RESUMO
The objective of this study was to compare two different rice simulation models--standalone (Decision Support System for Agrotechnology Transfer [DSSAT]) and web based (SImulation Model for RIce-Weather relations [SIMRIW])--with agrometeorological data and agronomic parameters for estimation of rice crop production in southern semi-arid tropics of India. Studies were carried out on the BPT5204 rice variety to evaluate two crop simulation models. Long-term experiments were conducted in a research farm of Acharya N G Ranga Agricultural University (ANGRAU), Hyderabad, India. Initially, the results were obtained using 4 years (1994-1997) of data with weather parameters from a local weather station to evaluate DSSAT simulated results with observed values. Linear regression models used for the purpose showed a close relationship between DSSAT and observed yield. Subsequently, yield comparisons were also carried out with SIMRIW and DSSAT, and validated with actual observed values. Realizing the correlation coefficient values of SIMRIW simulation values in acceptable limits, further rice experiments in monsoon (Kharif) and post-monsoon (Rabi) agricultural seasons (2009, 2010 and 2011) were carried out with a location-specific distributed sensor network system. These proximal systems help to simulate dry weight, leaf area index and potential yield by the Java based SIMRIW on a daily/weekly/monthly/seasonal basis. These dynamic parameters are useful to the farming community for necessary decision making in a ubiquitous manner. However, SIMRIW requires fine tuning for better results/decision making.
Assuntos
Modelos Teóricos , Oryza/crescimento & desenvolvimento , Tempo (Meteorologia) , Índia , Folhas de Planta/crescimento & desenvolvimentoRESUMO
In neurons of dorsal motor nucleus of the vagus that is involved in the gastric motility and possibly emesis, application of 5-hydroxytryptamine produces membrane depolarization, and suppresses spike-repolarization and spike-afterhyperpolarization, suggesting divergent effects of 5-hydroxytryptamine through activating multiple subtypes of 5-hydroxytryptamine receptors. However, only the role of 5-hydroxytryptamine 2A receptors has been established to be responsible for the depolarization, and the mechanisms underlying the modulation of spikes remain unknown although a role of 5-hydroxytryptamine 4 receptors was implicated in modulations of spikes. There is now increasing evidence for the role of 5-hydroxytryptamine receptors in neurons involved in generating emesis following administration of anticancer drug. Since antagonists of 5-hydroxytryptamine 3/4 receptors are widely used as anti-emetic drugs, we have reevaluated the functional roles of 5-hydroxytryptamine 3/4 receptors of dorsal motor nucleus of the vagus neurons, especially in modulating transient outward currents that are presumed to be involved in spike-repolarization and spike-afterhyperpolarization. Whole-cell patch-clamp recordings were made from the dorsal motor nucleus of the vagus neurons, which were identified by a retrograde tracing method with dextran-tetramethylrhodamine-lysine injected into a bundle of abdominal vagus nerves. Under a voltage-clamp condition, dorsal motor nucleus of the vagus neurons expressed a prominent A-like current. The activation of 5-hydroxytryptamine 3 receptors reversibly increased the resting membrane conductance while the activation of 5-hydroxytryptamine 4 receptors led to an almost irreversible decrease in the A-like current. A long-lasting suppression of A-like current by transient activation of 5-hydroxytryptamine 4 receptors would result in a long-lasting increase in the excitability of dorsal motor nucleus of the vagus neurons, which might be involved in generation of the long-lasting facilitation of gastric motility or in generation of the long-lasting gastric relaxation through the activation of enteric non-adrenergic non-cholinergic neurons as implicated in the delayed emesis induced by anticancer drugs.
Assuntos
Bulbo/citologia , Neurônios/fisiologia , Receptores 5-HT3 de Serotonina/fisiologia , Receptores 5-HT4 de Serotonina/fisiologia , Nervo Vago/fisiologia , Ácido 4-Aminobenzoico/farmacologia , 5-Metoxitriptamina/farmacologia , Animais , Animais Recém-Nascidos , Cálcio/farmacologia , Relação Dose-Resposta a Droga , Interações Medicamentosas , Granisetron/farmacologia , Técnicas In Vitro , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Potenciais da Membrana/efeitos da radiação , Técnicas de Patch-Clamp/métodos , Ratos , Ratos Wistar , Serotonina/análogos & derivados , Serotonina/farmacologia , Antagonistas da Serotonina/farmacologia , Agonistas do Receptor de Serotonina/farmacologia , para-AminobenzoatosRESUMO
A single dose of isatin (indole-2,3-dione)(i.p.), an endogenous MAO inhibitor, significantly increased norepinephrine and 5-hydroxytryptamine concentrations in the rat brain and also significantly increased acetylcholine and dopamine (DA) levels in the rat striatum. Urinary isatin concentrations in patients with Parkinson's disease tend to increase according to the severity of disease. We have developed a rat model of Parkinson's disease induced by the Japanese encephalitis virus (JEV). The distribution of the pathological lesions of JEV-rats resemble those found in Parkinson's disease. Significant behavioral improvement was observed in JEV-rats after isatin, L-DOPA and selegiline administration using a pole test. Both isatin and selegiline prevented the decrease in striatum DA levels of JEV-rats. The increased turnover of DA (DOPAC/DA) induced by JEV was significantly inhibited by isatin, but not selegiline. These findings suggest that JEV-infected rats may serve as a model of Parkinson's disease and that exogenously administered isatin and selegiline can improve JEV-induced parkinsonism by increasing DA concentrations in the striatum.
Assuntos
Vírus da Encefalite Japonesa (Espécie)/patogenicidade , Isatina/uso terapêutico , Inibidores da Monoaminoxidase/uso terapêutico , Doença de Parkinson , Animais , Animais Recém-Nascidos , Modelos Animais de Doenças , Atividade Motora/efeitos dos fármacos , Atividade Motora/fisiologia , Doença de Parkinson/tratamento farmacológico , Doença de Parkinson/etiologia , Doença de Parkinson/virologia , Ratos , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
The regulating mechanisms of PAF-acether (platelet-activating factor) biosynthesis in cultured human vascular endothelial cells stimulated with thrombin were investigated. The formation of PAF-acether was maximal at 5 min after stimulation and gradually decreased for up to 30 min. Thrombin induced a rapid 3-4-fold increase in the activity which was maximal by 1 min after stimulation and returned progressively to basal level within 10 min. The thrombin-induced enhancement in acetyltransferase activity was due to an increase of the Vmax of the acetylation reaction without a significant effect on the apparent Km of the enzyme for acetyl-CoA. Human endothelial cells also exhibited a basal PAF-acether acetylhydrolase activity which was not altered upon thrombin stimulation. The pretreatment with 2 mM phenylmethylsulfonyl fluoride (PMSF), a serine proteinase inhibitor reported to block the acetylhydrolase, induced about 2-times more PAF-acether production in response to 2.5 U/ml thrombin stimulation. However, this enhancement of PAF-acether formation seems to be not only due to the inhibition of the acetylhydrolase, but also to the influences on the activities of the acetyltransferase and other enzymes such as phospholipase A2. These results suggest a key role for acetyltransferase and acetylhydrolase in the regulation of PAF-acether formation and catabolism in thrombin-stimulated human endothelial cells.
Assuntos
Endotélio Vascular/metabolismo , Fator de Ativação de Plaquetas/biossíntese , Trombina/farmacologia , 1-Alquil-2-acetilglicerofosfocolina Esterase , Acetil-CoA C-Acetiltransferase/metabolismo , Ácido Araquidônico , Ácidos Araquidônicos/metabolismo , Células Cultivadas , Endotélio Vascular/efeitos dos fármacos , Feminino , Humanos , Cinética , Fosfolipases A/metabolismo , Fosfolipases A2 , Veias Umbilicais/metabolismoRESUMO
Activin, a member of the transforming growth factor-beta (TGF-beta) superfamily, is present in the bone matrix and assumed to be involved in the regulation of bone formation. In the present study, we investigated whether the release of activin from bone is coupled with bone resorption. Neonatal mouse calvaria were cultured in the presence of various stimulators of bone resorption (parathyroid hormone [PTH], interleukin-1beta, prostaglandin E2) for up to 72 h, and the activin activity in the medium was measured using a specific bioassay for activin. Activin activity was accumulated in proportion to the time- and dose-dependent increase in calcium release from bone into the medium (bone resorption). An inhibition of PTH-dependent bone resorption by a bisphosphonate, disodium dichlormethane-1,1-bisphosphonic acid (Cl2MBP), completely blocked release of activin activity from bone into the medium. In primary culture of calvarial cells, however, neither PTH nor Cl2MBP affected activin production. These findings indicate that release of activin activity from bone tissue is strongly coupled to bone resorption. Because activin possesses osteogenic activities, activin released locally from bone might be involved in the regulation of bone formation in the physiological process of bone remodeling, as has been suggested for TGF-beta.
Assuntos
Reabsorção Óssea , Osso e Ossos/metabolismo , Inibinas/metabolismo , Ativinas , Animais , Animais Recém-Nascidos , Células CHO , Cromatografia Líquida de Alta Pressão , Cricetinae , Meios de Cultura , Dinoprostona/metabolismo , Difosfonatos/farmacologia , Interleucina-1/metabolismo , Camundongos , Técnicas de Cultura de Órgãos , Ratos , Ratos Sprague-DawleyRESUMO
1. The mechanism of polymorphonuclear leukocyte (PMN)-dependent platelet adhesion to cultured endothelial cells induced by platelet-activating factor (PAF) was investigated to determine whether PMNs release or generate any factor(s) capable of inducing platelet adhesion, and the roles of prostacyclin and endothelium-derived relaxing factor (EDRF). 2. Cell-free supernatants, sonicates or rapid filtrates of PAF-stimulated PMN suspensions did not induce platelet adhesion to endothelial cells, but the PMN sonicates induced platelet adhesion when endothelial cells were pretreated with both aspirin and NG-nitro-L-arginine (L-NOARG). Its microphotograph showed that mainly platelet aggregates adhered to the endothelial cell surface. 3. Platelet adhesion induced by the PMN sonicates to aspirin- and L-NOARG-pretreated endothelial cells was dose-dependently prevented by OP-41483 (1-100 nM), and slightly by L-arginine (1 mM). The inhibition of platelet adhesion by OP-41483 and L-arginine was potentiated by their combination. 4. WEB 2170 (3 microM), a PAF antagonist, inhibited platelet adhesion induced by the PMN sonicates. However, PAF alone did not induce significant platelet adhesion to aspirin- and L-NOARG-treated endothelial cells. 5. Platelet adhesion induced by the PMN sonicates was not suppressed by AA-861 and indomethacin. However, both superoxide dismutase and catalase significantly inhibited platelet adhesion, and, in combination, their inhibitory effect was synergistically potentiated. Mannitol had no effect. It was also significantly inhibited by alpha 1-antitrypsin, whereas chymostatin and elastatinal had no effect. 6. PAF-induced platelet adhesion to endothelial cells in the presence of intact PMNs was not suppressed by indomethacin and AA-861, or by protease inhibitors. SOD alone, and in combination with catalase, caused a slight but significant inhibition, while catalase and mannitol by themselves had no effect.7. PMN-induced platelet adhesion was slightly inhibited by OP-41483 (100 nM). L-Arginine (1 mM)alone had no effect, but slightly potentiated the effect of OP-41483. This platelet adhesion was not accompanied by suppression of prostacyclin synthesis.8. The results with the PMN sonicates show that prostacyclin, EDRF and active oxygens are important modulators of intercellular interactions between platelets and endothelial cells. These results further suggest that the mechanism of intact PMN-dependent platelet adhesion is primarily through platelet endothelial cell interactions in which leukocyte-derived active oxygens play a role, but does not involve platelet-platelet interactions inhibitable by prostacyclin.
Assuntos
Epoprostenol/fisiologia , Neutrófilos/fisiologia , Óxido Nítrico/fisiologia , Fator de Ativação de Plaquetas/farmacologia , Adesividade Plaquetária/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Animais , Arginina/análogos & derivados , Arginina/farmacologia , Aspirina/farmacologia , Endotélio/citologia , Endotélio/efeitos dos fármacos , Epoprostenol/análogos & derivados , Epoprostenol/farmacologia , Feminino , Humanos , Técnicas In Vitro , Neutrófilos/efeitos dos fármacos , Nitroarginina , Fator de Ativação de Plaquetas/antagonistas & inibidores , Adesividade Plaquetária/efeitos dos fármacos , Inibidores da Agregação Plaquetária/farmacologia , Gravidez , CoelhosRESUMO
1. Platelet-activating factor (PAF, 10 nM) did not induce platelet adhesion to endothelial cells cultured in monolayer but it induced their adhesion to protein-coated plastic. However, PAF induced a marked platelet adhesion to endothelial cells when polymorphonuclear leukocytes (PMNs) were present. Lyso-PAF had no effect. 2. Phase-contrast microscopic examination showed that single platelets rather than their aggregates adhered to the endothelial cell surface around aggregating and adhering PMNs. 3. Significant platelet adhesion was induced by PAF at concentrations higher that 0.01 nM with the maximal response at 10 nM. Platelet adhesion occurred within minutes after PAF addition, reaching a maximum approximately after 30 min. Platelet adhesion also occurred significantly at a PMN:platelet ratio of 1:800, and linearly up to 1:50. 4. The PAF-induced platelet adhesion was suppressed by three structurally unrelated PAF antagonists, WEB 2086, ONO 6240 and BN 52021, in a concentration-dependent manner. 5. PAF also increased PMN adhesion to endothelial cell monolayers, which was further augmented by the presence of platelets. 6. The present study demonstrates that PAF induces platelet adhesion to endothelial cells in vitro when PMNs are present and that there is a close interaction between platelets and PMNs in their adhesion to endothelial cells. The present study further suggests that PMNs could play a central role in platelet adhesion to vascular endothlium in certain pathological conditions.
Assuntos
Plaquetas/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Diterpenos , Endotélio Vascular/citologia , Leucócitos/efeitos dos fármacos , Fator de Ativação de Plaquetas/farmacologia , Adesividade Plaquetária/efeitos dos fármacos , Tiazóis , Adenina/metabolismo , Animais , Azepinas/farmacologia , Endotélio Vascular/efeitos dos fármacos , Feminino , Ginkgolídeos , Humanos , Técnicas In Vitro , Lactonas/farmacologia , Neutrófilos/efeitos dos fármacos , Fator de Ativação de Plaquetas/análogos & derivados , Fator de Ativação de Plaquetas/antagonistas & inibidores , Gravidez , Coelhos , Triazóis/farmacologia , Veias Umbilicais/citologiaRESUMO
1. Incubation of endothelial cells with platelets in the absence or the presence of PAF (10 nM) markedly increased platelet cyclic AMP levels, which were significantly decreased by indomethacin (3 microM). Co-incubation of endothelial cells and platelets with polymorphonuclear leukocytes (PMNs) did not change the platelet cyclic AMP levels. 2. Incubation of endothelial cells with platelets in the absence of PAF increased platelet cyclic GMP levels, which were increased 3.5 fold by PAF. These cyclic GMP levels were significantly decreased by NG-nitro-L-arginine (100 microM), and completely by methylene blue (10 microM). When endothelial cells and platelets were co-incubated with PMNs, the cyclic GMP level in the cell mixture was 42.5 and 65.3% lower than that in endothelial cells and platelets without and with PAF stimulation, respectively. 3. PAF induced platelet adhesion to endothelial cells only when PMNs were present. Methylene blue dose-dependently potentiated the PMN-dependent platelet adhesion induced by PAF, although it had no effect in the absence of PMNs. 4. Sodium nitroprusside and 8-bromo cyclic GMP but not dibutyryl cyclic AMP significantly, although partially, inhibited the platelet adhesion. Inhibition of cyclic GMP-specific phosphodiesterase by zaprinast slightly inhibited the PMN-induced platelet adhesion and potentiated the inhibitory effect of 8-bromo cyclic GMP, while these drugs markedly inhibited the adhesion of platelet aggregates induced by PMN sonicates. 5. These results suggest that the impairment by activated PMNs of EDRF-induced platelet cyclic GMP formation is involved in part in the mechanism of PMN-dependent platelet adhesion to endothelial cells induced by PAF in vitro. The precise mechanism still remains to be clarified.
Assuntos
Plaquetas/fisiologia , AMP Cíclico/sangue , GMP Cíclico/sangue , Endotélio Vascular/fisiologia , Leucócitos/fisiologia , Fator de Ativação de Plaquetas/farmacologia , Adesividade Plaquetária/fisiologia , Animais , Plaquetas/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/farmacologia , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Humanos , Técnicas In Vitro , Indometacina/farmacologia , Azul de Metileno/farmacologia , Neutrófilos/fisiologia , Óxido Nítrico/farmacologia , Óxido Nítrico/fisiologia , Nitroprussiato/farmacologia , Inibidores de Fosfodiesterase/farmacologia , Adesividade Plaquetária/efeitos dos fármacos , Purinonas/farmacologia , CoelhosRESUMO
1. Intact platelets and confluent human umbilical vein endothelial cells bound [3H]-Paf-acether (platelet activating factor, [3H]-Paf) at 20 degrees C in the presence of 0.25% (w/v) bovine serum albumin (BSA). 2. [3H]-Paf binding to platelets was inhibited in a concentration-dependent manner by WEB 2086. An excess of WEB 2086 indicated the presence of specific, saturable Paf binding which reached a maximum of 28.3 +/- 3.7 fmol [3H]-Paf per 5 x 10(7) platelets. In platelets, different hetrazepines (WEB 2098, 2105, but not 2118) also inhibited [3H]-Paf binding in a concentration-dependent manner. 3. WEB 2086 partially displaced platelet-bound [3H]-Paf in a concentration-dependent manner reaching a plateau at 400 nM WEB 2086. No further displacement was observed when WEB 2086 and an excess of unlabelled Paf were added together. 4. The hetrazepines inhibited platelet aggregation. Platelet aggregation IC50 values correlated well with the IC50 values of the hetrazepines against [3H]-Paf binding (r2 = 0.99). WEB 2086 shifted the Paf dose-response curve rightwards in a parallel manner. Tested against platelet aggregation the pA2 obtained for WEB 2086 was 7.9. 5. WEB 2086 inhibited [3H]-Paf binding to endothelial cells in a concentration-dependent manner. WEB 2086 also inhibited the Paf-mediated cytosolic calcium increase in endothelial cells with an IC50 value of 23.1 +/- 10.4 nM as compared with an IC50 of 21.6 +/- 10.4 nM WEB 2086 for platelet aggregation. 6. These results demonstrate an inhibition of [3H]-Paf binding to platelets and endothelial cells by different hetrazepines, most probably at the Paf receptor level.
Assuntos
Azepinas/farmacologia , Plaquetas/efeitos dos fármacos , Endotélio Vascular/citologia , Fator de Ativação de Plaquetas/antagonistas & inibidores , Glicoproteínas da Membrana de Plaquetas , Receptores Acoplados a Proteínas G , Triazinas/farmacologia , Triazóis , Cálcio/metabolismo , Citosol/metabolismo , Endotélio Vascular/efeitos dos fármacos , Humanos , Técnicas In Vitro , Agregação Plaquetária/efeitos dos fármacos , Inibidores da Agregação Plaquetária/farmacologia , Receptores de Superfície Celular/metabolismo , Veias Umbilicais/citologiaRESUMO
The effects of phenolic anti-inflammatory drug, MK-447, on prostaglandin (PG) I2 and thromboxane (TX) A2 biosynthesis by rat dental pulp tissue were evaluated in the presence of 10 mM mannitol (MA) or 1 mM ascorbic acid with 0.3 mM Fe2+ (A + F). Although MK-447 alone at 1 and 10 microM had no significant effects, MK-447 at 100 microM stimulated both PGI2 and TXA2 biosynthesis, and suppressed the lipid peroxidation in the pulp tissue as estimated by thiobarbituric acid method. MA also reduced the lipid peroxidation, but had no effect on PG and TX production. However, in the presence of MA, the stimulatory effect of MK-447 was potentiated, and the significant effects were observed at concentrations higher than 1 microM. In contrast, A + F remarkably stimulated the lipid peroxidation, and inhibited both PG and TX biosynthesis. In the presence of A + F, MK-447 showed no stimulatory effect, and contrary, at 100 microM inhibited PG and TX production. These results suggest that the cellular levels of lipid peroxidation exert a significant influence on the effects of phenolic anti-inflammatory drugs like MK-447 on PG biosynthesis. The possible mechanism of action for such drugs has been discussed in view of the significance of lipid peroxidation in inflammatory condition.
Assuntos
Anti-Inflamatórios/farmacologia , Hidroxitolueno Butilado/análogos & derivados , Peróxidos Lipídicos/metabolismo , Prostaglandinas/biossíntese , Animais , Ácido Ascórbico/farmacologia , Hidroxitolueno Butilado/farmacologia , Polpa Dentária/metabolismo , Compostos Ferrosos/farmacologia , Técnicas In Vitro , Masculino , Manitol/farmacologia , Ratos , Ratos Endogâmicos , Tromboxano A2/biossínteseRESUMO
An interaction of the platelet-activating factor (Paf) with endothelial cells was investigated using human umbilical vein endothelial cells. Confluent endothelial cells bound [3H]Paf in the presence of 0.25% fatty acid-free serum albumin after culture in media containing either heat-inactivated foetal calf serum or serum substitute. The Scatchard analysis of the saturated specific [3H]Paf binding showed a Bmax of 2.5 fmol indicating 2800 binding sites per endothelial cell. [3H]Paf binding was partially reversible at 20 degrees and 4 degrees and endothelial cells partially metabolized [3H]Paf at 20 degrees but not at 4 degrees. [3H]Paf binding and Paf-mediated increase of cytosolic free calcium were inhibited by specific Paf receptor antagonists which do not interfere with Paf metabolism. Immortalized umbilical vein endothelial cells bound [3H]Paf specifically after culture in the presence of insulin (20 hr, 0.4 U/mL) with non-specific binding in the absence of insulin. The results show that specific Paf binding mediated calcium flux in human endothelial cells.
Assuntos
Cálcio/metabolismo , Endotélio Vascular/metabolismo , Fator de Ativação de Plaquetas/metabolismo , Transporte Biológico , Células Cultivadas , Citosol/metabolismo , Endotélio Vascular/citologia , Epoprostenol/metabolismo , Humanos , Ligação Proteica , Trítio , Veias UmbilicaisRESUMO
MC3T3-E1 cells actively synthesized and released prostaglandin E2 (PGE2) during culture. PGE2 release was minimal on day 9 and gradually increased with culture up to day 27. DNA content gradually increased until day 27. Alkaline phosphatase (ALP) activity increased up to day 15 and decreased thereafter. In contrast to the decrease in ALP activity, calcium accumulation increased rapidly after day 21, possibly due to mineralization by the cells. Indomethacin, a cyclooxygenase inhibitor, blocked PGE2 production completely at concentrations higher than 0.3 mumol/L. In the presence of indomethacin (3 mumol/L), DNA content was slightly decreased on day 27. Furthermore, ALP activity on day 15 was greater than that of the control and this high activity was maintained until day 27. However, calcium accumulation was not affected by the addition of indomethacin. These results suggest that endogenous PGE2 down-regulates ALP activity and slightly stimulates the proliferation of MC3T3-E1 cells as an autocrine mediator, although it does not directly influence the cells' mineralizing activity.
Assuntos
Dinoprostona/fisiologia , Osteoblastos/fisiologia , Fosfatase Alcalina/metabolismo , Animais , Cálcio/metabolismo , Divisão Celular , Linhagem Celular , Células Clonais , DNA/metabolismo , Indometacina/farmacologia , Cinética , Camundongos , Osteoblastos/efeitos dos fármacosRESUMO
The effects of 3 bisphosphonates, AHBuBP, AHPrBP, and Cl2MBP on cell growth, alkaline phosphatase (ALP) activity, mineralization, and prostaglandin E2 (PGE2) synthesis in the clonal osteoblast-like cell line MC3T3-E1 were studied. These bisphosphonates had essentially similar effects on growth and the osteoblastic functions of the cells, i.e., they had no inhibitory effects on cell growth except at higher concentrations, they increased ALP activity, and inhibited PGE2 production. In the presence of AHBuBP, ALP activity was higher than that in the control after day 6 of culture. Lower concentrations of AHBuBP slightly facilitated mineralization by the cells. It is probable that bisphosphonates enhance the functions of osteoblasts in certain concentration and that the inhibition of endogenous PGE2 production may be involved in the mechanism of action of bisphosphonates.
Assuntos
Fosfatase Alcalina/metabolismo , Calcificação Fisiológica/efeitos dos fármacos , Dinoprostona/biossíntese , Difosfonatos/farmacologia , Osteoblastos/efeitos dos fármacos , Alendronato/farmacologia , Animais , Cálcio/metabolismo , Linhagem Celular , Ácido Clodrônico/farmacologia , Meios de Cultura , DNA/análise , Glicerofosfatos/metabolismo , Camundongos , Osteoblastos/metabolismo , PamidronatoRESUMO
Platelet-activating factor (PAF-acether), but not lyso PAF, stimulated the production of both PGI2 and TXA2 by rat dental pulp tissue in vitro. However, there were differences in the dose- and time-dependence of the stimulatory effects. PAF-acether antagonists, Bn 52021, CV 3988 and kadsurenone, dose dependently inhibited PAF-acether-induced PG production. BN 52021, CV 3988 also dose dependently inhibited TX production, but kadsurenone was almost without effect on TX production. Pretreatment of the tissues with PAF-acether or phorbol 12-myristate 13-acetate completely abolished the effect of the second challenge with PAF-acether. The stimulatory effects of PAF-acether and the calcium ionophore A23187 on PGI2 production were completely blocked by removal of extracellular calcium, whereas the effects on TXA2 production were not. TMB-8, an intracellular calcium antagonist, completely inhibited PAF-acether-induced PG production, whereas it slightly inhibited TX production. H-7, a protein kinase C inhibitor, and neomycin, a phospholipase C inhibitor, completely inhibited PAF-acether-induced PG and TX production, whereas W-7, a calmodulin inhibitor, did not. These results suggest that PAF-acether stimulates PGI2 and TXA2 production in rat dental pulp by interacting with distinct PAF-acether receptors, and that these receptors are coupled to independent signal transduction pathways which have a different dependence on extra- and intracellular calcium.
Assuntos
Polpa Dentária/metabolismo , Epoprostenol/biossíntese , Fator de Ativação de Plaquetas/farmacologia , Tromboxano A2/biossíntese , Animais , Calcimicina/farmacologia , Cálcio/farmacologia , Polpa Dentária/efeitos dos fármacos , Técnicas In Vitro , Cinética , Masculino , Fator de Ativação de Plaquetas/antagonistas & inibidores , Ratos , Ratos Endogâmicos , Estimulação QuímicaRESUMO
The effect of 5-hydroxytryptamine (5-HT) on the release of prostacyclin and thromboxane (TX) A2 from isolated rat dental pulp was evaluated. 5-HT (1-1,000 microM) caused a dose-dependent and marked stimulation of the release of prostacyclin but not TXA2. Of the 5-HT-related indolealkylamines tested, only tryptamine had a similar stimulatory effect while tryptophan and 5-hydroxytryptophan had no effect. Neither histamine (100 microM) nor bradykinin (100 microM) had such an effect. Our results suggest the possible involvement of 5-HT receptors in 5-HT-induced stimulation of prostacyclin production in rat dental pulp.
Assuntos
Polpa Dentária/metabolismo , Epoprostenol/metabolismo , Serotonina/farmacologia , Tromboxano A2/metabolismo , Animais , Polpa Dentária/efeitos dos fármacos , Técnicas In Vitro , Masculino , Ratos , Ratos Endogâmicos , Estimulação QuímicaRESUMO
5-Hydroxytryptamine (5-HT) stimulated prostacyclin release, measured by radioimmunoassay as 6-keto-prostaglandin F1 alpha, from rat aorta in a dose-dependent manner (3 X 10(-7)-10(-4) M). A statistically significant stimulation was observed at concentrations higher than 3 X 10(-6) M. Norepinephrine also increased prostacyclin release but only at a high concentration (10(-4) M) and histamine (3 X 10(-5) and 10(-4) M) had no significant effect. Among some structurally 5-HT-related analogues, only tryptamine exhibited a dose-dependent stimulatory effect on prostacyclin release but it was slightly less potent than 5-HT. Tryptophan and 5-hydroxytryptophan had no effects whereas 5-hydroxyindoleacetic acid at 10(-4) M stimulated slightly. Prostacyclin release stimulated by 5-HT was depressed by non-specific 5-HT antagonists, methysergide, mianserin and cyproheptadine. In contrast, a specific 5-HT2 antagonist ketanserin (3 X 10(-7)-10(-5) M) had no antagonistic effect. These results suggest that 5-HT stimulates the release of prostacyclin from rat aorta by interaction with receptors distinct from its 5-HT2 subtype.
Assuntos
Epoprostenol/metabolismo , Receptores de Serotonina/fisiologia , Serotonina/farmacologia , Animais , Aorta Torácica/metabolismo , Técnicas In Vitro , Masculino , Norepinefrina/farmacologia , Prostaglandina-Endoperóxido Sintases/metabolismo , Ratos , Ratos Endogâmicos , Receptores de Serotonina/efeitos dos fármacos , Receptores de Serotonina/metabolismo , Serotonina/análogos & derivados , Tromboxano A2/farmacologiaRESUMO
The effect of docosahexaenoic acid treatment on intracellular Ca(2+) dynamics in rat vascular smooth muscle cells stimulated with 5-hydroxytryptamine (5-HT) has been investigated in order to elucidate one of the mechanisms for its beneficial effect on cardiovascular disorders. The treatment of cells with 30 microM docosahexaenoic acid for 2 days inhibited an increase in intracellular Ca(2+) concentration induced by 5-HT (10 microM) and a depolarizing concentration of KCl (80 mM). Docosahexaenoic acid treatment significantly inhibited divalent cation influx stimulated by 5-HT and KCl, as measured by Mn(2+) quenching method, whereas had no effect on 5-HT-induced Ca(2+) release from the internal stores. Docosahexaenoic acid treatment also significantly inhibited 5-HT receptor-mediated Ca(2+) influx through Ni(2+)-insensitive channels that were distinct from store-operated channels. These results suggest that the specific inhibition of intracellular Ca(2+) dynamics in vascular smooth muscle cells may contribute to the beneficial properties of docosahexaenoic acid on cardiovascular disorders.
Assuntos
Cálcio/metabolismo , Ácidos Docosa-Hexaenoicos/farmacologia , Músculo Liso Vascular/efeitos dos fármacos , Receptores de Serotonina/fisiologia , Serotonina/farmacologia , Animais , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Células Cultivadas , Relação Dose-Resposta a Droga , Masculino , Manganês/metabolismo , Músculo Liso Vascular/citologia , Músculo Liso Vascular/metabolismo , Níquel/farmacologia , Cloreto de Potássio/farmacologia , Ratos , Ratos WistarRESUMO
The effect of noradrenaline on 5-hydroxytryptamine (5-HT) release from isolated mouse ileal tissues was investigated. Noradrenaline, but not isoprenaline, at 1 microM stimulated 5-HT release, an effect which was inhibited by yohimbine, an alpha(2)-adrenoceptor antagonist, but not by bunazosin, an alpha(1)-adrenoceptor antagonist. alpha(2)-Adrenoceptor agonists, UK 14,304 (5-bromo-6-(2-imidazolin-2-yl-amino)-quinoxaline) and clonidine at a higher concentration (10 microM) also stimulated 5-HT release, while alpha(1)-adrenoceptor agonists, methoxamine and phenylephrine, had no effect. The effect of noradrenaline was completely abolished in ileal tissues isolated from mouse treated with pertussis toxin (100 microg/kg, i.v.) for 2 days. These results suggest that noradrenaline causes 5-HT release from enterochromaffin cells in mouse ileal tissues via alpha(2)-adrenoceptor subtypes coupled to a pertussis toxin-sensitive G protein.
Assuntos
Íleo/efeitos dos fármacos , Norepinefrina/farmacologia , Receptores Adrenérgicos alfa 2/fisiologia , Serotonina/metabolismo , Agonistas de Receptores Adrenérgicos alfa 2 , Antagonistas de Receptores Adrenérgicos alfa 2 , Agonistas alfa-Adrenérgicos/farmacologia , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Tartarato de Brimonidina , Clonidina/farmacologia , Íleo/metabolismo , Técnicas In Vitro , Masculino , Metoxamina/farmacologia , Camundongos , Camundongos Endogâmicos ICR , Toxina Pertussis , Fenilefrina/farmacologia , Quinazolinas/farmacologia , Quinoxalinas/farmacologia , Fatores de Virulência de Bordetella/farmacologia , Ioimbina/farmacologiaRESUMO
The effects of Ni2+, a non-selective cation channel inhibitor, on 5-hydroxytryptamine (5-HT)- and angiotensin II (Ang II)-induced intracellular Ca2+ dynamics in rat aortic smooth muscle cells were investigated. Ni2+ (1 mM) significantly inhibited the transient increase in intracellular Ca2+ concentration ([Ca2+]i) induced by Ang II (100 nM) in aortic smooth muscle cells, as measured using fura-2. However, Ni2+ did not suppress the transient increase in Ca2+ influx induced by 5-HT (10 microM), while significantly suppressed the sustained increase. Ca2+ influx evoked by high KCl (80 mM), thapsigargin (TG) (1 microM) or depletion of intracellular Ca2+ store was almost completely suppressed by Ni2+. Ni2+ had no effect on 5-HT-induced inositol triphosphate production and Ca2+ release from the intracellular store(s). These results suggest that 5-HT, but not Ang II, induces transient Ca2+ influx through Ni2+-insensitive Ca2+ channels, which are distinguishable from the voltage-dependent or store-operated Ca2+ channels.
Assuntos
Canais de Cálcio/efeitos dos fármacos , Cálcio/metabolismo , Músculo Liso Vascular/efeitos dos fármacos , Níquel/farmacologia , Serotonina/farmacologia , Angiotensina II/farmacologia , Animais , Cálcio/farmacologia , Relação Dose-Resposta a Droga , Masculino , Músculo Liso Vascular/citologia , Músculo Liso Vascular/metabolismo , Cloreto de Potássio/farmacologia , Ratos , Ratos Wistar , Tapsigargina/farmacologiaRESUMO
The antiemetic activity of N-3389 (endo-3,9-dimethyl-3,9-diazabicyclo[3,3,1]non-7-yl-1 H-indazole-3-carboxamide dihydrochloride), a new 5-HT3 and 5-HT4 receptor antagonist, against cisplatin-, cyclophosphamide- and copper sulfate-induced emesis was investigated using ferrets. We also examined the effects of these agents on abdominal afferent vagus nerve activity in anesthetized ferrets. Both intraperitoneal (0.1-1.0 mg/kg) and oral (0.1-1.0 mg/kg) administration of N-3389 produced dose-dependent antiemetic effects. The time-course of cisplatin (10 mg/kg, i.p.)-induced emesis in another group of ferrets paralleled the increase in abdominal afferent vagus nerve activity induced by cisplatin (10 mg/kg, i.p.) and was inhibited by pretreatment with N-3389 (1.0 mg/kg, i.v.). Furthermore, the cisplatin (10 mg/kg, i.p.)-induced increase in abdominal afferent vagus nerve activity was markedly reduced by an additional injection of N-3389 (0.1-1.0 mg/kg, i.v.) in a dose-dependent manner. The antiemetic effects exhibited by N-3389 are probably due to the inhibition of 5-HT3 and 5-HT4 receptors on the abdominal afferent vagus nerves.