RESUMO
The total protein content of CSF collected from the cisterna magna (cisternal fluid) of 98 patients (58 males and 40 females) with no neurological diseases was determined by the colorimetric method devised by Lowry and co-workers. This method has been previously chosen based mainly on it's sensitivity, accuracy and low fluid consumption. The CSF samples were normal with regard to pressure, color, citology and it's content in glucose, chloride and urea. Complement fixation tests for syphilis and cisticercosis, as well as the globulin tests (Pandy, Nonne--Appelt and Takata-Ara) were negative. The average value and the normal range of cisternal fluid total protein was calculated for the mixed population (males and females). The mean protein value was 26.78 mg/100 ml, the lower and upper limits were respectively 13.20 and 40.36 mg/100 ml. These data are higher than those stated in some publications, and factors that could interfere in the different results mentioned in the literature are briefly discussed. Since the statistical analysis of the results showed that the mean total protein concentration in males (28.76 mg/100 ml) was higher than in females (23.91 mg/100 ml), normal limits for each sex were established: 16.96 to 47.13 mg/100 ml for males and 14.76 to 42.76 mg/100 ml for females.
Assuntos
Proteínas do Líquido Cefalorraquidiano/análise , Cisterna Magna/análise , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Punções , Fatores SexuaisAssuntos
Doença de Chagas/imunologia , Enzimas Ativadoras do Complemento/análise , Convertases de Complemento C3-C5/análise , Complemento C3/análise , Animais , Complexo Antígeno-Anticorpo , Ativação do Complemento , Complemento C3/metabolismo , Convertases de Complemento C3-C5/metabolismo , Imunodifusão , Imunoeletroforese , Camundongos , Trypanosoma cruziAssuntos
Anticorpos/análise , Formação de Anticorpos/efeitos dos fármacos , Eosinófilos/efeitos dos fármacos , Etilenodiaminas/uso terapêutico , Niridazol/uso terapêutico , Esquistossomose/tratamento farmacológico , Xantenos/uso terapêutico , Adolescente , Adulto , Criança , Estudos de Avaliação como Assunto , Imunofluorescência , Testes de Hemaglutinação , Humanos , Imunodifusão , Testes de Precipitina , Schistosoma mansoni , Esquistossomose/imunologiaRESUMO
Fourteen patients with lobomycosis from the Amazon Basin were studied in order to assess their humoral and cellular immunological responses. The humoral response was normal, as shown by the normal levels of serum immunoglovulins. The cellular immunity was depressed for recent sensitizations as measured by skin contact test with DNCB (negative in 83.3% of 12 patients). The intradermal reactions to PPD and fungal extracts suggested that all patients were able to respond to at least 2 of the antigens used. It was also observed that patients reacting to PPD did not become sensitized to DNCB. Conversely, some Mantoux negative patients had positive reactions to DNCB. Both tests were negative in 3 patients.