1.
Plant Cell Physiol
; 42(8): 873-8, 2001 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11522915
RESUMO
CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.