RESUMO
We have previously shown a significant decrease in the ethylene production in tomato fruit from the RIN/rin genotype. In this present study, we evaluated the amount of 1-aminocyclopropane-1-carboxylic acid (ACC) and the gene expression and enzymatic activities of ACC synthase (ACS) and ACC oxidase (ACO) to find which type of regulation influenced this low ethylene production. The results suggest that the decreased ethylene production was due to transcriptional regulation of the ACS and ACO genes by the heterozygous effect of the rin gene.
Assuntos
Etilenos/biossíntese , Frutas/metabolismo , Solanum lycopersicum/metabolismo , Aminoácido Oxirredutases/genética , Aminoácido Oxirredutases/metabolismo , Aminoácidos Cíclicos/metabolismo , Quimera , Frutas/genética , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Liases/genética , Liases/metabolismo , Solanum lycopersicum/genética , MutaçãoRESUMO
The ripening inhibitor (rin) mutant tomato yields non-ripening fruit, and the rin hybrid fruit (RIN/rin) shows an intermediate phenotype between the wild and mutant fruit, that is, red-ripe and extended shelf life. We found by a microarray analysis that the genes encoding possible allergenic proteins were expressed at a significantly lower level in the rin hybrid fruit than in the wild-type fruit. These allergenic proteins, which were beta-fructofuranosidase and polygalacturonase 2A (PG-2A), were confirmed to accumulate at a lower level in the rin hybrid fruit than in the wild-type fruit. The immunoglobulin E (IgE) in serum from a tomato-allergic patient showed lower reactivity to the extract of the rin hybrid fruit than to that of the wild fruit. These results suggest that the rin gene has the potential to regulate allergen accumulation in tomato fruit.
Assuntos
Antígenos de Plantas/genética , Antígenos de Plantas/imunologia , Hipersensibilidade Alimentar/imunologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Solanum lycopersicum/efeitos adversos , Quimera/genética , Expressão Gênica , Genes de Plantas , Humanos , Imunoglobulina E/sangue , Solanum lycopersicum/genética , Solanum lycopersicum/imunologia , Mutação , Análise de Sequência com Séries de Oligonucleotídeos , Poligalacturonase/genética , Poligalacturonase/imunologia , beta-Frutofuranosidase/genética , beta-Frutofuranosidase/imunologiaRESUMO
Oxygenated lycopenes, (2S*,5S*,6R*)-2,6-cyclolycopene-1-methoxy-5-ol, (2S*,5S*,6R*)-1,16-didehydro-2,6-cyclolycopene-5-ol and (3R,6'R)-3-hydroxy-3',4'-didehydro-beta,gamma-carotene (anhydrolutein I) were isolated from tomato puree. The structures of these compounds were elucidated on the basis of spectroscopic evidence.