RESUMO
This report describes 2 events of degenerative myelopathy in 4- to 27-day-old piglets, with mortality rates reaching 40%. Sows were fed rations containing low levels of pantothenic acid. Piglets presented with severe depression, weakness, ataxia, and paresis, which were more pronounced in the pelvic limbs. No significant gross lesions were observed. Histologically, there were degeneration and necrosis of neurons in the spinal cord, primarily in the thoracic nucleus in the thoracic and lumbar segments, and motor neurons in nucleus IX of the ventral horn in the cervical and lumbar intumescence. Minimal-to-moderate axonal and myelin degeneration was observed in the dorsal funiculus of the spinal cord and in the dorsal and ventral nerve roots. Immunohistochemistry demonstrated depletion of acetylcholine neurotransmitters in motor neurons and accumulation of neurofilaments in the perikaryon of neurons in the thoracic nucleus and motor neurons. Ultrastructurally, the thoracic nucleus neurons and motor neurons showed dissolution of Nissl granulation. The topographical distribution of the lesions indicates damage to the second-order neurons of the spinocerebellar tract, first-order axon cuneocerebellar tract, and dorsal column-medial lemniscus pathway as the cause of the conscious and unconscious proprioceptive deficit, and damage to the alpha motor neuron as the cause of the motor deficit. Clinical signs reversed and no new cases occurred after pantothenic acid levels were corrected in the ration, and piglets received parenteral administration of pantothenic acid. This study highlights the important and practical use of detailed neuropathological analysis to refine differential diagnosis.
Assuntos
Doenças da Medula Espinal , Doenças dos Suínos , Animais , Suínos , Feminino , Ácido Pantotênico/metabolismo , Medula Espinal/patologia , Neurônios/patologia , Bulbo/patologia , Doenças da Medula Espinal/veterinária , Doenças da Medula Espinal/metabolismo , Doenças da Medula Espinal/patologia , Doenças dos Suínos/patologiaRESUMO
Ocular involvement in systemic diseases is frequent in cats; however, without concurrent clinical and ophthalmic examinations with gross and/or histologic analysis of the eye, these findings can be underdiagnosed. This article aims to provide gross, histologic, and immunohistochemical characteristics of ocular lesions from cats submitted to necropsy, focusing on those caused by systemic infectious agents. Cats that died due to a systemic infectious disease were selected based on necropsy diagnosis and presence of ocular lesions. Gross, histologic, and immunohistochemical findings were recorded. From April 2018 to September 2019, 849 eyes of 428 cats were evaluated. Histologic abnormalities were seen in 29% of cases, which were classified as inflammatory (41%), neoplastic (32%), degenerative (19%), and metabolic/vascular (8%). Macroscopic changes were present in one-third of eyes with histologic lesions. Of these, 40% were attributed to inflammatory or neoplastic diseases associated with infectious agents. The most important infectious agents causing ocular disease in this study were feline leukemia virus, feline infectious peritonitis virus, and Cryptococcus sp. The most common ocular abnormalities associated with infectious agents were uveitis (anterior, posterior, or panuveitis), optic neuritis, and meningitis of the optic nerve. Ocular lesions secondary to systemic infections in cats are frequent; however, these are not always diagnosed because gross lesions are less common than histologic lesions. Therefore, both gross and histologic evaluation of the eyes of cats is recommended, mainly for cases in which the clinical suspicion or necropsy diagnosis suggests that an infectious agent might be related to the cause of death.
Assuntos
Doenças do Gato , Doenças Transmissíveis , Peritonite Infecciosa Felina , Neoplasias , Sepse , Uveíte , Gatos , Animais , Olho/patologia , Uveíte/patologia , Uveíte/veterinária , Neoplasias/patologia , Neoplasias/veterinária , Sepse/patologia , Sepse/veterinária , Doenças Transmissíveis/patologia , Doenças Transmissíveis/veterinária , Doenças do Gato/patologia , Peritonite Infecciosa Felina/patologiaRESUMO
The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined. The nonstructural protein (NS1) amino acid (aa) sequence of this virus shared less than 30% identity with those of other copiparvoviruses and less than 22% identity with those of members of other genera in the subfamily Parvovirinae. Phylogenetically, this virus, which we have provisionally named "sheep copiparvovirus 1", formed a cluster with copiparvovirus sequences and should be classified as a member of a new species in the genus Copiparvovirus.
Assuntos
Infecções por Parvoviridae/veterinária , Parvovirinae/genética , Doenças dos Ovinos/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Brasil/epidemiologia , DNA Viral/genética , Feminino , Genoma Viral/genética , Masculino , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Parvovirinae/classificação , Filogenia , Ovinos , Doenças dos Ovinos/epidemiologia , Proteínas Virais/genéticaRESUMO
The aim of this study was to evaluate in vitro the efficacy of cordycepin and pentostatin (alone or combined) against Trypanosoma cruzi, as well as the therapeutic efficiency of protocols of cordycepin and pentostatin combinations in mice experimentally infected with T. cruzi. In vitro, the cordycepin (3'-deoxyadenosine) and pentostatin (deoxycoformycin) exerted potent trypanocidal effect against T. cruzi (Colombian strain), similarly to benznidazole, which is the reference drug. For epimastigotes, the lethal dose of cordycepin capable of killing 50% (LD50) and 20% (LD20) of the parasites was 0.072 and 0.031â¯mg/mL, respectively and for trypomastigotes was 0.047 and 0.015â¯mg/mL, respectively. The combined use of cordycepin and pentostatin resulted in a LD50 and LD20 for epimastigotes of 0.068 and 0.027â¯mg/mL, respectively, as well as 0.056 and 0.018â¯mg/mL for trypomastigotes, respectively. In vivo, the combined use of cordycepin and pentostatin did not show the expected curative effect, however it was able to control the parasitema in the peak period. In summary, the combination of cordycepin and pentostatin showed no curative effect in mice infected by T. cruzi, despite the in vitro reduction of epimastigotes and trypomastigotes.
Assuntos
Antiprotozoários/farmacologia , Doença de Chagas/tratamento farmacológico , Desoxiadenosinas/farmacologia , Pentostatina/farmacologia , Trypanosoma cruzi/efeitos dos fármacos , Análise de Variância , Animais , Antiprotozoários/efeitos adversos , Antiprotozoários/uso terapêutico , Doença de Chagas/parasitologia , Desoxiadenosinas/uso terapêutico , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Feminino , Coração/efeitos dos fármacos , Dose Letal Mediana , Camundongos , Miocárdio/patologia , Doenças Negligenciadas/tratamento farmacológico , Doenças Negligenciadas/parasitologia , Nifurtimox/efeitos adversos , Nifurtimox/uso terapêutico , Nitroimidazóis/efeitos adversos , Nitroimidazóis/uso terapêutico , Dinâmica não Linear , Parasitemia/prevenção & controle , Pentostatina/uso terapêutico , Distribuição Aleatória , Análise de RegressãoRESUMO
Southern right whales Eubalaena australis (SRW) use the southern coast of Brazil as a wintering and calving ground. Other than anthropogenic threats, there is limited knowledge on health and disease aspects for this species. We report the gross and microscopic findings and microbiological identification of streptococcal septicemia in a SRW calf. Main gross findings included fibrinosuppurative omphalitis and urachocystitis, suppurative cystitis, valvular endocarditis and myocarditis, embolic pneumonia, suppurative myositis and osteoarthritis, and lymphadenomegaly. Histological examination confirmed the above inflammatory processes and indicated disseminated Gram-positive coccoid septicemia. PCR analysis, based on the 16S rRNA gene from bacteria isolated on blood agar, identified Streptococcus dysgalactiae. Pathologic and microbiologic analysis indicated that ß-hemolytic S. dysgalactiae septicemia, presumably initiated as ascending omphalic infection, was responsible for stranding and death in this individual. These results further confirm pathogenicity of streptococci in cetaceans and add to the limited health and disease related pathology knowledge for this species.
Assuntos
Sepse , Infecções Estreptocócicas , Animais , Brasil , RNA Ribossômico 16S , Sepse/veterinária , Infecções Estreptocócicas/veterinária , Streptococcus , BaleiasRESUMO
The aim of this study was to evaluate the efficacy of 3'-deoxyadenosine and deoxycoformycin combination in the treatment of mice infected by T. cruzi, as well as to verify the influence of the treatment on purinergic enzymes. Heart and serum samples were collected from 60 mice (30 infected and 30 uninfected) at day 12 post-infection. To verify treatment efficacy, parasitemia was monitored, and the treatment with 3'-deoxy adenosine and deoxycoformycin combination was able to reduce it, but had no curative effect on mice. Seric activities of NTPDase (ATP and ADP substrate) and ADA were increased significantly in untreated mice infected by T. cruzi compared to the negative control, as well as mice treated with 3'-deoxyadenosine and deoxycoformycin (alone or combined) modulated the activity of NTPDase (ATP and ADP substrate), preventing them from increasing in infected animals (activity similar to healthy animals). Treatment with deoxycoformycin alone and associated with 3'-deoxyadenosine modulated the activity of ADA preventing them from increasing in infected animals. However, seric activities of ADA in mice treated with 3'-deoxyadenosine (cordycepin) alone does not modify the ADA activity compared with infected and non-treated mice. However, the 5'-nucleotidase activity decreased significantly in infected untreated animals and the same occurred in infected and treated animals with deoxycoformycin and 3'-deoxyadenosine. However, treatment with deoxycoformycin associated with 3'-deoxyadenosine preventing them from decreasing the 5'-nucleotidase activity. Therefore, we conclude that the treatments did not have curative success for mice infected by T. cruzi. However, the treatments were able to modulate the purinergic enzymes during the infection by T. cruzi, which may contribute to reduce the inflammatory damage in heart.
Assuntos
Antiprotozoários/uso terapêutico , Doença de Chagas/tratamento farmacológico , Desoxiadenosinas/uso terapêutico , Parasitemia/tratamento farmacológico , Pentostatina/uso terapêutico , Trypanosoma cruzi/efeitos dos fármacos , Adenosina Desaminase/metabolismo , Animais , Doença de Chagas/parasitologia , Quimioterapia Combinada , Feminino , Camundongos , Parasitemia/parasitologia , Pirofosfatases/metabolismoRESUMO
The enzymatic activities of NTPDase, 5'-nucleotidase and adenosine deaminase (ADA) are important in regulating the concentration of adenine nucleotides, molecules known to be involved on platelet aggregation. Fasciolosis causes coagulation disorders that have not been completely elucidated. Taking into consideration the association between the purinergic system and hemostasis, this study aimed to evaluate the enzymatic activities of NTPDase (hydrolyze ATP and ADP), 5'-nucleotidase (hydrolyze AMP) and ADA (deamination of adenosine) in platelets from cattle experimentally infected by Fasciola hepatica on days 20, 40, 60 and 80 post-infection (PI). For this study, 10 healthy Friesian steers were separated into two groups: the group A (n = 5) was used as uninfected control, and the group B was composed of steers experimentally infected by F. hepatica (n = 5). The number of platelets did not differ between groups in the periods evaluated. Reduction of NTPDase (p < 0.05) hydrolysing ATP (days 20, 40 and 60 PI), and ADP (days 40, 60 and 80 PI), and on 5'-nucleotidase hydrolyzing AMP (days 40 and 60 PI) was observed. A reduction (p < 0.05) in ADA activity on day 20 PI, as well as an increase (p < 0.05) in ADA activity on days 40 and 60 PI was observed when compared to the control. Based on these results, we can conclude that ATP, ADP and AMP hydrolysis and adenosine deamination were altered in platelets of cattle infected by F. hepatica. Considering the importance of the purinergic system in hemostasis, it is believed that those changes may contribute to the coagulation impairment observed in acute fasciolosis described in the literature.
Assuntos
Adenosina Desaminase/sangue , Plaquetas/enzimologia , Doenças dos Bovinos/parasitologia , Fasciolíase/veterinária , Nucleotidases/sangue , Animais , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/enzimologia , Fasciola hepatica/fisiologia , Fasciolíase/sangue , Fasciolíase/enzimologia , Fezes/parasitologia , Fígado/parasitologia , Fígado/patologia , Masculino , Contagem de Ovos de Parasitas/veterinária , Contagem de Plaquetas/veterináriaRESUMO
The aim of this study was to evaluate the activity of purinergic enzymes in lymphocytes and cardiac tissue of mice experimentally infected by Trypanosoma cruzi. Twelve female mice were used, divided into two groups (n = 6): uninfected and infected. On day 12 post-infection (PI), the animals were anesthetized and after euthanized, and samples were collected for analyses. Infected mice showed reduction in erythrocyte counts, hematocrit and hemoglobin concentration, as well as reduced number of total leukocytes in consequence of neutrophilia (P < 0.01). The number of monocytes increased in infected mice (P < 0.001), however the number of lymphocytes and eosinophils did not differ between groups (P > 0.05). The E-NTPDase (ATP and ADP substrate) and E-ADA activities in lymphocytes increased significantly in mice infected by T. cruzi (P < 0.01). In the heart, multiple pseudocysts containing amastigotes within cardiomyocytes were observed, as well as focally extensive severe necrosis associated with diffuse moderate to severe inflammatory infiltrate of lymphocytes. Although, the NTPDase activity (ATP and ADP substrate) in the cardiac homogenate did not differ between groups, a reduction on 5'-nucleotidase activity (P < 0.001) and an increase in the ADA activity in infected animals (P < 0.05) were observed. Thus, animals infected by T. cruzi experienced the disease, i.e., showed anemia, leucopenia, and heart lesions. Associated with this, purinergic enzymes showed altered activities, which might be related to the modulation of the inflammatory response.
Assuntos
Doença de Chagas/enzimologia , Linfócitos/enzimologia , Miócitos Cardíacos/enzimologia , Purinas/metabolismo , 5'-Nucleotidase/metabolismo , Adenosina/metabolismo , Difosfato de Adenosina/metabolismo , Monofosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Antígenos CD/metabolismo , Apirase/metabolismo , Doença de Chagas/patologia , Modelos Animais de Doenças , Feminino , Coração/parasitologia , Testes Hematológicos , Hidrólise , Camundongos , Miocárdio/patologia , Parasitemia/parasitologia , Trypanosoma cruzi/fisiologiaRESUMO
The aim of this study was to evaluate hepatic and seric levels of purines, as well as their breakdown products in rats infected by Fasciola hepatica on days 15 and 87 post-infection (PI). Rats were divided into two groups: uninfected (n = 10) and infected (n = 20). On day 15 (n = 5 for uninfected group and n = 10 for infected group) and 87 PI (n = 5 for uninfected group and n = 10 for infected group), animals were euthanized for sampling to evaluate levels of purines by high-performance liquid chromatography. In serum, ATP increased (P < 0.05) and ADP decreased (P < 0.05) on days 15 and 87 PI, while AMP increased (P < 0.05) only on day 15 PI. Hypoxanthine levels increased (P < 0.05) on days 15 and 87 PI, while adenosine and xanthine levels decreased and increased (P < 0.05), respectively, on day 87 PI. No difference was observed regarding seric inosine and uric acid (P > 0.05). Hepatic ATP, adenosine, and uric acid levels decreased (P < 0.05) on days 15 and 87 PI. AMP levels decreased (P < 0.05) on day 87 PI, while xanthine levels increased (P < 0.05) on day 15 PI in the liver. Also in the liver, hypoxanthine levels increased (P < 0.05) on day 15 PI and decreased (P < 0.05) on day 87 PI. On the other hand, there was no difference on hepatic ADP and inosine levels (P > 0.05). Therefore, it is possible to conclude that F. hepatica infection can change purine levels, which may be associated with an inflammatory process, and these alterations may influence fasciolosis pathogenesis.
Assuntos
Fasciola hepatica/fisiologia , Fasciolíase/parasitologia , Purinas , Nucleotídeos de Adenina/sangue , Nucleotídeos de Adenina/metabolismo , Animais , Fígado/metabolismo , Fígado/patologia , Masculino , Purinas/sangue , Purinas/metabolismo , RatosRESUMO
The aim of this study was to analyze the antioxidant status and oxidative profile in serum and liver of rats experimentally infected with Fasciola hepatica and its relationship with pathological findings. Twenty-four rats were divided into two groups: group A consisted of 12 healthy rats and group B of 12 rats infected orally with 20 metacercaria of F. hepatica. At days 20 and 150 post-infection (PI), blood and liver samples of six animals from each group were collected. The protein oxidation (AOPP technique: advanced oxidation protein products) and antioxidants (FRAP technique: ferric reducing antioxidant power) levels were measured in serum and liver. Furthermore, nitrite/nitrate (NOx) levels and lipid peroxidation (TBARS technique: thiobarbituric acid reactive substances) were measured in liver. AOPP and FRAP levels were increased (P < 0.05) in serum and liver of infected animals in acute and chronic infection when compared with healthy animals. The same occurred with TBARS and NOx levels in the liver (P < 0.05). Histopathology revealed periportal fibrous hepatitis, composed of an abundant inflammatory infiltrate in portal spaces on infected animals, as well as bile duct hyperplasia. The results found seem to be related to the host free radical production demonstrated in serum samples and liver due to the parasite infection.
Assuntos
Fasciolíase/metabolismo , Fígado/metabolismo , Fígado/patologia , Estresse Oxidativo , Produtos da Oxidação Avançada de Proteínas/análise , Animais , Antioxidantes/análise , Antioxidantes/metabolismo , Fasciolíase/complicações , Fasciolíase/patologia , Peroxidação de Lipídeos , Fígado/parasitologia , Cirrose Hepática/etiologia , Cirrose Hepática/metabolismo , Cirrose Hepática/patologia , Masculino , Nitratos/análise , Nitritos/análise , Ratos , Ovinos , Substâncias Reativas com Ácido Tiobarbitúrico/análiseRESUMO
Lymphoma is the most common hematopoietic neoplasm in dogs. However, cases primarily involving the third eyelid are rarely reported in the species. The subtype most frequently described in this location is marginal zone lymphoma of the mucosa-associated lymphoid tissue, which has an indolent behavior. A 10-year-old intact female Poodle was presented with a 2-month history of a nodule in the left third eyelid for evaluation. This nodule was surgically excised; however, local recurrence was detected nineteen days postoperatively, with the formation of a mass that expanded and replaced the extraocular orbital tissues and infiltrated the eye. Euthanasia was elected due to the deteriorating clinical condition, and a necropsy was performed. Macroscopically, the mass infiltrated and expanded the eyelids with narrowing of the palpebral fissure, expanded into the orbital space, and infiltrated the cornea and bulbar conjunctiva. Histologically, the mass was composed of sheets of intermediate to large neoplastic lymphocytes, with frequent epitheliotropism and tingible bodies. Neoplastic lymphocytes were immunoreactive for PAX5, CD20, and CD79a and were not immunoreactive for CD3. The neoplasm was restricted to the left eyelid and orbit. A diagnosis of primary third eyelid lymphoma (extranodal) with immunophenotype B and locally aggressive behavior was made based on the macroscopic, histological, and immunohistochemical findings.
RESUMO
Pasteurella multocida is the main secondary bacterium isolated from cases of swine pneumonia. Although highly pathogenic strains of P. multocida have been associated with primary septic lesions and polyserositis in pigs, studies on this pathological presentation in naturally occurring cases are limited. The aim of this work was to characterize the clinical, pathological and molecular findings in cases of P. multocida polyserositis in growing-finishing pigs in a commercial farm in Brazil. The mean age of 17 investigated pigs was 120 days. Clinically, the disease was acute (11/17), with clinical signs of dyspnoea and apathy. Sudden death occurred in some animals (6/17). The main gross findings included fibrinous serositis affecting the abdominal and thoracic cavities (17/17), fibrinous pericarditis (15/17), marked cranioventral pulmonary consolidation (17/17) and splenic infarcts (3/17). P. multocida was isolated in all cases from systemic sites, including the pericardial sac and abdominal exudate. Molecular typing of genus and species was performed on four isolates, and all were characterized as P. multocida type A. Another five isolates were positive for the pathogenicity marker gene pfhA by polymerase chain reaction. This study reinforces the role of P. multocida as a cause of polyserositis in growing-finishing pigs.
Assuntos
Infecções por Pasteurella , Pasteurella multocida , Doenças dos Suínos , Suínos , Animais , Pasteurella multocida/genética , Infecções por Pasteurella/veterinária , Infecções por Pasteurella/microbiologia , Reação em Cadeia da Polimerase/veterinária , Virulência/genética , BrasilRESUMO
A 7-year-old mixed-breed neutered male cat was presented with a history of chronic vomiting with increased frequency in the last month, progressing to depression and profuse projectile vomiting. Abdominal ultrasonography revealed an area of marked segmental duodenal thickening associated with a nodule, and euthanasia was performed due to a poor prognosis. Grossly, at the postmortem examination, the wall of a duodenal segment was severely expanded by a nodule measuring 5.0 cm in diameter, resulting in marked luminal narrowing and obstruction. Smears were made by scraping the cut surface of the tumor during the postmortem examination. The smears were highly cellular and contained mesenchymal cells arranged individually or in large non-cohesive aggregates. Cells varied in morphology from spindle to round to stellate-shaped with marked anisocytosis and anisokaryosis. Numerous bi- and multinucleated neoplastic cells were observed. Histologic examination revealed a densely cellular neoplastic proliferation of mesenchymal cells that effaced the duodenal submucosa, muscularis externa, and serosa layers. Most cells were spindle-shaped; however, some tumor areas had numerous round cells and bi- and multinucleated neoplastic cells. Spindle cells showed variable cytoplasmic immunoreactivity for vimentin, smooth muscle actin, and desmin. Round, bi-, and multinucleated giant cells only had marked multifocal cytoplasmic immunoreactivity for vimentin. Neoplastic cells did not have immunoreactivity for cytokeratin, GFAP, S100, and CD117. A final diagnosis of duodenal leiomyosarcoma was made. This article reports the pathologic, cytologic, and immunohistochemical findings of this case and discusses the main differential diagnoses in cases of intestinal sarcomas in domestic animals.
Assuntos
Doenças do Gato , Leiomiossarcoma , Sarcoma , Gatos , Masculino , Animais , Leiomiossarcoma/diagnóstico , Leiomiossarcoma/veterinária , Leiomiossarcoma/patologia , Vimentina , Sarcoma/patologia , Sarcoma/veterinária , Diagnóstico Diferencial , Vômito/veterinária , Doenças do Gato/diagnósticoRESUMO
Eosinophilic myocarditis is a human condition that has been rarely documented in animals. We now report two unrelated porcine cases of idiopathic eosinophilic granulomatous myocarditis that resembled the human disease and which were associated with sudden death. The most relevant gross finding in both cases was marked cardiomegaly, accompanied by raised, multifocal to coalescent small white nodules (1-2 mm) and poorly demarcated multifocal pale areas in the epicardium. Histologically, there were multifocal to coalescent areas of cardiomyocyte loss with replacement by an intense inflammatory infiltrate of eosinophils and epithelioid macrophages, and proliferation of fibrous connective tissue. Immunohistochemistry for porcine circovirus type 2 (PCV2) and Toxoplasma gondii, in-situ hybridization and quantitative polymerase chain reaction tests for PCV2 and porcine circovirus type 3 and aerobic bacterial culture on myocardium samples were negative.
Assuntos
Infecções por Circoviridae , Circovirus , Miocardite , Doenças dos Suínos , Animais , Infecções por Circoviridae/veterinária , DNA Viral/análise , Humanos , Hibridização In Situ/veterinária , Miocardite/complicações , Miocardite/veterinária , Suínos , Doenças dos Suínos/patologiaRESUMO
In collaboration with the American College of Veterinary Pathologists.
Assuntos
Patologia Veterinária , Médicos Veterinários , Animais , Humanos , Estados UnidosRESUMO
Visna-maedi is a multisystemic and progressive inflammatory disease caused by a non-oncogenic retrovirus (Visna-maedi virus, VMV). An outbreak of visna-maedi occurred in Southern Brazil in sheep with clinical signs of blindness and stumbling gait. At post-mortem examination, all animals had similar lesions, including heavy non-collapsed lungs and multifocal yellow areas in the cerebral white matter, affecting mainly the periventricular region. These lesions corresponded histologically to lymphocytic interstitial pneumonia and histiocytic periventricular encephalitis surrounding areas of necrosis, in addition to significant demyelination in the brain. Serology was performed in all the sheep from the flock and 14% were seropositive for VMV. The presence of VMV was confirmed through PCR and partial sequencing of the 5'LTR. Sequencing demonstrated that the virus had 89.7 to 90.0% of nucleotide identity with VMV strains reported in the USA. This is the first description of clinical disease related to VMV in Brazil leading to economic losses. This study calls for the need to implement control measures to prevent the spread of small ruminant lentiviruses in Brazil.
Assuntos
Pneumonia Intersticial Progressiva dos Ovinos , Vírus Visna-Maedi , Visna , Animais , Brasil/epidemiologia , Surtos de Doenças/veterinária , Pneumonia Intersticial Progressiva dos Ovinos/epidemiologia , Pneumonia Intersticial Progressiva dos Ovinos/prevenção & controle , Ovinos , Visna/epidemiologia , Vírus Visna-Maedi/genéticaRESUMO
A 15-year-old captive female Urutu snake (Bothrops alternatus) was presented with anorexia, apathy, dehydration, pale mucous membranes and marked enlargement of the caudal third of the coelomic cavity. Radiographs and ultrasonography were suggestive of faecal impaction or neoplasia. Post-mortem findings consisted of solid faecal impaction of the intestines, secondary to obstruction caused by a rectal tumour. Cytological examination of impression smears of the tumour revealed polygonal to round neoplastic cells arranged in clusters in an abundant extracellular mucinous matrix. Histologically, there was transmural growth of neoplastic epithelial cells arranged in small nests or individually and immersed in abundant extracellular mucin lakes. The neoplastic cells were immunopositive for cytokeratin. Clinical and pathological aspects indicated a diagnosis of mucinous rectal adenocarcinoma.
Assuntos
Adenocarcinoma Mucinoso , Bothrops , Neoplasias Retais , Adenocarcinoma Mucinoso/veterinária , Animais , Animais de Zoológico , Evolução Fatal , Feminino , Neoplasias Retais/veterináriaRESUMO
We describe the pathology of gastrointestinal tuberculosis in three cows. Gross lesions were found in the jejunum and ileum in all cases, and were characterized by button-shaped ulcers in the mucosa overlying Peyer's patches and adjacent mucosa. One case had similar changes in the pyloric region of the abomasum. In the affected intestinal segments, marked granulomatous inflammation effaced the lymphoid follicles of Peyer's patches and often extended transmurally. Transmural granulomatous inflammation was also found in the abomasum of one cow. Acid-fast bacilli were identified by the Ziehl-Neelsen method and the lesions were immunolabelled by an anti-Mycobacterium tuberculosis complex antibody. M. tuberculosis var. bovis was isolated in one case. Disseminated tuberculosis and marked lung lesions in all cows suggested that the gastrointestinal lesions were most likely secondary to pulmonary infection.
Assuntos
Doenças dos Bovinos , Mycobacterium tuberculosis , Tuberculose Gastrointestinal , Animais , Bovinos , Doenças dos Bovinos/patologia , Feminino , Granuloma/patologia , Granuloma/veterinária , Intestinos/patologia , Tuberculose Gastrointestinal/patologia , Tuberculose Gastrointestinal/veterináriaRESUMO
Fatal hypothermia represents a diagnostic challenge in veterinary pathology. Wischnewski spots (WS) and black oesophagus (BO) have been described in human cases of fatal hypothermia but rarely in animals. We now describe WS and BO in suspected fatal hypothermia in a free-ranging brown howler monkey (Alouatta guariba clamitans) and a pet rabbit (Oryctolagus cuniculus). Both animals had dark spots resembling WS on the gastric mucosa and the monkey also had BO with haematin deposition. In both cases, stress factors and relatively cold environmental conditions were present prior to death.
Assuntos
Alouatta , Hipotermia , Coelhos , Animais , Esôfago/patologia , Evolução Fatal , Hipotermia/veterináriaRESUMO
Pantoea agglomerans, family Enterobacteriaceae, is a Gram-negative bacterium that may be isolated from soil and from plants. This bacterium has been associated with disease in plants, humans, and rarely in domestic animal species. We describe here a case of fibrinonecrotic placentitis and equine abortion associated with P. agglomerans infection in southern Brazil. A fetus with 10 months of gestation and its placenta were evaluated. Gross lesions were observed in the cervical star extending to the body of the chorioallantois and consisted of a focally extensive, transmural, severely thickened yellow area. Histologically, this area in the chorioallantois was effaced by severe necrosis, associated with marked inflammatory infiltrate of neutrophils and abundant deposition of fibrin and cellular debris. Aggregates of bacterial rods were noted intermixed with inflammation areas. No significant lesions were observed in the remaining organs inspected. Tissue samples of the lung, placenta, and stomach contents were cultured, and microbiological tests revealed the growth of P. agglomerans in all evaluated samples. The present study reaffirms the participation of P. agglomerans as a cause of bacterial placentitis and abortion in horses.