RESUMO
By observing the actions taken by operators, it is possible to determine the risk level of a work task. One method for achieving this is the recognition of human activity using biosignals and inertial measurements provided to a machine learning algorithm performing such recognition. The aim of this research is to propose a method to automatically recognize physical exertion and reduce noise as much as possible towards the automation of the Job Strain Index (JSI) assessment by using a motion capture wearable device (MindRove armband) and training a quadratic support vector machine (QSVM) model, which is responsible for predicting the exertion depending on the patterns identified. The highest accuracy of the QSVM model was 95.7%, which was achieved by filtering the data, removing outliers and offsets, and performing zero calibration; in addition, EMG signals were normalized. It was determined that, given the job strain index's purpose, physical exertion detection is crucial to computing its intensity in future work.
Assuntos
Ergonomia , Esforço Físico , Humanos , Eletromiografia/métodos , Ergonomia/métodos , Algoritmos , Aprendizado de MáquinaRESUMO
Decoupling growth from product synthesis is a promising strategy to increase carbon partitioning and maximize productivity in cell factories. However, reduction in both substrate uptake rate and metabolic activity in the production phase are an underlying problem for upscaling. Here, we used CRISPR interference to repress growth in lactate-producing Synechocystis sp. PCC 6803. Carbon partitioning to lactate in the production phase exceeded 90%, but CO2 uptake was severely reduced compared to uptake during the growth phase. We characterized strains during the onset of growth arrest using transcriptomics and proteomics. Multiple genes involved in ATP homeostasis were regulated once growth was inhibited, which suggests an alteration of energy charge that may lead to reduced substrate uptake. In order to overcome the reduced metabolic activity and take advantage of increased carbon partitioning, we tested a novel production strategy that involved alternating growth arrest and recovery by periodic addition of an inducer molecule to activate CRISPRi. Using this strategy, we maintained lactate biosynthesis in Synechocystis for 30 days in a constant light turbidostat cultivation. Cumulative lactate titers were also increased by 100% compared to a constant growth-arrest regime, and reached 1 g/L. Further, the cultivation produced lactate for 30 days, compared to 20 days for the non-growth arrest cultivation. Periodic growth arrest could be applicable for other products, and in cyanobacteria, could be linked to internal circadian rhythms that persist in constant light.
Assuntos
Ácido Láctico , Synechocystis , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Synechocystis/genéticaRESUMO
Energy harvesting shock absorbers (EHSA) have made great progress in recent years, although there are still no commercial solutions for this technology. This paper addresses the question of whether, and under what conditions, an EHSA can completely replace a conventional one. In this way, any conventional suspension could be replicated at will, while recovering part of the wasted energy. This paper focuses on mimicking the original passive damper behavior by continuously varying the electrical parameters of the regenerative damper. For this study, a typical ball-screw EHSA is chosen, and its equivalent suspension parameters are tried to be matched to the initial damper. The methodology proposes several electrical control circuits that optimize the dynamic behavior of the regenerative damper from the continuous variation of a load resistance. The results show that, given a target damper curve, the regenerative damper can adequately replicate it when there is a minimum velocity in the damper. However, when the damper velocity is close to zero, the only way to compensate for inertia is through the introduction of external energy to the system.
Assuntos
RotaçãoRESUMO
The number of high-resolution structures of protein complexes obtained using cryo-electron microscopy (cryo-EM) is increasing rapidly. Cryo-EM maps of large macromolecular complexes frequently contain regions resolved at different resolution levels, and modeling atomic structures de novo can be difficult for domains determined at worse than 5 Å in the absence of atomic information from other structures. Here we describe the details and step-by-step decisions in the strategy we followed to model the RUVBL2-binding domain (RBD), a 14 kDa domain at the C-terminus of RNA Polymerase II associated protein 3 (RPAP3) for which atomic information was not available. Modeling was performed on a cryo-EM map at 4.0-5.5 Å resolution, integrating information from secondary structure predictions, homology modeling, restraints from cross-linked mass spectrometry, and molecular dynamics (MD) in AMBER. Here, we compare our model with the structure of RBD determined by NMR to evaluate our strategy. We also perform new MD simulations to describe important residues mediating the interaction of RBD with RUVBL2 and analyze their conservation in RBD homologous domains. Our approach and its evaluation can serve as an example to address the analysis of medium resolution regions in cryo-EM maps.
Assuntos
Simulação de Dinâmica Molecular , Proteínas , Microscopia Crioeletrônica , Substâncias Macromoleculares , Conformação Proteica , Estrutura Secundária de ProteínaRESUMO
A sustainable society will have to largely refrain from the use of fossil carbon deposits. In such a regime, renewable electricity can be harvested as a primary source of energy. However, as for the synthesis of carbon-based materials from bulk chemicals, an alternative is required. A sustainable approach towards this is the synthesis of commodity chemicals from CO2 , water and sunlight. Multiple paths to achieve this have been designed and tested in the domains of chemistry and biology. In the latter, the use of both chemotrophic and phototrophic organisms has been advocated. 'Direct conversion' of CO2 and H2 O, catalyzed by an oxyphototroph, has excellent prospects to become the most economically competitive of these transformations, because of the relative ease of scale-up of this process. Significantly, for a wide range of energy and commodity products, a proof of principle via engineering of the corresponding production organism has been provided. In the optimization of a cyanobacterial production organism, a wide range of aspects has to be addressed. Of these, here we will put our focus on: (1) optimizing the (carbon) flux to the desired product; (2) increasing the genetic stability of the producing organism and (3) maximizing its energy conversion efficiency. Significant advances have been made on all these three aspects during the past 2 years and these will be discussed: (1) increasing the carbon partitioning to >50%; (2) aligning product formation with the growth of the cells and (3) expanding the photosynthetically active radiation region for oxygenic photosynthesis.
Assuntos
Cianobactérias/metabolismo , Oxigênio/metabolismo , Fotossíntese/fisiologia , Dióxido de Carbono/metabolismo , Água/metabolismoRESUMO
OBJECTIVE: To determine the prevalence and associated factors of attempted suicide (AS) in a sample of Mexican adolescents between 10 and 19 years of age. METHODS: A cross-sectional study was conducted with data from the 2012 National Health and Nutrition Survey (ENSANUT 2012 by its initials in Spanish). Based on multi-stage stratified probability sampling, ENSANUT 2012 canvassed 50 528 households including 21 519 adolescents. A logistic regression model was used to identify the factors associated with AS. The model was selected based on the maximum likelihood test. RESULTS: Lifetime prevalence of AS amounted to 2.7% and was heterogeneously distributed across Mexico. Women showed significantly higher risk for AS than men (ORA(adjusted OR)) 6.3, 95% confidence interval CI 4.94 to 8.06), and individuals who experienced damage to health caused by violence over the last 12 months were 4.35 times (ORA 4.35, 95% CI 3.27 to 5.80) more likely to engage in AS than those who did not. The likelihood of engaging in AS was 1.5 times higher (ORA1.5 (95% CI 1.12 to 2.01)) among subjects with mild and 4.22 times higher (95% CI 3.21 to 5.54) among subjects with moderate eating disorders compared to those with no eating disorders. Other AS-associated factors included age, smoking, consuming alcohol and living with relatives other than parents. CONCLUSIONS: Awareness of the risk factors associated with AS can help identify the populations who are exposed to injury or death by suicide. This can orient the actions of the health sector towards those who are most vulnerable. Actions must be matched by more qualitative and quantitative research on the subject.
Assuntos
Vítimas de Crime/psicologia , Transtornos da Alimentação e da Ingestão de Alimentos/psicologia , Tentativa de Suicídio/estatística & dados numéricos , Uso de Tabaco/epidemiologia , Consumo de Álcool por Menores/estatística & dados numéricos , Adolescente , Criança , Vítimas de Crime/estatística & dados numéricos , Estudos Transversais , Transtornos da Alimentação e da Ingestão de Alimentos/epidemiologia , Feminino , Humanos , Masculino , México/epidemiologia , Prevalência , Fatores de Risco , Tentativa de Suicídio/psicologia , Uso de Tabaco/psicologia , Consumo de Álcool por Menores/psicologia , ViolênciaRESUMO
Cellular stability, assembly and activation of a growing list of macromolecular complexes require the action of HSP90 working in concert with the R2TP/Prefoldin-like (R2TP/PFDL) co-chaperone. RNA polymerase II, snoRNPs and complexes of PI3-kinase-like kinases, a family that includes the ATM, ATR, DNA-PKcs, TRAPP, SMG1 and mTOR proteins, are among the clients of the HSP90-R2TP system. Evidence links the R2TP/PFDL pathway with cancer, most likely because of the essential role in pathways commonly deregulated in cancer. R2TP forms the core of the co-cochaperone and orchestrates the recruitment of HSP90 and clients, whereas prefoldin and additional prefoldin-like proteins, including URI, associate with R2TP, but their function is still unclear. The mechanism by which R2TP/PFLD facilitates assembly and activation of such a variety of macromolecular complexes is poorly understood. Recent efforts in the structural characterization of R2TP have started to provide some mechanistic insights. We summarize recent structural findings, particularly how cryo-electron microscopy (cryo-EM) is contributing to our understanding of the architecture of the R2TP core complex. Structural differences discovered between yeast and human R2TP reveal unanticipated complexities of the metazoan R2TP complex, and opens new and interesting questions about how R2TP/PFLD works.
Assuntos
Chaperonas Moleculares/química , Animais , Microscopia Crioeletrônica , Proteínas de Choque Térmico HSP90/química , Humanos , Neoplasias , Saccharomyces cerevisiae , Proteínas de Saccharomyces cerevisiaeRESUMO
There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = -47.909 + (1.176xCL1) - (0.00063968xCL1²). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
Assuntos
Iohexol/análise , Testes de Função Renal/métodos , Plasma/metabolismo , Animais , Calibragem , Iohexol/farmacocinética , Testes de Função Renal/normas , Controle de Qualidade , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , SuínosRESUMO
RuvBL1 and RuvBL2, also known as Pontin and Reptin, are AAA+ proteins essential in small nucleolar ribonucloprotein biogenesis, chromatin remodelling, nonsense-mediated messenger RNA decay and telomerase assembly, among other functions. They are homologous to prokaryotic RuvB, forming single- and double-hexameric rings; however, a DNA binding domain II (DII) is inserted within the AAA+ core. Despite their biological significance, questions remain regarding their structure. Here, we report cryo-electron microscopy structures of human double-ring RuvBL1-RuvBL2 complexes at â¼15 Å resolution. Significantly, we resolve two coexisting conformations, compact and stretched, by image classification techniques. Movements in DII domains drive these conformational transitions, extending the complex and regulating the exposure of DNA binding regions. DII domains connect with the AAA+ core and bind nucleic acids, suggesting that these conformational changes could impact the regulation of RuvBL1-RuvBL2 containing complexes. These findings resolve some of the controversies in the structure of RuvBL1-RuvBL2 by revealing a mechanism that extends the complex by adjustments in DII.
Assuntos
Proteínas de Transporte/química , DNA Helicases/química , Proteínas de Ligação a DNA/química , ATPases Associadas a Diversas Atividades Celulares , Proteínas de Transporte/metabolismo , Proteínas de Transporte/ultraestrutura , Microscopia Crioeletrônica , DNA Helicases/metabolismo , DNA Helicases/ultraestrutura , Proteínas de Ligação a DNA/ultraestrutura , Humanos , Modelos Moleculares , Conformação Proteica , Estrutura Terciária de ProteínaRESUMO
Introduction: In the 2022-23 Mpox outbreak, cases also occurred in children, adolescents, and adults aged 50 years and older, for whom the risk of transmission is low and whose epidemiological characteristics are less known, compared to high-risk groups such as young adults. Here we describe the epidemiological characteristics of Mpox in children, adolescents and adults aged 50 years and older in the global Mpox outbreak. Methods: A retrospective study on laboratory-confirmed surveillance data of Mpox cases reported to World Health Organization (WHO) was conducted. Case data from WHO's 2022-23 Mpox Outbreak: Global Trends from 1 January 2022 to 1 September 2023 was used for our analysis. We included cases reported by WHO with data on age (children [range, 0 to 9 years], adolescents [range, 10 to 17 years], adults 50 to 59 years, and adults 60 years and older), gender, WHO region, hospital admission, and intensive care unit admission. Results: Until September 01, 2023, data from 89,752 cases of Mpox have been reported to WHO. Of all the reported cases, 1124 (1.3%), 6296 (7.0%) and 1501 (1.6%) were children and adolescents, adults aged 50-59 years, and adults aged 60 years or older, respectively, and the proportion varied among WHO regions. There was a high proportion of cases among population aged 0-17 years, adolescents (256 [66.3%]) from the region of the Americas and girls aged 0-9 years [127 (46.7%)] from the African region. Men aged 50-59 years (3495 [57.2%] vs. 2553 [41.8%] cases from the region of the Americas and the European region, respectively) and men aged 60-69 years (639 [60.0%] vs. 607 [48.4%] from the region of the Americas and the European region) were most affected, compared to other age groups and women. Among children, adolescents, and adults aged 50 years or older, a low proportion of cases developed some complications and required hospital admission, and some cases were admitted to the intensive care unit. Conclusions: Epidemiological evidence of Mpox in these low-risk groups highlights the risk of wider community transmission. Therefore, while efforts continue to control the global outbreak of Mpox in high-risk groups, it is also necessary to ensure that these low-risk groups have access to timely health care and vaccination.
RESUMO
BACKGROUND: In Latin America, Peru has the second highest number of cases of monkeypox (Mpox), of which more than 50 % are Human Immunodeficiency Virus (HIV)-positive. Here, we compared the epidemiological and clinical characteristics of Mpox between people with and without HIV in Peru. METHODS: We conducted a national retrospective study using data on confirmed cases of Mpox reported by the Peruvian National Surveillance System from 15 June 2022 to 31 December 2023. RESULTS: A total of 3561 confirmed cases of Mpox were included. Of these, 2123 (60 %) patients were people living with HIV (PLWH), with increased odds for those aged 30 years or older, homosexual (adjusted odds ratio [aOR] 8.58 [6.95-10.59], p<0.0001), bisexual (aOR=4.44 [3.46-5.69], p<0.0001), sex workers (aOR=2.24 [1.07-4.68], p=0.032), people with a history of syphilis (aOR=2.07 [1.66-2.58], p<0.001), and hospitalized (aOR=3.08 [2.03-4.68], p<0.001). PLWH were more likely to have proctitis (aOR=1.73 [1.26-2.37], p=0.001). The overall mortality was 20 of 3561 (0.56 %). Among PLWH and Mpox, more deaths occurred in hospitalized (p<0.001) and non-ART (p<0.001) individuals. CONCLUSION: Our findings highlight that HIV infection among Mpox cases in Peru is associated with high-risk sexual behaviour and a high likelihood of hospitalization.
Assuntos
Infecções por HIV , Mpox , Humanos , Peru/epidemiologia , Masculino , Feminino , Adulto , Infecções por HIV/epidemiologia , Infecções por HIV/complicações , Estudos Retrospectivos , Pessoa de Meia-Idade , Adulto Jovem , Mpox/epidemiologia , Adolescente , Fatores de Risco , Coinfecção/epidemiologia , Sífilis/epidemiologia , Sífilis/complicaçõesRESUMO
INTRODUCTION: Recent outbreaks of monkeypox (Mpox) have occurred in countries outside of Africa, with large numbers of cases spreading rapidly to almost every continent. We aimed to analyze the correlation between the Global Health Security (GHS) Index (categories and indicators) and the Mpox case rate in different regions and globally. METHODS: In this cross-sectional study, we used data from Mpox cases from the WHO, and the GHS categories and indicators for detection, prevention, reporting, health system, rapid response, international norms compliance, and risk environment. Outcome measures were the relationship between GHS index (categories and indicators) and Mpox case rate using crude and adjusted non-linear regression models. RESULTS: After performing adjusted analyses, only risk environment and detection and reporting index were associated with Mpox case rates in the 99 countries and the Region of the Americas, respectively. Antimicrobial resistance (AMR) indicators of the prevention category, risk communication of the rapid response category, the joint external evaluation and performance veterinary services of the norms category, and the infrastructure adequacy of the risk environment category were associated with Mpox case rates in the 99 countries (p<0.05). Laboratory systems strength and quality indicator of the detection and reporting category, and emergency response operation indicator of the response rapid category were associated with Mpox case rates in the countries of the region of the Americas (p<0.05). AMR indicator of the prevention of the emergence category, and the infrastructure adequacy of the risk environment category were associated with Mpox case rates in the countries of the European Region (p<0.05). In the countries of the other regions, only the trade and travel restrictions indicator of the rapid response category was associated with Mpox case rates (p<0.05). CONCLUSIONS: Countries, particularly in the Americas region, with high levels of infrastructure adequacy and laboratory system strength and quality as measured by the GHS index are better equipped to detect more Mpox cases. Therefore, they have higher Mpox case detection rates and can successfully respond to Mpox outbreaks.
Assuntos
Saúde Global , Mpox , Estudos Transversais , Mpox/epidemiologia , Mpox/diagnóstico , Humanos , Surtos de Doenças/prevenção & controle , Organização Mundial da SaúdeRESUMO
Temporary immersion systems (TIS) have been widely recognized as a promising technology for micropropagation of various plant species. The TIS provides a suitable environment for culture and allows intermittent contact of the explant with the culture medium at different immersion frequencies and aeration of the culture in each cycle. The frequency or immersion is one of the most critical parameters for the efficiency of these systems. The design, media volume, and container capacity substantially improve cultivation efficiency. Different TIS have been developed and successfully applied to micropropagation in various in vitro systems, such as sprout proliferation, microcuttings, and somatic embryos. TIS increases multiplication and conversion rates to plants and a better response during the ex vitro acclimatization phase. This article covers the use of different immersion systems and their applications in plant biotechnology, particularly in plant tissue culture, as well as its use in the massive propagation of plants of agroeconomic interest.
Assuntos
Aclimatação , Desenvolvimento Vegetal , Meios de Cultura/química , Técnicas de Cultura de Tecidos/métodos , Técnicas de Cultura de Tecidos/instrumentação , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/fisiologia , Plantas , Imersão , Técnicas de Embriogênese Somática de Plantas/métodosRESUMO
Somatic embryogenesis (SE) is a clear example of cellular totipotency. The SE of the genus Coffea has become a model for in vitro propagation for woody species and for the large-scale production of disease-free plants that provide an advantage for modern agriculture. Temporary immersion systems (TIS) are in high demand for the propagation of plants. The success of this type of bioreactor is based on the alternating cycles of immersion of the plant material in the culture medium, usually a few minutes, and the permanence outside the medium of the tissues for several hours. Some bioreactors are very efficient for propagating one species but not another. The efficiency of bioreactors depends on the species, the tissue used to propagate, the species' nutritional needs, the amount of ethylene produced by the tissue, and many more. In this protocol, we show how we produce C. canephora plants that are being taken to the field.
Assuntos
Coffea , Técnicas de Embriogênese Somática de Plantas , Técnicas de Embriogênese Somática de Plantas/métodos , Coffea/crescimento & desenvolvimento , Coffea/genética , Reatores Biológicos , Sementes/crescimento & desenvolvimento , Meios de Cultura/químicaRESUMO
Agrobacterium's journey has been a roller coaster, from being a pathogen to becoming a powerful biotechnological tool. While A. tumefaciens has provided the scientific community with a versatile tool for plant transformation, Agrobacterium rhizogenes has given researchers a Swiss army knife for developing many applications. These applications range from a methodology to regenerate plants, often recalcitrant, to establish bioremediation protocols to a valuable system to produce secondary metabolites. This chapter reviews its discovery, biology, controversies over its nomenclature, and some of the multiple applications developed using A. rhizogenes as a platform.
Assuntos
Agrobacterium , Biotecnologia , Agrobacterium/genética , Biotecnologia/métodos , Transformação Genética , História do Século XX , História do Século XXI , Plantas Geneticamente Modificadas/genética , Plantas/microbiologia , Plantas/genéticaRESUMO
Objective: To determine the factors associated with left ventricular diastolic dysfunction (LVDD) in adults residing in a region of the Andes in Peru. Method: A case-control study was conducted on adults living at an altitude of more than 3000 meters in Peru. Cases consisted of patients diagnosed with LVDD through echocardiography, whereas controls were adults without LVDD, as confirmed by echocardiography. Results: A total of 50 cases and 100 controls were included in the study. Among them, 38.7% had high blood pressure, and 41.3% were overweight. Upon adjusted analysis, age 60 or older (aOR: 4.06; 95%CI: 1.29-12.8), female sex (aOR: 2.24; 95%CI: 1.01-4.96) and left ventricular hypertrophy (aOR: 3.17; 95%CI: 1.41-7.17) were identified as statistically significant factors associated with LVDD. Conclusions: The risk of LVDD is associated with older adults, female gender, and left ventricular hypertrophy among individuals residing above 3000 meters altitude in a region of the Andes, in Peru.
Objetivo: Determinar los factores asociados con la disfunción diastólica del ventrículo izquierdo (DDVI) en adultos de una región de los Andes, en Perú. Método: Estudio de casos y controles en adultos residentes a más de 3000 metros de altitud en Perú. Los casos fueron pacientes adultos diagnosticados con DDVI por ecocardiografía, y los controles fueron adultos sin DDVI por ecocardiografía. Resultados: Se incluyeron 50 casos y 100 controles. El 38.7% tuvieron hipertensión arterial y el 41.3% sobrepeso. En el análisis ajustado, la edad de 60 o más años (ORa: 4.06; IC95%: 1.29-12.8), el sexo femenino (ORa: 2.24; IC95%: 1.01-4.96) y la hipertrofia ventricular izquierda (ORa: 3.17; IC95%: 1.41-7.17) fueron factores estadísticamente significativos. Conclusiones: El riesgo de DDVI estuvo asociado a los adultos mayores, las mujeres y los pacientes con hipertrofia ventricular izquierda que viven por encima de los 3000 metros de altitud en una región de los Andes, en Perú.
RESUMO
Numerous papers report the efficiency of the automatic interpretation capabilities of commercial algorithms. Unfortunately, these algorithms are proprietary, and academia has no means of directly contributing to these results. In fact, nothing at the same stage of development exists in academia. Despite the extensive research in ECG signal processing, from signal conditioning to expert systems, a cohesive single application for clinical use is not ready yet. This is due to a serious lack of coordination in the academic efforts, which involve not only algorithms for signal processing, but also the signal acquisition equipment itself. For instance, the different sampling rates and the different noise levels frequently found in the available signal databases can cause severe incompatibility problems when the integration of different algorithms is desired. Therefore, this work aims to solve this incompatibility problem by providing the academic community with a diagnostic-grade electrocardiograph. The intention is to create a new standardized ECG signals database in order to address the automatic interpretation problem and create an electrocardiography system that can fully assist clinical practitioners, as the proprietary systems do. Achieving this objective is expected through an open and coordinated collaboration platform for which a webpage has already been created.
RESUMO
A New Adaptive Self-Tuning Fourier Coefficients Algorithm for Periodic Torque Ripple Minimization in Permanent Magnet Synchronous Motors (PMSM) Torque ripple occurs in Permanent Magnet Synchronous Motors (PMSMs) due to the non-sinusoidal flux density distribution around the air-gap and variable magnetic reluctance of the air-gap due to the stator slots distribution. These torque ripples change periodically with rotor position and are apparent as speed variations, which degrade the PMSM drive performance, particularly at low speeds, because of low inertial filtering. In this paper, a new self-tuning algorithm is developed for determining the Fourier Series Controller coefficients with the aim of reducing the torque ripple in a PMSM, thus allowing for a smoother operation. This algorithm adjusts the controller parameters based on the component's harmonic distortion in time domain of the compensation signal. Experimental evaluation is performed on a DSP-controlled PMSM evaluation platform. Test results obtained validate the effectiveness of the proposed self-tuning algorithm, with the Fourier series expansion scheme, in reducing the torque ripple.
Assuntos
Análise de Fourier , Software , Torque , Algoritmos , Simulação por Computador , Humanos , ImãsRESUMO
Park et al.'s (2022) goal of bringing conceptual clarity to the study of psychological aspects of well-being is a good one. We consider their work in terms of its implications for moving towards an understanding of well-being that reflects the full spectrum of human experience, especially the experience of people who remain underrepresented, and poorly accounted for, in psychological science. In our view, there is reason to think that strengthening existing frameworks and broadening in terms of methodologies will be most productive for developing a comprehensive and inclusive understanding of well-being. We describe the distinct strength of the subjective well-being (SWB) construct for this purpose and offer two empirical examples that highlight the value of multiple measures and methods for understanding well-being. We suggest that continued use of the SWB measure, combined with state-of-the-art emotion measurement, and a mix of qualitative and quantitative methodologies be recommended as the way forward.