Induction of male-like mandibles in XX individuals of a stag beetle by gene knockdown of a feminizer gene transformer.

Males and females share most of the genome, but many animals show different phenotypes between the sexes, known as sexual dimorphism. Many insect species show extreme sexual dimorphism, including beetles with "weapon traits" represented by extremely developed horns and mandibles. Existing studies of sex-specific development of beetle weapon traits suggest that sex-specific gene expression plays an important role. On the other hand, contributions of the Y-chromosome, which may potentially carry genes necessary for male development, to weapon trait expression have not been examined. In holometabolous insects, including beetles, the feminizing gene transformer (tra) is roughly conserved in its feminizing function. Only females express a functional isoform of Tra, which causes female differentiation. Knocking down tra in females leads to male tissue differentiation, enabling us to analyze male phenotypes in individuals lacking a Y-chromosome (XX-males). In this study, we investigate whether the Y-chromosome is necessary for stag beetles to express male-specific weapon traits by comparing tra-knockdown-induced XX-males with natural XY males. We show that XX-males could express weapons (enlarged mandibles) as in XY-males. These results suggest that the Y-chromosome does not have a major role in weapon trait expression in this species.

Body size and digestive system shape resource selection by ungulates: A cross-taxa test of the forage maturation hypothesis.

The forage maturation hypothesis (FMH) states that energy intake for ungulates is maximised when forage biomass is at intermediate levels. Nevertheless, metabolic allometry and different digestive systems suggest that resource selection should vary across ungulate species. By combining GPS relocations with remotely sensed data on forage characteristics and surface water, we quantified the effect of body size and digestive system in determining movements of 30 populations of hindgut fermenters (equids) and ruminants across biomes. Selection for intermediate forage biomass was negatively related to body size, regardless of digestive system. Selection for proximity to surface water was stronger for equids relative to ruminants, regardless of body size. To be more generalisable, we suggest that the FMH explicitly incorporate contingencies in body size and digestive system, with small-bodied ruminants selecting more strongly for potential energy intake, and hindgut fermenters selecting more strongly for surface water.

[Acquired hypofibrinogenemia in patients with leukemia during steroid therapy].

Acquired hypofibrinogenemia is observed in patients with severe liver disease, disseminated intravascular coagulation, and high-volume perioperative fluid replacement. In lymphoblastic leukemia, hypofibrinogenemia is most frequently caused by the administration of L-asparaginase. Here we report the cases of two patients with acquired hypofibrinogenemia that occurred during steroid-containing chemotherapy treatment against lymphoblastic blast crisis of chronic myeloid leukemia in the first case and acute lymphoblastic leukemia in the second case. Administration of steroids repeatedly and promptly caused hypofibrinogenemia, irrespective of the products (prednisolone, dexamethasone, or methylprednisolone) or routes (oral or intravenous) that were used. Monitoring of the fibrinogen levels, especially during the first course of steroid therapy, would be useful for early diagnosis.

A Single Pheromone Receptor Gene Conserved across 400 My of Vertebrate Evolution.

Pheromones are crucial for eliciting social and sexual behaviors in diverse animal species. The vomeronasal receptor type-1 (V1R) genes, encoding members of a pheromone receptor family, are highly variable in number and repertoire among mammals due to extensive gene gain and loss. Here, we report a novel pheromone receptor gene belonging to the V1R family, named ancient V1R (ancV1R), which is shared among most Osteichthyes (bony vertebrates) from the basal lineage of ray-finned fishes to mammals. Phylogenetic and syntenic analyses of ancV1R using 115 vertebrate genomes revealed that it represents an orthologous gene conserved for >400 My of vertebrate evolution. Interestingly, the loss of ancV1R in some tetrapods is coincident with the degeneration of the vomeronasal organ in higher primates, cetaceans, and some reptiles including birds and crocodilians. In addition, ancV1R is expressed in most mature vomeronasal sensory neurons in contrast with canonical V1Rs, which are sparsely expressed in a manner that is consistent with the "one neuron-one receptor" rule. Our results imply that a previously undescribed V1R gene inherited from an ancient Silurian ancestor may have played an important functional role in the evolution of vertebrate vomeronasal organ.

Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma.

Materials and Methods: Using flow cytometry, we identified and quantified Group 2 innate lymphocytes, T helper 2 cells, follicular helper T cells, and T helper 17 cells in peripheral blood samples from 49 individuals with asthma. We then conducted cross-sectional analyses to assess relationships between levels of these immune cells and lung function parameters, including the percentage predicted forced expiratory volume in 1 s (%FEV1). We also examined correlations between the proportions of immune cells and type 2 biomarkers. Results: Proportions of CXCR5+ follicular helper T cells in human peripheral blood, as opposed to Group 2 innate lymphoid cells (ILC2) or T helper 2 cells, were significantly higher in cases with %FEV1 < 80% compared to those with %FEV1 ≥ 80%. Further, these proportions correlated negatively with %FEV1 and positively with blood eosinophil counts. Conclusions: The proportion of circulating follicular helper T cells, but not T helper 2 cells or Group 2 innate lymphoid cells, may reflect the presence of airway obstruction caused by persistent type 2 inflammation.

[Psychological processes predicting the English communication behavior of Japanese high school students].

This study presents a new model of psychological processes to predict English communication behaviors of Japanese high school students. Various models have been proposed in Japan, based mainly on Canadian models, to predict second-language communication behaviors. This study shows problems with the previous models in Japan and introduces a new model from the perspective of "Expectancy-Value Theory". Questionnaire Survey 1 compared the previous model and the modified models which suggested that a new psychological variable, "Value in English Communication", was necessary to construct the new model. In Survey 2, the new model was further modified by incorporating into it various English learning values which the Japanese have. This study makes a significant contribution to studies and practices of teaching English as a foreign language in Japan.

Does macro-level general trust influence second language communication?: targeting prefectural capitals.

OBJECTIVES: This study investigated the macro-level effects of general trust on second language willingness to communicate (WTC) among Japanese individuals residing in 20 prefectural capitals. Previous research has shown the impact of individual-level general trust on WTC; however, macro-level effects have not been explored. RESULTS: Multilevel analysis in this research revealed that individual- and prefectural capital-level general trust had a positive influence on WTC in English for Japanese people. Moreover, macro-level effects had a strong predictive power for WTC, thus, highlighting the potential of general trust to facilitate positive connections between people from different cultures.

Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest.

Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.

Abiotic and biotic factors controlling the dynamics of soil respiration in a coastal dune ecosystem in western Japan.

In this study, we examined the abiotic and biotic factors controlling the dynamics of soil respiration (Rs) while considering the zonal distribution of plant species in a coastal dune ecosystem in western Japan, based on periodic Rs data and continuous environmental data. We set four measurement plots with different vegetation compositions: plot 1 on bare sand; plot 2 on a cluster of young Vitex rotundifolia seedlings; plot 3 on a mixture of Artemisia capillaris and V. rotundifolia; and plot 4 on the inland boundary between the coastal vegetation zone and a Pinus thunbergii forest. Rs increased exponentially along with the seasonal rise in soil temperature, but summer drought stress markedly decreased Rs in plots 3 and 4. There was a significant positive correlation between the natural logarithm of belowground plant biomass and Rs in autumn. Our findings indicate that the seasonal dynamics of Rs in this coastal dune ecosystem are controlled by abiotic factors (soil temperature and soil moisture), but the response of Rs to drought stress in summer varied among plots that differed in dominant vegetation species. Our findings also indicated that the spatial dynamics of Rs are mainly controlled by the distribution of belowground plant biomass and autotrophic respiration.

Nutrient-regulated antisense and intragenic RNAs modulate a signal transduction pathway in yeast.

The budding yeast Saccharomyces cerevisiae alters its gene expression profile in response to a change in nutrient availability. The PHO system is a well-studied case in the transcriptional regulation responding to nutritional changes in which a set of genes (PHO genes) is expressed to activate inorganic phosphate (Pi) metabolism for adaptation to Pi starvation. Pi starvation triggers an inhibition of Pho85 kinase, leading to migration of unphosphorylated Pho4 transcriptional activator into the nucleus and enabling expression of PHO genes. When Pi is sufficient, the Pho85 kinase phosphorylates Pho4, thereby excluding it from the nucleus and resulting in repression (i.e., lack of transcription) of PHO genes. The Pho85 kinase has a role in various cellular functions other than regulation of the PHO system in that Pho85 monitors whether environmental conditions are adequate for cell growth and represses inadequate (untimely) responses in these cellular processes. In contrast, Pho4 appears to activate some genes involved in stress response and is required for G1 arrest caused by DNA damage. These facts suggest the antagonistic function of these two players on a more general scale when yeast cells must cope with stress conditions. To explore general involvement of Pho4 in stress response, we tried to identify Pho4-dependent genes by a genome-wide mapping of Pho4 and Rpo21 binding (Rpo21 being the largest subunit of RNA polymerase II) using a yeast tiling array. In the course of this study, we found Pi- and Pho4-regulated intragenic and antisense RNAs that could modulate the Pi signal transduction pathway. Low-Pi signal is transmitted via certain inositol polyphosphate (IP) species (IP7) that are synthesized by Vip1 IP6 kinase. We have shown that Pho4 activates the transcription of antisense and intragenic RNAs in the KCS1 locus to down-regulate the Kcs1 activity, another IP6 kinase, by producing truncated Kcs1 protein via hybrid formation with the KCS1 mRNA and translation of the intragenic RNA, thereby enabling Vip1 to utilize more IP6 to synthesize IP7 functioning in low-Pi signaling. Because Kcs1 also can phosphorylate these IP7 species to synthesize IP8, reduction in Kcs1 activity can ensure accumulation of the IP7 species, leading to further stimulation of low-Pi signaling (i.e., forming a positive feedback loop). We also report that genes apparently not involved in the PHO system are regulated by Pho4 either dependent upon or independent of the Pi conditions, and many of the latter genes are involved in stress response. In S. cerevisiae, a large-scale cDNA analysis and mapping of RNA polymerase II binding using a high-resolution tiling array have identified a large number of antisense RNA species whose functions are yet to be clarified. Here we have shown that nutrient-regulated antisense and intragenic RNAs as well as direct regulation of structural gene transcription function in the response to nutrient availability. Our findings also imply that Pho4 is present in the nucleus even under high-Pi conditions to activate or repress transcription, which challenges our current understanding of Pho4 regulation.

Health effects of dust storms: subjective eye and respiratory system symptoms in inhabitants in Mongolia.

The cross-sectional study described here aimed to investigate potential health effects associated with dust storms in Mongolia. The authors surveyed the subjective symptoms of the eyes (e.g., bloodshot eyes) and respiratory system (e.g., coughing) among inhabitants in different living environments (urban and desert) immediately after a dust storm. The subjects studied lived either in an urban area (n = 36 residents), or a desert area (n = 87 nomads). Information concerning eye and respiratory system symptoms was obtained by face-to-face household interviews. Multiple logistic regression analysis was performed on the relationships between the subjective symptoms and the different living environments adjusting for age, gender, and smoking status. The occurrence of lacrimation, an eye symptom, was higher among the desert area participants compared to those residing in the urban area. Results suggest that the occurrence of eye lacrimation is related to dust storms.

The influence of psychological network on the willingness to communicate in a second language.

This study investigated the effect of the psychological network on the willingness to communicate in English among Japanese people. Previous studies have shown that psychological factors affect the willingness to communicate in English for Japanese people. However, the network structure of psychological factors and their effects have not been revealed yet. The present study conducted a network analysis with 644 Japanese people. Consequently, the edge between perceived communication competence and the willingness to communicate in the first or second language was very strong. Node centrality strength showed that these factors were central in the network structure. The results of the network analysis show the effect of psychological networks on the willingness to communicate in a second language, which will be beneficial for language education.

The Influence of Socio-Ecological Networks on Willingness to Communicate in English for Japanese People.

This study investigates the effect of socio-ecological networks on the willingness to communicate (WTC) in English among Japanese people. Previous studies have shown that relational mobility (socio-ecological factor), which is defined as the availability of opportunities to choose new relationship partners, positively affects the WTC in English for Japanese people. However, the network structure of the variables of relational mobility and its effects have not been revealed yet. The present study conducted network analysis with 474 Japanese university students and found the two clusters that correspond to the dimensions of relational mobility in the partial correlation network. Three variables regarding opportunities to meet new people and leave current relationships positively affected the WTC in English; one had a negative effect. Centrality indices, such as nodes strength, betweenness, and closeness, revealed the centrality of several variables in the network. Bootstrapping methods showed the trustworthiness of the estimated network structure and centrality indices as well as edges and variables whose effects differed significantly from that of others. Contrary to the regression analysis results, the network analysis findings can help us understand the in-depth effect of relational mobility on the WTC in a second language, which will prove useful for intervention studies.

The influence of networks of general trust on willingness to communicate in English for Japanese people.

This study investigates the effect of a network of general trust on the willingness to communicate in English among Japanese people. Previous studies have shown that general trust positively affects the willingness to communicate in English for Japanese people. However, the network structure of general trust and its effects have not yet been revealed. The present study conducted a network analysis with 761 Japanese university students and 601 Japanese social survey participants, for 1362 participants total. Four variables regarding general trust positively affected the willingness to communicate in English for all participants, whereas one variable had a negative effect if each network was estimated for only university students or social survey participants. Centrality indices, such as node strength, closeness, and expected influence, revealed the centrality of several variables in the network of all participants. Bootstrapping methods showed the trustworthiness of the estimated edges and centrality indices. Contrary to the regression analysis, the network analysis can help us understand the profound effect of general trust on the willingness to communicate in a second language, which will prove useful for intervention studies.

The half-life of Ascaris lumbricoides prevalence in Japanese school children.

In the present study, we examined the dynamic of school-health-based parasite control and the related socio-economic influences. This is an ecological study based on data from 46 prefectures in Japan. The exponential decay of Ascaris lumbricoides prevalence was calculated by iterative least-squares method. Pearson's correlation and multiple linear regression model analysis were performed to assess the associations between the prevalence of Ascaris lumbricoides in Japanese school children and socio-economic variables such as the prefecture income per capita, the percentage of primary industry, the population density per 1 km2, the diffusion rate of population under water supply, and the percentage of upper secondary school enrollment. The results indicated that the parasite carrier rate was higher in younger students. The half-life of Ascaris lumbricoides prevalence was approximately 3 years with significant variation among prefectures. Multiple regression analyses showed that the decrease of infection in elementary and lower secondary school children had a significant positive association with primary industry and a significant negative association with prefecture income per capita. The school-health-based parasite intervention differs by prefecture and has changed over time according to the respective prefectural stage of economic development.

Health-related quality of life and recognition of desertification among inhabitants of the Loess Plateau region of China: findings for city and village communities.

This article elucidates the health-related quality of life (HRQOL) the recognition of desertification among people living in the semi-arid Loess Plateau of China. HRQOL was assessed with a three-dimensional survey of general health perception, vitality, and general mental health based on a 36-item short-form health survey (SF-36). Scores for general health perception were approximately the same in the city and the village communities. Vitality and mental health scores were significantly lower for women in the village communities than for other groups. In the village communities, HRQOL was significantly and positively correlated with income. The inhabitants of the village communities were more satisfied with their life situation than those in the city, in spite of the economic gap between them. Levels of recognition of desertification were lower in the village communities than in the city.

Movements of dams milked for fermented horse milk production in Mongolia.

Airag, (Fermented horse milk) is a traditional milk product in Mongolia. Herders separate foals from their dams and tie them at a milking site during the daytime to produce airag. To evaluate the effects of horse management on the movement of dams, we tracked three dams in a herd in camp 1 during summer and camp 2 during autumn of 2013 and analyzed their movements during the milking (daytime) and non-milking (nighttime) periods in an area famous for its high-quality airag. Dams were gathered every 1.7 ± 0.0 h between 07.46 and 15.47 hours at the milking sites and milked 4.6 ± 0.2 times/day during the study period (86 days). Daily cumulative and maximum linear distances from the milking sites were longer (P < 0.01) during the non-milking period than during the milking period. Daily home ranges were 91 and 26 times greater during the non-milking period (P < 0.001) in camps 1 and 2, respectively. The greater range during the non-milking period would reflect the spatial distributions of water, salt and forage. The dams initially used similar areas and gradually shifted their daily home ranges after several days. This shift suggests that the dams grazed farther afield as forage availability declined around the milking site. For better airag production and sustainable pasture use, our results provide insights useful for evaluating the effects of milking management on vegetation and soil in those pastures, for selecting the appropriate milking times and frequency, and for choosing the right timing to shift milking sites.

GTOP: a database of protein structures predicted from genome sequences.

Large-scale genome projects generate an unprecedented number of protein sequences, most of them are experimentally uncharacterized. Predicting the 3D structures of sequences provides important clues as to their functions. We constructed the Genomes TO Protein structures and functions (GTOP) database, containing protein fold predictions of a huge number of sequences. Predictions are mainly carried out with the homology search program PSI-BLAST, currently the most popular among high-sensitivity profile search methods. GTOP also includes the results of other analyses, e.g. homology and motif search, detection of transmembrane helices and repetitive sequences. We have completed analyzing the sequences of 41 organisms, with the number of proteins exceeding 120 000 in total. GTOP uses a graphical viewer to present the analytical results of each ORF in one page in a 'color-bar' format. The assigned 3D structures are presented by Chime plug-in or RasMol. The binding sites of ligands are also included, providing functional information. The GTOP server is available at http://spock.genes.nig.ac.jp/~genome/gtop.html.

Obesity and the risk of diabetes mellitus in middle-aged Japanese men.

The morbidity of diabetes mellitus is increasing gradually in Japanese populations. It is important to clarify the risk factors of diabetes in Japanese populations in order to take adequate measures against the increasing morbidity of diabetes. In order to evaluate the link between past and concurrent obesity and diabetes in middle-aged Japanese men, we conducted a worksite-based historical cohort study in Okayama, Japan in 1999. Annual health examination data of middle-aged male workers in a worksite were collected. The relative risks of past and concurrent obesity for developing diabetes were calculated. Subjects with a past history of obesity at between 40 and 50 years of age had a significantly higher risk of developing diabetes by age 55 than did subjects in the normal weight group. These results suggest that, in order to prevent diabetes in middle-aged Japanese men, health guidance for normal weight maintenance should be provided not only for middle-aged men, but also for men under age 40.