[Air quality in schools - classroom levels of carbon dioxide (CO2), volatile organic compounds (VOC), aldehydes, endotoxins and cat allergen]. / Raumluftqualität in Schulen - Belastung von Klassenräumen mit Kohlendioxid (CO2), flüchtigen organischen Verbindungen (VOC), Aldehyden, Endotoxinen und Katzenallergenen.

Children are assumed to be more vulnerable to health hazards and spend a large part of their time in schools. To assess the exposure situation in this microenvironment, we evaluated the indoor air quality in winter 2004/5 in 92 classrooms, and in 75 classrooms in summer 2005 in south Bavaria, Germany. Indoor air climate parameters (temperature, relative humidity), carbon dioxide (CO2) and various volatile organic compounds, aldehydes and ketones were measured. Additionally, cat allergen (Fel d1) and endotoxin (LAL-test) were analysed in the settled dust of school rooms. Data on room and building characteristics were collected by use of a standardised form. Only data collected during teaching hours were considered in analysis. The median indoor CO2 concentration in the classrooms ranged in the winter and summer period from 598 to 4 172 ppm and 480 to 1 875 ppm, respectively. While during the winter period in 92% of the classrooms the CO2 daily medians went above 1 000 ppm, the percentage of classrooms with increased CO2 concentration fell to 28% in summer. In winter, in 60% of classes the daily median CO2 concentration exceeded 1 500 ppm, while in summer this threshold was reached by only 9%. A high concentration of CO2 was associated with a high number of pupils, a low room surface area and a low room volume. The levels of total volatile organic compounds (TVOC) in classrooms ranged between 110 and 1 000 microg/m3 (median in winter 345 microg/m3, in summer 260 microg/m3). Acetone, formaldehyde and acetaldehyde were measured in concentrations from 14.0 to 911 microg/m3, from 3.1 to 46.1 microg/m3, and from 2.9 to 78 microg/m3, respectively. The other aldehydes were detected in minor amounts only. The median Fel d1 level in winter was 485 ng/g dust (20 to 45 160 ng/g) and in summer it was 417 ng/g (40-7 470 ng/g). We observed no marked differences between the two sampling periods and between smooth floors and rooms with carpeted floors. No differences were found according to room surface area and room volume. The median endotoxin contents in winter and summer were 19.7 EU/mg dust (6.6 to 154 EU/mg) and 32.2 EU/mg (9.6 to 219 EU/mg), respectively. The levels varied significantly between the sampling periods, but were independent of room surface area, room volume and surface floorings. Overall the results of VOC, aldehydes, ketones and endotoxin indicate, in general, a low exposure level in classrooms. The observed concentrations of cat allergens should be considered as a meaningful exposure route and thus could be tackled within preventive programs.

Exposure of German residents to ethylene and propylene glycol ethers in general and after cleaning scenarios.

Glycol ethers are a class of semi-volatile substances used as solvents in a variety of consumer products like cleaning agents, paints, cosmetics as well as chemical intermediates. We determined 11 metabolites of ethylene and propylene glycol ethers in 44 urine samples of German residents (background level study) and in urine samples of individuals after exposure to glycol ethers during cleaning activities (exposure study). In the study on the background exposure, methoxyacetic acid and phenoxyacetic acid (PhAA) could be detected in each urine sample with median (95th percentile) values of 0.11 mgL(-1) (0.30 mgL(-1)) and 0.80 mgL(-1) (23.6 mgL(-1)), respectively. The other metabolites were found in a limited number of samples or in none. In the exposure study, 5-8 rooms were cleaned with a cleaner containing ethylene glycol monobutyl ether (EGBE), propylene glycol monobutyl ether (PGBE), or ethylene glycol monopropyl ether (EGPE). During cleaning the mean levels in the indoor air were 7.5 mgm(-3) (EGBE), 3.0 mgm(-3) (PGBE), and 3.3 mgm(-3) (EGPE), respectively. The related metabolite levels analysed in the urine of the residents of the rooms at the day of cleaning were 2.4 mgL(-1) for butoxyacetic acid, 0.06 mgL(-1) for 2-butoxypropionic acid, and 2.3 mgL(-1) for n-propoxyacetic acid. Overall, our study indicates that the exposure of the population to glycol ethers is generally low, with the exception of PhAA. Moreover, the results of the cleaning scenarios demonstrate that the use of indoor cleaning agents containing glycol ethers can lead to a detectable internal exposure of residents.

Phthalates and their metabolites in breast milk--results from the Bavarian Monitoring of Breast Milk (BAMBI).

Phthalates have long been used as plasticizers to soften plastic products and, thus, are ubiquitous in modern life. As part of the Bavarian Monitoring of Breast Milk (BAMBI), we aimed to characterize the exposure of infants to phthalates in Germany. Overall, 15 phthalates, including di-2-ethylhexyl phthalate (DEHP), di-n-butyl phthalate (DnBP), di-isobutyl phthalate (DiBP), di-isononyl phthalate (DiNP), three primary metabolites of DEHP [mono-(2-ethylhexyl) phthalate (MEHP), mono-isobutyl phthalate (MiBP), and mono-n-butyl phthalate (MnBP)], and two secondary metabolites of DEHP were analyzed in 78 breast milk samples. We found median concentrations of 3.9 ng/g for DEHP, 0.8 ng/g for DnBP, and 1.2 ng/g for DiBP, while other parent phthalates were found in only some or none of the samples at levels above the limit of quantitation. In infant formula (n=4) we observed mean values of 19.7 ng/g (DEHP), 3.8 ng/g (DnBP), and 3.6 ng/g (DiBP). For MEHP, MiBP, and MnBP, the median values in breast milk were 2.3 µg/l, 11.8 µg/l, and 2.1 µg/l, respectively. The secondary metabolites were not detected in any samples. Using median and 95th percentile values, we estimated an "average" and "high" daily intake for an exclusively breast-fed infant of 0.6 µg/kg body weight (b.w.) and 2.1 µg/kg b.w., respectively, for DEHP, 0.1 µg/kg b.w. and 0.5 µg/kg b.w. for DnBP, and 0.2 µg/kg b.w. and 0.7 µg/kg b.w. for DiBP. For DiNP, intake values were 3.2 µg/kg b.w. and 6.4 µg/kg b.w., respectively, if all values in milk were set half of the detection limit or the detection limit. The above-mentioned "average" and "high" intake values corresponded to only about 2% to 7%, respectively, of the recommended tolerable daily intake. Thus, it is not likely that an infant's exposure to phthalates from breast milk poses any significant health risk. Nevertheless, other sources of phthalates in this vulnerable phase have to be considered. Moreover, it should be noted that for infants nourished with formula, phthalate intake is of the same magnitude or slightly higher (DEHP) than for exclusively breast-fed infants.

Simultaneous quantitation of perfluoroalkyl acids in human serum and breast milk using on-line sample preparation by HPLC column switching coupled to ESI-MS/MS.

A high throughput analytical method using a column switching high-performance liquid chromatography combined with isotope dilution tandem mass spectrometry (column switching-HPLC-MS/MS) was developed to simultaneously quantitate the concentrations of 7 perfluoroalkyl acids (PFAAs) in serum and 3 PFAAs in breast milk samples. The sample preparation includes addition of the isotope-labelled internal standard solution to breast milk and serum, enzymatic hydrolysis and filtration of milk samples, precipitation of proteins and analysis by column switching-HPLC-MS/MS. The limits of quantitation ranged from 0.1 to 0.4 µg/l for serum and 0.02 to 0.15 µg/l for breast milk samples. The method accuracies ranged between 73.2% and 100.2% for the different analytes at two concentrations in PFAAs spiked samples. The validity of the method was confirmed by analysing 20 serum and 20 breast milk samples.