First record of the fungal genus Neodevriesia Quaedvl. & Crous (Ascomycota, Dothideomycetes, Neodevriesiaceae) isolated from scleractinian corals of Perhentian Islands, Malaysia.

Fungal species members of the genus Neodevriesia have been known to occur in marine environments. This report documents the first record of the fungal genus Neodevriesia isolated from scleractinian corals. Three isolated strains were identified from a phylogenetic tree that was constructed, based on the nuclear ribosomal internal transcribed spacer and partial large subunit (ITS + LSU) DNA sequences. Isolates were closely related to both Neodevriesiashakazului (Crous) Crous and Neodevriesiaqueenslandica (Crous, R.G. Shivas & McTaggart) Crous, but formed a distinct clade with strong support that implies a potentially genetic variant of a known species or even a novel species. These findings contribute to the fungal diversity checklist in Malaysia and knowledge about marine fungi associated with scleractinian corals.

Molecular diversity and assemblages of coral symbionts (Symbiodiniaceae) in diverse scleractinian coral species.

The scleractinian coral-associated symbiotic algae Symbiodiniaceae plays an important role in bleaching tolerance and coral resilience. In this study, coral-associated Symbiodiniaceae communities of 14 reef sites of Perhentian and Redang Islands Marine Parks (Malaysia, South China Sea) were characterized using the high-throughput next-generation amplicon sequencing on the ITS2 rDNA marker to inventory the Symbiodiniaceae diversity from a healthy tropical reef system and to generate a baseline for future studies. A total of 64 coral-Symbiodiniaceae associations were characterized in 18 genera (10 families) of scleractinian corals using the SymPortal analytical framework. The results revealed the predominance of Symbiodiniaceae genera Cladocopium (average 82%) and Durusdinium (18%), while Symbiodinium, Breviolum, Fugacium, and Gerakladium were found as minor groups (<0.01%). Of the 39 Cladocopium and Durusdinium major ITS2 sequences, 14 were considered dominant/sub-dominant, with C3u as the predominant type (63.3%), followed by D1 (15%), C27 (10.1%), and C15 (6.9%). A total of 19 and 13 Cladocopium and Durusdinium ITS2-type profiles were detected across the coral species, respectively. Symbiodiniaceae diversity and richness recorded in this study were higher when compared to other reefs in the proximity. With the increasing coral-Symbiodiniaceae associations archived, the database would provide a baseline to assess the changes of Symbiodiniaceae communities in the coral hosts and to explore the potential adaptive roles of this coral-algal association.