Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros

Base de dados
Tipo de documento
Assunto da revista
País de afiliação
Intervalo de ano de publicação
1.
Neurosci Lett ; 732: 135072, 2020 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-32512036

RESUMO

Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) "Egr1-EGFP" reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein. This precautionary study outlines the limitations of the Egr1-EGFP transgenic line as a tool to study the activity-dependent expression of Egr1 and emphasizes the necessity of taking into account the potential loss of regulatory elements, stability determinants, or translational modulation in transgenic reporter strains.


Assuntos
Proteínas de Fluorescência Verde/metabolismo , Hipocampo/metabolismo , Camundongos Transgênicos , Animais , Córtex Cerebral/metabolismo , Expressão Gênica , Genes Reporter , Camundongos , Sistema Nervoso
2.
J Neurosci Methods ; 266: 151-60, 2016 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-27039972

RESUMO

BACKGROUND: Understanding the neurobiological basis of cognition and behavior, and disruptions to these processes following injury and disease, requires a large-scale assessment of neural populations, and knowledge of their patterns of connectivity. NEW METHOD: We present an analysis platform for large-scale investigation of functional and neuroanatomical connectivity in rodents. Retrograde tracers were injected and in a subset of animals behavioral tests to drive immediate-early gene expression were administered. This approach allows users to perform whole-brain assessment of function and connection in a semi-automated quantitative manner. Brains were cut in the coronal plane, and an image of the block face was acquired. Wide-field fluorescent scans of whole sections were acquired and analyzed using Matlab software. RESULTS: The toolkit utilized open-source and custom platforms to accommodate a largely automated analysis pipeline in which neuronal boundaries are automatically segmented, the position of segmented neurons are co-registered with a corresponding image acquired during sectioning, and a 3-D representation of neural tracer (and other products) throughout the entire brain is generated. COMPARISON WITH EXISTING METHODS: Current whole brain connectivity measures primarily target mice and use anterograde tracers. Our focus on segmented units of interest (e.g., NeuN labeled neurons) and restricting measures to these units produces a flexible platform for a variety of whole brain analyses (measuring activation, connectivity, markers of disease, etc.). CONCLUSIONS: This open-source toolkit allows an investigator to visualize and quantify whole brain data in 3-D, and additionally provides a framework that can be rapidly integrated with user-specific analyses and methodologies.


Assuntos
Mapeamento Encefálico/métodos , Encéfalo/citologia , Encéfalo/metabolismo , Expressão Gênica , Imageamento Tridimensional/métodos , Software , Animais , Feminino , Genes Precoces/fisiologia , Masculino , Camundongos Endogâmicos C57BL , Vias Neurais/citologia , Vias Neurais/metabolismo , Técnicas de Rastreamento Neuroanatômico/métodos , Reconhecimento Automatizado de Padrão/métodos , Ratos Endogâmicos F344 , Ratos Long-Evans
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA