RESUMO
A yellowish-pigmented bacterial strain, designated as MQ-18T, was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18T showed high similarities to Piscinibacter defluvii SH-1T (99.7â%) and Piscinibacter aquaticus IMCC1728T (98.4â%), thereby suggesting that it belongs to the genus Piscinibacter. The DNA-DNA relatedness values of this strain to strains SH-1T and IMCC1728T were only 35.4 and 33.3â%, respectively. Cells of MQ-18T were Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25-37 °C (optimum: 30 °C) in the presence of 0-3.0â% (w/v) NaCl (optimum, 0â% NaCl) and at pH 5.0-8.0 (pH 7.0). The predominant fatty acids were C12â:â0 (5.5â%), C16â:â0 (33.7â%), summed feature 3 (C16â:â1ω7c and/or C16â:â1ω6c; 38.5â%), and summed feature 4 (anteiso-C17â:â1 B and/or iso C17â:â1 I; 11.6â%). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18T is considered to represent a novel species of the genus Piscinibacter, for which the name Piscinibacter caeni sp. nov. is proposed. The type strain is MQ-18T (CCTCC AB 2017223T=JCM 32138T).
Assuntos
Burkholderiales/classificação , Filogenia , Esgotos/microbiologia , Bactérias/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , Burkholderiales/genética , Burkholderiales/isolamento & purificação , Cadaverina/química , China , DNA Bacteriano/genética , Indústria Farmacêutica , Ácidos Graxos/química , Resíduos Industriais , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , Putrescina/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
Bioaugmented biotrickling filter (BTF) seeded with Piscinibacter caeni MQ-18, Pseudomonas oleovorans DT4, and activated sludge was established to investigate the treatment performance and biodegradation kinetics of the gaseous mixtures of tetrahydrofuran (THF) and methyl tert-butyl ether (MTBE). Experimental results showed an enhanced startup performance with a startup period of 9 d in bioaugmented BTF (25 d in control BTF seeded with activated sludge). The interaction parameter I2,1 of control (7.462) and bioaugmented BTF (3.267) obtained by the elimination capacity-sum kinetics with interaction parameter (EC-SKIP) model indicated that THF has a stronger inhibition of MTBE biodegradation in the control BTF than in the bioaugmented BTF. Similarly, the self-inhibition EC-SKIP model quantified the positive effects of MTBE on THF biodegradation, as well as the negative effects of THF on MTBE biodegradation and the self-inhibition of MTBE and THF. Metabolic intermediate analysis, real-time quantitative polymerase chain reaction, biofilm-biomass determination, and high-throughput sequencing revealed the possible mechanism of the enhanced treatment performance and biodegradation interactions of MTBE and THF.
Assuntos
Éteres Metílicos , Pseudomonas oleovorans , Biodegradação Ambiental , Burkholderiales , Furanos , Éteres Metílicos/análiseRESUMO
The performance and microbial communities of methyl tert-butyl ether (MTBE) treatment using a biotrickling filter (BTF) that was inoculated with activated sewage sludge were investigated. The BTF successfully started up within 23 days when the inlet concentration of MTBE was 100 mg·m-3 and empty bed retention time was 60 s, with 70% removal efficiency (RE). Under steady-state conditions, an elimination capacity (EC) and a mineralization ratio of 13.47 g·(m3·h)-1 and 68% were achieved, respectively. The ECmax was 21.03 g·(m3·h)-1 according to the Haldane model, and a KS of 0.16 g·m-3 and KI of 0.99 g·m-3 were obtained. High-throughput sequencing was used to identify the community structure of the mixed microbial consortium in the BTF. The results indicated that Methylibium sp. (11.33%) and Blastocatella sp. (9.95%) were the dominant bacteria.