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Environ Monit Assess ; 186(7): 4323-30, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24682661

RESUMO

The implementation of good agricultural practices (GAP) from irrigation water to the tomato packaging process enhances the safety of fresh produce and its value throughout the food chain. The aim of the present study was to show that fresh produce farms that apply and enforce GAP could reduce the presence of Salmonella in finished produce. Samples were collected biweekly from six packing houses from the central region of Sinaloa, México, for the isolation of Salmonella spp by the ISO 6579:2002 method, and the isolated strains were serotyped and genotyped by the Kauffmman-White scheme and pulsed field gel electrophoresis (PFGE), respectively. Salmonella strains were detected in 13 (36.1 %) irrigation water samples, while only two tomato samples were positive (5.5 %). Eight different serotypes were identified in irrigation water, and Salmonella Oranienburg (34 %) was the most prevalent; however, only Salmonella Agona and Salmonella Weltevreden were present on tomatoes. Salmonella Oranienburg was the most widely dispersed and variable serotype, with 10 different PFGE profiles. Salmonella Weltevreden was isolated from both types of samples, albeit with distinct genetic profiles, implying that the sources of contamination differ. These results confirm the utility of implementing good agricultural practices to reduce Salmonella contamination in irrigation water and the packaging process.


Assuntos
Agricultura/métodos , Solanum lycopersicum/crescimento & desenvolvimento , Qualidade da Água/normas , Monitoramento Ambiental , Microbiologia de Alimentos , Solanum lycopersicum/microbiologia , México , Salmonella/isolamento & purificação , Microbiologia do Solo , Eliminação de Resíduos Líquidos
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