RESUMO
The kinetochore is a complex structure whose function is absolutely essential. Unlike the centromere, the kinetochore at first appeared remarkably well conserved from yeast to humans, especially the microtubule-binding outer kinetochore. However, recent efforts towards biochemical reconstitution of diverse kinetochores challenge the notion of a similarly conserved architecture for the constitutively centromere-associated network of the inner kinetochore. This review briefly summarizes the evidence from comparative genomics for interspecific variability in inner kinetochore composition and focuses on novel biochemical evidence indicating that even homologous inner kinetochore protein complexes are put to different uses in different organisms.
Assuntos
Cinetocoros/metabolismo , Animais , Centrômero/genética , Centrômero/metabolismo , Genoma , Humanos , Cinetocoros/química , Mitose/fisiologia , Leveduras/genética , Leveduras/metabolismoRESUMO
Although rare, a recessive temperature-sensitive calmodulin mutant has been isolated in Saccharomyces cerevisiae. The mutant carries two mutations in CMD1, isoleucine 100 is changed to asparagine and glutamic acid 104 is changed to valine. Neither mutation alone conferred temperature sensitivity. A single mutation that allowed production of an intact but defective protein was not identified. At the nonpermissive temperature, the temperature-sensitive mutant displayed multiple defects. Bud formation and growth was delayed, but this defect was not responsible for the temperature-sensitive lethality. Cells synchronized in G1 progressed through the cell cycle and retained viability until the movement of the nucleus to the neck between the mother cell and the large bud. After nuclear movement, less than 5% of the cells survived the first mitosis and could form colonies when returned to permissive conditions. The duplicated DNA was dispersed along the spindle, extending from mother to daughter cell. Cells synchronized in G2/M lost viability immediately upon the shift to the nonpermissive temperature. At a semipermissive temperature, the mutant showed approximately a 10-fold increase in the rate of chromosome loss compared to a wild-type strain. The mitotic phenotype is very similar to yeast mutants that are defective in chromosome disjunction. The mutant also showed defects in cytokinesis.
Assuntos
Calmodulina/fisiologia , Mitose/fisiologia , Saccharomyces cerevisiae/citologia , Calmodulina/genética , Ciclo Celular , Divisão Celular/fisiologia , Cromossomos Fúngicos , Citometria de Fluxo , Fase G1 , Cinética , Mitose/genética , Mutação , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/isolamento & purificação , TemperaturaRESUMO
Calmodulin was localized in Saccharomyces cerevisiae by indirect immunofluorescence using affinity-purified polyclonal antibodies. Calmodulin displays an asymmetric distribution that changes during the cell cycle. In unbudded cells, calmodulin concentrates at the presumptive site of bud formation approximately 10 min before bud emergence. In small budded cells, calmodulin accumulates throughout the bud. As the bud grows, calmodulin concentrates at the tip, then disperses, and finally concentrates in the neck region before cytokinesis. An identical staining pattern is observed when wild-type calmodulin is replaced with mutant forms of calmodulin impaired in binding Ca2+. Thus, the localization of calmodulin does not depend on its ability to bind Ca2+ with a high affinity. Double labeling of yeast cells with affinity-purified anti-calmodulin antibody and rhodamine-conjugated phalloidin indicates that calmodulin and actin concentrate in overlapping regions during the cell cycle. Furthermore, disrupting calmodulin function using a temperature-sensitive calmodulin mutant delocalizes actin, and act1-4 mutants contain a random calmodulin distribution. Thus, calmodulin and actin distributions are interdependent. Finally, calmodulin localizes to the shmoo tip in cells treated with alpha-factor. This distribution, at sites of cell growth, implicates calmodulin in polarized cell growth in yeast.
Assuntos
Calmodulina/fisiologia , Saccharomyces cerevisiae/crescimento & desenvolvimento , Actinas/genética , Actinas/metabolismo , Especificidade de Anticorpos , Calmodulina/imunologia , Calmodulina/metabolismo , Polaridade Celular/fisiologia , Imunofluorescência , Immunoblotting , Mutação , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , TemperaturaRESUMO
In Saccharomyces cerevisiae, the unconventional myosin Myo2p is of fundamental importance in polarized growth. We explore the role of the neck region and its associated light chains in regulating Myo2p function. Surprisingly, we find that precise deletion of the six IQ sites in the neck region results in a myosin, Myo2-Delta6IQp, that can support the growth of a yeast strain at 90% the rate of a wild-type isogenic strain. We exploit this mutant in a characterization of the light chains of Myo2p. First, we demonstrate that the localization of calmodulin to sites of polarized growth largely depends on the IQ sites in the neck of Myo2p. Second, we demonstrate that a previously uncharacterized protein, Mlc1p, is a myosin light chain of Myo2p. MLC1 (YGL106w) is an essential gene that exhibits haploinsufficiency. Reduced levels of MYO2 overcome the haploinsufficiency of MLC1. The mutant MYO2-Delta6IQ is able to suppress haploinsufficiency but not deletion of MLC1. We used a modified gel overlay assay to demonstrate a direct interaction between Mlc1p and the neck of Myo2p. Overexpression of MYO2 is toxic, causing a severe decrease in growth rate. When MYO2 is overexpressed, Myo2p is fourfold less stable than in a wild-type strain. High copies of MLC1 completely overcome the growth defects and increase the stability of Myo2p. Our results suggest that Mlc1p is responsible for stabilizing this myosin by binding to the neck region.
Assuntos
Proteínas de Transporte/fisiologia , Proteínas Fúngicas/fisiologia , Cadeias Pesadas de Miosina , Cadeias Leves de Miosina/fisiologia , Miosina Tipo II , Miosina Tipo V , Miosinas/fisiologia , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/fisiologia , Proteínas de Schizosaccharomyces pombe , Sequência de Aminoácidos , Calmodulina/metabolismo , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Dados de Sequência Molecular , Cadeias Leves de Miosina/genética , Miosinas/química , Saccharomyces cerevisiae/metabolismo , Homologia de Sequência de AminoácidosRESUMO
Myo2p is an unconventional myosin required for polarized growth in Saccharomyces cerevisiae. Four lines of evidence suggest that (a) Myo2p is a target of calmodulin at sites of cell growth, and (b) the interaction between Myo2p and calmodulin is Ca2+ independent. First, as assessed by indirect immunofluorescence, the distributions of Myo2p and calmodulin are nearly indistinguishable throughout the cell cycle. Second, a genetic analysis indicates that mutations in CMD1 show allele-specific synthetic lethality with the myo2-66 conditional mutation. Mutations that inactivate the Ca(2+)-binding sites of calmodulin have little or no effect on strains carrying myo2-66, whereas an allele with a mutation outside the Ca(2+)-binding sites dramatically increases the severity of the phenotype conferred by myo2-66. Third, Myo2p coimmunoprecipitates with calmodulin in the presence of Ca2+ or EGTA. Finally, we used a modified gel overlay assay to demonstrate direct interaction between calmodulin and fusion proteins containing portions of Myo2p. Calmodulin binds specifically to the region of Myo2p containing six tandem repeats of a motif called an IQ site. Binding occurs in either Ca2+ or EGTA, and only two sites are required to observe binding.
Assuntos
Calmodulina/metabolismo , Proteínas de Transporte/metabolismo , Proteínas Fúngicas/metabolismo , Cadeias Pesadas de Miosina , Miosina Tipo II , Miosina Tipo V , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/metabolismo , Proteínas de Schizosaccharomyces pombe , Alelos , Sequência de Bases , Cálcio/metabolismo , Calmodulina/genética , Proteínas de Transporte/genética , Divisão Celular , Proteínas do Citoesqueleto , Ácido Egtázico/farmacologia , Imunofluorescência , Proteínas Fúngicas/genética , Dados de Sequência Molecular , Mutação , Proteínas Recombinantes de Fusão/metabolismo , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimentoRESUMO
Previously we demonstrated that calmodulin binds to the carboxy terminus of Spc110p, an essential component of the Saccharomyces cerevisiae spindle pole body (SPB), and that this interaction is required for chromosome segregation. Immunoelectron microscopy presented here shows that calmodulin and thus the carboxy terminus of Spc110p localize to the central plaque. We created temperature-sensitive SPC110 mutations by combining PCR mutagenesis with a plasmid shuffle strategy. The temperature-sensitive allele spc110-220 differs from wild type at two sites. The cysteine 911 to arginine mutation resides in the calmodulin-binding site and alone confers a temperature-sensitive phenotype. Calmodulin overproduction suppresses the temperature sensitivity of spc110-220. Furthermore, calmodulin levels at the SPB decrease in the mutant cells at the restrictive temperature. Thus, calmodulin binding to Spc110-220p is defective at the nonpermissive temperature. Synchronized mutant cells incubated at the nonpermissive temperature arrest as large budded cells with a G2 content of DNA and suffer considerable lethality. Immunofluorescent staining demonstrates failure of nuclear DNA segregation and breakage of many spindles. Electron microscopy reveals an aberrant nuclear structure, the intranuclear microtubule organizer (IMO), that differs from a SPB but serves as a center of microtubule organization. The IMO appears during nascent SPB formation and disappears after SPB separation. The IMO contains both the 90-kD and the mutant 110-kD SPB components. Our results suggest that disruption of the calmodulin Spc110p interaction leads to the aberrant assembly of SPB components into the IMO, which in turn perturbs spindle formation.
Assuntos
Calmodulina/metabolismo , Proteínas Fúngicas/metabolismo , Microtúbulos/química , Proteínas Nucleares/metabolismo , Proteínas de Saccharomyces cerevisiae , Fuso Acromático/química , Sítios de Ligação , Calmodulina/análise , Calmodulina/biossíntese , Proteínas de Ligação a Calmodulina , Ciclo Celular/fisiologia , Núcleo Celular/química , Cromossomos Fúngicos , Proteínas do Citoesqueleto , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Microtúbulos/ultraestrutura , Mutação , Proteínas Nucleares/genética , Proteínas Nucleares/fisiologia , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/crescimento & desenvolvimento , Fuso Acromático/ultraestrutura , Supressão Genética , TemperaturaRESUMO
Spc110p (Nuf1p) is an essential component of the yeast microtubule organizing center, or spindle pole body (SPB). Asynchronous wild-type cultures contain two electrophoretically distinct isoforms of Spc110p as detected by Western blot analysis, suggesting that Spc110p is modified in vivo. Both isoforms incorporate 32Pi in vivo, suggesting that Spc110p is post-translationally modified by phosphorylation. The slower-migrating 120-kD Spc110p isoform after incubation is converted to the faster-migrating 112-kD isoform after incubation with protein phosphatase PP2A, and specific PP2A inhibitors block this conversion. Thus, additional phosphorylation of Spc110p at serine and/or threonine residues gives rise to the slower-migrating 120-kD isoform. The 120-kD isoform predominates in cells arrested in mitosis by the addition of nocodazole. However, the 120-kD isoform is not detectable in cells grown to stationary phase (G0) or in cells arrested in G1 by the addition of alpha-factor. Temperature-sensitive cell division cycle (cdc) mutations demonstrate that the presence of the 120-kD isoform correlates with mitotic spindle formation but not with SPB duplication. In a synchronous wild-type population, the additional serine/threonine phosphorylation that gives rise to the 120-kD isoform appears as cells are forming the mitotic spindle and diminishes as cells enter anaphase. None of several sequences similar to the consensus for phosphorylation by the Cdc28p (cdc2p34) kinase is important for these mitosis-specific phosphorylations or for function. Carboxy-terminal Spc110p truncations lacking the calmodulin binding site can support growth and are also phosphorylated in a cell cycle-specific manner. Further truncation of the Spc110p carboxy terminus results in mutant proteins that are unable to support growth and now migrate as single species. Collectively, these results provide the first evidence of a structural component of the SPB that is phosphorylated during spindle formation and dephosphorylated as cells enter anaphase.
Assuntos
Ciclo Celular/fisiologia , Centrossomo/fisiologia , Proteínas Fúngicas/metabolismo , Proteínas Nucleares/metabolismo , Fosfoproteínas/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/fisiologia , Fuso Acromático/fisiologia , Anáfase/fisiologia , Proteína Quinase CDC28 de Saccharomyces cerevisiae/metabolismo , Proteínas de Ligação a Calmodulina , Ciclinas/metabolismo , Proteínas do Citoesqueleto , Mitose/fisiologia , Peso Molecular , Mutação , Fosforilação , Fosfosserina , Fosfotreonina , Relação Estrutura-AtividadeRESUMO
Many genes required for cell polarity development in budding yeast have been identified and arranged into a functional hierarchy. Core elements of the hierarchy are widely conserved, underlying cell polarity development in diverse eukaryotes. To enumerate more fully the protein-protein interactions that mediate cell polarity development, and to uncover novel mechanisms that coordinate the numerous events involved, we carried out a large-scale two-hybrid experiment. 68 Gal4 DNA binding domain fusions of yeast proteins associated with the actin cytoskeleton, septins, the secretory apparatus, and Rho-type GTPases were used to screen an array of yeast transformants that express approximately 90% of the predicted Saccharomyces cerevisiae open reading frames as Gal4 activation domain fusions. 191 protein-protein interactions were detected, of which 128 had not been described previously. 44 interactions implicated 20 previously uncharacterized proteins in cell polarity development. Further insights into possible roles of 13 of these proteins were revealed by their multiple two-hybrid interactions and by subcellular localization. Included in the interaction network were associations of Cdc42 and Rho1 pathways with proteins involved in exocytosis, septin organization, actin assembly, microtubule organization, autophagy, cytokinesis, and cell wall synthesis. Other interactions suggested direct connections between Rho1- and Cdc42-regulated pathways; the secretory apparatus and regulators of polarity establishment; actin assembly and the morphogenesis checkpoint; and the exocytic and endocytic machinery. In total, a network of interactions that provide an integrated response of signaling proteins, the cytoskeleton, and organelles to the spatial cues that direct polarity development was revealed.
Assuntos
Polaridade Celular/fisiologia , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/metabolismo , Actinas/metabolismo , Proteínas de Bactérias/genética , Endocitose/fisiologia , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Genes cdc/fisiologia , Proteínas Luminescentes/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae , Vesículas Secretórias/metabolismo , Técnicas do Sistema de Duplo-Híbrido , Proteína cdc42 de Saccharomyces cerevisiae de Ligação ao GTP/genética , Proteína cdc42 de Saccharomyces cerevisiae de Ligação ao GTP/metabolismo , Proteínas rho de Ligação ao GTP/metabolismoRESUMO
The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone alpha-factor. The 50% lethal dose (LD50) of alpha-factor for the calmodulin mutant is almost fivefold below the LD50 for a wild-type strain. The calmodulin mutants are not more sensitive to alpha-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca2+-calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD50 of alpha-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to alpha-factor. Activation of the Ca2+-calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD50 of alpha-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes.
Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/fisiologia , Cálcio/fisiologia , Proteínas de Ligação a Calmodulina/fisiologia , Calmodulina/fisiologia , Proteínas Fúngicas/fisiologia , Peptídeos/fisiologia , Fosfoproteínas Fosfatases/fisiologia , Saccharomyces cerevisiae/efeitos dos fármacos , Sequência de Bases , Calcineurina , Proteínas Quinases Dependentes de Cálcio-Calmodulina/genética , Calmodulina/genética , Ativação Enzimática , Fator de Acasalamento , Dados de Sequência Molecular , Saccharomyces cerevisiae/fisiologia , Deleção de SequênciaRESUMO
Two independent methods identified the spindle pole body component Nuf1p/Spc110p as the essential mitotic target of calmodulin. Extragenic suppressors of cmd1-1 were isolated and found to define three loci, XCM1, XCM2, and XCM3 (extragenic suppressor of cmd1-1). The gene encoding a dominant suppressor allele of XCM1 was cloned. On the basis of DNA sequence analysis, genetic cosegregation, and mutational analysis, XCM1 was identified as NUF1/SPC110. Independently, a C-terminal portion of Nuf1p/Spc110p, amino acid residues 828 to 944, was isolated as a calmodulin-binding protein by the two-hybrid system. As assayed by the two-hybrid system, Nuf1p/Spc110p interacts with wild-type calmodulin and triple-mutant calmodulins defective in binding Ca2+ but not with two mutant calmodulins that confer a temperature-sensitive phenotype. Deletion analysis by the two-hybrid system mapped the calmodulin-binding site of Nuf1p/Spc110p to amino acid residues 900 to 927. Direct binding between calmodulin and Nuf1p/Spc110p was demonstrated by a modified gel overlay assay. Furthermore, indirect immunofluorescence with fixation procedures known to aid visualization of spindle pole body components localized calmodulin to the spindle pole body. Sequence analysis of five suppressor alleles of NUF1/SPC110 indicated that suppression of cmd1-1 occurs by C-terminal truncation of Nuf1p/Spc110p at amino acid residues 856, 863, or 881, thereby removing the calmodulin-binding site.
Assuntos
Calmodulina/metabolismo , Mitose/fisiologia , Saccharomyces cerevisiae/metabolismo , Fuso Acromático/metabolismo , Sítios de Ligação , Calmodulina/genética , Clonagem Molecular , DNA Fúngico/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Genes Fúngicos , Genes Supressores , Imuno-Histoquímica , Mitose/genética , Mutagênese Sítio-Dirigida , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/genéticaRESUMO
The cmd1-1 mutation of calmodulin causes temperature-sensitive growth in Saccharomyces cerevisiae. We have isolated a dosage-dependent suppressor of cmd1-1, designated HCM1. Twentyfold overexpression of HCM1 permits strains carrying cmd1-1 to grow at temperatures up to and including 34 degrees C but does not suppress the lethality of either cmd1-1 at higher temperatures or the deletion of CMD1. Thus, overexpression of HCM1 does not bypass the requirement for calmodulin but enhances the ability of the mutant calmodulin to function. HCM1 is not essential for growth, but deletion of HCM1 exacerbates the phenotype of a strain carrying cmd1-1. HCM1 is located on chromosome III, which was recently sequenced. Our results correct errors in the published DNA sequence. The putative polypeptide encoded by HCM1 is 564 amino acids long and has a predicted molecular weight of 63,622. Antisera prepared against Hcm1p detect a protein that is overproduced in yeast strains overexpressing HCM1 and has an apparent molecular mass of 65 kDa. Eighty-six amino acid residues in the N terminus of Hcm1p show 50% identity with a DNA-binding region of the fork head family of DNA-binding proteins. When fused to the DNA-binding domain of Gal4p, residues 139 to 511 of Hcm1p can act as a strong activator of transcription. However, overexpression of HCM1 does not affect the expression of calmodulin. Furthermore, Hcm1p does not bind to calmodulin in a gel overlay assay. Thus, overexpression of HCM1 enhances calmodulin function by an apparently indirect mechanism.
Assuntos
Calmodulina/genética , Proteínas de Ligação a DNA/genética , Genes Supressores/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Sequência de Aminoácidos , Sequência de Bases , Calmodulina/metabolismo , Divisão Celular , Análise Mutacional de DNA , Proteínas de Ligação a DNA/biossíntese , Fatores de Transcrição Forkhead , Fator 3-alfa Nuclear de Hepatócito , Fator 3-beta Nuclear de Hepatócito , Fator 3-gama Nuclear de Hepatócito , Temperatura Alta , Dados de Sequência Molecular , Proteínas Nucleares/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/genética , Transcrição GênicaRESUMO
The central coiled coil of the essential spindle pole component Spc110p spans the distance between the central and inner plaques of the Saccharomyces cerevisiae spindle pole body (SPB). The carboxy terminus of Spc110p, which binds calmodulin, resides at the central plaque, and the amino terminus resides at the inner plaque from which nuclear microtubules originate. To dissect the functions of Spc110p, we created temperature-sensitive mutations in the amino and carboxy termini. Analysis of the temperature-sensitive spc110 mutations and intragenic complementation analysis of the spc110 alleles defined three functional regions of Spc110p. Region I is located at the amino terminus. Region II is located at the carboxy-terminal end of the coiled coil, and region III is the previously defined calmodulin-binding site. Overexpression of SPC98 suppresses the temperature sensitivity conferred by mutations in region I but not the phenotypes conferred by mutations in the other two regions, suggesting that the amino terminus of Spc110p is involved in an interaction with the gamma-tubulin complex composed of Spc97p, Spc98p, and Tub4p. Mutations in region II lead to loss of SPB integrity during mitosis, suggesting that this region is required for the stable attachment of Spc110p to the central plaque. Our results strongly argue that Spc110p links the gamma-tubulin complex to the central plaque of the SPB.
Assuntos
Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Mutação/genética , Proteínas Nucleares/química , Proteínas Nucleares/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Alelos , Sítios de Ligação , Calmodulina/metabolismo , Proteínas de Ligação a Calmodulina , Ciclo Celular/efeitos dos fármacos , Proteínas do Citoesqueleto , Proteínas Fúngicas/genética , Dosagem de Genes , Genes Essenciais/genética , Genes Fúngicos/genética , Genes Supressores/genética , Teste de Complementação Genética , Hidroxiureia/farmacologia , Microscopia Eletrônica , Proteínas Nucleares/genética , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/crescimento & desenvolvimento , Fuso Acromático/efeitos dos fármacos , Fuso Acromático/genética , Fuso Acromático/ultraestrutura , Temperatura , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismoRESUMO
The spindle pole body (SPB) in Saccharomyces cerevisiae functions as the microtubule-organizing center. Spc110p is an essential structural component of the SPB and spans between the central and inner plaques of this multilamellar organelle. The amino terminus of Spc110p faces the inner plaque, the substructure from which spindle microtubules radiate. We have undertaken a synthetic lethal screen to identify mutations that enhance the phenotype of the temperature-sensitive spc110-221 allele, which encodes mutations in the amino terminus. The screen identified mutations in SPC97 and SPC98, two genes encoding components of the Tub4p complex in yeast. The spc98-63 allele is synthetic lethal only with spc110 alleles that encode mutations in the N terminus of Spc110p. In contrast, the spc97 alleles are synthetic lethal with spc110 alleles that encode mutations in either the N terminus or the C terminus. Using the two-hybrid assay, we show that the interactions of Spc110p with Spc97p and Spc98p are not equivalent. The N terminus of Spc110p displays a robust interaction with Spc98p in two different two-hybrid assays, while the interaction between Spc97p and Spc110p is not detectable in one strain and gives a weak signal in the other. Extra copies of SPC98 enhance the interaction between Spc97p and Spc110p, while extra copies of SPC97 interfere with the interaction between Spc98p and Spc110p. By testing the interactions between mutant proteins, we show that the lethal phenotype in spc98-63 spc110-221 cells is caused by the failure of Spc98-63p to interact with Spc110-221p. In contrast, the lethal phenotype in spc97-62 spc110-221 cells can be attributed to a decreased interaction between Spc97-62p and Spc98p. Together, these studies provide evidence that Spc110p directly links the Tub4p complex to the SPB. Moreover, an interaction between Spc98p and the amino-terminal region of Spc110p is a critical component of the linkage, whereas the interaction between Spc97p and Spc110p is dependent on Spc98p.
Assuntos
Proteínas Fúngicas/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Proteínas Nucleares/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/fisiologia , Fuso Acromático/fisiologia , Tubulina (Proteína)/química , Tubulina (Proteína)/metabolismo , Proteínas de Ligação a Calmodulina , Proteínas do Citoesqueleto , Proteínas Fúngicas/genética , Teste de Complementação Genética , Marcadores Genéticos , Biblioteca Genômica , Genótipo , Proteínas Associadas aos Microtúbulos/genética , Modelos Moleculares , Mutagênese , Proteínas Nucleares/genética , Conformação Proteica , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/ultraestrutura , Fuso Acromático/genética , Fuso Acromático/ultraestruturaRESUMO
We previously identified HCM1 as a dosage-dependent suppressor of a calmodulin temperature-sensitive mutant (cmd1-1). Calmodulin performs multiple functions in yeast. Here we demonstrate that the effects of HCM1 are specific to the role of calmodulin at the spindle pole body. Overexpression of HCM1 fully suppresses the temperature sensitivity of a calmodulin mutant (cmd1-3) that only has defects in assembly of the spindle pole body but does not suppress the temperature sensitivity of a calmodulin mutant (cmd1-8) that only affects other functions of calmodulin. The DNA binding specificity of Hcm1p was determined by a selection, amplification and binding protocol. The consensus sequence for an Hcmlp binding site is WAAYAAACAAW. Mutations in the DNA binding domain of Hcm1p abolish the ability of Hcmlp to specifically recognize this binding site and abolish the ability of Hcm1p to act as a suppressor of calmodulin mutants. The promoter of SPC110 contains a match to the consensus binding site. Deletion of HCM1 does not affect the basal level of SPC110 transcription, but reduces the induction that occurs late in G1 of the cell cycle.
Assuntos
Proteínas de Ligação a Calmodulina/biossíntese , Proteínas de Ligação a DNA/fisiologia , Proteínas Fúngicas/biossíntese , Proteínas Nucleares/biossíntese , Proteínas de Saccharomyces cerevisiae , Fatores de Transcrição/fisiologia , Sequência de Aminoácidos , Calmodulina/genética , Sequência Consenso , Proteínas do Citoesqueleto , DNA/química , Proteínas de Ligação a DNA/química , Fatores de Transcrição Forkhead , Regulação da Expressão Gênica , Dados de Sequência Molecular , Mutação , Regiões Promotoras Genéticas , Alinhamento de Sequência , Fuso Acromático/metabolismo , Temperatura , LevedurasRESUMO
Calmodulin is well characterized as an intracellular Ca2+ receptor in nonproliferating tissues such as muscle and brain. Several observations indicate that calmodulin is also required for cellular growth and division. Deletion of the calmodulin gene is a lethal mutation in Saccharomyces cerevisiae, Schizosaccharomyces pombe and Aspergillus nidulans. Expression of calmodulin antisense RNA in mouse C127 cells causes a transient arrest at G1 and metaphase. Although these results indicate calmodulin plays a critical function during proliferation, they do not reveal the function. S. cerevisiae offers an excellent system for identifying calmodulin functions. Because calmodulin mutants can be readily constructed by gene replacement the consequences of mutations in calmodulin can be directly examined in vivo without interference from wild-type calmodulin. The available wealth of information concerning all aspects of the yeast life cycle provides a large framework for interpretation of new results. The recent dissection of cell cycle regulation is just the latest example of the important insights provided by analyzing basic cellular processes in yeast. Whether studies of calmodulin in yeast will reveal a universal function is unknown. One encouraging result is that yeast cells relying on vertebrate calmodulin as their only source of calmodulin survive and grow well, even if the amount of vertebrate calmodulin is equivalent to the normal steady state levels of yeast calmodulin. This review discusses the varied techniques we are using to identify the functions of calmodulin in yeast. As part of the analysis, we are defining the essential elements of calmodulin structure.
Assuntos
Calmodulina/fisiologia , Proteínas Fúngicas/genética , Saccharomyces cerevisiae/fisiologia , Calmodulina/genética , MutaçãoRESUMO
Calmodulin from Saccharomyces cerevisiae was expressed in Escherichia coli and purified. The purified protein was structurally characterized using limited proteolysis followed by ESI mass spectrometry to identify the fragments. In the presence of Ca2+, yeast calmodulin is sequentially cleaved at arginine 126, then lysine 115, and finally at lysine 77. The rapid cleavage at Arg-126 suggests that the fourth Ca(2+)-binding loop does not bind Ca2+. In the presence of EGTA, yeast calmodulin is more susceptible to proteolysis and is preferentially cleaved at Lys-106. In addition, mutant proteins carrying I100N, E104V or both mutations, which together confer temperature sensitivity to yeast, were characterized. The mutant proteins are more susceptible than wild-type calmodulin to proteolysis, suggesting that each mutation disrupts the structure of calmodulin. Furthermore, whereas wild-type calmodulin is cut at Lys-106 only in the presence of EGTA, this cleavage site is accessible in the mutants in the presence of Ca2+ as well. In these ways, the structural consequence of each mutation mimics the loss of a calcium ion in the third loop. In addition, although wild-type calmodulin binds to four proteins in a yeast crude extract in the presence of Ca2+, the mutants bind only to a subset of these. Thus, the inability to adopt the stable Ca(2+)-bound conformation in the third Ca(2+)-binding loop alters the ability of calmodulin to interact with yeast proteins in a Ca(2+)-dependent manner.
Assuntos
Calmodulina/química , Saccharomyces cerevisiae/química , Cálcio/metabolismo , Calmodulina/biossíntese , Calmodulina/genética , Escherichia coli/genética , Espectrometria de Massas , Mutação , Fragmentos de Peptídeos/química , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Saccharomyces cerevisiae/genéticaRESUMO
The newest generation of bonding systems consolidates multiple components into fewer containers in order to simplify clinical procedures and save clinicians time. Six newer adhesive systems (Scotchbond Multi-Purpose Plus, PROBOND, OptiBond FL, Prime & Bond, One Step, and Tenure Quik) were tested for in vitro shear bond strength (SBS) of a hybrid composite to both superficial and deep dentin at 24 hours and 6 months employing a pushout test method. Results showed significant differences in SBS between superficial and deep dentin for both 1-day and 6-month values for all adhesive systems except Tenure Quik. The SBS of OptiBond FL increased significantly for both superficial and deep dentin after 6 months' storage in 37 degrees C water. None of the other adhesive systems showed a significant change in SBS at 6 months. There appear to be several advantages to the testing method.
Assuntos
Colagem Dentária , Adesivos Dentinários/química , Condicionamento Ácido do Dente , Análise de Variância , Humanos , Técnicas In Vitro , Teste de Materiais/métodos , Teste de Materiais/estatística & dados numéricos , Propriedades de Superfície , Resistência à Tração , Fatores de TempoRESUMO
The effect of oral ambient air on shear strength to etched enamel and dentin was determined for OptiBond FL (Kerr Corp., Orange, CA) and Prodigy resin composite (Kerr Corp.). Enamel and dentin specimens of extracted human teeth were treated both in a dry environment and after exposure to oral humidity according to manufacturer's instructions. Shear strengths of this system are 22.6 MPa for etched, dry enamel; 22.2 MPa for etched, wet enamel; and 18.4 MPa for etched, dry or wet dentin. Differences in shear strengths between the wet and dry enamel or the wet and dry dentin were not significant. Multiple Student's t-tests were used for statistical analysis. Fracture modes for all specimens were examined under a stereomicroscope. Within the parameters of this in vitro study, OptiBond FL was not affected by oral humidity.