Monitoring of the Sensitivity In Vivo of Plasmodium falciparum to Artemether-Lumefantrine in Mali.

In Mali, since 2007, artemether-lumefantrine has been the first choice against uncomplicated malaria. Despite its effectiveness, a rapid selection of markers of resistance to partner drugs has been documented. This work evaluated the treatment according to the World Health Organization's standard 28-day treatment method. The primary endpoint was the clinical and parasitological response corrected by a polymerase chain reaction. It was more than 99.9 percent, the proportion of patients with anemia significantly decrease compared to baseline (p < 0.001), and no serious events were recorded. Plasmodium falciparum remains sensitive to artemether-lumefantrine in Mali.

[A case study of galen aneurysmal malformation in Bamako]. / Malformation anévrismale de la veine de galien à Bamako à propos d'un cas.

Galen aneurysmal malformation is a rare complex congenital vascular malformation of the brain, (less than 1% of intracranial arteriovenous malformations) characterized by pseudoaneurismal dilation of Galen's ampulla associated with one or several arteriovenous fistulas. In most cases, diagnosis is made in the neonatal or postnatal period, exceptionally in adulthood. We report the case of an 18-year male patient hospitalized for psychomotor retardation. Brain computed tomography (CT) scan using spontaneous contrast (A,B,C) showed voluminous isodense mass in the pineal region behind the third ventricle with peripheral calcifications, compatible with large vein of galen aneurysm. Its anteroposterior diameter was 8.5cm, its transverse diameter was 3cm in the axial plane (C) and its height was 3cm in the sagittal plane (G). Imaging objectified homogeneous contrast enhancement (D,E,F,G,H,I). The mass was associated with dilation of the superior sagittal sinus measured at 2.7cm (A,D), of transverse sinuses measured at 1.5cm to the right and at 1,1cm to the left (B,E). Multiple venous perianeurysmal derivations (I), hydrocephaly and small parenchymal cortical-subcortical calcifications were detected. Embolization can't be performed in Bamako, so the patient is waiting for transfer.

[A case of rupture of voluminous spontaneous subcapsular hematoma of the liver in Bamako]. / Volumineux hématome spontané sous capsulaire hépatique rompu: à propos d'un cas à Bamako.

Rupture of spontaneous subcapsular hematoma of the liver is very rare and therefore it has been rarely described in the literature. It generally occurs among women with underlying liver disease or often, during pregnancy, in women with HELLP syndrome. We here report the case of a 60-year old man with no particular past medical history presenting with large subcapsular haematoma of the liver. He was admitted to the Emergency Department with moderate-intensity right hypochondre pain evolving over the last week, which was aggravated, two days before, by influenza-like illness associating cough, yellowish sputum and sweatings. Clinical examination showed abdominal tenderness on palpation of the right hypochondre and hypogastrium. The remainder of the clinic examination was normal. Laboratory tests showed hemoglobin level 7.8g/dl, MCV: 79.9, reticulocyte count 48.9. computed tomography (CT) scan showed voluminous subcapsular hematoma of the liver measuring 17cm in height, 14cm in cross-sectional diameter and 5cm in thickness and a hepatic nodule measuring 10mm (A) with hemoperitoine of great abundance without active bleeding (B). The patient underwent visceral surgery (laparotomy) with favorable postoperative outcome.

[Kartagener's syndrome found incidentally during assessment of infertile couple: a case study]. / Syndrome de Kartagener de découverte fortuite au cours d'un bilan d'infécondité du couple à propos d'un cas.

Kartagener's syndrome is a rare primitive ciliary dyskinesia (DCP) characterized by a clinical triad: sinusitis, bronchiectasis and complete or incomplete situs inversus. It is a rare congenital autosomal recessive disease. We report a case of Kartagener syndrome in an infertile couple with akinospermia detected using spermogram.

Conservation of HIV-1 T cell epitopes across time and clades: validation of immunogenic HLA-A2 epitopes selected for the GAIA HIV vaccine.

HIV genomic sequence variability has complicated efforts to generate an effective globally relevant vaccine. Regions of the viral genome conserved in sequence and across time may represent the "Achilles' heel" of HIV. In this study, highly conserved T-cell epitopes were selected using immunoinformatics tools combining HLA-A2 supertype binding predictions with relative global conservation. Analysis performed in 2002 on 10,803 HIV-1 sequences, and again in 2009, on 43,822 sequences, yielded 38 HLA-A2 epitopes. These epitopes were experimentally validated for HLA binding and immunogenicity with PBMCs from HIV-infected patients in Providence, Rhode Island, and/or Bamako, Mali. Thirty-five (92%) stimulated an IFNγ response in PBMCs from at least one subject. Eleven of fourteen peptides (79%) were confirmed as HLA-A2 epitopes in both locations. Validation of these HLA-A2 epitopes conserved across time, clades, and geography supports the hypothesis that such epitopes could provide effective coverage of virus diversity and would be appropriate for inclusion in a globally relevant HIV vaccine.