The iPSC secretome is beneficial for in vitro propagation of primary osteoarthritic chondrocytes cell lines.

BACKGROUND: One of the obstacles to autologous chondrocyte implantation (ACI) is obtaining a large quantity of chondrocytes without depletion of their properties. The conditioned medium (CM) from different subpopulations of stem cells (mesenchymal stromal cells (MSC) or induced pluripotent stem cells (iPSC)) could be a gamechanger. MSCs' potential is related to the donor's health and age, which could be omitted when, as a source, iPSCs are used. There is a lack of data regarding their use in the chondrocyte culture expansion. Thus, we wanted to verify whether iPSC-CM could be beneficial for the cell culture of primary chondrocyte cells. METHODS: We added the iPSC-CMs from GPCCi001-A and ND 41658*H cells to the culture of primary chondrocyte cell lines isolated from OA patients (n = 6) for other two passages. The composition of the CM was evaluated using Luminex technology. Then, we analysed the senescence, proliferation rate and using flow cytometry: viability, distribution of cell cycle phases, production of reactive oxygen species (ROS) and double-strand breaks. The cartilage-related markers were evaluated using Western blot and immunofluorescence. Additionally, a three-dimensional cell culture was used to determine the potential to form cartilage particles. RESULTS: iPSC-CM increased proliferation and diminished cell ROS production and senescence. CM influenced the cartilage-related protein expression and promoted the growth of cartilage particles. The cell exposed to CM did not lose the ECM proteins, suggesting the chondroprotective effect for prolonged culture time. CONCLUSION: Our preliminary results suggest a beneficial effect on maintaining chondrocyte biology during in vitro expansion.

Overcoming Resistance to Platinum-Based Drugs in Ovarian Cancer by Salinomycin and Its Derivatives-An In Vitro Study.

Polyether ionophore salinomycin (SAL) and its semi-synthetic derivatives are recognized as very promising anticancer drug candidates due to their activity against various types of cancer cells, including multidrug-resistant populations. Ovarian cancer is the deadliest among gynecologic malignancies, which is connected with the development of chemoresistant forms of the disease in over 70% of patients after initial treatment regimen. Thus, we decided to examine the anticancer properties of SAL and selected SAL derivatives against a series of drug-sensitive (A2780, SK-OV-3) and derived drug-resistant (A2780 CDDP, SK-OV-3 CDDP) ovarian cancer cell lines. Although SAL analogs showed less promising IC50 values than SAL, they were identified as the antitumor agents that significantly overcome the resistance to platinum-based drugs in ovarian cancer, more potent than unmodified SAL and commonly used anticancer drugs-5-fluorouracil, gemcitabine, and cisplatin. Moreover, when compared with SAL used alone, our experiments proved for the first time increased selectivity of SAL-based dual therapy with 5-fluorouracil or gemcitabine, especially towards A2780 cell line. Looking closer at the results, SAL acted synergistically with 5-fluorouracil towards the drug-resistant A2780 cell line. Our results suggest that combinations of SAL with other antineoplastics may become a new therapeutic option for patients with ovarian cancer.

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions.

The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of joint diseases. A number of protocols of in vitro differentiation of iPSC in chondrocytes for regenerative purposes have been recently described. However, in order to use these cells in clinics, the elimination of animal serum and feeder cells is essential. In our study, a strictly defined and controllable protocol was designed for the differentiation of pluripotent stem cells (BG01V, ND 41658*H, GPCCi001-A) in chondrocyte-like cells in serum- and a feeder cell-free system, using the embryoid bodies step. The extension of the protocol and culture conditions (monolayer versus 3D culture) was also tested after the initial 21 days of chondrogenic differentiation. Promotion of the chondrogenic differentiation in 3D culture via the elevated expression of genes related to chondrogenesis was achieved. Using immunofluorescence and immunohistochemistry staining techniques, the increased deposition of the specific extracellular matrix was indicated. As a result, chondrocyte-like cells in the early stages of their differentiation using pellet culture under fully controlled and defined conditions were obtained.

Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro.

Fucoidans have been reported to exert anticancer effects with simultaneous low toxicity against healthy tissue. That correlation was observed in several cancer models, however, it has never been investigated in head and neck cancer before. To magnify the efficacy of conventional therapy, the administration of agents like fucoidan could be beneficial. The aim of this study was to evaluate the anticancer effect of Fucus vesiculosus (FV) extract alone and with co-administration of cisplatin in head and neck squamous cell carcinoma (HNSCC) in vitro. MTT assay results revealed an FV-induced inhibition of proliferation in all tested cell lines (H103, FaDu, KB). Flow cytometric cell cycle analysis showed an FV-induced, dose-dependent arrest in either S/G2 phase (H103, FaDu) or G1 arrest (KB). Furthermore, a dose-dependent gain in apoptotic fraction was observed. Western blot analysis confirmed the induction of apoptosis. A significant dose-dependent increase in reactive oxygen species (ROS) production was revealed in the H103 cell line, while FaDu cells remained unresponsive. On the contrary, an HPV-positive cell line, KB, demonstrated a dose-dependent decrease in ROS synthesis. Moreover, fucoidan enhanced the response to cisplatin (synergistic effect) in all cell lines with the HPV-positive one (KB) being the most sensitive. These results have been confirmed by flow-cytometric apoptosis analysis. In conclusion, we confirmed that fucoidan exhibits anticancer properties against HNSCC, which are manifested by the induction of apoptosis, regulation of ROS production, cell cycle arrest, and inhibition of proliferation.

Correlations between serum adipocytokine concentrations, disease stage, radiological status and total body fat content in the patients with primary knee osteoarthritis.

PURPOSE: The study was designed to investigate whether serum concentrations of leptin, resistin and adiponectin in obese and normal-weight patients with primary knee osteoarthritis (OA) correlate with clinical and radiological stages of the disease and percentage of total body fat. METHODS: Seventy-three patients with knee OA, divided into obese and normal-weight groups, were clinically evaluated according to the Knee Society Score (KSS), and radiologically assessed using Kellgren and Lawrence scale. The percentage of total body fat and some anthropometric data were also given. Serum leptin, resistin and adiponectin concentrations were measured by Elisa and were correlated with the clinical, radiological and anthropometric parameters. RESULTS: Leptin concentrations were significantly higher (p = 0.001) in the obese patients and positively correlated (R = 0.63) with radiologically assessed OA grade, but only in the normal-weight group. Resistin and adiponectin concentrations were identical in obese and normal-weight patients and negatively correlated (R = -0.41) with the clinical status of obese patients. In both groups, percentage of total body fat positively correlated (R = 0.29 and R = 0.53 for obese and normal-weight respectively) with radiologically assessed OA grade. However, no correlations were found with clinical status of the patients. CONCLUSIONS: It was found that in the obese patients with knee OA, increased percentage of total body fat and elevated serum leptin concentration might favour the advancement of clinical but not radiologically assessed changes in the joint structures, while in normal-weight patients it correlates only with radiologically assessed changes but does not affect to an appreciable extent the clinical status of the patients.

Application of cell and biomaterial-based tissue engineering methods in the treatment of cartilage, menisci and ligament injuries.

Over 20 years ago it was realized that the traditional methods of the treatment of injuries to joint components: cartilage, menisci and ligaments, did not give satisfactory results and so there is a need of employing novel, more effective therapeutic techniques. Recent advances in molecular biology, biotechnology and polymer science have led to both the experimental and clinical application of various cell types, adapting their culture conditions in order to ensure a directed differentiation of the cells into a desired cell type, and employing non-toxic and non-immunogenic biomaterial in the treatment of knee joint injuries. In the present review the current state of knowledge regarding novel cell sources, in vitro conditions of cell culture and major important biomaterials, both natural and synthetic, used in cartilage, meniscus and ligament repair by tissue engineering techniques are described, and the assets and drawbacks of their clinical application are critically evaluated.

Insulating Innovative Geopolymer Foams with Natural Fibers and Phase-Change Materials-A Review of Solutions and Research Results.

Geopolymers are synthesized using anthropogenic raw materials and waste from the energy industry. Their preparation necessitates an alkaline activator, which facilitates the dissolution of raw materials and their subsequent binding. At present, geopolymers are considered a promising material with the potential to replace conventional cement-based products. This research investigates foamed geopolymer materials based on fly ash, natural fibers, and phase-change materials. The study utilized three distinct types of fibers and two phase-change materials manufactured by Rubitherm Technologies GmbH of Germany. This paper presents the results of the thermal conductivity coefficient and specific heat tests on the finished foams. Additionally, compressive strength tests were conducted on the samples after 28 days. Natural fibers decreased the insulation parameter by 12%, while PCM enhanced it by up to 6%. The addition of fibers increased the compressive strength by nearly 30%, whereas PCM reduced this by as little as 14%. Natural fibers and phase-change materials had an increased heat capacity by up to 35%. The results demonstrated the material's potential in various industrial sectors, with the primary areas of application being building materials and insulations. The findings illustrate the significant potential of these composites as energetically and environmentally sustainable materials.

The Influence of Diatomite on the Sound Absorption Ability of Composites.

Diatomites are well-known mineral materials formed thousands of years ago from the skeletons of diatoms. They are found in many places around the world and have a wide range of applications. This article presents innovative research related to the possibility of using diatomite as a filler in composites to improve their sound absorption properties. The results of the study of the effect of diatomite processing (calcination) and its degree of fineness on the sound absorption coefficient of thermoplastic composites are presented. Three fractions of diatomite (0 ÷ 0.063 mm; 0.5 ÷ 3 mm; 2 ÷ 5 mm) and its variable mass proportion (0, 25, and 50 wt.%) were used. The composites were made with flax fibers as a reinforcement, polylactide as a matrix, and diatomite as an additional filler. This paper also presents the results of oxide chemical composition, diatomite mineral phase composition, morphology, and thermal conductivity coefficient of all diatomite fractions studied. In addition, the average particle size for diatomite powder was also determined. The most important of the studies was the determination of the acoustic properties of the aforementioned composites. As a result of the tests, it was found that the smallest fraction of diatomite particles and a variant without thermal treatment give the best effect in terms of sound absorption.

Basic research on layered geopolymer composites with insulating materials of natural origin.

New restrictions on carbon dioxide emissions and electricity consumption are currently being introduced around the world. Innovative solutions are being adopted in many countries to reduce CO2 emissions and material and energy consumption. The present work is related to the study of innovative binders based on geopolymers for the production of layered building envelopes. The binders are reinforced with composite bars and containing fibers of natural origin. The natural materials used to produce the samples are completely biodegradable. A 10-mol sodium hydroxide solution with an aqueous solution of sodium silicate was used for alkaline activation of geopolymers. The purpose of the study was to compare and determine the insulating properties of natural fiber-based materials such as coconut mat, jute felt, hemp felt, flax felt, flax wool, hemp wool, flax-jute wool, and to determine the effect of these materials on geopolymer composites, in which 4 layers of natural insulating materials were used, and the composites were reinforced by fiberglass bars. The publication presents the results of physicochemical studies of geopolymerization precursors and natural insulating materials, studies of thermal properties of fibers, mats, felts and wools, morphology of fiber structure and texture, as well as physical and thermal properties of finished multi-layer partitions. The results indicate the great potential of these materials in prefabrication and structural-insulation applications. The fabricated composites using 4 layers of natural fibers showed improved thermal conductivity by as much as 40% (reduced thermal conductivity from 1.36 W/m × K to about 0.8 W/m × K). The work may have future applications in energy-saving and low-carbon construction.

The Influence of the Addition of Basalt Powder on the Properties of Foamed Geopolymers.

Geopolymers are binder materials that are produced by a chemical reaction between silica or aluminum compounds with an alkaline activating solution. Foamed geopolymer materials are increasingly being cited as a viable alternative to popular organic insulation materials. Since the foaming process of geopolymers is difficult to control, and any achievements in improving the performance of such materials are extremely beneficial, this paper presents the effect of the addition of basalt powder on the properties of foamed geopolymers. This paper presents the results of physicochemical studies of fly ash and basalt, as well as mechanical properties, thermal properties, and structure analysis of the finished foams. The scope of the tests included density tests, compressive strength tests, tests of the thermal conductivity coefficient using a plating apparatus, as well as microstructure tests through observations using light and scanning microscopy. Ground basalt was introduced in amounts ranging from 0 to 20% by mass. It was observed that the addition of basalt powder contributes to a reduction in and spheroidization of pores, which directly affect the density and pore morphology of the materials tested. The highest density of 357.3 kg/m3 was characterized by samples with a 5 wt.% basalt powder addition. Their density was 14% higher than the reference sample without basalt powder addition. Samples with 20 wt.% basalt addition had the lowest density, and the density averaged 307.4 kg/m3. Additionally, for the sample containing 5 wt.% basalt powder, the compressive strength exceeded 1.4 MPa, and the thermal conductivity coefficient was 0.1108 W/m × K. The effect of basalt powder in geopolymer foams can vary depending on many factors, such as its chemical composition, grain size, content, and physical properties. The addition of basalt above 10% causes a decrease in the significant properties of the geopolymer.

The Influence of Diatomite Addition on the Properties of Geopolymers Based on Fly Ash and Metakaolin.

Geopolymer materials, considered to be an alternative to Portland cement-based concretes, can be produced from various types of waste aluminosilicate raw materials. This article presents the results of research related to the use of diatomite as an additive in geopolymers. The results of testing geopolymer composites with 1%, 3%, and 5% additions of diatomite with a grain size of 0-0.063 mm after and without thermal treatment were presented. This article presents the physical properties of the diatomite additive, the morphology of diatomite particles SEMs, thermal analysis, and compressive strength test results. In this research, diatomite was treated as a substitute for both fly ash and metakaolin (replaced in amounts of 1 and 3%) and as a substitute for sand introduced as a filler (in this case, 5% of diatomite was added). As a result of this research, it was found that the addition of diatomite instead of the main geopolymerization precursors in amounts of 1 and 3% had a negative impact on the strength properties of geopolymers, as the compressive strength was reduced by up to 28%. The introduction of crushed diatomite instead of sand in an amount of 5% contributed to an increase in strength of up to 24%.

Application of Industrial Waste Materials by Alkaline Activation for Use as Geopolymer Binders.

The purpose of this study is to synthesize geopolymer binders as an environmentally friendly alternative to conventional cement using available local raw materials. Waste materials such as chalcedonite (Ch), amphibolite (A), fly ash from lignite combustion (PB), and diatomite dust (D) calcined at 900 °C were used to produce geopolymer binders. Metakaolin (M) was used as an additional modifier for binders based on waste materials. The base materials were subjected to fluorescence X-ray fluorescence (XRF) analysis and X-ray diffractometry (XRD) to determine chemical and phase composition. A laser particle size analysis was also performed. The various mixtures of raw materials were activated with a 10 M solution of NaOH and sodium water glass and then annealed for 24 h at 60 °C. The produced geopolymer binders were conditioned for 28 days under laboratory conditions and then subjected to microstructural analysis (SEM) and flexural and compressive strength tests. The best compressive strength results were obtained by the Ch + PB samples-more than 57 MPa, while the lowest results were obtained by the Ch + D+A + M samples-more than 20 MPa. On the other hand, as a result of the flexural strength tests, the highest flexural results were obtained by D + A + M + PB binders-more than 12 MPa, and the lowest values were obtained by binders based on Ch + D+A + M-about 4.8 MPa.

Possibilities of Using Geopolymers in Welding Processes and Protection against High Temperatures.

Geopolymer materials have long been known for their competitive properties against traditional construction materials. Their special features include high resistance to elevated temperatures and good fire resistance. They are typically used as insulating materials at temperatures not exceeding 100 °C (because they can achieve a thermal conductivity coefficient of 0.060 W/m × K or less under these conditions). Still, they can also be used as thermal insulation at temperatures exceeding 1000 °C. One technology that uses very high temperatures is metal welding technology, where temperatures often exceed as many as 3000 °C. Geopolymers, due to their properties, can also be an interesting new alternative in various welding applications. This paper presents the preliminary results of pot-proofing the resistance of geopolymers to temperatures exceeding 3000 °C. Test results of a foamed geopolymer insulating a steel substrate are presented, and a geopolymer mold for thermite rail welding was made and realistically tested. The results confirmed the feasibility of using cast geopolymer molds for thermite welding of railroad rails. The geopolymer material performed well during the test and no cracks or other damage occurred. The following article presents the potential of using geopolymer materials for welding applications.

Cyclic Load Impact Assessment of Long-Term Properties in Compression to Steel and Polyvinyl Alcohol Fibre-Reinforced Geopolymer Composites.

This study investigates the cyclic load application impact on fly-ash-based geopolymer composites that are reinforced with a low amount of fibre reinforcement. For reinforcement purposes, polyvinyl alcohol and steel fibres are used. For testing purposes, four geopolymer composite mixes were made, three of which had fibre reinforcement. Simultaneously, specimens were tested for shrinkage, static-load-induced creep, and cyclic-load-induced creep. For static and cyclic creep testing, specimens were loaded with 20% of their strength. For cyclic creep testing, load application and release cycles were seven days long. When each cycle was introduced, the load was applied in steps. Necessary load application or unloading lasted for 5 min and consisted of four steps, each 25% of the necessary load. From the long-term static and cyclic creep tests, it was seen that only the plain specimens showed that static creep strains are within cyclic creep strains. For all the other specimens, the static load was higher than the cyclic-load-induced creep amplitude. Also, 1% polyvinyl alcohol fibre-reinforced specimens showed the most elastic characteristics under cyclic load, and 1% steel fibre-reinforced specimens appeared to be the most resistant to the cyclic load introduction.

Advanced Geopolymer-Based Composites for Antimicrobial Application.

In most studies about geopolymeric materials used in construction, the antibacterial properties of the building materials are treated as secondary features. Today, antimicrobial properties are a key feature in many building applications. The main objective of this article is to summarize the state-of-the-art in the area of design, development, and applications of nanoparticles as additives to geopolymer composites used in construction to improve their physical mechanical properties and induce a potential antibacterial effect, protecting them against alkali-resistant bacteria. On the basis of the literature and authors' experience, the most important methods of obtaining especially the porous geopolymers, of nanomaterials used as additives, with potential antibacterial effect but also the potential mechanism of action against bacterial development were presented. The main findings show that using graphene oxide (GO) in geopolymer composites, but also other nanoparticles such as silver (Ag), zinc oxide (ZnO), silica (SiO2), titanium dioxide (TiO2), copper (Cu) as additives, is an effective way to induce a potential antibacterial effect and to improve the physical and mechanical properties in building materials.

Conditioned Medium - Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management?

BACKGROUND: The approaches currently used in osteoarthritis (OA) are mainly short-term solutions with unsatisfactory outcomes. Cell-based therapies are still controversial (in terms of the sources of cells and the results) and require strict culture protocol, quality control, and may have side-effects. A distinct population of stromal cells has an interesting secretome composition that is underrated and commonly ends up as biological waste. Their unique properties could be used to improve the existing techniques due to protective and anti-ageing properties. SCOPE OF REVIEW: In this review, we seek to outline the advantages of the use of conditioned media (CM) and exosomes, which render them superior to other cell-based methods, and to summarise current information on the composition of CM and their effect on chondrocytes. MAJOR CONCLUSIONS: CM are obtainable from a variety of mesenchymal stromal cell (MSC) sources, such as adipose tissue, bone marrow and umbilical cord, which is significant to their composition. The components present in CMs include proteins, cytokines, growth factors, chemokines, lipids and ncRNA with a variety of functions. In most in vitro and in vivo studies CM from MSCs had a beneficial effect in enhance processes associated with chondrocyte OA pathomechanism. GENERAL SIGNIFICANCE: This review summarises the information available in the literature on the function of components most commonly detected in MSC-conditioned media, as well as the effect of CM on OA chondrocytes in in vitro culture. It also highlights the need to standardise protocols for obtaining CM, and to conduct clinical trials to transfer the effects obtained in vitro to human subjects.

The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use?

The development of induced pluripotent stem cells has brought unlimited possibilities to the field of regenerative medicine. This could be ideal for treating osteoarthritis and other skeletal diseases, because the current procedures tend to be short-term solutions. The usage of induced pluripotent stem cells in the cell-based regeneration of cartilage damages could replace or improve on the current techniques. The patient's specific non-invasive collection of tissue for reprogramming purposes could also create a platform for drug screening and disease modelling for an overview of distinct skeletal abnormalities. In this review, we seek to summarise the latest achievements in the chondrogenic differentiation of pluripotent stem cells for regenerative purposes and disease modelling.

MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression.

Uveal melanoma (UM) is a rare type of malignancy that originates from melanocytes in the choroid, iris and the eye's ciliary body. Biomarkers for early detection and progression of UM, especially the molecular traits governing the development of metastasis, are still not available in clinical practice. One extensively studied components of liquid biopsies are extracellular vesicles. Due to their unique molecular cargo, they can contribute to early cancer development and at the same time carry markers for disease onset and progression. For characterisation of the miRNA profiles present in circulating serum-derived exosomes of patients with diagnosed primary and metastatic UM, we have analyzed the miRNA cargos using next-generation sequencing followed by RT-qPCR validation in a cohort of patients (control n = 20; primary n = 9; metastatic n = 11). Nine miRNAs differentiating these patient groups have been established. We show that hsa-miR-144-5p and hsa-miR-191-5p are the most promising biomarker candidates, allowing the categorization of patients into local and advanced UM. Additionally, the comparison of miRNA expression levels in exosomes derived from UM patients with those derived from healthy donors revealed that hsa-miR-191-5p, -223-3p, -483-5p, -203a has the potential to be used as an early marker for the presence of UM. This pilot study reveals that miRNAs extracted from circulating exosomes could be exploited as potential biomarkers in UM diagnosis and, more importantly, for indicating metastatic spread.

3D Printing of Concrete-Geopolymer Hybrids.

In recent years, 3D concrete printing technology has been developing dynamically. Intensive research is still being carried out on the composition of the materials dedicated to innovative 3D printing solutions. Here, for the first time, concrete-geopolymer hybrids produced with 3D printing technology and dedicated environmentally friendly building construction are presented. The concrete-geopolymer hybrids consisting of 95% concrete and 5% geopolymer based on fly ash or metakaolin were compared to standard concrete. Moreover, 3D printed samples were compared with the samples of the same composition but prepared by the conventional method of casting into molds. The phase composition, water leachability, compressive, and flexural strength in the parallel and perpendicular directions to the printing direction, and fire resistance followed by compressive strength were evaluated. Concrete-geopolymer hybrids were shown to contain a lower content of hazardous compounds in leaches than concrete samples. The concentration of toxic metals did not exceed the limit values indicated in the Council Decision 2003/33/EC; therefore, the materials were classified as environmentally neutral. The different forms of Si/Al in fly ash and metakaolin resulted in the various potentials for geopolymerization processes, and finally influenced the densification of the hybrids and the potential for immobilization of toxic elements. Although the compressive strength of concrete was approximately 40% higher for cast samples than for 3D printed ones, for the hybrids, the trend was the opposite. The addition of fly ash to concrete resulted in a 20% higher compressive strength compared to an analogous hybrid containing the addition of metakaolin. The compressive strength was 7-10% higher provided the samples were tested in the parallel direction to the Z-axis of the printout. The sample compressive strength of 24-43 MPa decreased to 8-19 MPa after the fire resistance tests as a result of moisture evaporation, weight loss, thermal deformation, and crack development. Importantly, the residual compressive strength of the hybrid samples was 1.5- to 2- fold higher than the concrete samples. Therefore, it can be concluded that the addition of geopolymer to the concrete improved the fire resistance of the samples.

Properties of 3D Printed Concrete-Geopolymer Hybrids Reinforced with Aramid Roving.

Three-dimensional concrete printing (3DCP) is an innovative technology that can lead to breakthrough modifications of production processes in the construction industry. The paper presents for the first time the possibility of 3D printing concrete-geopolymer hybrids reinforced with aramid roving. Reference concrete samples and concrete-geopolymer hybrids composed of 95% concrete and 5% geopolymer based on fly ash or metakaolin were produced. The properties of the samples without reinforcement and samples with 0.5% (wt.) aramid roving were compared. The frost resistance tests, UV radiation resistance, and thermal conductivity were evaluated for samples that were 3D-printed or produced by the conventional casting method. Compressive strength tests were carried out for each sample exposed to freeze-thaw cycles and UV radiation. It was observed that after the frost resistance test, the samples produced by the 3D printing technology had a minor decrease in strength properties compared to the samples made by casting. Moreover, the thermal conductivity coefficient was higher for concrete-geopolymer hybrids than concrete reinforced with aramid roving.