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1.
Ren Fail ; 39(1): 236-245, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27846768

RESUMO

Acrylamide (ACR) is one of the most important contaminants occurring in foods heated at high temperatures. The aim of this study is to investigate the protective efficacy of extra virgin olive oil (EVOO), a main component of the Mediterranean diet, against nephrotoxicity induced by ACR. Rats have received by gavage during 21 days either ACR (40 mg/kg body weight) or ACR-associated with EVOO (300 µl) or only EVOO (300 µl). Acrylamide induced nephrotoxicity as evidenced by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), protein carbonyls (PCOs) and a decrease in glutathione, non-protein thiols (NPSHs), and vitamin C levels. Activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) were also decreased. Lactate dehydrogenase (LDH) activity, creatinine, urea, and uric acid, urinary volume and creatinine clearance levels were modified. EVOO supplementation improved all the parameters indicated above. Kidney histoarchitecture confirmed the biochemical parameters and the beneficial role of EVOO. EVOO, when added to the diet, may have a beneficial role against kidney injury by scavenging free radicals and by its potent antioxidant power.


Assuntos
Acrilamida/toxicidade , Antioxidantes/farmacologia , Nefropatias/prevenção & controle , Azeite de Oliva/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Animais , Catalase/metabolismo , Suplementos Nutricionais , Feminino , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Peróxido de Hidrogênio/metabolismo , Rim/fisiopatologia , L-Lactato Desidrogenase/sangue , Malondialdeído/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo
2.
Biochem Genet ; 52(5-6): 258-68, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24535154

RESUMO

We used eight informative microsatellite markers for fingerprinting and evaluation of genetic similarity among 15 Tunisian olive (Olea europaea L.) cultivars and two feral unknown trees named Soulela 1 and Soulela 2. Thirty-one alleles were revealed, and the number of alleles per SSR varied from 2 (UDO12) to 6 (GAPU71A). Cluster analysis grouped cultivars into three main clusters. The two unknown varieties could not be reliably classified into any of these cultivar groups. SSR analysis indicated the presence of three erroneous denominations of cultivars. We resolved two synonymy cases (Zalmati and Chemlali; Rkhami and Chetoui) and one case of homonymy (Chemlali Tataouine). Genetic analyses of DNA extracted from leaves, oils, and embryos of the two unknown cultivars and the two major Tunisian olive cultivars (Chemlali and Chetoui) were also studied. We conclude that the reliable identification of these two feral cultivars needs to be addressed by a larger set of markers.


Assuntos
Olea/genética , Alelos , Impressões Digitais de DNA , Variação Genética , Repetições de Microssatélites , Azeite de Oliva , Folhas de Planta/genética , Óleos de Plantas , Polimorfismo Genético , Sementes/genética , Tunísia
3.
Food Funct ; 6(9): 3098-108, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26215160

RESUMO

Oxidative stress generated by an excessive production of free radicals has been linked to the development of several health problems such as cardiovascular diseases. We investigated the protective efficacy of Extra Virgin Olive Oil (EVOO) and its lipophilic fraction (OOLF) and hydrophilic fraction (OOHF) against the cardiotoxicity and DNA damage induced by co-exposure to aluminum (AlCl3) and acrylamide (ACR). Rats were divided into eight groups of six each: controls, AlCl3 (50 mg per kg body weight) administered via drinking water and ACR (20 mg per kg body weight) given by gavage, combined group plus EVOO (300 µl); combined group plus the hydrophilic fraction (1 ml); combined group plus the lipophilic fraction (300 µl); extra virgin olive oil (EVOO) and its fractions were administered daily by gavage for 21 days. Three other groups, considered as positive controls, received either EVOO, OOLF or OOLH. Exposure of rats to both AlCl3 and ACR provoked oxidative stress objectified by an increase in MDA, AOPP and a decrease in GSH, NPSH and vitamin C levels. The activities of CAT, GPx and SOD were also decreased. EVOO and its OOLF fraction exhibited a pronounced enhancement of antioxidant status while a partial recovery in the antioxidant status was obtained with the OOHF fraction. Plasma LDH and CK activities, TC, LDL-C levels, TC/HDL-C and LDL-C/HDL-C ratios were increased, while HDL-C and TG decreased in rats treated with both AlCl3 and ACR. Co-administration of EVOO, OOLF or OOHF to treated rats restored cardiac biomarkers and lipid profile to near-normal values. Histological studies and DNA damage confirmed the biochemical parameters and the beneficial role of EVOO and its two fractions. Our results suggest that extra virgin olive oil and its two fractions can decrease the frequency of cardiac complications and genotoxicity.


Assuntos
Acrilamida/toxicidade , Alumínio/toxicidade , Doenças Cardiovasculares/dietoterapia , Dano ao DNA/efeitos dos fármacos , Miocárdio/metabolismo , Azeite de Oliva/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Animais , Doenças Cardiovasculares/induzido quimicamente , Doenças Cardiovasculares/genética , Doenças Cardiovasculares/metabolismo , Coração/efeitos dos fármacos , Humanos , Masculino , Olea/metabolismo , Azeite de Oliva/química , Ratos
5.
PLoS One ; 8(5): e62831, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23690957

RESUMO

One of the challenge fruit growers are facing is to balance between tree production and vegetative growth from year to year. To investigate the existence of genetic determinism for reproductive behaviour in olive tree, we studied an olive segregating population derived from a cross between 'Olivière' and 'Arbequina' cultivars. Our strategy was based on (i) an annual assessment of individual trees yield, and (ii) a decomposition of adult growth units at the crown periphery into quantitative variables related to both flowering and fruiting process in relation to their growth and branching. Genetic models, including the year, genotype effects and their interactions, were built with variance function and correlation structure of residuals when necessary. Among the progeny, trees were either 'ON' or 'OFF' for a given year and patterns of regular vs. irregular bearing were revealed. Genotype effect was significant on yield but not for flowering traits at growth unit (GU) scale, whereas the interaction between genotype and year was significant for both traits. A strong genetic effect was found for all fruiting traits without interaction with the year. Based on the new constructed genetic map, QTLs with small effects were detected, revealing multigenic control of the studied traits. Many were associated to alleles from 'Arbequina'. Genetic correlations were found between Yield and Fruit set at GU scale suggesting a common genetic control, even though QTL co-localisations were in spe`cific years only. Most QTL were associated to flowering traits in specific years, even though reproductive traits at GU scale did not capture the bearing status of the trees in a given year. Results were also interpreted with respect to ontogenetic changes of growth and branching, and an alternative sampling strategy was proposed for capturing tree fruiting behaviour. Regular bearing progenies were identified and could constitute innovative material for selection programs.


Assuntos
Flores/genética , Frutas/genética , Olea/genética , Locos de Características Quantitativas/genética , Mapeamento Cromossômico , Cruzamentos Genéticos , Genótipo , Modelos Genéticos , Olea/crescimento & desenvolvimento
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