RESUMO
The analysis of temporal and spatial patterns of gene expression is critically important for many kinds of developmental studies, including the construction of gene regulatory networks. Recently, multiplex, fluorescent, whole mount in situ hybridization (multiplex F-WMISH), applied in combination with confocal microscopy, has emerged as the method of choice for high-resolution, three-dimensional (3D) mapping of gene expression patterns in developing tissues. We have developed an image analysis tool, GeneExpressMap (GEM), that facilitates the rapid, 3D analysis of multiplex F-WMISH data at single-cell resolution. GEM assigns F-WMISH signal to individual cells based upon the proximity of cytoplasmic hybridization signal to cell nuclei. Here, we describe the features of GEM and, as a test of its utility, we use GEM to analyze patterns of regulatory gene expression in the non-skeletogenic mesoderm of the early sea urchin embryo. GEM greatly extends the power of multiplex F-WMISH for analyzing patterns of gene expression and is a valuable tool for gene network analysis and many other kinds of developmental studies.
Assuntos
Perfilação da Expressão Gênica/métodos , Imageamento Tridimensional/métodos , Ouriços-do-Mar/genética , Animais , Biomarcadores/metabolismo , Núcleo Celular/genética , Bases de Dados Genéticas , Embrião não Mamífero/citologia , Embrião não Mamífero/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Hibridização In Situ , Reprodutibilidade dos Testes , Ouriços-do-Mar/citologiaRESUMO
The segregation of lateral geniculate nucleus (LGN) axons into ocular dominance columns is believed to involve a prolonged, activity-dependent sorting process. However, visualization of early postnatal ferret LGN axons by direct LGN tracer injections revealed segregated ocular dominance columns <7 days after innervation of layer 4. These early columns were unaffected by experimentally induced imbalances in retinal activity, implying that different mechanisms govern initial column formation and their modification during the subsequent critical period. Instead of activity-dependent plasticity, we propose that ocular dominance column formation relies on the targeting of distinct axonal populations to defined locales in cortical layer 4.
Assuntos
Axônios/fisiologia , Corpos Geniculados/fisiologia , Córtex Visual/crescimento & desenvolvimento , Vias Visuais/fisiologia , Percepção Visual , Animais , Animais Recém-Nascidos , Feminino , Furões , Corpos Geniculados/citologia , Masculino , Neurônios Aferentes/fisiologia , Estimulação Luminosa , Retina/fisiologia , Privação Sensorial , Visão Ocular , Córtex Visual/citologia , Córtex Visual/fisiologia , Vias Visuais/crescimento & desenvolvimentoRESUMO
This article assesses the status of scientific instruments in major research universities and identifies factors that facilitate or impede their development, acquisition, use, and maintenance. Sixteen universities, six national and government laboratories, and nine commercial laboratories were visited; over 700 individuals were interviewed. Data on instrument acquisition and age were collected. Instrumentation was examined in physics, chemistry, biological sciences, earth sciences, and electrical engineering. The study found that the quality of university instrumentation has seriously deteriorated, due principally to a relative decrease in instrumentation funding, inflexibility within the project grant system, and insufficient support for maintenance.
Assuntos
Equipamentos e Provisões/economia , Projetos de Pesquisa , Universidades , Custos e Análise de Custo , Apoio à Pesquisa como AssuntoRESUMO
The initial establishment of ocular dominance columns in visual cortex is believed to involve the segregation of overlapping geniculocortical axons into eye-specific patches based on patterns of correlated activity. However, we found that total removal of retinal influence early in visual development did not prevent segregation of geniculocortical axons into alternating stripes with periodicity normal for ocular dominance columns. Because the patterning of geniculocortical afferents resists this dramatic change in the level, source and pattern of spontaneous activity, we propose that formation of ocular dominance columns relies on molecular cues present on thalamic axons, cortical cells or both.
Assuntos
Retina/fisiologia , Privação Sensorial/fisiologia , Visão Ocular/fisiologia , Córtex Visual/crescimento & desenvolvimento , Córtex Visual/fisiologia , Vias Visuais/crescimento & desenvolvimento , Envelhecimento , Animais , Animais Recém-Nascidos , Axônios/fisiologia , Biotina/análogos & derivados , Biotina/metabolismo , Dextranos/metabolismo , Feminino , Furões , Corantes Fluorescentes/metabolismo , Corpos Geniculados/citologia , Corpos Geniculados/crescimento & desenvolvimento , Corpos Geniculados/fisiologia , Masculino , Microesferas , Neurônios Aferentes/fisiologia , Retina/cirurgia , Fatores de Tempo , Córtex Visual/citologia , Córtex Visual/efeitos dos fármacos , Vias Visuais/fisiologia , Percepção Visual/fisiologiaRESUMO
To continue the systematic examination of the physical and genetic organization of an entire Saccharomyces cerevisiae chromosome, the DNA from the CEN1-ADE1-CDC15 region from chromosome I was isolated and characterized. Starting with the previously cloned ADE1 gene (J. C. Crowley and D. B. Kaback, J. Bacteriol. 159:413-417, 1984), a series of recombinant lambda bacteriophages containing 82 kilobases of contiguous DNA from chromosome I were obtained by overlap hybridization. The cloned sequences were mapped with restriction endonucleases and oriented with respect to the genetic map by determining the physical positions of the CDC15 gene and the centromeric DNA (CEN1). The CDC15 gene was located by isolating plasmids from a YCp50 S. cerevisiae genomic library that complemented the cdc15-1 mutation. S. cerevisiae sequences from these plasmids were found to be represented among those already obtained by overlap hybridization. The cdc15-1-complementing plasmids all shared only one intact transcribed region that was shown to contain the bona fide CDC15 gene by in vitro gene disruption and one-step replacement to delete the chromosomal copy of this gene. This deletion produced a recessive lethal phenotype that was also recessive to cdc15-1. CEN1 was located by finding a sequence from the appropriate part of the cloned region that stabilized the inheritance of autonomously replicating S. cerevisiae plasmid vectors. Finally, RNA blot hybridization and electron microscopy of R-loop-containing DNA were used to map transcribed regions in the 23 kilobases of DNA that went from CEN1 to CDC15. In addition to the transcribed regions corresponding to the ADE1 and ADC15 genes, this DNA contained five regions that gave rise to polyadenylated RNA, at least two regions complementary to 4S RNA species, and a Ty1 transposable element. Notably, a higher than average proportion of the DNA examined was transcribed into RNA.
Assuntos
Cromossomos/fisiologia , Clonagem Molecular , DNA Fúngico/genética , Genes Fúngicos , Saccharomyces cerevisiae/genética , Centrômero/fisiologia , Enzimas de Restrição do DNA , DNA Recombinante/metabolismo , Genótipo , Hibridização de Ácido Nucleico , Plasmídeos , Transcrição GênicaRESUMO
Five experiments were performed to evaluate the effects of dexamethasone (DEX), gender, and testosterone on plasma leptin concentrations in horses. In experiment 1, plasma leptin, insulin, glucose, and IGF-1 concentrations were increased (P < 0.01) in stallions following five daily injections of DEX (125 microg/kg BW). In experiment 2, leptin concentrations increased (P < 0.01) in mares, geldings, and stallions following a single injection of DEX, and the response was greater (P < 0.01) in mares and geldings than in stallions. The gender effect was confounded by differences in body condition scores and diet; however, based on stepwise regression analysis, both BCS and gender were significant sources of variation in the best fit model for pre-DEX leptin concentrations (R(2) = 0.65) and for maximum leptin response to DEX (R(2) = 0.75). In experiment 3, in which mares and stallions were pair-matched based on age and body condition and fed similar diets, mares again had higher (P < 0.01) leptin concentrations than stallions after DEX treatment as used in experiment 2. In experiment 4, there was no difference (P > 0.1) in plasma leptin response in mares following four single-injection doses of DEX from 15.6 to 125 microg/kg BW. In experiment 5, treatment of mares with testosterone propionate every other day for 5 days did not alter (P > 0.1) plasma leptin concentrations or the leptin response to DEX. In conclusion, multiple injections of DEX increase leptin concentrations in stallions, as does a single injection in mares (as low as 15.6 microg/kg BW), geldings and stallions. The greater leptin levels observed in mares and geldings relative to stallions were due partially to their greater body condition and partially to the presence of hyperleptinemic individuals; however, even after accounting for body condition and diet, mares still had greater leptin concentrations than stallions after DEX administration. Elevation of testosterone levels in mares for approximately 10 days did not alter leptin concentrations in mares.
Assuntos
Dexametasona/farmacologia , Glucocorticoides/farmacologia , Cavalos/fisiologia , Leptina/metabolismo , Propionato de Testosterona/farmacologia , Tecido Adiposo/fisiologia , Animais , Peso Corporal/fisiologia , Feminino , Cavalos/sangue , Leptina/sangue , Masculino , Fatores SexuaisRESUMO
Listeria monocytogenes is an intracellular bacterial pathogen. A single gene product, listeriolysin (LLO), is critical for the induction of protective immunity. We now show that listeria that produce functional LLO augment Ia expression by macrophages and are better presented to a Th1, CD4+ anti-listeria T cell line. We used two genetically engineered strains of listeria which differed only in their ability (Ly+) or inability (Ly-) to produce functional LLO. Ia-negative murine macrophages ingested either Ly+ or Ly-, and then were stimulated by interferon-gamma (IFN-gamma). Increasing numbers of live Ly+, but not Ly-, augmented IFN-gamma-induced Ia expression. Ly+ by itself did not induce Ia expression. Heat-killed Ly+ and Ly- did not augment IFN-gamma-induced Ia expression. The abundance of Ia on the macrophage cell surface is one major determinant of antigen presentation to CD4+ T cells. Consistent with their ability to augment la expression, Ly+ were better presented than Ly- to a CD4+, Th1, anti-listeria T cell line. When macrophages and T cells were from different inbred mouse strains, antigen presentation required identity at the class II region of the MHC gene complex. This indicated that antigen presentation occurred via Ia molecules. The increased ability of macrophages to present Ly+ is a product of the macrophage-listeria interaction, not a property of the T cell tine 86. If Ia-negative macrophages ingested Listeria and were then stimulated by IFN-gamma, Ly+ was presented more efficiently than Ly-. On the other hand, if Ia-positive macrophages ingested Listeria, then Ly+ and Ly- were presented equally well to T cells. Altogether our data is consistent with the hypothesis that macrophages interact differently with Ly+, and that this contributes to the ability of only live Ly+ to induce protective immunity.
Assuntos
Apresentação de Antígeno , Toxinas Bacterianas , Proteínas de Choque Térmico/fisiologia , Proteínas Hemolisinas/fisiologia , Antígenos de Histocompatibilidade Classe II/análise , Listeria monocytogenes/imunologia , Animais , Linfócitos T CD4-Positivos/imunologia , Linhagem Celular , Feminino , Interferon gama/farmacologia , Camundongos , Camundongos Endogâmicos C3HRESUMO
Only Listeria monocytogenes that produce listeriolysin O (LLO) elicit protective immunity. Given the importance of tumor necrosis factor alpha (TNF-alpha) in anti-Listeria immunity, we have investigated TNF-alpha production by macrophages after they ingested live LLO-producing compared to LLO-non-producing bacteria. We used two genetically engineered strains of Listeria that differed only in their ability (Ly+) or inability (Ly-) to produce LLO. Ly+ and Ly- caused the same kinetics of increased mRNA abundance for TNF-alpha during the first 90 min after phagocytosis. However, only Ly+ caused sustained transcription of TNF-alpha mRNA, and this may account for the increased release of TNF-alpha. The transcriptional inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) prevented the sustained abundance of cytokine mRNA 20 h after ingestion of Ly+. In addition, nuclear run-on assays indicated sustained transcription of TNF-alpha genes only after ingestion of Ly+. LLO itself was not responsible for the ability of Ly+ to stimulate the sustained transcription of the TNF-alpha genes. Instead, LLO may allow Listeria to survive within macrophages so that other bacterial products cause sustained TNF-alpha gene transcription. Both Ly+ and Ly- produced molecules, isolated by 50% ammonium sulfate, that induced cytokine production. In conclusion, we now report that Ly+ causes sustained transcription of the TNF-alpha gene and production of TNF-alpha by macrophages in vitro. We speculate that the TNF-alpha may activate endothelium and thus allow the recruitment of T cells to sites of infection. This may contribute to the ability of only LLO-producing Listeria to induce protective immunity.
Assuntos
Toxinas Bacterianas , Proteínas de Choque Térmico/imunologia , Proteínas Hemolisinas/imunologia , Listeria monocytogenes/patogenicidade , Listeriose/imunologia , Macrófagos Peritoneais/imunologia , Fator de Necrose Tumoral alfa/biossíntese , Animais , Feminino , Expressão Gênica , Camundongos , Camundongos Endogâmicos C3H , Fagocitose , RNA Mensageiro/genéticaRESUMO
To increase the number of mutationally defined genes on chromosome I from Saccharomyces cerevisiae, most of the FUN2 (Function Unknown Now) transcribed region was deleted by gene replacement. Strains containing the deletion were viable, but grew with a 20% longer generation time. The mutation was recessive. Mutant haploids were able to mate, and homozygous mutant diploids were able to sporulate, giving asci containing four viable ascospores. These results indicate FUN2 is dispensable for the life cycle of S. cerevisiae, but required for an optimal growth rate.
Assuntos
Cromossomos Fúngicos , Clonagem Molecular/métodos , DNA Fúngico/genética , Mutação , Saccharomyces cerevisiae/genética , Transcrição Gênica , Deleção Cromossômica , DNA Recombinante/metabolismo , Genes Fúngicos , Mapeamento por Restrição , Saccharomyces cerevisiae/crescimento & desenvolvimentoRESUMO
SPO7 is one of several previously identified genes from the yeast Saccharomyces cerevisiae that is required for sporulation but not for vegetative growth. The SPO7 gene has been cloned by functional complementation and physically mapped 15-16 kb to the left of CEN1. Gene-disruption experiments confirmed that the cloned gene was the bona fide SPO7 gene. SPO7 codes for a 0.95-kb transcript that is expressed at approximately the same level in both vegetative and sporulating cells. The gene was sequenced and has the capacity to encode a 259-amino acid protein that does not appear to be related to other known proteins.
Assuntos
DNA Fúngico , Proteínas de Membrana , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Esporos/genética , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , Southern Blotting , Clonagem Molecular , DNA/isolamento & purificação , Proteínas Fúngicas/genética , Expressão Gênica , Dados de Sequência Molecular , Mapeamento por Restrição , Saccharomyces cerevisiae/fisiologia , Transcrição GênicaRESUMO
Three experiments tested the hypotheses that daily cortisol rhythm, feeding time, and/or insulin infusion affect(s) leptin secretion in stallions. Ten mature stallions received ad libitum hay and water and were fed a grain concentrate once daily at 0700. In Exp. 1, stallions received either a single injection of dexamethasone (125 microg/kg BW i.m.; n = 5) or vehicle (controls; n = 5) at 0700 on d -1. Starting 24 h later, blood samples were collected every 2 h for 36 h via jugular venipuncture. Cortisol in control stallions varied (P < 0.01) with time, with a morning peak and evening nadir; dexamethasone suppressed (P < 0.01) cortisol concentrations. Leptin and insulin were greater (P < 0.01) in the treated stallions, as was the insulin response to feeding (P < 0.01). Leptin in control stallions varied (P < 0.01) in a diurnal pattern, peaking approximately 10 h after onset of eating. This pattern of leptin secretion was similar, although of greater magnitude (P < 0.01), in treated stallions. In Exp. 2, five stallions were fed the concentrate portion of their diet daily at 0700 and five were switched to feeding at 1900. After 14 d on these regimens, blood samples were collected every 4 h for 48 h and then twice daily for 5 d. Cortisol varied diurnally (P = 0.02) and was not altered (P = 0.21) by feeding time. Insulin and leptin increased (P < 0.01) after feeding, and the peaks in insulin and leptin were shifted 12 h by feeding at 1900. In Exp. 3, six stallions were used in two 3 x 3 Latin square experiments. Treatments were 1) normal daily meal at 0700; 2) no feed for 24 h; and 3) no feed and a bolus injection of insulin (0.4 mIU/kg BW i.v.) followed by infusion of insulin (1.2 mIU.kg BW(-1).min(-1)) for 180 min, which was gradually decreased to 0 by 240 min; sufficient glucose was infused to maintain euglycemia. Plasma insulin increased (P < 0.01) in stallions when they were meal-fed (to approximately 150 microIU/mL) or infused with insulin and glucose (to approximately 75 microIU/mL), but insulin remained low (10 microIU/mL or less) when they were not fed. The increases in insulin were paralleled by gradual increases (P < 0.01) in leptin concentrations 3 to 4 h later in stallions fed or infused with insulin and glucose. When stallions were not fed, leptin concentrations remained low. These results demonstrate that feeding time, and more specifically the insulin increase associated with a meal, not cortisol rhythm, drives the postprandial increase in plasma leptin concentrations in horses.
Assuntos
Dexametasona/farmacologia , Glucocorticoides/farmacologia , Cavalos/fisiologia , Hipoglicemiantes/farmacologia , Insulina/farmacologia , Leptina/sangue , Ração Animal , Animais , Comportamento Alimentar , Hidrocortisona/metabolismo , Leptina/metabolismo , Masculino , Periodicidade , Período Pós-Prandial , Fatores de TempoRESUMO
The efficacy of antiviral drugs and vaccines administered intranasally may depend upon the technique of application. The distribution and time-course of removal of human serum albumin-technetium 99m (HSA-Tc 99m)-instilled intranasally were studied in eleven healthy volunteers using a gamma camera and an anterior sodium iodide scintillation detector. In 100 randomized studies material was delivered as drops in the supine position or as a spray to seated subjects. A significantly higher proportion of 'good' distributions (62 in 73 tests) was obtained with drops compared with spray (1 in 27). The volume administered was varied between 0.10 ml and 0.75 ml and the concentration of HSA was changed from 3 to 30% with no significant effect upon the distribution of time-course of removal; pertechnetate in isotonic saline was distributed and removed in a manner comparable to HSA-Tc 99m. Activity recorded by the detector showed an initial rapid fall associated with removal of most of the material from the nasal cavity, followed by a slower decline associated with the removal of material mainly from the anterior region of the nose. A multidose study confirmed that frequent administration by drops is required to maintain a high level of activity in the nasal cavity. Using this technique it should be possible to correlate measurements of antiviral efficacy and vaccines take-rates with certain characteristics of intranasal applicators; such studies may lead to the design of better devices.
Assuntos
Administração Intranasal , Albumina Sérica/metabolismo , Adulto , Estudos de Avaliação como Assunto , Humanos , Métodos , Cavidade Nasal/metabolismo , Mucosa Nasal/metabolismo , Soluções , Tecnécio , Vacinas/administração & dosagemRESUMO
The ADE1 gene of Saccharomyces cerevisiae was isolated by complementation in S. cerevisiae from a yeast genomic DNA library carried on plasmid YEp13. Electron microscopy of R-loop-containing DNA indicated the location of the ADE1 gene on the plasmid insert. Gene disruption and gene replacement were used to demonstrate that the ade1-complementing sequence was the actual ADE1 gene that maps on chromosome I. ade1 strains which normally form red colonies form white ones when transformed with the cloned ADE1 gene. This property should be very useful, since it enables detection of plasmids carrying this gene under nonselective conditions.
Assuntos
Cromossomos/fisiologia , Clonagem Molecular , DNA Fúngico/genética , Genes Fúngicos , Saccharomyces cerevisiae/genética , Enzimas de Restrição do DNA , Teste de Complementação Genética , Ligação Genética , Microscopia Eletrônica , Conformação de Ácido Nucleico , PlasmídeosRESUMO
The effects of changing retinal ganglion cell (RGC) density and availability of presynaptic sites on the development of RGC dendritic arbor in the developing chick retina were contrasted. Visual form deprivation was used to induce ocular enlargement and expanded retinal area resulting in a 20-30% decrease in RGC density. In these retinas, RGC dendritic arbors increased in a compensatory manner to maintain the inner nuclear layer to RGC convergence ratio in a way that is consistent with simple stretching; RGC dendritic arbors become larger with increased branch lengths, but without change in the total number of branches. In the second manipulation, partial optic nerve section was used to produce areas of RGC depletion of approximately 60% in the central retina. This reduction in density is comparable to the density of locations in the normal peripheral retina. In RGC depleted retinas, dendritic arbor areas of RGCs in the central retina grow to match the size of normal peripheral arbors. In contrast to the expanded case, two measures of intrinsic arbor structure are changed in RGC-depleted retinas; the branch density of RGC dendrites is greater, and the relative areas of the two arbors of bistratified cells are altered. We discuss the potential roles of retinal growth, local RGC density, and availability of presynaptic terminals in the developmental control of RGC dendritic arbor.
Assuntos
Dendritos/fisiologia , Terminações Pré-Sinápticas/fisiologia , Retina/crescimento & desenvolvimento , Células Ganglionares da Retina/citologia , Visão Ocular/fisiologia , Animais , Contagem de Células , GalinhasRESUMO
Candidate live-virus vaccines for respiratory syncytial virus are being developed and are beginning to be evaluated in clinical trials. To distinguish candidate vaccine strains from wild-type strains isolated during these trials, we developed PCR assays specific to two sets of candidate vaccine strains. The two sets were a group A strain (3A), its three attenuated, temperature-sensitive variant strains, a group B strain (2B), and its four attenuated, temperature-sensitive variant strains. The PCR assays were evaluated by testing 18 group A wild-type strains, the 3A strains, 9 group B wild-type strains, and the 2B strains. PCR specific to group A wild-type strains amplified only group A wild-type strains, and 3A-specific PCR amplified only 3A strains. PCR specific to group B wild-type strains amplified all group A and group B strains but gave a 688-bp product for group B wild-type strains, a 279-bp product for 2B strains, a 547-bp product for all group A strains, and an additional 688-bp product for some group A strains, including 3A strains. These types of PCR assays can, in conjunction with other methods, be used to efficiently distinguish candidate vaccine strains from other respiratory syncytial virus strains.
Assuntos
Reação em Cadeia da Polimerase/métodos , Vírus Sinciciais Respiratórios/genética , Vírus Sinciciais Respiratórios/imunologia , Vacinas Virais/genética , Sequência de Bases , Primers do DNA/genética , DNA Viral/genética , Variação Genética , Humanos , Dados de Sequência Molecular , Infecções por Vírus Respiratório Sincicial/prevenção & controle , Vírus Sinciciais Respiratórios/classificação , Especificidade da Espécie , Vacinas Atenuadas/genéticaRESUMO
Poliovirus vectors are being studied as potential vaccine delivery systems, with foreign genetic sequences incorporated as part of the viral genome. The foreign sequences are expressed as part of the viral polyprotein. Addition of proteolytic cleavage sites at the junction of the foreign polypeptide and the viral proteins results in cleavage during polyprotein processing. The ability of foot-and-mouth disease virus (FMDV) 2A to mediate proteolytic cleavage in the context of poliovirus vectors was studied. The results demonstrate that FMDV 2A is able to generate cleavage of the foreign antigen from the viral polyprotein. A second cleavage event between the FMDV 2A peptide and the foreign protein was also observed.
Assuntos
Antígenos Virais/metabolismo , Aphthovirus/enzimologia , Cisteína Endopeptidases/metabolismo , Vetores Genéticos , Vacina Antipólio Oral/genética , Vacinas Atenuadas/genética , Proteínas do Envelope Viral/metabolismo , Proteínas Virais , Sequência de Aminoácidos , Antígenos Virais/genética , Aphthovirus/genética , Cisteína Endopeptidases/genética , Técnicas de Transferência de Genes , Engenharia Genética , Células HeLa , Humanos , Dados de Sequência Molecular , Proteínas do Envelope Viral/genéticaRESUMO
We have determined the nucleotide sequence of the measles virus (MV) L gene using a cDNA library encompassing the entire MV genome (J. Crowley et al. (1987) Intervirology, 28, 65-77). The L gene is 6639 nucleotides in length, and contains a single long open reading frame that could code for a protein of 247,611 kDa. Both the L gene and in particular the predicted L protein of MV bear substantial homology to their counterparts in Sendai virus and Newcastle disease virus, suggesting that the multifunctional nature of paramyxovirus L proteins imposes strong evolutionary constraints. The predicted MV L protein also contains distinct elements of a postulated ancestral RNA polymerase.
Assuntos
RNA Polimerases Dirigidas por DNA/genética , Genes Virais , Vírus do Sarampo/genética , RNA Polimerase Dependente de RNA , Proteínas Virais/genética , Sequência de Aminoácidos , Sequência de Bases , Evolução Biológica , Clonagem Molecular , Endonucleases/farmacologia , Dados de Sequência Molecular , Homologia de Sequência do Ácido Nucleico , Endonucleases Específicas para DNA e RNA de Cadeia SimplesRESUMO
MAK16 is an essential gene on chromosome I defined by the thermosensitive lethal mak16-1 mutation. MAK16 is also necessary for M double-stranded RNA replication at the permissive temperature for cell growth. As part of an effort to clone all the DNA from chromosome I, plasmids that complemented both the temperature-sensitive growth defect, and the M1 replication defects of mak16-1 strains were isolated from a plasmid YCp50: Saccharomyces cerevisiae recombinant DNA library. The two plasmids analysed contained overlapping inserts that hybridized proportionally to strains carrying different dosages of chromosome I. Furthermore, integration of a fragment of one of these clones occurred at a site linked to ade 1, confirming that this clone was derived from the appropriate region of chromosome I. An open reading frame adjacent to MAK16 potentially coding for a 468 amino acid protein was defined by sequence analysis. 185 amino acids of this open reading frame were replaced with a 1.2 kb fragment carrying the S. cerevisiae URA3 gene by a one-step gene disruption. The resulting strains grew at a rate indistinguishable from the wild type at 20 degrees C, 30 degrees C, or 37 degrees C, but could not grow at 8 degrees C. The deleted region is thus essential only at 8 degrees C, and we name this gene LTE1 (low temperature essential).