RESUMO
Outer hair cells (OHCs) of the mammalian cochlea actively change their cell length in response to changes in membrane potential. This electromotility, thought to be the basis of cochlear amplification, is mediated by a voltage-sensitive motor molecule recently identified as the membrane protein prestin. Here, we show that voltage sensitivity is conferred to prestin by the intracellular anions chloride and bicarbonate. Removal of these anions abolished fast voltage-dependent motility, as well as the characteristic nonlinear charge movement ("gating currents") driving the underlying structural rearrangements of the protein. The results support a model in which anions act as extrinsic voltage sensors, which bind to the prestin molecule and thus trigger the conformational changes required for motility of OHCs.
Assuntos
Bicarbonatos/metabolismo , Cloretos/metabolismo , Células Ciliadas Auditivas Externas/fisiologia , Proteínas/metabolismo , Substituição de Aminoácidos , Animais , Proteínas de Transporte de Ânions , Ânions/farmacologia , Bicarbonatos/farmacologia , Células CHO , Cátions/farmacologia , Membrana Celular/metabolismo , Cloretos/farmacologia , Cricetinae , Condutividade Elétrica , Eletrofisiologia , Modelos Biológicos , Mutação , Técnicas de Patch-Clamp , Conformação Proteica , Proteínas/química , Proteínas/genética , Ratos , Transportadores de SulfatoRESUMO
Adenosine triphosphate (ATP)-sensitive potassium (KATP) channels couple electrical activity to cellular metabolism through their inhibition by intracellular ATP. ATP inhibition of KATP channels varies among tissues and is affected by the metabolic and regulatory state of individual cells, suggesting involvement of endogenous factors. It is reported here that phosphatidylinositol-4, 5-bisphosphate (PIP2) and phosphatidylinositol-4-phosphate (PIP) controlled ATP inhibition of cloned KATP channels (Kir6.2 and SUR1). These phospholipids acted on the Kir6.2 subunit and shifted ATP sensitivity by several orders of magnitude. Receptor-mediated activation of phospholipase C resulted in inhibition of KATP-mediated currents. These results represent a mechanism for control of excitability through phospholipids.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Trifosfato de Adenosina/farmacologia , Fosfatidilinositol 4,5-Difosfato/farmacologia , Fosfatos de Fosfatidilinositol/farmacologia , Bloqueadores dos Canais de Potássio , Canais de Potássio Corretores do Fluxo de Internalização , Trifosfato de Adenosina/metabolismo , Animais , Clonagem Molecular , Diazóxido/farmacologia , Relação Dose-Resposta a Droga , Mutação , Oócitos , Técnicas de Patch-Clamp , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Fosfatidilinositóis/farmacologia , Canais de Potássio/genética , Canais de Potássio/metabolismo , Receptores de Droga/metabolismo , Receptores Purinérgicos P2/metabolismo , Receptores Purinérgicos P2Y2 , Proteínas Recombinantes de Fusão/metabolismo , Receptores de Sulfonilureias , Fosfolipases Tipo C/metabolismo , Xenopus laevisRESUMO
Second messenger regulation of IRK1 (Kir2.1) inward rectifier K+ channels was investigated in giant inside-out patches from Xenopus oocytes. Kir2.1-mediated currents that run down completely within minutes upon excision of the patches could be partly restored by application of Mg-ATP together with > 10 microM free Mg2+ to the cytoplasmic side of the patch. As restoration could not be induced by the ATP analogs AMP-PNP or ATP gamma S, this suggests an ATPase-like mechanism. In addition to ATP, the catalytic subunit of cAMP-dependent protein kinase (PKA) induced an increase in current amplitude, which could, however, only be observed if channels were previously or subsequently stimulated by Mg-ATP and free Mg2+. This indicates that functional activity of Kir2.1 channels requires both phosphorylation by PKA and ATP hydrolysis. Moreover, currents could be down-regulated by N-heptyl-5-chloro-1-naphthalenesulfonamide, a specific stimulator of protein kinase C (PKC), suggesting that PKA and PKC mediate inverse effects on Kir2.1 channels. Regulation of Kir2.1 channels described here may be an important mechanism for regulation of excitability.
Assuntos
Trifosfato de Adenosina/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Ativação do Canal Iônico , Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/fisiologia , Proteína Quinase C/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Primers do DNA/química , Magnésio/metabolismo , Dados de Sequência Molecular , Oócitos , Fosforilação , Sistemas do Segundo Mensageiro , Xenopus laevisRESUMO
Fast inhibitory synaptic transmission in the central nervous system is mediated by ionotropic GABA or glycine receptors. Auditory outer hair cells present a unique inhibitory synapse that uses a Ca2+-permeable excitatory acetylcholine receptor to activate a hyperpolarizing potassium current mediated by small conductance calcium-activated potassium (SK) channels. It is shown here that unitary inhibitory postsynaptic currents at this synapse are mediated by SK2 channels and occur rapidly, with rise and decay time constants of approximately 6 ms and approximately 30 ms, respectively. This time course is determined by the Ca2+ gating of SK channels rather than by the changes in intracellular Ca2+. The results demonstrate fast coupling between an excitatory ionotropic neurotransmitter receptor and an inhibitory ion channel and imply rapid, localized changes in subsynaptic calcium levels.
Assuntos
Vias Auditivas/fisiologia , Cálcio/fisiologia , Células Ciliadas Auditivas Externas/fisiologia , Inibição Neural/fisiologia , Canais de Potássio/fisiologia , Transmissão Sináptica/fisiologia , Animais , Eletrofisiologia , Técnicas In Vitro , Ativação do Canal Iônico , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Small conductance Ca(2+)-activated potassium channels (SK channels) are coassembled complexes of pore-forming SK alpha subunits and calmodulin. We proposed a model for channel activation in which Ca2+ binding to calmodulin induces conformational rearrangements in calmodulin and the alpha subunits that result in channel gating. We now report fluorescence measurements that indicate conformational changes in the alpha subunit after calmodulin binding and Ca2+ binding to the alpha subunit-calmodulin complex. Two-hybrid experiments showed that the Ca(2+)-independent interaction of calmodulin with the alpha subunits requires only the C-terminal domain of calmodulin and is mediated by two noncontiguous subregions; the ability of the E-F hands to bind Ca2+ is not required. Although SK alpha subunits lack a consensus calmodulin-binding motif, mutagenesis experiments identified two positively charged residues required for Ca(2+)-independent interactions with calmodulin. Electrophysiological recordings of SK2 channels in membrane patches from oocytes coexpressing mutant calmodulins revealed that channel gating is mediated by Ca2+ binding to the first and second E-F hand motifs in the N-terminal domain of calmodulin. Taken together, the results support a calmodulin- and Ca(2+)-calmodulin-dependent conformational change in the channel alpha subunits, in which different domains of calmodulin are responsible for Ca(2+)-dependent and Ca(2+)-independent interactions. In addition, calmodulin is associated with each alpha subunit and must bind at least one Ca2+ ion for channel gating. Based on these results, a state model for Ca2+ gating was developed that simulates alterations in SK channel Ca2+ sensitivity and cooperativity associated with mutations in CaM.
Assuntos
Cálcio/fisiologia , Calmodulina/fisiologia , Canais de Potássio Cálcio-Ativados , Canais de Potássio/fisiologia , Animais , Cálcio/metabolismo , Calmodulina/genética , Calmodulina/metabolismo , Feminino , Ativação do Canal Iônico/fisiologia , Modelos Biológicos , Oócitos , Canais de Potássio/genética , Ratos , Canais de Potássio Ativados por Cálcio de Condutância Baixa , XenopusRESUMO
Subtypes of P2Y receptors are well characterized with respect to their agonist profile but little is known about differences in their intracellular signalling properties. When expressed in Xenopus oocytes, both P2Y2 and P2Y6 receptors effectively couple to endogenous Ca2+-dependent Cl--channels. However, only P2Y2 receptors increased currents mediated by inward-rectifier K+ channels of the Kir3.0 subfamily. This increase in Kir-current was sensitive to pertussis toxin, while activation of Ca2+-dependent Cl--channels was not. In contrast, suramin, a P2 receptor antagonist, inhibited activation of both channels. These observations suggest that, in contrast to P2Y6, P2Y2 receptors couple to two different classes of G proteins.
Assuntos
Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/metabolismo , Receptores Purinérgicos P2/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Cálcio/metabolismo , Canais de Cloreto , Eletrofisiologia/métodos , Feminino , Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G , Oócitos , Toxina Pertussis , Canais de Potássio/efeitos dos fármacos , Agonistas do Receptor Purinérgico P2 , Antagonistas do Receptor Purinérgico P2 , Receptores Purinérgicos P2/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Suramina/farmacologia , Uridina Trifosfato/farmacologia , Fatores de Virulência de Bordetella/farmacologia , Xenopus laevisRESUMO
Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.
Assuntos
Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/fisiologia , Espermina/farmacologia , Animais , Relação Dose-Resposta a Droga , Feminino , Técnicas In Vitro , Ativação do Canal Iônico/efeitos dos fármacos , Ativação do Canal Iônico/fisiologia , Magnésio/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Mutagênese Sítio-Dirigida , Oócitos/fisiologia , Canais de Potássio/biossíntese , Canais de Potássio/efeitos dos fármacos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/efeitos dos fármacos , Proteínas Recombinantes/metabolismo , Espermina/fisiologia , Relação Estrutura-Atividade , XenopusRESUMO
Distinct inward-rectifier K+ channel subunits were expressed in Xenopus oocytes and tested for their sensitivity to the channel blocker quinidine. The 'strong' inward-rectifier K+ channel IRK1 was inhibited by quinidine with an EC50 of 0.7 mM, while the 'weak' rectifier channel ROMK1 was only moderately inhibited. ROMK1(N171D)-IRK1C-term chimeric channels, which carry both sites for strong rectification of IRK1 channels (the negatively charged D171 in the second transmembrane domain and the IRK1-C-terminus including E224), displayed strong rectification like IRK1, but showed weak sensitivity to quinidine-like ROMK1, suggesting independence of quinidine binding and rectification mechanisms. Moreover, BIR10 and BIR11, two strong rectifier subunits originally cloned from rat brain, exerted subunit-specific sensitivity to quinidine, being much higher for BIR11. Quinidine blockade of IRK1 was not voltage-dependent, but strongly dependent on the pH in the superfusate. These results strongly suggest a subunit-specific interaction of inward-rectifier K+ channels with neutral quinidine within membrane lipid bilayers.
Assuntos
Encéfalo/fisiologia , Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/fisiologia , Quinidina/farmacologia , Animais , Sequência de Bases , Clonagem Molecular , Primers do DNA , Relação Dose-Resposta a Droga , Feminino , Técnicas In Vitro , Substâncias Macromoleculares , Potenciais da Membrana/efeitos dos fármacos , Dados de Sequência Molecular , Mutagênese , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Reação em Cadeia da Polimerase , Bloqueadores dos Canais de Potássio , Canais de Potássio/biossíntese , Ratos , Proteínas Recombinantes de Fusão/antagonistas & inibidores , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , XenopusRESUMO
Regulation of native inward-rectifier K+ channels by second-messenger systems is important for the control of membrane potential and excitability in various types of cell. Regulation of cloned IRK1 (Kir2.1) channels was investigated in inside-out patches from Xenopus oocytes. Fast run down of Kir2.1 channels was induced by application of GTP-gamma S to the cytoplasmic side of the patches and reversed by application of PKA plus Mg-ATP. This effect of GTP-gamma S was inhibited by the phosphatase inhibitor microcystin. These results suggest regulation of Kir2.1 channels by a G-protein-controlled phosphatase. A similar effect was observed upon application of G-protein beta gamma subunits. This effect, however, was most likely mediated by contamination of this preparation by the antioxidant DTT, since DTT by itself was also able to induce a fast run-down of Kir2.1 channels. On the other hand, DTT was found to reverse the run-down induced by GTP-gamma S, similar to PKA. This results indicate that DTT mediates two opposite effects on Kir2.1 channels. It is concluded that regulation of Kir2.1 channels is complex and most likely involves other proteins endogenously expressed in Xenopus oocytes, such as ABC-proteins, G-proteins and phosphatases, which are sensitive to oxidation.
Assuntos
Antioxidantes/farmacologia , Ditiotreitol/farmacologia , Guanosina 5'-O-(3-Tiotrifosfato)/farmacologia , Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/metabolismo , Animais , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Regulação para Baixo/efeitos dos fármacos , Interações Medicamentosas , Oócitos/metabolismo , Técnicas de Patch-Clamp , Canais de Potássio/biossíntese , Regulação para Cima/efeitos dos fármacos , XenopusRESUMO
Inward-rectifier, G-protein-regulated and ATP-dependent K+ channels form a novel gene family of related proteins which share two transmembrane segments as a common structural feature. These K+ channels are only distantly related to the voltage-gated Shaker-type K+ channels comprising six transmembrane segments. Although the quaternary structure of voltage-gated K+ channels has been extensively studied in the past, little is known about subunit assembly of inward-rectifier K+ channels. Differential sensitivity of inward-rectifier K+ channels to voltage-dependent pore block by spermine was used to analyse subunit assembly. It is shown that inward-rectifier K+ channel proteins are composed of four subunits whose assembly obeys the rules of a binomial distribution. 'Strong' and 'mild' inward-rectifier K+ channel subunits (BIR10 and ROMK1) which are co-expressed in individual auditory hair cells form hetero-tetramers. Distribution of these hetero-tetramers, however, is not binomial. Hetero- and homo-oligomeric channels form with similar probabilities resulting in independent channel populations with distinct functional properties.
Assuntos
Canais de Potássio/química , Trifosfato de Adenosina/metabolismo , Animais , Sequência de Bases , Clonagem Molecular , Primers do DNA/genética , Feminino , Proteínas de Ligação ao GTP/metabolismo , Expressão Gênica , Células Ciliadas Auditivas Externas/metabolismo , Cinética , Dados de Sequência Molecular , Oócitos/metabolismo , Reação em Cadeia da Polimerase , Canais de Potássio/genética , Canais de Potássio/metabolismo , Conformação Proteica , Ratos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , XenopusRESUMO
Single-cell reverse transcription polymerase chain reaction was carried out in three different cell types from the organ of Corti of the four-day old rat. For this purpose, pieces of the organ of Corti were mounted under a differential-interference contrast video microscope. Two different mounting configurations were used to allow imaging of cells from two almost orthogonal angles. This method afforded unequivocal recognition of various cell types in the vital tissue, and extraction of nucleus and cytoplasm of specified individual cells with a patch pipette. Messenger RNA encoding the alpha 9 acetylcholine (ACh) receptor subunit was detected and sequenced from individual outer hair cells and inner hair cells, but was not found in Deiters' cells. The identical Deiters' cells were positive for a P2x receptor subunit. This indicates cell-specific expression of the alpha 9 subunit in inner hair cells and outer hair cells and supports the hypothesis that this subunit contributes to calcium (Ca2+) permeable ionotropic ACh receptors (ACh-R). ACh-dependent Ca2+ concentration increase has been observed in both outer hair cells and inner hair cells.
Assuntos
Células Ciliadas Auditivas/metabolismo , Órgão Espiral/metabolismo , RNA Mensageiro/análise , Receptores Colinérgicos/biossíntese , Animais , Sequência de Bases , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Ratos , Ratos WistarRESUMO
ATP-sensitive potassium (K(ATP)) channels couple electrical activity to cellular metabolism via their inhibition by intracellular ATP. When examined in excised patches, ATP concentrations required for half-maximal inhibition (IC(50)) varied among tissues and were reported to be as low as 10 microM. This set up a puzzling question on how activation of K(ATP) channels can occur under physiological conditions, where the cytoplasmic concentration of ATP is much higher than that required for channel inhibition. A new twist was added to this puzzle when two recent reports showed that phospholipids such as phosphatidylinositol-4,5-bisphosphate (PIP(2)) and phosphatidyl-4-phosphate (PIP) are able to shift ATP-sensitivity of K(ATP) channels from the micro- into the millimolar range and thus provide a mechanism for physiological activation of the channels. This commentary describes how phospholipids control ATP inhibition of K(ATP) channels and how this mechanism is regulated effectively by receptor-mediated stimulation of phospholipase C.
Assuntos
Trifosfato de Adenosina/metabolismo , Proteínas de Membrana/metabolismo , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Animais , Humanos , Proteínas de Membrana/antagonistas & inibidores , Canais de Potássio , Receptores Purinérgicos P2/metabolismo , Receptores Purinérgicos P2Y2 , Fosfolipases Tipo C/metabolismoRESUMO
The biophysics of block of NMDA receptor channels has been investigated extensively during the past 8 years. In the last few years, cloned NMDA receptor channels have become available. Here we have discussed advantages and disadvantages of studying block phenomena in cloned NMDA receptors. Some recent work on the pore block of the cloned NMDA receptor channels was critically reviewed and extended by data about the calcium block. Novel effects of kainate on cloned NMDA receptors and of NMDA on cloned AMPA receptors were reported and discussed with respect to recent work concerning possible occurrence of NMDA-AMPA hybrid channels.
Assuntos
Receptores de N-Metil-D-Aspartato/química , Sequência de Aminoácidos , Animais , Clonagem Molecular , Cinética , Dados de Sequência Molecular , Mutação , Receptores de AMPA/química , Receptores de Ácido Caínico/química , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Receptores de N-Metil-D-Aspartato/genética , Proteínas Recombinantes/químicaRESUMO
Inward rectifier K+ channels of the Kir1.1 (ROMK) and Kir4.1 subtype are predominantly expressed in epithelial cells where they are responsible for K+ transport across the plasma membrane. Uniquely among the members of the Kir family, these channels are gated by intracellular pH in the physiological range. pH-gating involves structural rearrangements in cytoplasmic domains and the P-loop of the Kir protein. The energy for the gating transition is delivered by protonation of a lysine residue that is located prior to the first transmembrane segment and serves as a 'pH sensor'. The anomalous titration required for lysine operating in the neutral pH range results from its close interaction with two positively charged arginines from the distant N- and C-termini termed the R/K/R triad. Disturbance of this triad as results from a number of point mutations found in patients with hyperprostaglandin E syndrome (HPS) increases the pKa of the pH sensor and results in channels being permanently inactivated under physiological conditions. This article will focus on the mechanism of pH-gating, its implications for the tertiary structure of Kir proteins and on its significance for the pathogenesis of HPS.
Assuntos
Concentração de Íons de Hidrogênio , Ativação do Canal Iônico/fisiologia , Canais de Potássio/fisiologia , Potássio/metabolismo , Animais , Cátions/metabolismo , Cloretos/metabolismo , Clonagem Molecular , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Humanos , Recém-Nascido , Líquido Intracelular/química , Ativação do Canal Iônico/efeitos dos fármacos , Transporte de Íons/efeitos dos fármacos , Rim/metabolismo , Erros Inatos do Metabolismo/genética , Erros Inatos do Metabolismo/metabolismo , Modelos Moleculares , Mutação Puntual , Canais de Potássio/química , Canais de Potássio/efeitos dos fármacos , Canais de Potássio/genética , Conformação Proteica , Proteínas Recombinantes de Fusão/metabolismo , Relação Estrutura-Atividade , Reagentes de Sulfidrila/farmacologia , Síndrome , XenopusRESUMO
The KATP channel is a heterooctamer composed of two different subunits, four inwardly rectifying K+ channel subunits, either Kir6. 1 or Kir6.2, and four sulfonylurea receptors (SUR), which belong to the family of ABC transporters. This unusual molecular architecture is related to the complex gating behaviour of these channels. Intracellular ATP inhibits KATP channels by binding to the Kir6.x subunits, whereas Mg-ADP increases channel activity by a hydrolysis reaction at the SUR. This ATP/ADP dependence allows KATP channels to link metabolism to excitability, which is important for many physiological functions, such as insulin secretion and cell protection during periods of ischemic stress. Recent work has uncovered a new class of regulatory molecules for KATP channel gating. Membrane phospholipids such as phosphoinositol 4, 5-bisphosphate and phosphatidylinositiol 4-monophosphate were found to interact with KATP channels resulting in increased open probability and markedly reduced ATP sensitivity. The membrane concentration of these phospholipids is regulated by a set of enzymes comprising phospholipases, phospholipid phosphatases and phospholipid kinases providing a possible mechanism for control of cell excitability through signal transduction pathways that modulate activity of these enzymes. This review discusses the mechanisms and molecular determinants that underlie gating of KATP channel by nucleotides and phospholipids and their physiological implications.
Assuntos
Difosfato de Adenosina/farmacologia , Trifosfato de Adenosina/fisiologia , Ativação do Canal Iônico/fisiologia , Fosfolipídeos/fisiologia , Canais de Potássio/fisiologia , Potássio/metabolismo , Difosfato de Adenosina/fisiologia , Trifosfato de Adenosina/farmacologia , Animais , Proteínas de Ligação ao GTP/fisiologia , Guanosina Trifosfato/fisiologia , Humanos , Hidrólise , Líquido Intracelular/metabolismo , Ativação do Canal Iônico/efeitos dos fármacos , Transporte de Íons/efeitos dos fármacos , Magnésio/farmacologia , Magnésio/fisiologia , Fosfatidilinositóis/farmacologia , Fosfatidilinositóis/fisiologia , Fosfolipases/fisiologia , Fosfolipídeos/farmacologia , Monoéster Fosfórico Hidrolases/fisiologia , Fosfotransferases/fisiologia , Canais de Potássio/efeitos dos fármacos , Transdução de SinaisRESUMO
1. The non-linear capacitance (Cnon-lin) of postnatal outer hair cells (OHCs) of the rat was measured by a patch-clamp lock-in technique. Cnon-lin is thought to result from a membrane protein that provides the molecular basis for the unique electromotility of OHCs by undergoing conformational changes in response to changes in membrane potential (Vm). Protein conformation is coupled to Vm by a charged voltage sensor, which imposes Cnon-lin on the OHC. Cnon-lin was investigated in order to characterize the surface expression and voltage dependence of this motor protein during postnatal development. 2. On the day of birth (P0), Cnon-lin was not detected in OHCs of the basal turn of the cochlea, whilst it was 89 fF in apical OHCs. Cnon-lin increased gradually during postnatal development and reached 2.3 pF (basal turn, P9) and 7.5 pF (apical turn, P14) at the oldest developmental stages covered by our measurements. The density of the protein in the plasma membrane, deduced from non-linear charge movement per membrane area, increased steeply between P6 and P11 and reached steady state (4200 e- microm-2) at about P12. 3. Voltage at peak capacitance (V) shifted with development from hyperpolarized potentials shortly after birth (-88.3 mV, P2) to the depolarized potential characteristic of mature OHCs (-40.8 mV, P14). This developmental difference in V was also observed in outside-out patches immediately after patch excision. During subsequent wash-out V shifted towards the depolarized value found in the adult state, suggesting a direct modulation of the molecular motor. 4. Thus, the density of the motor protein in the plasma membrane and also its voltage dependence change concomitantly in the postnatal period and reach adult characteristics right at the onset of hearing.
Assuntos
Células Ciliadas Auditivas Externas/crescimento & desenvolvimento , Proteínas Musculares/fisiologia , Animais , Condutividade Elétrica , Eletrofisiologia , Cobaias , Potenciais da Membrana , Ratos , Ratos WistarRESUMO
Inward-rectifier potassium (Kir) channels comprise a superfamily of potassium (K+) channels with unique structural and functional properties. Expressed in virtually all types of cells they are responsible for setting the resting membrane potential, controlling the excitation threshold and secreting K+ ions. All Kir channels present an inwardly rectifying current-voltage relation, meaning that at any given driving force the inward flow of K+ ions exceeds the outward flow for the opposite driving force. This inward-rectification is due to a voltage-dependent block of the channel pore by intracellular polyamines and magnesium. The present molecular-biophysical understanding of inward-rectification and its physiological consequences is the topic of this review. In addition to polyamines, Kir channels are gated by intracellular protons, G-proteins, ATP and phospholipids depending on the respective Kir subfamily as detailed in the following review articles.
Assuntos
Ativação do Canal Iônico/efeitos dos fármacos , Poliaminas/farmacologia , Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/efeitos dos fármacos , Potássio/metabolismo , Animais , Clonagem Molecular , Humanos , Transporte de Íons/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Modelos Moleculares , Canais de Potássio/classificação , Canais de Potássio/fisiologia , Conformação Proteica , Proteínas Recombinantes de Fusão/efeitos dos fármacos , Proteínas Recombinantes de Fusão/metabolismoRESUMO
The inactivation of the sodium channels in human medulloblastoma cells was investigated with the whole-cell recording technique. The potential dependence of inactivation ("inactivation curve") was determined by imposing a series of prepulses of varying amplitude on the membrane potential and measuring the maximum sodium current flowing after each prepulse at the test potential of -20 mV. The time dependence of inactivation was investigated by determining inactivation curves with prepulses of variable duration. A prolongation of the prepulse increased the degree of inactivation, even when the prepulse duration was much greater than the time constant for fast inactivation. This is explained by the existence of two additional states of "intermediate" inactivation of the sodium channel, the transition to which is slower than that to the state of fast inactivation and faster than that to the state of slow inactivation. The antiarrhythmic drug tocainide had no effect on fast inactivation, but a strong effect on intermediate inactivation. This explains the use dependence of this drug. The reaction model given by Chiu (1977) for the transitions from the open into the closed state of inactivation and vice versa is extended.
Assuntos
Neoplasias Cerebelares/metabolismo , Lidocaína/análogos & derivados , Meduloblastoma/metabolismo , Canais de Sódio/fisiologia , Humanos , Lidocaína/farmacologia , Modelos Biológicos , Canais de Sódio/efeitos dos fármacos , Tetrodotoxina/farmacologia , Tocainide , Células Tumorais CultivadasRESUMO
The auxiliary beta-subunit KCNMB2 (beta(2)) endows the non-inactivating large conductance Ca(2+)- and voltage-dependent potassium (BK) channel with fast inactivation. This process is mediated by the N terminus of KCNMB2 and closely resembles the "ball-and-chain"-type inactivation observed in voltage-gated potassium channels. Here we investigated the solution structure and function of the KCNMB2 N terminus (amino acids 1-45, BKbeta(2)N) using NMR spectroscopy and patch clamp recordings. BKbeta(2)N completely inactivated BK channels when applied to the cytoplasmic side; its interaction with the BK alpha-subunit is characterized by a particularly slow dissociation rate and an affinity in the upper nanomolar range. The BKbeta(2)N structure comprises two domains connected by a flexible linker: the pore-blocking "ball domain" (formed by residues 1-17) and the "chain domain" (between residues 20-45) linking it to the membrane segment of KCNMB2. The ball domain is made up of a flexible N terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C terminus. These structural properties explain the functional characteristics of BKbeta(2)N-mediated inactivation.
Assuntos
Canais de Potássio Cálcio-Ativados , Canais de Potássio/química , Sequência de Aminoácidos , Animais , Feminino , Subunidades alfa do Canal de Potássio Ativado por Cálcio de Condutância Alta , Subunidades beta do Canal de Potássio Ativado por Cálcio de Condutância Alta , Canais de Potássio Ativados por Cálcio de Condutância Alta , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Canais de Potássio/fisiologia , Estrutura Secundária de Proteína , Subunidades Proteicas , XenopusRESUMO
The inhibitory effect of short antisense oligodeoxynucleotides (aODNs) on cRNA expression in Xenopus oocytes was measured using an electrophysiological assay based on subunit-specific block of cloned alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate receptors. The effect of both phosphorothioate-modified (PS) and phosphodiester (PO) aODNs was strongly length dependent with a half-maximal inhibition calculated for an oligo length of 7.6 nucleotides (nt) and 9.9 nt, respectively. More than 95% inhibition was mediated by a PS aODN of 12 nt and by PO aODNs > or = 15 nt. At a given length PS and PO aODNs showed differential dependence of their inhibitory effect on the injected aODN concentration (half-maximal inhibition at 18 ng/microliter for a PO 12-mer and at 0.19 ng/microliter for a PS 12-mer) and differential saturation behavior. The inhibitory effect of aODNs, even as short as 8 nt for PS oligomers, was highly sequence specific, but almost independent of the position of the respective target site on the cRNA (for PS 8-mers, > or = 70% expression inhibition throughout the tested target sites from the translation initiation to the 3'-untranslated region). Thus, short PS aODNs can be reliably used in order to specifically inhibit protein expression in experiments addressing physiological, molecular biological, and perhaps even therapeutical issues.