Fabrication of Chitosan/PEGDA Bionanocomposites for Enhanced Drug Encapsulation and Release Efficiency.

Drug delivery systems (DDS) have evolved in the last decades with the development of hydrogels and particles. However, challenges such as high systemic uptake, side effects, low bioavailability, and encapsulation efficiency continue to be significant hurdles faced by such DDSs. Particles and hydrogels can be specifically designed for targeted DDSs to mitigate some of these problems. This study developed chitosan (Cs) particles (Ps) and composite films using poly(ethylene glycol) diacrylate (PEGDA) as a copolymer to encapsulate gentamicin (GtS) for drug delivery. We demonstrated that lysozyme degrades the chitosan ß-1,4 glycosidic bonds to release GtS. PEGDA increased drug encapsulation efficiency by shielding the repelling forces of like charges between Cs and GtS. The data show that PEGDA does not hinder enzymatic degradation while increasing drug encapsulation efficiency and producing more homogeneous particles. Additionally, we utilized Michael's reaction to cross-link Cs, CsPs, and PEGDA to produce a film designed for drug delivery. The film is an anchor for CsPs to prevent premature drug release. The cross-linking of Cs and PEGDA does not affect lysozyme activity, and CsPs could successfully release GtS without affecting GtS activity.

Covalent Crosslinking of Colloidal Cellulose Nanocrystals for Multifunctional Nanostructured Hydrogels with Tunable Physicochemical Properties.

Cellulose nanocrystals (CNCs) have shown promise for the development of multifunctional materials for many research communities, ranging from bioresource engineering and biomedical engineering to materials science and engineering. However, accessible hydroxyl (OH) groups on the surface of colloidal CNCs at the (11̅0)ß/(100)α and (110)ß/(010)α facets and the intermolecular hydrogen bonding (H-bonds) between these OH groups account for the instability of self-assembled CNC structures in moist environments, limiting their practical uses to dry media. In this work, accessible OH groups of CNCs were crosslinked using two crosslinkers, that is, glutaraldehyde (GA) and epichlorohydrin (EC), to form nanoparticle-based hydrogels with tunable physicochemical properties. The intensity of the intermolecular H-bonds was controlled by the type and concentration of crosslinkers as well as the CNC concentration. Rheological analyses through the loss tangent were used to determine the degree of crosslinking with maximal values beyond 90%. Fourier-transform infrared spectroscopy demonstrated that H-bond intensity was inversely proportional to the degree of crosslinking for both GA and EC, indicating a dissimilar crosslinking mechanism for GA and EC in acidic and alkaline pH conditions, respectively. Atomic force microscopy and wettability analyses showed a significant increase in the surface roughness from 3.2 ± 0.41 nm (pure CNC) to 31.5 ± 1.08 nm (CNCs crosslinked by GA) and 23.8 ± 0.14 nm (CNCs crosslinked by EC) and water contact angle from 13° (pure CNC) to 108° (CNCs crosslinked by GA) and 104° (CNCs crosslinked by EC). Moreover, optimum water absorption values were found at 157.67 ± 2.01 g and 173.59 ± 1.26 g of water for 1 g of freeze-dried hydrogels for 10% GA and 1% EC, respectively. The results aligned with reaction conditions that led to maximal degrees of crosslinking and indicated the transformation of surface chemistry from a hydrophilic to a hydrophobic network as well as tunable topology and aqueous stability of self-assembled structures made from crosslinked CNCs. This technology demonstrated the potential of crosslinked CNCs with tunable physicochemical properties for use as advanced building blocks to produce 2D and 3D structures for their related functions.

Polysaccharide-based porous biopolymers for enhanced bioaccessibility and bioavailability of bioactive food compounds: Challenges, advances, and opportunities.

Bioactive food compounds, such as lycopene, curcumin, phytosterols, and resveratrol, have received great attention due to their potential health benefits. However, these bioactive compounds (BCs) have poor chemical stability during processing and low bioavailability after consumption. Several delivery systems have been proposed for enhancing their stability and bioavailability. Among these methods, porous biopolymers have emerged as alternative encapsulation materials, as they have superior properties like high surface area, porosity, and tunable surface chemistry to entrap BCs. This reduces the crystallinity (especially for the lipophilic ones) and particle size, and in turn, increases solubilization and bioavailability. Also, loading BCs into the porous matrix can protect them against environmental stresses such as light, heat, oxygen, and pH. This review introduces polysaccharide-based porous biopolymers for improving the bioaccessibility/bioavailability of bioactive food compounds and discusses their recent applications in the food industry. First, bioaccessibility and bioavailability are described with a special emphasis on the factors affecting them. Then, porous biopolymer fabrication methods, including supercritical carbon dioxide (SC-CO2 ) drying, freeze-drying, and electrospinning and electrospraying, are thoroughly discussed. Finally, common polysaccharide-based biopolymers (i.e., starch, nanocellulose, alginate, and pectin) used for generating porous materials are reviewed, and their current and potential future food applications are critically discussed.

Ionically crosslinked cellulose nanocrystals by metal nitrates for the preparation of stable emulsions with tunable interface properties.

Biologically extracted cellulose nanocrystals (CNCs) are rod-like and amphiphilic materials with surface-exposed (hydrophilic sites) and hidden (hydrophobic sites) hydroxyl groups. These physicochemical characteristics make CNCs suitable for use as emulsifying agents to stabilize emulsions. Stable oil-in-water emulsions, using sulfated (i.e., -[Formula: see text]) CNCs that were ionically crosslinked with alkaline-earth (i.e., [Formula: see text]) or transition-d-block (i.e., [Formula: see text]) metal cations, were developed without the use of any synthetic surfactants or prior functionalization of pure CNCs with hydrophobic molecules. Various emulsion surface properties such as interfacial tension, surface charge, surface chemistry, as well as rheology were characterized. Ionically crosslinked CNCs (iCNCs) adsorbed at the interface of an oil and water and fortified the emulsion droplets (5-30 µm) against coalescence by lowering the interfacial tension from 65 mN/m (i.e., pure CNC mixture with oil) to 25 mN/m (i.e., iCNC mixture with oil) and reducing zeta potential with surface charge values (-30 mV to -10 mV), ideal to maintain droplet layer assembly at the water-oil interface. This study provided an alternative approach to achieve particle-stabilized and surfactant-free emulsions by using divalent metal nitrates to develop "clean" emulsion-based technologies for applications in many industries from agriculture to food to pharmaceuticals.

Nanomaterial-Based Scaffolds for Tissue Engineering Applications: A Review on Graphene, Carbon Nanotubes and Nanocellulose.

Nanoscale biomaterials have garnered immense interest in the scientific community in the recent decade. This review specifically focuses on the application of three nanomaterials, i.e., graphene and its derivatives (graphene oxide, reduced graphene oxide), carbon nanotubes (CNTs) and nanocellulose (cellulose nanocrystals or CNCs and cellulose nanofibers or CNFs), in regenerating different types of tissues, including skin, cartilage, nerve, muscle and bone. Their excellent inherent (and tunable) physical, chemical, mechanical, electrical, thermal and optical properties make them suitable for a wide range of biomedical applications, including but not limited to diagnostics, therapeutics, biosensing, bioimaging, drug and gene delivery, tissue engineering and regenerative medicine. A state-of-the-art literature review of composite tissue scaffolds fabricated using these nanomaterials is provided, including the unique physicochemical properties and mechanisms that induce cell adhesion, growth, and differentiation into specific tissues. In addition, in vitro and in vivo cytotoxic effects and biodegradation behavior of these nanomaterials are presented. We also discuss challenges and gaps that still exist and need to be addressed in future research before clinical translation of these promising nanomaterials can be realized in a safe, efficacious, and economical manner.

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.).

Maize is an important grain crop in the United States and worldwide. However, maize grain must be processed prior to human consumption. Furthermore, whole grain composition and processing characteristics vary among maize hybrids and can impact the quality of the final processed product. Therefore, in order to produce healthier processed food products from maize, it is necessary to know how to optimize processing parameters for particular sets of germplasm to account for these differences in grain composition and processing characteristics. This includes a better understanding of how current processing techniques impact the nutritional quality of the final processed food product. Here, we describe a microscale protocol that both simulates the processing pipeline to produce cornflakes from large flaking grits and allows for the processing of multiple grain samples simultaneously. The flaking grits, the intermediate processed products, or final processed product, as well as the corn grain itself, can be analyzed for nutritional content as part of a high-throughput analytical pipeline. This procedure was developed specifically for incorporation into a maize breeding research program, and it can be modified for other grain crops. We provide an example of the analysis of insoluble-bound ferulic acid and p-coumaric acid content in maize. Samples were taken at five different processing stages. We demonstrate that sampling can take place at multiple stages during microscale processing, that the processing technique can be utilized in the context of a specialized maize breeding program, and that, in our example, most of the nutritional content was lost during food product processing.

Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases.

Loblolly pine residues have enormous potential to be the raw material for advanced biofuel production due to extensive sources and high cellulose content. Hot water (HW) pretreatment, while being a relatively economical and clean technology for the deconstruction of lignocellulosic biomass, could also inhibit the ensuing enzymatic hydrolysis process because of the production of inhibitors. In this study, we investigated the effect of oligosaccharide fractions purified from HW pre-hydrolyzate of pinewood using centrifugal partition chromatography (CPC) on three recombinant cellulolytic enzymes (E1, CBHI and CBHII), which were expressed in the transgenic corn grain system. The efficiency of recombinant enzymes was measured using either a 4-methylumbelliferyl-ß-D-cellobioside (MUC) or a cellulose-dinitrosalicylic acid (DNS) assay system. The results showed that HW pre-hydrolyzate CPC fractions contain phenolics, furans, and monomeric and oligomeric sugars. Among CPC fractions, oligomers composed of xylan, galactan, and mannan were inhibitory to the three recombinant enzymes and to the commercial cellulase cocktail, reducing the enzymatic efficiency to as low as 10%.

Changes in Phenolic Acid Content in Maize during Food Product Processing.

The notion that many nutrients and beneficial phytochemicals in maize are lost due to food product processing is common, but this has not been studied in detail for the phenolic acids. Information regarding changes in phenolic acid content throughout processing is highly valuable because some phenolic acids are chemopreventive agents of aging-related diseases. It is unknown when and why these changes in phenolic acid content might occur during processing, whether some maize genotypes might be more resistant to processing induced changes in phenolic acid content than other genotypes, or if processing affects the bioavailability of phenolic acids in maize-based food products. For this study, a laboratory-scale processing protocol was developed and used to process whole maize kernels into toasted cornflakes. High-throughput microscale wet-lab analyses were applied to determine the concentrations of soluble and insoluble-bound phenolic acids in samples of grain, three intermediate processing stages, and toasted cornflakes obtained from 12 ex-PVP maize inbreds and seven hybrids. In the grain, insoluble-bound ferulic acid was the most common phenolic acid, followed by insoluble-bound p-coumaric acid and soluble cinnamic acid, a precursor to the phenolic acids. Notably, the ferulic acid content was approximately 1950 µg/g, more than ten-times the concentration of many fruits and vegetables. Processing reduced the content of the phenolic acids regardless of the genotype. Most changes occurred during dry milling due to the removal of the bran. The concentration of bioavailable soluble ferulic and p-coumaric acid increased negligibly due to thermal stresses. Therefore, the current dry milling based processing techniques used to manufacture many maize-based foods, including breakfast cereals, are not conducive for increasing the content of bioavailable phenolics in processed maize food products. This suggests that while maize is an excellent source of phenolics, alternative or complementary processing methods must be developed before this nutritional resource can be utilized.