Detection of African swine fever virus in neonatal piglets with congenital tremors.

African swine fever virus (ASF) has circulated in Vietnam since 2018, causing significant losses to the pig industry. Quick, accurate diagnosis of African swine fever virus (ASFV) infection is crucial for controlling the disease. The detection of the virus in piglets with congenital tremors is described in this paper. ASFV was detected in brain tissues by polymerase chain reaction (PCR) and immunohistochemistry. Classical swine fever virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus were not detected by PCR, suggesting that the ASFV was the cause of these neurological signs.

Genetic Characterization of African Swine Fever Virus in Various Outbreaks in Central and Southern Vietnam During 2019-2021.

This study aimed to identify potential genetic diversity among African swine fever virus (ASFV) strains circulating in central and southern Vietnam. Thirty ASFV strains were collected from domestic pigs and convalescent pigs with ASFV-infected clinical signs from 19 different provinces of central and southern Vietnam during 2019-2021. A portion of the B646L (p72) gene and the entire E183L (p54), CP204L (p30), and B602L (CVR) genes were amplified, purified, and sequenced. Web-based BLAST and MEGA X software were used for sequence analysis. Analysis of the partial B646L (p72) gene, the full-length E183L (p54) and CP204L (p30) genes, and the central hypervariable region (CVR) of the B602L gene sequence showed that all 30 ASFV isolates belonged to genotype II and were 100% identical to the previously identified strains in Vietnam and China. Analysis of the p72, p54, and p30 regions did not indicate any change in the nucleotide and amino acid sequences among these strains in 3 years of research. No novel variant was found in the CVR within the B602L gene. Analysis of the CVR showed that these ASFV strains belong to subgroup XXXII. The results of this study revealed that these ASFVs shared high similarity with ASFV isolates detected previously in northern Vietnam and China. Taken together, the results of this study and a previous study in Vietnam showed high stability and no genetic diversity in the ASFV genome.

Factors Associated With Crestal Bone Loss Following Dental Implant Placement in a Longitudinal Follow-up Study.

The purpose of this study is to estimate the magnitude of crestal bone loss and to identify factors associated with changes in crestal bone height following placement of dental implants. This was a retrospective cohort study, consisting of a sample derived from the population of patients who had at least 1 dental implant placed in a community practice over a 10-year period. A total of 11 predictor variables were grouped into demographic, related health status, anatomic, implant-specific, and operative categories. The primary outcome variable was a change in crestal bone height (mm) over the course of follow-up. The secondary outcome variable was crestal bone loss at 1 year grouped into 2 categories (bone loss >1.5 mm and ≤1.5 mm). Univariate and multivariate regression mixed-effects models were developed to identify variables associated with crestal bone level changes over time. P values ≤.05 were considered statistically significant. The study sample was composed of 85 subjects who received 148 implants. The mean change of the crestal bone was -2.1 ± 1.5 mm (range = -12.5 to 0.5 mm; median = -1.77 mm). In the multivariate model, none of the variables studied were statistically associated with mean crestal bone loss. Among 84 (66.1%) implants with bone loss >1.5 mm within 1 year, no variables were associated with bone loss in the multivariate model. Of the 11 predictor variables evaluated in this study, none were statistically significant with regard to an increased risk for crestal bone loss or for excessive bone loss within the first year after implant placement.