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1.
J Environ Manage ; 361: 121224, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38810462

RESUMO

In the context of China's dual carbon target, reducing personal carbon emissions has been identified as a crucial strategy to achieve the target. The 2022 Digital China Development Report emphasizes the significance of implementing the Carbon Generalized System of Preferences (CGSP) in driving individual carbon reduction efforts in China. However, the psychological factors influencing public participation in CGSP are still unknown. Based on the Extended Theory of Planned Behavior (TPB), this study explored the psychological factors of different personality trait groups' participation in the CGSP and categorized 712 respondents into Compatible, Positive, Responsible, and Susceptible based on the MINI-IPIP scale and the K-means method. The results show that the strength of willingness to engage (WTE) in CGSP was ranked as: Compatible > Positive > Responsible > Susceptible and the WTE of compatible groups is more influenced by attitude, while Perceived Behavioral Control (PBC) plays a more crucial role in other groups. Personal Norms (PN) and Policy Awareness (PA) were significant for all specific personality groups except the Susceptible group. Surprisingly subjective norms had little to do with WTE. We believe that policymakers should consider the impact of PBC on WTE when formulating policies and raise the expectation of residents in terms of the value they can obtain from participating in CGSP. Additionally, promotional activities related to PN and PA in connection with CGSP should be conducted. These efforts may help individuals better engage in CGSP.


Assuntos
Personalidade , Humanos , China , Atitude , Carbono , Teoria Psicológica , Teoria do Comportamento Planejado
2.
Water Sci Technol ; 89(8): 2164-2176, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38678416

RESUMO

To assess the viability and effectiveness of bioretention cell in enhancing rainwater resource utilization within sponge cities, this study employs field monitoring, laboratory testing, and statistical analysis to evaluate the water purification capabilities of bioretention cell. Findings indicate a marked purification impact on surface runoff, with removal efficiencies of 59.81% for suspended solids (SS), 39.01% for chemical oxygen demand (COD), 37.53% for ammonia nitrogen (NH3-N), and 30.49% for total phosphorus (TP). The treated water largely complies with rainwater reuse guidelines and tertiary sewage discharge standards. Notably, while previous research in China has emphasized water volume control in sponge city infrastructures, less attention has been given to the qualitative aspects and field-based evaluations. This research not only fills that gap but also offers valuable insights and practical implications for bioretention cell integration into sponge city development. Moreover, the methodology and outcomes of this study serve as a benchmark for future sponge city project assessments, offering guidance to relevant authorities.


Assuntos
Cidades , Análise da Demanda Biológica de Oxigênio , China , Fósforo/análise , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , Purificação da Água/métodos
3.
Energy Build ; 279: 112681, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36438077

RESUMO

With the outbreak of infectious diseases such as Corona Virus Disease 2019, medical staff work intensively in isolated plots, medical disposable protective clothing (MDPC) has poor air condition and humidity permeability, which seriously reduces the thermal comfort of medical staff. In this paper, the effect of indoor thermal environment and activity levels on thermal comfort inside MDPC was studied by experiment. Five parts of the body were measured inside MDPC and the appropriate movements were chosen to simulate different levels of labor intensity. Meanwhile, physiological parameters and subjective thermal sensation were statistically analyzed. The results showed the influence range of different indoor temperatures on the temperature and humidity inside MDPC was about 1 °C and 10 %, respectively; it indicated that the environment inside MDPC could be improved by reducing indoor temperature, that is, a cross intelligent adjustment mode was proposed. The effect of labor intensity on the temperature inside MDPC was significantly less than that of humidity. Within 20 min, the humidity changes under moderate and heavy labor intensity were even more than 10 %, and the subjective discomfort threshold of the subjects increased by nearly 50 %. Furthermore, the maximum benefit could be obtained by concentrating cooling on back, forehead, chest and upper arm. Theoretical models of working time, labor intensity, and temperature and humidity inside MDPC under different indoor temperatures and different parts were given. In addition, acceptable regions inside MDPC which were approximately parallelogram in the enthalpy-humidity chart. These conclusions could be a reference for future thermal comfort inside MDPC research.

4.
Nature ; 465(7294): 53-9, 2010 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-20445623

RESUMO

Alternative splicing has a crucial role in the generation of biological complexity, and its misregulation is often involved in human disease. Here we describe the assembly of a 'splicing code', which uses combinations of hundreds of RNA features to predict tissue-dependent changes in alternative splicing for thousands of exons. The code determines new classes of splicing patterns, identifies distinct regulatory programs in different tissues, and identifies mutation-verified regulatory sequences. Widespread regulatory strategies are revealed, including the use of unexpectedly large combinations of features, the establishment of low exon inclusion levels that are overcome by features in specific tissues, the appearance of features deeper into introns than previously appreciated, and the modulation of splice variant levels by transcript structure characteristics. The code detected a class of exons whose inclusion silences expression in adult tissues by activating nonsense-mediated messenger RNA decay, but whose exclusion promotes expression during embryogenesis. The code facilitates the discovery and detailed characterization of regulated alternative splicing events on a genome-wide scale.


Assuntos
Processamento Alternativo/genética , Regulação da Expressão Gênica , Código Genético/genética , Modelos Genéticos , RNA Mensageiro/metabolismo , Animais , Inativação Gênica , Humanos , Camundongos , Reprodutibilidade dos Testes
5.
Sci Rep ; 14(1): 15115, 2024 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-38956147

RESUMO

Rapid urbanization increases psychological stress among pedestrians, potentially heightening mental health disorders. This study examines the role of street walls' visual and textural characteristics in stress recovery, using Qingdao as a case study. Virtual reality is employed to simulate five distinct street walls: yellow mortar, brown stone, red brick, green plant, and white mortar. The stress recovery effectiveness of these walls was evaluated through psychological and physiological indicators from 48 young college students. Results indicated that street walls with warm tones, particularly brown stone, significantly aid stress recovery. Psychologically, Restorative Components Scale was highest for brown stone at 1.13. Physiologically, it was linked with notable reductions in diastolic and pulse pressure (decreases of 2.95 mmHg and 2.27 mmHg, respectively), and enhanced parasympathetic activity, as evidenced by the fastest decrease in low frequency/high frequency ratio (LF/HF), and increases in pNN50 and RR (0.14-2.01% and 1.57-11.81 ms, respectively). For urban design, the incorporation of warm-toned materials and natural elements like stone is recommended for their superior restorative benefits.


Assuntos
Estresse Psicológico , Humanos , Masculino , Adulto Jovem , Feminino , Adulto , Urbanização
6.
Sci Rep ; 14(1): 15341, 2024 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-38961227

RESUMO

The physical characteristics of classrooms can significantly impact the physical and mental health as well as the learning performance of college students. This study investigates the effects of classroom size and ceiling height on learning performance using virtual reality technology. Four classroom settings were created: two small classrooms (40.5 m2) with ceiling heights of 3.0 m and 3.9 m, and two large classrooms (62.1 m2) with ceiling heights of 3.9 m and 4.8 m. 34 students participated in task tests while their subjective evaluations and physiological data were recorded. Results showed higher subjective ratings in larger classrooms with the same ceiling height. Classroom size did not significantly affect task test scores. However, there is a significant difference in Task test scores for ceilings of different heights with the same size classroom. The task test improved by 17.3% in the Big and High Room (BHR) and by 20.1% in the Small and Low Room (SLR). Physiological data revealed significant effects of ceiling height, with HRV-nLF/nHF and EEG-ß power increasing by 26.5% and 53.9% in BHR, and by 10.7% and 22.8% in SLR, respectively. This study concludes that classroom size and ceiling height plays a crucial role in learning performance and provides insights for classroom design. It also establishes a framework for future research on the interplay between heart rate variability, EEG, and learning performance.


Assuntos
Aprendizagem , Estudantes , Realidade Virtual , Humanos , Masculino , Feminino , Adulto Jovem , Aprendizagem/fisiologia , Universidades , Adulto
7.
Sci Total Environ ; 951: 175668, 2024 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-39179044

RESUMO

Employing recent short-term historical rainfall data may enhance the performance of rainwater harvesting systems (RWHs) in response to climate change. However, this assumption lacks extensive research, and the evaluation of RWHs currently relies on long-term historical rainfall time series. This study evaluates the feasibility of this assumption and aims to identify the optimal rainfall time series for evaluating RWH performance under climate change. We evaluated RWHs in residential buildings across 16 Japanese cities utilizing historical rainfall time series of varying lengths and 30-year predicted rainfall time series. The minimum rainfall time series length was obtained based on the similarity index between the evaluation results for historical and future periods. The corresponding optimal series can be determined from the distribution of similarity indices in the minimum length. Finally, we introduce supply pressure indices (SPIs) to summarize the rainfall characteristics of these optimal rainfall time series. Our findings highlight that the minimum rainfall time series length increased from 1 year to 30 years as building non-potable water demand rose and city locations varied. Utilizing rainfall time series incorporating recent rainfall data yielded more dependable evaluation results for RWHs under climate change. These optimal rainfall time series share common characteristics with SPIs ranging from 5.37 to 17.87 mm/d, contingent on the local rainfall patterns. Our study concludes that utilizing recent short-term historical rainfall data is feasible to evaluate and design RWHs under climate change.

8.
Genes (Basel) ; 15(2)2024 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-38397131

RESUMO

PURPOSE: The purpose of this study was to screen the genes and pathways that are involved in spermatogonia stem cell (SSC) differentiation regulation during the transition from Aundiff to A1. Methods: RNA sequencing was performed to screen differentially expressed genes at 1 d and 2 d after SSC differentiation culture. KEGG pathway enrichment and GO function analysis were performed to reveal the genes and pathways related to the initiation of early SSC differentiation. RESULTS: The GO analysis showed that Rpl21, which regulates cell differentiation initiation, significantly increased after 1 day of SSC differentiation. The expressions of Fn1, Cd9, Fgf2, Itgb1, Epha2, Ctgf, Cttn, Timp2 and Fgfr1, which are related to promoting differentiation, were up-regulated after 2 days of SSC differentiation. The analysis of the KEGG pathway revealed that RNA transport is the most enriched pathway 1 day after SSC differentiation. Hspa2, which promotes the differentiation of male reproductive cells, and Cdkn2a, which participates in the cell cycle, were significantly up-regulated. The p53 pathway and MAPK pathway were the most enriched pathways 2 days after SSC differentiation. Cdkn1a, Hmga2, Thbs1 and Cdkn2a, microRNAs that promote cell differentiation, were also significantly up-regulated. CONCLUSIONS: RNA transport, the MAPK pathway and the p53 pathway may play vital roles in early SSC differentiation, and Rpl21, Fn1, Cd9, Fgf2, Itgb1, Epha2, Ctgf, Cttn, Timp2, Fgfr1, Hspa2, Cdkn2a, Cdkn1a, Hmga2 and Thbs1 are involved in the initiation of SSC differentiation. The findings of this study provide a reference for further revelations of the regulatory mechanism of SSC differentiation.


Assuntos
Fator 2 de Crescimento de Fibroblastos , Proteína Supressora de Tumor p53 , Masculino , Humanos , Fator 2 de Crescimento de Fibroblastos/metabolismo , Proteína Supressora de Tumor p53/genética , Espermatogônias/metabolismo , Diferenciação Celular/genética , Perfilação da Expressão Gênica
9.
Dalton Trans ; 53(5): 2048-2054, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-38179865

RESUMO

The construction of highly active and stable transition phosphide-based materials is widely regarded as an alternative approach to the use of Pt-based catalysts in the field of electrocatalytic hydrogen evolution. Herein, self-supported heterostructure Co-Ni phosphides (denoted as CoxNi1-x-P) were synthesized with different metal ratios by a low temperature electrodeposition strategy. Impressively, the optimized heterogeneous Co0.5Ni0.5-P nanocomposites displayed outstanding hydrogen evolution performance, with low overpotentials of 67 mV and 181 mV to deliver current densities of 10 mA cm-2 and 100 mA cm-2 in alkaline electrolyte. X-ray photoelectron spectroscopy revealed the optimized electronic structure of Co0.5Ni0.5-P, which led to an improvement in the conductivity. Density functional theory calculations demonstrated that the Co0.5Ni0.5-P heterostructure could provide a more optimal water-dissociation-related Volmer process for hydrogen evolution reaction (HER), in which water molecules could be easily activated on Co0.5Ni0.5-P with a low energy barrier. Moreover, the downshift of the d-band center confirmed the optimized H adsorption, further accelerating the HER kinetics.

10.
Sci Rep ; 14(1): 6279, 2024 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-38491082

RESUMO

Along with the further integration of demand management and renewable energy technology, making optimal use of energy storage devices and coordinating operation with other devices are key. The present study takes into account the current situation of power storage equipment. Based on one year of measured data, four cases are designed for a composite energy storage system (ESS). In this paper, a two-tiered optimization model is proposed and is used to optimizing the capacity of power storage devices and the yearly production of the system. Furthermore, this paper performs a comparative analysis of the performance of the four cases from the energy, environmental and economic perspectives. It is concluded that this kind of energy storage equipment can enhance the economics and environment of residential energy systems. The thermal energy storage system (TESS) has the shortest payback period (7.84 years), and the CO2 emissions are the lowest. Coupled with future price volatility and the carbon tax, the electrothermal hybrid energy storage system (HESS) has good development potential. However, the current investment cost is very high, and it will not be possible to recover this cost in 10 years. Finally, it is recommended that the cost of equipment be reduced in combination with subsidies and incentives for further promotion. The research results not only fill a gap in the study area, but also provide some suggestions for further development of industry and research on user-side energy storage.

11.
Sci Rep ; 14(1): 24252, 2024 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-39414841

RESUMO

Sponge city stormwater management (SCSM) strategy in China aims for sustainable stormwater handling. While many studies have examined the technical aspects of sponge city green infrastructure (SCGI), few have explored public perspectives. This study sought to understand public perceptions, the perceived value of SCGI's ecosystem service benefits, and the potential for diverse financial compensation methods in sponge city construction. A survey conducted in five Northeastern Chinese cities, involving 1,534 participants, was analyzed using descriptive statistics and logistic regression. The findings reveal no significant correlation between socio-demographic factors and understanding of stormwater management, indicating the concept's broad accessibility. Public valuation of ecosystem services showed clear priorities, and factors like homeownership and flood experiences significantly impacted the valuation of specific services. Moreover, the study identified a generally positive public attitude towards investing in SCSM, particularly through stormwater fees, underscoring the viability of diverse funding mechanisms. These insights are pivotal for policymakers and urban planners in formulating sustainable and resilient urban water management strategies.

12.
Sci Rep ; 14(1): 25350, 2024 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-39455663

RESUMO

Climate change and its negative effects are driving the global shift from fossil fuels to renewable energy sources. To tackle the dependency on traditional energy sources in harsh winter regions and improve heating quality during periods of thermal demand fluctuations, this paper proposes a new distributed heating peak shaving system (DHPS). The system combines municipal heat and clean energy within the secondary network while reducing the return water temperature in the primary network. It comprises solar collectors, electric thermal storage tanks (ETST), and absorption heat pump (AHP) units, integrated into conventional heat exchange stations. The system operates in two modes to manage peak and off-peak loads respectively, with TRNSYS simulation used to evaluate performance across a range of peak-shaving gradients. A multidimensional comprehensive assessment is conducted between the DHPS under optimal peak shaving coefficient (θ) conditions and conventional peak clipping boiler (PCB). Results indicate that DHPS achieves a high primary energy ratio (PER) of 1.251 at θ = 0.5, reducing combustion emissions by nearly 40%. The static payback period (PBP) of the system is 3.5 years. When the electricity price drops to 0.275 CNY, its operational costs are comparable to PCB. DHPS caters to the energy characteristics of cold regions where electricity supply exceeds demand. It enables flexible peak shaving while ensuring the complete utilization of clean energy and effectively utilizing waste heat from power plants.

13.
Zhonghua Nan Ke Xue ; 19(4): 315-20, 2013 Apr.
Artigo em Zh | MEDLINE | ID: mdl-23678709

RESUMO

OBJECTIVE: To explore the expressions of SIgA and alpha l-AR in benign prostatic hyperplasia (BPH) complicated by chronic prostatitis (CP) and their implications. METHODS: According to the preoperative findings of expressed prostatic secretion (EPS), transrectal prostate ultrasonography, prostate-specific antigen (PSA), international prostate symptom score (IPSS), clinical symptoms, chronic pelvic pain syndrome (CPPS) and postoperative histopathology, 62 cases of BPH pathologically confirmed after transurethral plasma kinetic resection of the prostate (PKRP) were divided into a BPH group (n = 32) and a BPH + CP group (n = 30). The expressions of SIgA and alpha 1-AR in the prostate tissue were determined by immunohistochemistry and PT-PCR. RESULTS: Of the 62 cases, 30 were found to be BPH + CP, and the other 32 to be BPH. The expressions of SIgA and alpha1-AR were significantly higher in the BPH + CP than in the BPH group (0.380 8 +/- 0.144 3 vs 0.295 4 +/- 0.008 4 and 0.440 5 +/- 0.104 1 vs 0.383 2 +/- 0.013 6, P < 0.05). CONCLUSION: The upregulated expressions of SIgA and alpha1-AR expression in BPH complicated by CP suggest a certain association between CP and BPH, and that inflammation may be a pathogenic factor of BPH and correlate with its pathological development.


Assuntos
Imunoglobulina A Secretora/metabolismo , Próstata/metabolismo , Hiperplasia Prostática/metabolismo , Prostatite/metabolismo , Receptores Androgênicos/metabolismo , Idoso , Doença Crônica , Humanos , Masculino , Pessoa de Meia-Idade , Próstata/patologia , Hiperplasia Prostática/complicações , Hiperplasia Prostática/patologia , Prostatite/complicações , Prostatite/patologia
14.
Bioresour Technol ; 388: 129775, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37722539

RESUMO

Microorganisms play key roles in the conversion of organic matter in foodwaste. However, both the microbially-mediated element (carbon/C and nitrogen/N) flows and associated microbial communities in foodwaste treatment plants (FWTPs) remain unclear. This study collected samples of different foodwaste treatment units from five full-scale FWTPs to analyze the C/N flows and microbial communities in foodwaste treatment processes. Results showed that 39.8-45.0% of organic carbon in foodwaste was converted into biogas. Hydrolytic acidogenic bacteria (e.g., Lactobacillus and Limosilactobacillus) and eukaryota (e.g., Cafeteriaceae, Saccharomycetales, and Agaricomycetes) were more abundant in feedstock and pretreatment units. Redundancy analyses showed that acidogens were major players in the transformation of foodwaste organic matter. Populations of W27 and Tepidanaerobacter were major contributors to the difference in conversion of C/N in these FWTPs. This study could support foodwaste treatment efficiencies improvement by providing insights into C/N flows and associated microbiota in FWTPs.

15.
Heliyon ; 9(9): e19823, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37809907

RESUMO

Accurate and detailed solar radiation data play a crucial role in the simulation of building thermal and photovoltaic systems. However, developing a highly precise and dependable solar radiation model using cost-effective data has proven challenging. This work proposes a new attenuation solar radiation model formed by conducting a comprehensive analysis of existing models and gaining new insights into solar radiation's seasonal and stochastic properties. Meanwhile, the model is constructed using easily obtainable surface meteorological parameters. The results demonstrate that the proposed model exhibits good performance in terms of prediction accuracy. Moreover, the majority of existing hourly solar radiation models have been primarily developed for clear-sky conditions. However, there is a growing demand for solar radiation hourly estimations that can uphold a high level of accuracy and reliability even in different weather state. Conversely, the proposed model is developed and validated by more than twenty year's meteorological data encompassing various weather conditions in Japan. It effectively captures the stochastic nature of solar radiation by utilizing turbidity parameters, even on cloudy and rainy days. Additionally, the inclusion of interaction variables significantly enhances its interpretability.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36767883

RESUMO

The development of ecological restoration projects is unsatisfactory, and soil erosion is still a problem in ecologically restored areas. Traditional soil erosion studies are mostly based on satellite remote sensing data and traditional soil erosion models, which cannot accurately characterize the soil erosion conditions in ecological restoration areas (mainly plantation forests). This paper uses high-resolution unmanned aerial vehicle (UAV) images as the base data, which could improve the accuracy of the study. Considering that traditional soil erosion models cannot accurately express the complex relationships between erosion factors, this paper applies convolutional neural network (CNN) models to identify the soil erosion intensity in ecological restoration areas, which can solve the problem of nonlinear mapping of soil erosion. In this study area, compared with the traditional method, the accuracy of soil erosion identification by applying the CNN model improved by 25.57%, which is better than baseline methods. In addition, based on research results, this paper analyses the relationship between land use type, vegetation cover, and slope and soil erosion. This study makes five recommendations for the prevention and control of soil erosion in the ecological restoration area, which provides a scientific basis and decision reference for subsequent ecological restoration decisions.


Assuntos
Erosão do Solo , Solo , Florestas , Redes Neurais de Computação , Conservação dos Recursos Naturais/métodos
17.
ACS Omega ; 8(30): 27794-27801, 2023 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-37546604

RESUMO

Coal fly ash (CFA) is one of the industrial byproducts of burning coal for energy production and has unburned carbon, which negatively affects its full potential use. The flotation technology can be effective in separating unburned carbon from CFA, and the prestirring time is crucial for the ideal initial conditions during the flotation process. To find the suitable prestirring time, eight prestirring times, including 0, 1, 2, 3, 4, 5, 6, and 7 min, were selected in this paper, followed by flotation of CFA after prestirring. Parameters such as loss on ignition (LOI), the removal rate of unburned carbon (RUC), contact angle, and particle size volume fraction were used to assess the effect of prestirring time on flotation results. The results showed that the prestirring time significantly affects the CFA flotation performance. As the prestirring time increased, the LOI of CFA first decreased and then increased, and the contact angle showed the opposite trend. Besides, the prestirring time of over 2 min positively affected the fineness of the tailings. Overall, the prestirring time of 3 min had the most significant carbon removal effect, obtaining an LOI of tailings of 0.96%, a yield of 74.56%, an RUC of 72.70%, and a volume fraction less than 45 µm of 36.65%. This study provides theoretical support for improving stirring efficiency and saving flotation costs in industrial applications and is conducive to the recycling of CFA resources.

18.
Dalton Trans ; 52(26): 8998-9004, 2023 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-37334505

RESUMO

The hydrogen evolution reaction (HER) plays a vital role for the production of pure hydrogen with zero carbon release. Developing high efficiency non-noble metal electrocatalysts could reduce its cost. Here, vanadium doped cobalt phosphide grown on carbon cloth (CC) was synthesized by the low temperature electrodeposition-phosphorization method. The influence of V dopants on the structural, morphological, and electrocatalytic performance of Vx-Co1-x-P composites was also investigated in-depth. Impressively, the optimized amorphous V0.1-Co0.9-P nano-electrocatalyst exhibits outstanding catalytic activity with a low overpotential of 50 mV at a current density of 10 mA cm-2 and a small Tafel value of 48.5 mV dec-1 in alkaline media. The results showed that V dopants in the composite change its crystal structure from the crystalline phase to the amorphous phase, resulting in the introduction of V-O sites, which regulate the electron density of the active sites and the exposure of surface active sites and thus promote the electrocatalytic HER process. This work provides a novel idea for the fabrication of high-efficiency metal phosphide based electrocatalysts.

19.
Heliyon ; 9(10): e20646, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37860505

RESUMO

Nowadays, evaporatively cooled office buildings commonly observed in dry hot areas in summer of China. However, few dedicated studies to record the local residents' thermal comfort and adaptability in these buildings. The contribution of adaptive comfort theory on thermal perception still remains unclear for optimizing office building design parameters. Hence, to deeper probe the adaptive thermal comfort of the related indoor environment, a field study of office buildings during summer considering evaporative cooling air conditioned (ECA) and naturally ventilated (NV) mode was conducted in Turpan, China. Based on 931 valid datasets collected from questionnaires, we found that the neutral temperature (Tn) of 28.4 °C in ECA group, 0.6 °C lower than NV group (29.0 °C). A lower air temperature (Ta) and higher humidity (RH)/air-velocity (Va) were expected in two modes, and Va has a stronger influence than RH on mean thermal sensation votes (MTSV). Meanwhile, occupants can adapt to current indoor environment through physiological, psychological and behavioral adjustments, while the clothing regulation had limited effect on MTSV unless the outdoor temperature exceeds 38 °C. Whether in ECA or NV mode, the predicted mean votes (PMV) model overestimated actual thermal sensation when operative temperature (Top) beyond 28 °C. Adaptive models were also proved varied from that in current standards, which indicated that they were not suitable for evaluating the studied buildings in Turpan. Above findings could suggest us a better understanding of the occupants' thermal adaptability, thereby providing the reference of design parameters revision and passive strategies for local newly/renovated buildings.

20.
Reprod Sci ; 30(6): 1938-1951, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-36538028

RESUMO

As one of the factors of male infertility, high temperature induces apoptosis of differentiated spermatogenic cells, sperm DNA oxidative damage, and changes in morphology and function of Sertoli cells. Spermatogonial stem cells (SSCs) are a type of germline stem cells that maintain spermatogenesis through self-renewal and differentiation. At present, however, the effect of high temperature on SSC differentiation remains unknown. In this study, an in vitro SSC differentiation model was used to investigate the effect of heat stress treatment on SSC differentiation, and RNA sequencing (RNA-seq) was used to enrich the key genes and pathways in high temperature inhibiting SSC differentiation. Results show that 2 days of 37 °C or 43 °C (30 min per day) heat stress treatment significantly inhibited SSC differentiation. The differentiation-related genes c-kit, stra8, Rec8, Sycp3, and Ovol1 were down-regulated after 2 and 4 days of heat stress at 37 °C. The transcriptome of SSCs was significantly differentially expressed on days 2 and 4 after heat stress treatment at 37 °C. In total, 1660 and 7252 differentially expressed genes (DEGs) were identified by RNA-seq in SSCs treated with heat stress at 37 °C for 2 and 4 days, respectively. KEGG pathway analysis showed that p53, ribosome, and carbon metabolism signaling pathways promoting stem cell differentiation were significantly enriched after heat stress treatment at 37 °C. In conclusion, 37 °C significantly inhibited SSC differentiation, and p53, ribosome, and carbon metabolism signaling pathways were involved in this differentiation inhibition process. The results of this study provide a reference for further investigation into the mechanism by which high temperature inhibits SSC differentiation.


Assuntos
Espermatogônias , Proteína Supressora de Tumor p53 , Masculino , Humanos , Espermatogônias/metabolismo , Temperatura , Proteína Supressora de Tumor p53/metabolismo , Sêmen , Diferenciação Celular , Espermatogênese/fisiologia , Perfilação da Expressão Gênica
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