[The results of monitoring of antigen types of rotaviruses of group A on the territory of the Russian Federation in 2011-2015.]

The data of seasonal monitoring are presented concerning antigen types of rotaviruses group A circulating on the territory of the Russian Federation in the periods of seasonal uprising of morbidity in 2011-2015. Annually, the study included from 10 to 12 subjects of the Russian Federation with annual testing from 444 to 728 samples from children aged younger than 5 years with acute infection diarrhea. In the seasons of 2011-2012, 2012-2013, 2013-2014, 2014-2015 the most prevalent [P] G types of rotaviruses correspondingly made up to: G4[P]8 -50.2%-36.5%-43.8%-1.6%; G1[P]8 - 26.6%-14.3%-27.3%-22.5%; G3[P]8 - 4.4%-23.7%-4.2%-2.0%; G9[P]8 - 4.3%-5.3%-10.1%-7.1%; G2[P]4 - 7.7%-7.9%-9.0%-3.1%. The expressed territorial irregularity of prevalence of antigen types of retroviruses was observed.

[Collective immunity against poliomyelitis among the population of several regions of Russia].

The goal of this work was to estimate the collective immunity against poliomyelitis among the population of 8 regions and republics of Russia. The rates of the collective immunity against poliomyelitis allow the polio vaccination quality to be estimated and the population protection rate to be simultaneously demonstrated. A total of 8 regions (2138 people) were tested. The antibodies to the polioviruses of 1-3 types were determined against the vaccine Sabin strains in the neutralization test in the RD cell line. As a result, we found that vaccination against poliomyelitis in all observed regions was maintained at the required high level. Thus, the number of people with antibodies to the polio in most regions and age groups approximates or reaches 100%, while GMT is also high. This work demonstrated the necessity of the continuation of vaccination against poliomyelitis and control over collective immunity.

[ERIAD mass spectrometry (electrospray with controlled fragmentation) is the common method for metallomics and biochemistry of elementoorganic molecules].

The physical aspects of the ERIAD method (electrospray with controlled fragmentation) in terms of the problems of metallomics and biochemistry of elementoorganic compounds have been considered. It was shown that the method is well suited to study the objects of this class since it enables one to change from the molecular to the elemental analysis merely by changing the voltage between the nozzle and the skimmer. In the regime of molecular analysis, it is possible to determine the mass of the molecule as a whole and decipher its structure by controlled fragmentation, and in the regime of elemental analysis the number of atoms of heteroelements incorporated in the molecule can be quantified.

Electrospray mass spectrometry with controlled in-source atomization (ERIAD) as a promising elemental method: evaluation of analytical features.

Electrospray ionization with controlled in-source fragmentation and atomization (ERIAD) is promising method for mass-spectrometric elemental analysis. This paper concerns the main analytical features and necessary instrumentation for ERIAD. The method is shown to be effective for more than 70 elements of the periodic table: those that present as cations in a water solution. It shows a high absolute sensitivity with a low spread from Li to U, and a detection limit of tens of parts per trillion. The soft ionization used results in the absence of plasma chemical reaction products, typical for inductively coupled plasma mass spectrometry, and the spectra are nearly free from interferences. The method does not require a high resolution and can be used for samples with an artificial isotopic content. The method is essentially valid for radiochemistry and metallomics, as it has the possibility to combine measurements of chemical, elemental, and even isotopic content simultaneously.