias/crescimento & desenvolvimento , Embalagem de Alimentos , Conservação de Alimentos , Carne/microbiologia , Animais , Bactérias/classificação , Bactérias/isolamento & purificação , Galinhas , Análise por Conglomerados , Contagem de Colônia Microbiana/veterinária , DNA Bacteriano/análise , Eletroforese em Gel de Gradiente Desnaturante/veterinária , Carne/normas , Filogenia , Reação em Cadeia da Polimerase/veterinária , RNA Ribossômico 16S/análise , TemperaturaRESUMO
A one-dimensional diatomic meta-chain with equal-size holes and different-length slits is designed. Broadband coherent magnetic plasmon waves (MPW) are formed in such a system, excited by both the electric resonance in the slits and the magnetic resonance in the holes in a wide range of incidence angles (0°-40°) and broad frequency bands (200-230 THz). The dispersion properties of the MPW measured in our experiments agree with the theoretical calculation based on the Lagrange model. The coherent MPWs reported in this paper may have applications in subwavelength integrated nanocircuits.
Assuntos
Modelos Teóricos , Ressonância de Plasmônio de Superfície/instrumentação , Ressonância de Plasmônio de Superfície/métodos , Simulação por Computador , Desenho Assistido por Computador , Campos Eletromagnéticos , Desenho de Equipamento , Análise de Falha de EquipamentoRESUMO
Magnetic resonance coupling between connected split ring resonators (SRRs) and magnetic plasmon (MP) excitations in the connected SRR chains were theoretically studied. By changing the connection configuration, two different coupling behaviors were observed, and therefore two kinds of MP bands were formed in the connected ring chains accordingly. From the extracted dispersion properties of MPs, forward and backward characteristics of the guided waves are well exhibited corresponding to the homo- and hetero-connected chains. Notably, thanks to the conductive coupling the revealed MP waves both have wide bandwidth even starting from the zero frequency. These results are suggested to provide instructions to build new kinds of subwavelength waveguides.
Assuntos
Ressonância de Plasmônio de Superfície/instrumentação , Simulação por Computador , Desenho de Equipamento , Magnetismo , Modelos Estatísticos , Óptica e Fotônica/instrumentaçãoRESUMO
The electronic and optical properties of codoped double-walled carbon nanotubes (DWNTs), in which nucleophilic atoms (potassium) are adsorbed outside the outer tube and electrophilic molecules (NO(2)) are adsorbed inside the inner tube, are investigated by density functional theory. It is found that the inner core tube is p-type doped and the outer shell tube is n-type doped, forming a radial p-n junction of DWNTs. A type-II band energy alignment is formed at the interface of two constituting walls of codoped DWNTs. Moreover, optical calculations show that the band edge absorption is zero for pristine DWNTs, while it is pronounced for the codoped DWNTs. This absorption of codoped DWNTs leads to charge separation in the interface of two walls of DWNTs with holes located on core tube while electrons located on shell one. The properties of DWNT coaxial nanocables demonstrated here can find future applications in electronic and optoelectronic devices.
RESUMO
Objective: To analyze and summarize the method and effect of cervical esophagus perforation. Methods: A total of 29 cervical esophageal perforation patients caused by foreign body were retrospectively analyzed from January 2012 to June 2018 in Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Nanchang University. Results: Among the 29 cervical esophageal perforation patients, 28 patients were extracted by lateral neck incision, 1 patient with carotid artery hemorrhea was rescued by repairing the fistula between carotid artery and esophage. All patients recovered and successfully discharged. There were no severe complications among all patients. Conclusions: The cervical CT scan is important for diagnosing a cervical esophageal perforation. Enhancement CT scan is necessay for a patient with haematemesis. Lateral neck incision would be first choice for patients with big foreign bodies. Inflammatory reaction and finger palpation are helpful for locating foreign bodies.
Assuntos
Perfuração Esofágica/diagnóstico , Perfuração Esofágica/cirurgia , Corpos Estranhos , Adulto , Perfuração Esofágica/diagnóstico por imagem , Perfuração Esofágica/etiologia , Feminino , Corpos Estranhos/complicações , Humanos , Masculino , Pescoço , Estudos Retrospectivos , Tomografia Computadorizada por Raios XRESUMO
In order to figure out the effect of ultrasound treatments (UT) on the tenderizing pathway of goose meat, breast muscles of 32 Eastern Zhejiang White Geese were treated with different ultrasound powers (the control, 300 and 600 W) at 40 kHz for 30 min. Shear force, cooking loss, myofibrillar fraction index (MFI), particle size, morphological analysis of actin filaments and the levels of F-actin and G-actin were investigated during 168 h storage. Results showed that 600 W group had the lowest shear force and cooking loss at 24, 48, 96 and 168 h among treatments, while 300 W UT decreased shear force and cooking loss compared to the control. UT increased MFI and induced myofibrillar small particles (D3,2) by disrupting actin filaments for myofibril and transforming of F-actin to G-actin compared to the control. We concluded that UT tenderized goose meat by fragmenting actin filaments and myofibrillar fraction.
Assuntos
Armazenamento de Alimentos , Carne/análise , Músculos Peitorais/fisiologia , Ultrassonografia , Actinas/metabolismo , Animais , Proteínas Aviárias/metabolismo , Culinária , Gansos/fisiologia , Miofibrilas/fisiologia , Resistência ao CisalhamentoRESUMO
To evaluate the effect of structural change on the digestibility of sarcoplasmic proteins in Nanjing dry-cured duck during processing, carbonyl content, sulfhydryl (SH) group, disulfide (S-S) group, surface hydrophobicity, particle size, secondary structures, and in vitro digestibility were determined. During processing, carbonyl content increased; SH groups turned into S-S groups; α-helix turned into ß-sheet. From marinating to early dry-ripening stage, surface hydrophobicity increased but particle size decreased, whereas these had opposite tendencies at the late dry-ripening stage. The in vitro digestibility of pepsin decreased at drying-curing and drying-ripening stages, whereas the one of pancreatic proteases kept stable until late drying-ripening stage. We concluded that salting, drying, and protein oxidation caused the denaturation and aggregation during processing. The oxidation and aggregation of sarcoplasmic proteins of Nanjing dry-cured duck resulted in a loss of nutritional quality during drying-ripening stage.
Assuntos
Digestão , Manipulação de Alimentos/métodos , Proteínas Musculares/análise , Proteínas de Aves Domésticas/análise , Animais , PatosRESUMO
Vinasse-cured duck was processed by boiling, salting, and vinasse-dry-curing or vinasse-wet-curing. Volatile compounds, moisture contents, reducing sugars, and thiobarbituric acid reactive substances (TBARS) values during processing were determined. Reducing sugars decreased during boiling and increased after vinasse-curing. TBARS values increased throughout the processing. The volatiles of ducks during processing and vinasse-curing agents (dry vinasse and vinasse sauce) were 125 compounds. The first principal component (PC1) belonged to the flavor of cooked duck, whereas the second principal component (PC2) represented the flavor of yellow rice wine. The 66.49 and 29.61% of typical aroma of vinasse-dry-cured product were from dry vinasse and lipid oxidation, respectively, whereas the 75.58 and 21.89% of aroma of vinasse-wet-cured product were from vinasse sauce and lipid oxidation, respectively. Lipid oxidation products increased after boiling, whereas salting promoted their release. Compared with vinasse-wet-cured duck, vinasse-dry-cured product presented richer aroma of lipid oxidation and weaker flavor of vinasse-curing agents. Besides, protein denaturation which was caused by ethanol could influence the retention and release of flavor. In conclusion, two kinds of vinasse-curing methods influenced the characteristic volatiles of products and their generation during processing.
Assuntos
Manipulação de Alimentos/métodos , Carne/análise , Açúcares/análise , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Compostos Orgânicos Voláteis/análise , Animais , PatosRESUMO
The prevalence of child and adolescent growth and mental-behavior related diseases are increasing, and the pathogenesis are complex. Twins are excellent natural resources for complex chronic diseases research as they share the maternal intrauterine environment, born at the same time and share the same family environment in early years, which could benefit the adjust ment of confounding factors, such as age, genetic factors and early family environmental factors. Birth cohort with twin families involved could be more effective in exploring the genetic and environmental factors for complex chronic diseases at the very beginning of life. This paper summarizes the objective, content, progress, strengths and potential problems of Wuhan Twin Birth Cohort, with emphasis on the overall design and progress of the study.
Assuntos
Povo Asiático , Doenças em Gêmeos , Estudos em Gêmeos como Assunto , Gêmeos , Adolescente , Peso ao Nascer , Criança , China , Estudos de Coortes , Doenças em Gêmeos/etnologia , Doenças em Gêmeos/genética , Monitoramento Epidemiológico , Feminino , Humanos , MasculinoRESUMO
In order to clarify the influence of curing agents on the flavor of duck, the effect of pH on the surface hydrophobicity, secondary structures, and adsorption capacity of myofibrillar proteins to alcohols, aldehydes, ketones, and esters was assessed using Raman spectroscopy, gas chromatography-mass spectrometer, and other methodologies. The hydrophobicity decreased as pH increased from 5.0 to 8.0; ß-turn turned into α-helix and random coil as pH increased from 5.0 to 7.0, while α-helix and random coil turned into ß-sheet and ß-turn as pH increased from 7.0 to 8.0. With the increase of pH, the decreased adsorbing of alcohols could depend on hydrogen bonds. As pH increased from 5.0 to 8.0, the increase of aldehydes and esters was attributed to the unfolding of myofibrillar proteins and decreased hydrophobicity. The decreased adsorbing of ketones was due to the decreased hydrophobicity as pH increased from 5.0 to 8.0. The present work provided information about the correlation between structure and adsorption capacity of myofibrillar proteins to flavor compounds.
Assuntos
Patos , Carne/análise , Proteínas Musculares/química , Estrutura Secundária de Proteína/efeitos dos fármacos , Compostos Orgânicos Voláteis/química , Adsorção , Animais , Manipulação de Alimentos/métodos , Concentração de Íons de HidrogênioRESUMO
BACKGROUND: Anti-angiogenesis therapy has showed a promising future in tumour treatment. More and more evidence suggest that endoglin is a powerful marker of angiogenesis in solid malignancies, including liver cancer. AIM: To explore whether a plasmid DNA encoding the porcine endoglin has the ability of breaking immune tolerance against endoglin-related tumour angiogenesis in mice. METHODS: A eukaryotic plasmid encoding the extracellular domain of porcine endoglin was constructed, and then used it as a xenogeneic DNA vaccine. Hepa1-6 and H22 hepatoma models were established to observe the anti-tumour activities. Western blot, enzyme-linked immunoadsorbent assay and enzyme-linked immunospot assay were used to determine the antibody characters. Immunohistochemistry and alginate-encapsulated tumour cell assay were used to observe the anti-angiogenesis effects. RESULTS: Immunotherapy with recombinant plasmid encoding extracellular domain of porcine endoglin was effective at both protective and therapeutic anti-tumour immunity in two hepatoma models. Autoantibodies against murine endoglin were identified. IgG1 and IgG2b were the major subclasses in response to recombinant plasmid encoding extracellular domain of porcine endoglin vaccination. Anti-endoglin antibody-producing B cells were significantly increased in the spleens of mice immunised with recombinant plasmid encoding extracellular domain of porcine endoglin. In addition, mouse self-immunoglobulins were found deposited on the blood vessels of recombinant plasmid encoding extracellular domain of porcine endoglin-immunised tumour tissues. The similar anti-tumour activity was induced by the adoptive transfer of the purified immunoglobulins from the sera of mice immunised with recombinant plasmid encoding extracellular domain of porcine endoglin. Furthermore, angiogenesis was apparently inhibited within the tumour tissues from the recombinant plasmid encoding extracellular domain of porcine endoglin-immunised mice, and the vascularisation of alginate balls was also reduced in recombinant plasmid encoding extracellular domain of porcine endoglin-immunised mice. Most importantly, recombinant plasmid encoding extracellular domain of porcine endoglin could really induce cytotoxic T lymphocyte-mediated cytotoxicity and inhibit cell proliferation against endothelial cells. In addition, both CD4+ and CD8+ T lymphocytes took part in the function of inhibiting tumour growth and were synergistically responsible for induction of the anti-tumour activities. CONCLUSIONS: This approach may provide an alternative strategy for liver cancer immunotherapy.
Assuntos
Vacinas Anticâncer/farmacologia , Carcinoma Hepatocelular/terapia , Neoplasias Hepáticas Experimentais/terapia , Neovascularização Patológica/terapia , Plasmídeos/farmacologia , Vacinas de DNA/farmacologia , Transferência Adotiva , Animais , Apoptose/efeitos dos fármacos , Apoptose/imunologia , Autoanticorpos/efeitos dos fármacos , Autoanticorpos/imunologia , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/imunologia , Western Blotting , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/efeitos dos fármacos , Linfócitos T CD8-Positivos/imunologia , Vacinas Anticâncer/toxicidade , Carcinoma Hepatocelular/imunologia , Proliferação de Células/efeitos dos fármacos , Citotoxicidade Imunológica/efeitos dos fármacos , Citotoxicidade Imunológica/imunologia , Endoglina , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/imunologia , Ensaio de Imunoadsorção Enzimática , Tolerância Imunológica/efeitos dos fármacos , Tolerância Imunológica/imunologia , Imunoglobulina G/efeitos dos fármacos , Imunoglobulina G/imunologia , Imunoterapia , Peptídeos e Proteínas de Sinalização Intracelular/efeitos dos fármacos , Peptídeos e Proteínas de Sinalização Intracelular/imunologia , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Fígado/irrigação sanguínea , Neoplasias Hepáticas Experimentais/imunologia , Camundongos , Neovascularização Patológica/imunologia , Plasmídeos/toxicidade , Subpopulações de Linfócitos T/efeitos dos fármacos , Subpopulações de Linfócitos T/imunologia , Vacinas de DNA/toxicidade , Vacinas Sintéticas/farmacologia , Vacinas Sintéticas/toxicidadeRESUMO
Twenty-four Eastern Zhejiang White Geese were slaughtered, dry-cured by 2 different kinds of salt contents (12 geese with 4% low salt level [LS]; 12 geese with 8% high salt level [HS]) for one d, marinated in brine for one d, and air dry-ripened at 16 °C for 7 d. The effect of dry-curing salt contents on the changes in myofibril proteins, potential proteolysis activities, and total free amino acid (TFAA) in dry-cured goose was investigated. Compared to the raw, cathepsin B+L and calpains activities decreased at the end of dry-curing and the third d of dry-ripening. At the final products, the activities of cathepsin B+L and calpains were about half of those in raw meat. There was no difference in proteolysis activities except for the end of dry-curing (P < 0.05) and the 3 d of dry-ripening (P < 0.05) for cathepsin B+L, and the end of dry-curing (P < 0.05) for calpains (P < 0.05) between groups. Myosin light chain (MLC) and troponin-I were cleaved. Compared to the raw, TFAA increased by 36.64 and 31.82% in the final products for HS (P < 0.001) and LS (P < 0.01), respectively. The increase of TFAA could be attributed to the proteolysis of myofibril proteins and retained proteolysis activities. No significant difference on TFAA and MLC and troponin-I bands was observed between groups in final products. This means that different proteolysis activities during processing did not cause the difference in quality of final products between groups, and that 4% low salt can be used in future applications.
Assuntos
Manipulação de Alimentos/métodos , Carne/análise , Cloreto de Sódio/química , Aminoácidos/análise , Animais , Gansos , Proteínas Musculares/análise , Miofibrilas/química , Peptídeo Hidrolases/análiseRESUMO
We have noted a striking similarity between the sequences of proteins in a novel family of lipases we recently reported [Upton, C. and Buckley, J. T. (1995) Trends Biol. Sci. 20, 178-9] and more than 120 sequences from the database of Expressed Sequence Tags (dbEST) which correspond to at least 30 unique genes from arabidopsis, rice and maize. A cDNA (Arab-1) corresponding to one of these sequences was isolated, sequenced and translated. There was significant similarity to sequences in the new lipase family over the entire open reading frame of Arab-1 and when expressed in E. coli, the gene product was lipolytic. Arab-1 and genes for some of the other plant proteins appear to be differentially expressed. They may play a role in the regulation of lipid metabolism during plant development.
Assuntos
Lipase/genética , Lipólise/genética , Plantas/enzimologia , Sequência de Aminoácidos , Arabidopsis/enzimologia , Arabidopsis/genética , Sequência de Bases , Butiratos/metabolismo , DNA Complementar/genética , Expressão Gênica , Dados de Sequência Molecular , Oryza/enzimologia , Oryza/genética , Plantas/genética , RNA Mensageiro/análise , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Transcrição Gênica , Zea mays/enzimologia , Zea mays/genéticaRESUMO
We have identified a human gene encoding a protein with 48% amino acid identity to the vaccinia virus (VV) K4L gene product. Both contain motifs characteristic of the phospholipase D (PLD) protein superfamily. These proteins are also related to vaccinia virus p37, encoded by the F13L gene, which is required for envelopment and spread of the virus. The similarity to phospholipase D provides insight into the mechanisms and evolution of these processes.
Assuntos
Fosfolipase D/genética , Vaccinia virus/genética , Proteínas do Envelope Viral/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Complementar , Desoxirribonuclease HindIII , Humanos , Proteínas de Membrana/genética , Dados de Sequência Molecular , Fases de Leitura Aberta , Homologia de Sequência de AminoácidosRESUMO
Viruses and the immune system have been competitors throughout their co-evolution. It is therefore not surprising that the viruses in circulation today possess a variety of strategies to counteract those aspects of the immune system that are involved in virus clearance. Examination of these virus encoded functions provides an important view of immune function and an appreciation of the complexity of the virus-host interaction. It is clear that viruses, seeking to subvert the immune system, have become adept in blocking the communication channels of the immune system. There are numerous examples of viral proteins that target the cytokine networks, disrupting the processes by which the delicately balanced immune system is regulated. This review focuses on the gene products of poxviruses, adenoviruses and herpesviruses that function primarily as immune-modulators.
Assuntos
Citocinas/fisiologia , Vírus/imunologia , Adenoviridae/genética , Adenoviridae/imunologia , Animais , Humanos , Poxviridae/genética , Poxviridae/imunologia , Transdução de Sinais/imunologia , Simplexvirus/genética , Simplexvirus/imunologiaRESUMO
In the present paper is stated the accomplishment of the studies of 17 populations of cultivated Fagopyrum tataricum (L.) Gaertn. from the counties of Yuexi and Ganluo in North Liangshan Autonomous Prefecture of Yi Nationality, Sichuan Province, China by means of starch gel electrophoresis and cluster analysis of agrobiology features. Seven enzymes, and 15 loci were assessed. The result shows that the genetic diversity of F. tataricum within population is higher than in the South Prefecture and in species F. esculentum from other areas. The A, P, Ho and He are 1.9, 52.1%, 0.190 and 0.262 respectively. The FST is 0.199 which is close to the average level of wild plants. A conservation strategy of the genetic resource of F. tataricum is suggested. Two populations of local variety "Youchiqiao" have the richest genetic diversity, P = 60.0%, Ho = 0.260 and 0.301 and they should be protected particularly. Two populations of local variety "Xiaomiqiao" with higher genetic diversity, as well as three populations of local varieties of "Qiukuqiao", "Equkuqiao" and "Geluokuqiao" with special genetic position should be protected too.