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Herein, we represent a bimetallic approach to enhance the defect number, leading to eight defect sites per node in a metal-organic framework, showing both a higher SO2 adsorption capacity and higher SO2/CO2 selectivity. The results can be further strongly supported by density functional theory calculations.
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Sperm-associated antigen 1 (SPAG1) is considered to be associated with infertility and tumorigenesis. However, its function in acute myeloid leukemia (AML) remains unclear. In this study, we evaluated the expression level of SPAG1 and explored its clinical prognostic value in patients with AML, as well as its biological function in AML cells. SPAG1 is widely expressed in AML patients, resulting in a poor prognosis. However, its expression was not associated with Fms-related receptor tyrosine kinase 3 (FLT3) mutations. Utilizing the RNA interference knockdown tests, we found that SPAG1 could promote the proliferation and survival of AML cells and regulate the expression of structural maintenance of chromosomes protein 3 (SMC3), activating the ERK/MAPK signaling pathway. Furthermore, we discovered that inhibiting SPAG1 impacted AML cell susceptibility to venetoclax. In conclusion, SPAG1 may serve as a potential therapeutic target in AML.
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Antígenos de Superfície , Compostos Bicíclicos Heterocíclicos com Pontes , Proteínas de Ligação ao GTP , Leucemia Mieloide Aguda , Sulfonamidas , Antígenos de Superfície/uso terapêutico , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Proteínas de Ligação ao GTP/genética , Proteínas de Ligação ao GTP/metabolismo , Humanos , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/genética , Mutação , Proteínas Tirosina Quinases/metabolismo , Transdução de Sinais , Sulfonamidas/farmacologia , Tirosina Quinase 3 Semelhante a fms/genética , Tirosina Quinase 3 Semelhante a fms/metabolismoRESUMO
Constructing robust adsorbents for SO2 removal remains a challenging issue. Herein, a U-Co bimetallic-organic framework, namely, ECUT-123, showing a helical 1D pore decorated by abundant -CH3 groups, enables ultrahigh stability under acid, base, and water. This merit further supports its big potential for the removal of trace SO2.
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Herein, we report a highly rare robust 4d-5f bimetal-organic framework that shows high porosity and thermal/chemical stability and thus is capable of removing trace SO2 from a SO2/CO2/N2 mixture even under humid conditions. This work not only shows a novel adsorbent for SO2 removal but also extends the function of actinium-based coordination compounds.
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Removal of trace SO2 from an SO2-containing product is now receiving increasing attention. However, designing a robust porous adsorbent with high SO2 adsorption capacity and good SO2/CO2 selectivity, as well as validity under humid conditions, is still a challenging task. Herein, we report a porous cage-based metal-organic framework, namely ECUT-111, which contains two distinct cages with apertures of 5.4 and 10.2 Å, respectively, and shows high a BET of up to 1493 m2/g and a pore volume of 0.629 cm3/g. Impressively, ECUT-111 enables an ultrahigh SO2 uptake of up to 11.56 mmol/g, exceeding most reported top-performing adsorbents for such a use. More importantly, complete separation of trace SO2 from SO2/CO2 and SO2/CO2/N2 mixtures, especially under humid conditions, and excellent recycle use were observed for ECUT-111, suggesting its superior application in desulfurization of SO2-containing products.
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Encapsulating a certain guest molecule in an assigned molecular compartment and then endowing the corresponding potential remains a huge challenge for metal-organic frameworks. To this end, we demonstrate a good example, for the first time, based on an actinide-based MOF. The used MOF (namely, ECUT-300) shows a unique uranyl-TPE anionic skeleton with three distinct cages, viz., mesopore A (2.8 nm), mesopore B (2.0 nm), and micropore C (0.9 nm). Through solid-liquid reaction, a RhB+ molecule can be encapsulated into ECUT-300 with the exact location in mesopore B, whereas the encapsulation of a metal-organic cation of [Fe(tpy)2]3+ was observed with the location in micropore C, suggesting unprecedented classified encapsulation. Impressively, the potential of the resulting guest@MOF composites is also highly dependent on the type of encapsulated guest molecules, for example, white-light emission for RhB+ and selective adsorption of C2H2 over CO2 for [Fe(tpy)2]3+.
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Herein, we report a new metal-organic framework (MOF), namely, ECUT-77, which is built on rod-shaped secondary building units, showing a high Brunauer-Emmett-Teller surface area of 760.3 cm2/g, a pore volume of 0.4 cm3/g, and an aperture of about 1 nm. This MOF enables both high SO2 adsorption capacity up to 8.0 mmol/g at 0.92 bar and room temperature and a high SO2/CO2 selectivity of 44, resulting in excellent SO2 separation upon a ECUT-77 column from a SO2/CO2 mixture containing 2000 ppm of SO2.
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Enriched environment (EE) with a complex combination of sensorimotor, cognitive and social stimulations has been shown to enhance brain plasticity and improve recovery of functions in animal models of stroke. The present study extended these findings by assessing whether the three-phase EE intervention paradigm would improve neurovascular remodeling following ischemic stroke. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO). A three-phase EE intervention paradigm was designed in terms of the different periods of cerebral ischemia by periodically rearranging the EE cage. Morris water maze (MWM) tests were performed to evaluate the learning and memory function. Multimodal MRI was applied to examine alterations to brain structures, intracranial vessels, and cerebral perfusion on the 31st day after MCAO. The changes of capillaries ultrastructure were examined by transmission electron microscope. Double-immunofluorescent staining was used to evaluate neurogenesis and angiogenesis. The expression of angiogenesis-related factors and neurovascular remodeling related signaling pathways including Phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/glycogen synthase kinase-3 (GSK-3)/ß-catenin and the axon guidance molecules were detected by Western blot analysis. MRI measurements revealed that EE treatment significantly increased survival volume of cortex and striatum, improved cerebral blood flow (CBF), amplified anterior azygos cerebral artery (azACA), ipsilateral internal carotid artery (ICA) and anterior communicating artery (AComA) vessel signal compared with standard housed rats (IS). Consistent with these findings, EE reduced ischemic BBB damage of capillary, enhanced endogenous angiogenesis and modified the expression of VEGF, Ang-1 or Ang-2 in ischemic rats. Additionally, this proangiogenic effect was consistent with the increased progenitor cell proliferation and neuronal differentiation in the peri-infarct cortex and striatum after EE intervention. Specifically, EE intervention paradigm markedly increased expression of phosphorylated PI3K, AKT and GSK-3, but reduced phosphorylated ß-catenin. Moreover, the axon guidance proteins expression level was significant higher in EE group. In parallel to these findings, EE significantly enhanced recovery of lost spatial learning memory function in MCAO rats without affecting infarct size. Together, MRI findings along with histological results strongly supported that the three-phase EE paradigm benefited neurovascular reorganization and thereby improved poststroke cognitive function. Moreover, our findings suggest that this type of EE paradigm induced neurogenesis and angiogenesis, at least in part, via regulating PI3K/AKT/GSK-3/ß-catenin signaling pathway and activation of the intrinsic axonal guidance molecules in animal models of ischemic stroke.
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Isquemia Encefálica/fisiopatologia , Encéfalo/fisiopatologia , Meio Ambiente , AVC Isquêmico/fisiopatologia , Recuperação de Função Fisiológica/fisiologia , Animais , Encéfalo/patologia , Circulação Cerebrovascular/fisiologia , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/patologia , Masculino , Neurogênese/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Ratos Sprague-Dawley , Acidente Vascular Cerebral/patologia , Acidente Vascular Cerebral/fisiopatologiaRESUMO
We report for the first time the construction of mechanoresponsive and redox-active metal-organic frameworks (MOFs) and covalent organic frameworks (COFs) by anchoring ferrocene (Fc) pendants as mechanophores in the pore wall. This work outlines a simple, general, and low-cost route to tailor MOFs and COFs by a Fc unit for mechanoresponsive nature, the release of Fe ions, redox behavior, and modulation of the skeleton charge together.
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To acquire the eggplant transcriptome under high temperature stress, 18 cDNA libraries were constructed and sequenced. A total of 136.31 Gb of clean data was obtained, and 88.86%-92.35% of the clean reads were mapped to the eggplant reference genome. Under high temperature, the number of down-regulated genes was more than that of up-regulated genes and there were more differentially expressed genes on the 10th day after flowering than on the 15th and 20th days after flowering. On the 10th day after flowering, the key genes CHI, 3GT, F3'5'H, DFR2, ANS, and F3H in anthocyanin synthetic pathway and most ERF, WRKY, bHLH, and MYB transcription factors were all down-regulated. High temperature significantly decreased the total anthocyanin content in peels. The results showed that at the early stage of peel coloring, high temperature inhibited the expressions of key genes in anthocyanin biosynthetic pathways through the regulation of transcription factors, leading to a significant decrease in total anthocyanin content, which might reduce the peel color in eggplant.
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Temperatura Alta , Pigmentação/genética , Solanum melongena/genética , Transcriptoma , Antocianinas/biossíntese , Frutas , Regulação da Expressão Gênica de Plantas , Biblioteca Gênica , Análise de Sequência de RNA , Fatores de Transcrição/genéticaRESUMO
Transition-metal-organic frameworks (MOFs) have been regarded as one of the most intriguing electrocatalysts because of its low cost and diversity in functional organic groups and metal centers. Different from the common strategies of tuning the ratio of metal centers in multivariate MOFs, here, ultralow-content Fe2O3 is decorated on the surface of monometallic Ni-MOF-74 based on the fast "phenol-iron (Fe)" surface reaction between Fe2+ and the surface hydroxyl group in Ni-MOF-74. Benefiting from this flexible method, the Fe loading can be finely modulated and thus a series of Fe-decorated Ni-MOF-74 with different Fe contents are prepared. The optimized 0.6 wt % Fe2O3@Ni-MOF-74 with the Fe loading of 0.6 wt % only needs the overpotential of 264 mV to deliver 10 mA cm-2, which obviously outperforms Fe-free Ni-MOF-74 (323 mV) and other Fe2O3@Ni-MOF-74 and is even superior to the commercial IrO2 benchmark (300 mV). X-ray photoelectron spectroscopy results disclose that Fe decoration can obviously modulate the electronic structure of Ni center in Ni-MOF-74, thereby resulting in enhanced oxygen evolution reaction activity. This work opens up a new avenue to fabricate excellent MOF-based electrocatalysts for direct utilization in an electrocatalytic process.
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The purpose of this article is to identify Daphne genkwa and its adulterants, Wikstroemia chamaedaphne, according to the morphological and microstructure characteristics of their stem and foliage. The root of D.genkwa was studied simultaneously. The results indicated that the crude drug and processed pieces of Genkwa Ramulus were mainly composed of stems and branches where obvious opposite petiole scars and branch marks were able to be seen on their nodes. Otherwise, foliage or peduncles generally couldn't be found. Moreover, the fine silver flocculent fibers could be observed in the bark of fracture surface. The adulterants were the plant segments which were composed of stems, foliage and peduncles with spikelet-pedicel scars. There existed microstructures differences between Genkwa Ramulus and its adulterants. In the former, single thick lignified phloem fibers were interspersed in the stem phloem of the transverse section with very thick wall and unicellular non-glandular hairs could be observed on the lower epidermis of foliage. Nevertheless, in the latter, there was no thick lignified phloem fibers in cross section of stem phloem, the outer wall of epidermal cells of foliage hadthick cuticles and no non-glandular hairs in lower epidermis of foliage. The results can be used for the identification and the quality standard of the crude drug and processed pieces of D.genkwa.The characteristics of the microstructures and the transverse section can be used to identify the radix D.genkwa.
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Daphne/anatomia & histologia , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/normas , Wikstroemia/anatomia & histologia , Microscopia , Plantas Medicinais/anatomia & histologiaRESUMO
A significant synergic effect between a metal-organic framework (MOF) and Fe2 SO4 , the so-called MOF+ technique, is exploited for the first time to remove toxic chromate from aqueous solutions. The results show that relative to the pristine MOF samples (no detectable chromate removal), the MOF+ method enables super performance, giving a 796â Crâ mg g-1 adsorption capacity. The value is almost eight-fold higher than the best value of established MOF adsorbents, and the highest value of all reported porous adsorbents for such use. The adsorption mechanism, unlike the anion-exchange process that dominates chromate removal in all other MOF adsorbents, as unveiled by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), is due to the surface formation of Fe0.75 Cr0.25 (OH)3 nanospheres on the MOF samples.
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A dual temperature- and light-responsive C2 H2 /C2 H4 separation switch in a diarylethene metal-organic framework (MOF) is presented. At 195â K and 100â kPa this MOF shows ultrahigh C2 H2 /C2 H4 selectivity of 47.1, which is almost 21.4 times larger than the corresponding value of 2.2 at 293â K and 100â kPa, or 15.7 times larger than the value of 3.0 for the material under UV at 195â K and 100â kPa. The origin of this unique control in C2 H2 /C2 H4 selectivity, as unveiled by density functional calculations, is due to a guest discriminatory gate-opening effect from the diarylethene unit.
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Herein, we report a robust azo-metal-organic framework (MOF), namely, ECUT-15, which can be described as a 10-connected bct net built on trinuclear Co3 subunits. The activated samples of it perform a somewhat breathing behavior. Most importantly, under UV irradiation, this MOF performs outstanding photoswitching behavior toward CO2, giving great variation in the CO2 capture/release performance, for example, 45% under static conditions and 75% under dynamic measurements, as well as instantaneous release of up to 78%.
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Microscopic identification and NIRS methods were applied to identify Clematidis Radix et Rhizoma of two different origins. The results showed that both methods could identify the samples. NIRS could identify the two samples nondestructively, and provides a basis for establishment of a standard herbs radix clematidis NIRS fingerprint in the future.
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Clematis/química , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/química , Rizoma/química , Espectroscopia de Luz Próxima ao Infravermelho/métodos , China , Clematis/classificaçãoRESUMO
To establish quality standards of Euonymus fortunei, and supply scientific evidence for the quality control of Euonymus fortunei. Empirical and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water, total ash, acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmaco- poeia (2010). Dulcitol and reference herbs were used to identify materia medica of Euonymus fortunei by TLC method. The total flavonol glycosides contents were analysed by HPLC method, using quercetin and kaempferol as reference substances. Quercetin and kaempferol were separated on a C18 column (4.6 mm x 250 mm, 5 µm) with methanol-0.1% formic acid(51:49) as the mobile phase and detected at 366 nm. The flavonoid aglycones content was then multiplied by a conversion coefficient, and the result was the total flavonol glycosides content. The macroscopical identification, microscopic features and TLC methods were proper. The average contents of water, total ash, acid-insoluble ash, alcohol-soluble extractive and total flavonol glycosides were 8.76%, 6.48%, 0.31%, 17.48% and 0.211% , respectively. The quality standards established on the basis of the research results were suitable for the quality evaluation of Euonymus fortunei.
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Medicamentos de Ervas Chinesas/química , Euonymus/química , China , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Medicamentos de Ervas Chinesas/normas , Euonymus/anatomia & histologia , Espectrometria de Massas , Controle de QualidadeRESUMO
HCC, also known as hepatocellular carcinoma, is a frequently occurring form of cancer with an unfavorable prognosis. This research constructed a prognostic signature related to ubiquitination and investigated its correlation with the response to immunotherapy in HCC. The Molecular Signatures Database provided a compilation of genes associated with ubiquitination. A gene signature related to ubiquitination was obtained through Cox regression using the Least Absolute Shrinkage and Selection Operator method. The genetic factors CPY26B1, MCM10, SPINK4, and TRIM54 notably impacted the outcomes of HCC. The patients were divided into two groups: one group had a high risk of poor survival while the other had a low risk but a greater chance of controlling HCC progression. Both univariate and multivariate analyses using Cox regression found the risk score to be an independent predictor of HCC prognosis. Gene set enrichment analysis (GSEA) indicated enrichment in cell cycle and cancer-related microRNAs in high-risk groups. The tumor microenvironment (TME), response to immunotherapy, and effectiveness of chemotherapy medications positively correlated with the risk score. In the high-risk group, erlotinib showed higher IC50 values compared to the low-risk group which exhibited higher IC50 values for VX-11e, AKT inhibitor VIII, AT-7519, BMS345541, Bortezomib, CP466722, FMK, and JNK-9L. The results of RT-qPCR revealed that the expression of four UEGs was higher in tumor tissue as compared to normal tissue. Based on the genes that were expressed differently and associated with ubiquitination-related tumor categorization, we have developed a pattern of four genes and a strong nomogram that can predict the prognosis of HCC, which could be useful in identifying and managing HCC.
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Carcinoma Hepatocelular , Imunoterapia , Neoplasias Hepáticas , Ubiquitinação , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/terapia , Ubiquitinação/genética , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/terapia , Prognóstico , Regulação Neoplásica da Expressão Gênica , Microambiente Tumoral/genética , Microambiente Tumoral/imunologia , Masculino , Feminino , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , TranscriptomaRESUMO
2,3,5,6-Tetramethylpyrazine (TMP) as an active ingredient extracted from a traditional Chinese herbal medicine Ligusticum chuanxiong Hort. has been proved to penetrate blood-brain barrier (BBB) and show neuroprotective effects on cerebral ischemia. However, whether TMP could regulate astrocytic reactivity to facilitate neurovascular restoration in the subacute ischemic stroke needs to be urgently verified. In this research, permanent occlusion of the middle cerebral artery (MCAO) model was conducted and TMP (10, 20, 40 mg/kg) was intraperitoneally administrated to rats once daily for 2 weeks. Neurological function was evaluated by motor deficit score (MDS). Magnetic resonance imaging (MRI) was implemented to analyze tissue injury and cerebral blood flow (CBF). Magnetic resonance angiography (MRA) was applied to exhibit vascular signals. Transmission electron microscopy (TEM) was performed to detect the neurovascular unit (NVU) ultrastructure. Haematoxylin and eosin (HE) staining was utilized to evaluate cerebral histopathological lesions. The neurogenesis, angiogenesis, A1/A2 reactivity, aquaporin 4 (AQP4) and connexin 43 (Cx43) of astrocytes were observed with immunofluorescent staining. Then FGF2/PI3K/AKT signals were measured by western blot. Findings revealed TMP ameliorated neurological functional recovery, preserved NVU integrity, and enhanced endogenous neurogenesis and angiogenesis of rats with subacute ischemia. Shifting A1 to A2 reactivity, suppressing excessive AQP4 and Cx43 expression of astrocytes, and activating FGF2/PI3K/AKT pathway might be potential mechanisms of promoting neurovascular restoration with TMP after ischemic stroke.
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Ischemic stroke elicits white matter injury typically signed by axonal disintegration and demyelination; thus, the development of white matter reorganization is needed. 2,3,5,6-Tetramethylpyrazine (TMP) is widely used to treat ischemic stroke. This study was aimed to investigate whether TMP could protect the white matter and promote axonal repair after cerebral ischemia. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO) and treated with TMP (10, 20, 40 mg/kg) intraperitoneally for 14 days. The motor function related to gait was evaluated by the gait analysis system. Multiparametric magnetic resonance imaging (MRI) was conducted to noninvasively identify gray-white matter structural integrity, axonal reorganization, and cerebral blood flow (CBF), followed by histological analysis. The expressions of axonal growth-associated protein 43 (GAP-43), synaptophysin (SYN), axonal growth-inhibitory signals, and guidance factors were measured by Western blot. Our results showed TMP reduced infarct volume, relieved gray-white matter damage, promoted axonal remodeling, and restored CBF along the peri-infarct cortex, external capsule, and internal capsule. These MRI findings were confirmed by histopathological data. Moreover, motor function, especially gait impairment, was improved by TMP treatment. Notably, TMP upregulated GAP-43 and SYN and enhanced axonal guidance cues such as Netrin-1/DCC and Slit-2/Robo-1 but downregulated intrinsic growth-inhibitory signals NogoA/NgR/RhoA/ROCK-2. Taken together, our data indicated that TMP facilitated poststroke axonal remodeling and motor functional recovery. Moreover, our findings suggested that TMP restored local CBF, augmented guidance cues, and restrained intrinsic growth-inhibitory signals, all of which might improve the intracerebral microenvironment of ischemic areas and then benefit white matter remodeling.