RESUMO
PURPOSE: Gene expressions of vascular Endothelial Growth Factor Alpha (VEGFa), Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B cells (NFkB) and cytokines could be useful for identifying potential therapeutic targets to alleviate ischemia-reperfusion injury after liver transplantation. Cytokine gene expressions, VEGFa and NFkB were investigated in a preclinical swine model of liver transplantation. METHODS: A total of 12 pigs were used as donors and recipients in liver transplantation without venovenous bypass or aortic clamping. NFkB, IL-6, IL-10, VEGFa and Notch1 gene expression were assessed. These samples were collected in two specific times: group 1 (n= 6) - control, samples were collected before recipient's total hepatectomy and group 2 - liver transplantation group (n=6), where the samples were collected one hour after graft reperfusion. RESULTS: Liver transplantation was successfully performed in all recipients. Liver enzymes were elevated in the transplantation group. NFkB gene expression was significantly decreased in the transplantation group in comparison with the control group (0.62±0.19 versus 0.39±0.08; p= 0.016). No difference was observed between groups Interleucine 6 (IL-6), interleucine 10 (IL-10), VEGFa and Notch homolog 1 (Notch1). CONCLUSIONS: In this survey a decreased NFkB gene expression in a porcine model of liver transplantation was observed.
Assuntos
Transplante de Fígado , NF-kappa B , Fator A de Crescimento do Endotélio Vascular , Animais , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Fator A de Crescimento do Endotélio Vascular/análise , Suínos , NF-kappa B/metabolismo , Interleucina-10/análise , Interleucina-6/análise , Interleucina-6/genética , Traumatismo por Reperfusão , Expressão Gênica , Modelos Animais de Doenças , Receptor Notch1/genética , Citocinas , Fígado/metabolismo , Modelos Animais , MasculinoRESUMO
Purpose: Gene expressions of vascular Endothelial Growth Factor Alpha (VEGFa), Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B cells (NFkB) and cytokines could be useful for identifying potential therapeutic targets to alleviate ischemia-reperfusion injury after liver transplantation. Cytokine gene expressions, VEGFa and NFkB were investigated in a preclinical swine model of liver transplantation. Methods: A total of 12 pigs were used as donors and recipients in liver transplantation without venovenous bypass or aortic clamping. NFkB, IL-6, IL-10, VEGFa and Notch1 gene expression were assessed. These samples were collected in two specific times: group 1 (n= 6) - control, samples were collected before recipient's total hepatectomy and group 2 - liver transplantation group (n=6), where the samples were collected one hour after graft reperfusion. Results: Liver transplantation was successfully performed in all recipients. Liver enzymes were elevated in the transplantation group. NFkB gene expression was significantly decreased in the transplantation group in comparison with the control group (0.62±0.19 versus 0.39±0.08; p= 0.016). No difference was observed between groups Interleucine 6 (IL-6), interleucine 10 (IL-10), VEGFa and Notch homolog 1 (Notch1). Conclusions: In this survey a decreased NFkB gene expression in a porcine model of liver transplantation was observed.
Assuntos
Animais , Doenças dos Suínos , Traumatismo por Reperfusão , Expressão Gênica , Transplante de Fígado , Quinase Induzida por NF-kappaBRESUMO
La neoplasia sólida pseudopapilar de páncreas es una neoplasia maligna de bajo grado, relativamente poco frecuente, que representa entre 0.9 y 2.7 % de todas las neoplasias malignas pancreáticas. Afecta principalmente mujeres jóvenes (89 % de los casos), con una edad promedio al diagnóstico de 28 años. La mayoría presentan sintomatología inespecífica relacionada a una masa intrabdominal y más de un tercio suelen ser descubiertas incidentalmente. Presentamos dos casos (una paciente de 19 años de edad diagnosticada de una neoplasia localizada en cuerpo de páncreas y una paciente de 28 años con tumor en la cabeza, que correspondían a tumor de Frantz. Analizamos las características biológicas de estos tumores, sus formas de presentación y las pruebas complementarias recomendadas para su diagnóstico, así como una búsqueda en la literatura.
The solid pseudopapillary neoplasm of the pancreas is a low-grade malignancy, relatively rare, representing between 0.9 and 2.7% of all pancreatic malignancies. It mainly affects young women (89% of cases), with a mean age at diagnosis of 28 years. Most have specific symptoms related to intra-abdominal mass and more than one third are usually discovered incidentally. We report two cases ( a 19-years-old woman diagnosed of a neoplasm in the tail and another 28 years old woman , with neoplasm in the head of the pancreas which could be classified as Frantzs tumor. We analyze the biological characteristics of these tumors, their forms of presentation and the recommended explorations for the diagnostic.