RESUMO
Minimum inhibitory concentrations (MICs) of the antifungal agent voriconazole were determined using the Etest and compared with those of amphotericin B, itraconazole and fluconazole using 1986 clinical isolates of Candida spp. Voriconazole MICs were also compared with those of amphotericin B and itraconazole using 391 clinical isolates of Aspergillus spp. Voriconazole was found to have more potent activity and lower MIC values than amphotericin B, itraconazole and fluconazole against C. albicans, C. tropicalis, C. parapsilosis and C. kefyr. Against C. glabrata and C. krusei, voriconazole was more active than either of the other two azole antifungals but had similar activity to amphotericin B. For species of Aspergillus, MIC values of voriconazole were lower than those of amphotericin B and itraconazole against A. fumigatus and A. flavus, and were similar to those of amphotericin B against A. niger. Against A. terreus, MIC values for voriconazole and itraconazole were similar. A. terreus is known to be resistant to amphotericin B, and this was reflected in higher MIC values compared with those of voriconazole and itraconazole. Voriconazole therefore compares very favourably with other antifungal agents against a large number of clinical isolates of Candida and Aspergillus spp.
Assuntos
Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Candida/efeitos dos fármacos , Pirimidinas/farmacologia , Triazóis/farmacologia , Anfotericina B/farmacologia , Aspergillus/classificação , Candida/classificação , Farmacorresistência Fúngica , Humanos , Itraconazol/farmacologia , Testes de Sensibilidade Microbiana/métodos , Testes de Sensibilidade Microbiana/estatística & dados numéricos , VoriconazolRESUMO
Trichoderma species are filamentous fungi that were previously considered to be culture contaminants. We report 2 well-documented cases of invasive Trichoderma infections, and we comprehensively review the literature on this topic. Trichoderma species are mainly responsible for continuous ambulatory peritoneal dialysis-associated peritonitis (7 cases) and invasive infections in immunocompromised patients (9 cases) with a hematologic malignancy or solid-organ transplant. Definitive diagnosis is difficult to achieve because of the lack of specific diagnosis tools. Species identification can benefit from a molecular approach. Trichoderma longibrachiatum is the most common species involved in these infections. Regardless of the type of infection, the prognosis was poor, with 8 deaths among 18 cases. This may be partially because of the resistance of these organisms to the majority of available antifungal agents, including amphotericin B. Trichoderma species now should be added to the growing list of emerging filamentous fungal pathogens.
Assuntos
Antifúngicos/farmacologia , Resistência Microbiana a Medicamentos/fisiologia , Micoses/microbiologia , Trichoderma/efeitos dos fármacos , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Micoses/mortalidadeRESUMO
Two cases of invasive aspergillosis (IA) in immunocompetent patients with a fulminant fatal outcome are reported. Both patients were elderly and had a history of chronic lung disease treated with prolonged inhaled corticosteroids and a short course of systemic corticosteroids. They presented with dyspnea and fever, their respiratory function deteriorated rapidly, and they died 7 days after admission. Aspergillus fumigatus was cultured from respiratory samples. IA was confirmed in one case by necropsy that showed diffuse bilateral necrotizing pneumonitis and myocarditis. In the other case, IA diagnosis was established by thoracic CT scan plus detection of Aspergillus antigen in two blood samples. These two cases demonstrate that short-term corticosteroid therapy in immunocompetent patients with underlying chronic lung conditions is a risk factor for IA, and that its evolution can be fulminant.
Assuntos
Corticosteroides/uso terapêutico , Aspergilose Broncopulmonar Alérgica/microbiologia , Aspergillus fumigatus/crescimento & desenvolvimento , Broncopatias/tratamento farmacológico , Administração por Inalação , Corticosteroides/administração & dosagem , Corticosteroides/efeitos adversos , Idoso , Idoso de 80 Anos ou mais , Aspergilose Broncopulmonar Alérgica/tratamento farmacológico , Evolução Fatal , Feminino , Humanos , Imunocompetência , MasculinoRESUMO
The eosinophilic meningitis due to Angiostrongylus cantonensis is well known on Madagascar and on Mauritius Island, but was never described on Reunion Island. Two cases have been lately oberved in Doctor Jay's department at Gabriel Martin's hospital. Local achatines were dissected and contained larvae which, when absorbed by rats, were found in there brains ten days later. These larvae belong certainly to Angiostrongylus genus, but the species has not yet been found with certitude. Lately infested rats are passing through the normal laps of time for adult maturation until they will be sacrified for further examinations.
Assuntos
Meningite/epidemiologia , Strongyloidea/isolamento & purificação , Adulto , Animais , Líquido Cefalorraquidiano/citologia , Criança , Vetores de Doenças , Eosinófilos , Humanos , Ilhas do Oceano Índico , Masculino , Meningite/parasitologia , Ratos , CaramujosRESUMO
The authors describe the pathological aspects of muscles of three patients infected with Trichinella murrelli. Biopsies were carried out at various intervals. Six weeks after infection, the muscular larvae were not encapsulated whereas encapsulation was seen 10 weeks after infection. Six years after infection, the larvae were still alive in a nurse cell surrounded by a very thick capsule. Fourteen years after infection, cuticular larvae remnants were seen in degenerating nurse cells. The late encapsulation of Trichinella murrelli in human muscles could explain some clinical differences noticed during the outbreak during which these three patients were infected.
Assuntos
Músculo Esquelético/patologia , Músculo Esquelético/parasitologia , Trichinella/isolamento & purificação , Triquinelose/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Biópsia , Seguimentos , Humanos , Larva , Masculino , Dor , Fatores de Tempo , Triquinelose/fisiopatologiaRESUMO
Pneumocystis carinii pneumonia (PCP) is a well known opportunistic infection in Systemic Lupus Erythematosus (SLE) patients with lymphopenia and treated with corticosteroid or cytotoxics agents. We report a new case of PCP in an untreated SLE with severe lymphopenia. We discuss the origin of lymphopenia in SLE, lymphopenia as a risk factor of Pneumocystis carinii infection, and safety precautions to take.
Assuntos
Lúpus Eritematoso Sistêmico/complicações , Pneumonia por Pneumocystis/etiologia , Feminino , Humanos , Lúpus Eritematoso Sistêmico/imunologia , Pessoa de Meia-Idade , Pneumonia por Pneumocystis/imunologiaAssuntos
Aspergilose/epidemiologia , Transplante de Coração , Transplante de Coração-Pulmão , Transplante de Rim , Transplante de Pulmão , Complicações Pós-Operatórias/epidemiologia , Adulto , Idoso , Anfotericina B/uso terapêutico , Aspergilose/mortalidade , Aspergilose/prevenção & controle , Causas de Morte , Feminino , Transplante de Coração/mortalidade , Transplante de Coração-Pulmão/mortalidade , Humanos , Incidência , Transplante de Rim/mortalidade , Transplante de Pulmão/mortalidade , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de RiscoRESUMO
Fluconazole is a new antifungal drug which is now very often used for the treatment of yeasts infections: mucocutaneous candidosis, systemic candidosis and cryptococcosis. Some resistance seems to occur in clinical practice. It turns out now that it must be necessary to test the drug susceptibility in vitro of the yeast strains isolated from patients. Unfortunately, a current laboratory technique to test this agent doesn't exist. In this work a useful method for Minimal inhibitory concentration (MIC) evaluation in a commercial available broth medium, and a technique for testing fluconazole disks on an agar medium are studied. 150 strains of different yeasts species are tested. A regression curve is performed to correlate the two methods.
Assuntos
Candida albicans/efeitos dos fármacos , Candida/efeitos dos fármacos , Fluconazol/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Trichosporon/efeitos dos fármacos , Antifúngicos/farmacologia , Cryptococcus neoformans/efeitos dos fármacos , Relação Dose-Resposta a Droga , Técnicas In VitroRESUMO
After a coprologic survey made at Araracuara, a village in colombian Amazonia showing high prevalences of nematodosis, 83 villages (including 55 children below twelve) were given Flubendazole during three consecutive days. The total dose was 600 mg. for children below 12, and 900 mg. for adults. The product efficiency was 88% for Trichuris, 82% for Hookworm, 77% for Ascaris and 43% for Strongyloïdes. The tolerance was excellent in all cases, in spite of heavy parasital infection. The Flubendazole's effect seems to be particularly interesting by its polyvalency.
Assuntos
Anti-Helmínticos/uso terapêutico , Antinematódeos/uso terapêutico , Benzimidazóis/uso terapêutico , Mebendazol/uso terapêutico , Infecções por Nematoides/tratamento farmacológico , Ancilostomíase/tratamento farmacológico , Ascaríase/tratamento farmacológico , Ascaris , Mebendazol/análogos & derivados , Estrongiloidíase/tratamento farmacológico , TrichuroideaRESUMO
The detection of some cases of eosinophilic meningitis suggest that Angiostrongylus cantonensis is present in Reunion Island. Larvae of this nematode have been isolated after dissection of Achatina fulica and have been used to infest laboratory rats. 30 days later, the dissection of these animals shows the presence of young worms in the brain and in the pulmonary arteries. 45 days later, first-stage larvae are recovered from the feces of animals. Now, the strain is maintained in Biomphalaria glabrata and in the rat.
Assuntos
Metastrongyloidea/isolamento & purificação , Animais , Humanos , Ilhas do Oceano Índico , Meningite/parasitologia , Infecções por Nematoides/diagnóstico , Infecções por Nematoides/epidemiologia , RatosRESUMO
The action of minocycline has been studied in comparison with tetracycline on 52 strains of "C. albicans" by M.I.C. on Agar medium and by the dellophane transfer. A fungistatic activity was observed above 4 mug/ml, and a fungicidal activity above 1000 mug/ml, whereas tetracycline "in vitro" proved to be ineffective.
Assuntos
Candida albicans/efeitos dos fármacos , Minociclina/farmacologia , Tetraciclinas/farmacologia , Candidíase Bucal/microbiologia , Humanos , Tetraciclina/farmacologiaRESUMO
The increase of organ transplantations during the last decades conjointly with the prescription of heavy immunosuppressive drugs, has led to an increased incidence of new invasive aspergillosis (IA). This study is a report of the Broussais Hospital experience from 1968 to 1993 on kidney, heart and heart and lungs transplantations. It concerns 21 IA cases. Incidence was 0.5% for kidney, 4.5% for heart and 18% for heart and lungs transplantations. The most important risk factors were the increase of immunosuppressive therapy (66% of the cases), neutropenia (19%), and renovation of the hospital wards (36%). Lung was the most frequent site of infection (95% of the cases), clinical symptoms were no significant. Diagnosis procedures were realised on biopsy (23%) and on bronchoalveolar lavage (66%). Usual amphotericin B treatment was disappointing: mortality rate of 77%, the liposomal preparation of the drug seemed to be more efficient: mortality rate of 50%. Itraconazole appeared to be used in succession with a careful adaptation of posology. Prophylactic amphotericin B in a local way (sprays and aerosols) led to a good efficiency jointly with the patient isolation during constructions in the hospital area.
Assuntos
Aspergilose/etiologia , Transplante de Coração/efeitos adversos , Transplante de Coração-Pulmão/efeitos adversos , Transplante de Rim/efeitos adversos , Transplante de Pulmão/efeitos adversos , Adolescente , Adulto , Idoso , Anfotericina B/uso terapêutico , Aspergilose/diagnóstico , Aspergilose/mortalidade , Aspergilose/terapia , Criança , Feminino , Humanos , Tolerância Imunológica , Itraconazol/uso terapêutico , Pneumopatias Fúngicas/diagnóstico , Pneumopatias Fúngicas/etiologia , Pneumopatias Fúngicas/mortalidade , Pneumopatias Fúngicas/terapia , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Fatores de RiscoRESUMO
Toxoplasmosis is a life-threatening disease in heart- or lung transplant recipients that can result either from the reactivation of a latent infection or from an organ-transmitted infection. The diagnosis of acute toxoplasmosis is easy in cases of seroconversion following a mismatch. However, when the recipient is Toxoplasma-seropositive before transplantation, usual serological techniques do not allow the differentiation between endogenous and organ-related reinfection. The aim of this study was to determine whether western blotting could contribute to this differentiation. Sequential sera from two heart- and one liver- and lung transplant patients whose anti-Toxoplasma antibody titers strongly increased after transplantation, were analyzed by western blotting. Neosynthesized IgG were observed on blots incubated with the sera from two patients who had received transplants from Toxoplasma-seropositive donors, whereas no neosynthesized IgG was detected on blots from the patient who had received a transplant from a Toxoplasma-seronegative donor. Our results suggest that the detection of neosynthesized IgG in the recipient may be related to the recognition of a new parasite strain possibly brought by the transplant from a Toxoplasma-seropositive donor.
Assuntos
Anticorpos Antiprotozoários/biossíntese , Transplante de Coração/efeitos adversos , Imunoglobulina G/biossíntese , Transplante de Pulmão/efeitos adversos , Complicações Pós-Operatórias/imunologia , Toxoplasmose/transmissão , Adolescente , Idoso , Anticorpos Antiprotozoários/sangue , Western Blotting , Ensaio de Imunoadsorção Enzimática , Evolução Fatal , Feminino , Coração/parasitologia , Transplante de Coração/imunologia , Humanos , Imunoglobulina G/sangue , Transplante de Fígado , Pulmão/parasitologia , Transplante de Pulmão/imunologia , Masculino , Pessoa de Meia-Idade , Risco , Estudos Soroepidemiológicos , Doadores de Tecidos , Toxoplasmose/sangue , Toxoplasmose/imunologiaRESUMO
We compared the capability of rapid enzyme immunoassay (EIA) to detect antiamoebic antibodies during hepatic amoebiasis with those of indirect hemagglutination and latex agglutination. EIA is simple to perform and rapid (20 min) and does not require any special equipment (optical reading is sufficient). EIA of 143 sera (including 43 from patients with proven hepatic amoebic abscess, 33 from patients with other hepatic disorders and/or parasitic infections, and 67 from healthy individuals) yielded a specificity, a sensitivity, and positive and negative predictive values of 100, 93, 100, and 97.1, respectively. This test could thus be considered another valuable tool for the diagnosis of hepatic amoebiasis.
Assuntos
Anticorpos Antiprotozoários/sangue , Entamoeba histolytica/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Abscesso Hepático Amebiano/diagnóstico , Animais , Ensaio de Imunoadsorção Enzimática/economia , Estudos de Avaliação como Assunto , Testes de Hemaglutinação/economia , Humanos , Testes de Fixação do Látex/economia , Valor Preditivo dos Testes , Sensibilidade e EspecificidadeRESUMO
Fusarium spp. have emerged as major opportunistic fungal agents. Since new antifungal agents exhibit variable activity against Fusarium isolates depending on the species, rapid identification at the species level is required. Conventional culture methods are difficult, fastidious, and sometimes inconclusive. In this work, we sequenced a 440-bp fragment encoding the 28S rRNA from 33 Fusarium isolates belonging to six Fusarium species associated with human infections. The data were then analyzed by the neighbor-joining method. By using distance matrix analysis and constructing the phylogram, we could easily distinguish the different species for all but one isolate. The method also allowed differentiation between the closely related genera Acremonium and Cylindrocarpon. In contrast to the case with conventional methods, the results could be obtained within 48 h from a 3-day culture and are independent of mycologist experience, making this method rapid and reliable for identification of Fusarium species isolated from patients.
Assuntos
Fusarium/genética , Micoses/microbiologia , RNA Fúngico/genética , RNA Ribossômico 28S/genética , Sequência de Bases , Primers do DNA/genética , DNA Fúngico/genética , DNA Ribossômico/genética , Fusarium/classificação , Fusarium/isolamento & purificação , Humanos , Micologia/métodos , Filogenia , Especificidade da EspécieRESUMO
The evolution of the classes of specific immunoglobulins (IgG, IgM, IgA, IgE) of 40 cases of human trichinosis was followed over a nine month period by means of an indirect immunofluorescence test (IF) and ELISA. Specific IgG and IgM appear between 4 and 6 weeks after infection and were still demonstrable after 9 months (respectively in 87.5% and 38.5% of the patients with IF, in 85% and 87.5% with ELISA). Specific IgA were mainly seen on the 2nd month and specific IgE were detected in 37.5% of the patients on the 4th month. In this outbreak, specific antibodies seem to appear later than in a second outbreak observed a few weeks later.
Assuntos
Surtos de Doenças , Imunoglobulina A/análise , Imunoglobulina E/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Triquinelose/imunologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Seguimentos , França , Humanos , Pessoa de Meia-Idade , Fatores de Tempo , Triquinelose/epidemiologiaRESUMO
Eleven days after double lung transplantation for cystic fibrosis, an 18-year-old patient developed a disseminated Fusarium solani infection with tricuspid valve endocarditis. This infection occurred under fluconazole and immunosuppressive therapy with cyclosporin, prednisone and azathioprine, with a normal leucocyte count. Liposomal amphotericin B allowed blood culture negativation. The patient died from a bacterial septic shock.