RESUMO
Motivation: In the new release of pymzML (v2.0), we have optimized the speed of this established tool for mass spectrometry data analysis to adapt to increasing amounts of data in mass spectrometry. Thus, we integrated faster libraries for numerical calculations, improved data retrieving algorithms and have optimized the source code. Importantly, to adapt to rapidly growing file sizes, we developed a generalizable compression scheme for very fast random access and applied this concept to mzML files to retrieve spectral data. Results: pymzML performs at par with established C programs when it comes to processing times. However, it offers the versatility of a scripting language, while adding unprecedented fast random access to compressed files. Additionally, we designed our compression scheme in such a general way that it can be applied to any field where fast random access to large data blocks in compressed files is desired. Availability and implementation: pymzML is freely available on https://github.com/pymzML/pymzML under GPL license. pymzML requires Python3.4+ and optionally numpy. Documentation available on http://pymzml.readthedocs.io.
Assuntos
Compressão de Dados/métodos , Espectrometria de Massas/métodos , Software , Algoritmos , Proteômica/métodosRESUMO
POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer formed on the PORE (ATTTGAAATGCAAAT) can recruit the transcriptional coactivator OBF-1, whereas POU dimers formed on the consensus MORE (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) fail to interact. An interaction with OBF-1 is precluded since the same Oct-1 residues that form the MORE dimerization interface are also used for OBF-1/Oct-1 interactions on the PORE. Our findings provide a paradigm of how specific POU dimer assemblies can differentially recruit a coregulatory activity with distinct transcriptional readouts.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Oligonucleotídeos/metabolismo , Transativadores/metabolismo , Fatores de Transcrição/metabolismo , Motivos de Aminoácidos , Animais , Sequência de Bases , Linhagem Celular , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Dimerização , Regulação da Expressão Gênica , Genes Reporter , Fator C1 de Célula Hospedeira , Humanos , Camundongos , Modelos Moleculares , Dados de Sequência Molecular , Fator 1 de Transcrição de Octâmero , Fator 2 de Transcrição de Octâmero , Fator 3 de Transcrição de Octâmero , Fator 6 de Transcrição de Octâmero , Oligonucleotídeos/genética , Regiões Promotoras Genéticas , Ligação Proteica/genética , Estrutura Terciária de Proteína , Análise de Sequência , Transativadores/genética , Fator de Transcrição Pit-1 , Fatores de Transcrição/química , Fatores de Transcrição/genética , Transcrição Gênica , TransfecçãoRESUMO
Health promotion for schoolchildren requires a medical staff due to the multitude of functions. The introduced calculatory basis for an appropriate allocation of personnel allows to meet various requirements in different regions of the FRG.
Assuntos
Educação em Saúde , Serviços de Saúde Escolar , Adolescente , Criança , Pré-Escolar , Feminino , Alemanha , Promoção da Saúde , Humanos , Imunização , Masculino , Admissão e Escalonamento de Pessoal , Exame Físico , Serviços de Enfermagem Escolar , Medicina Esportiva , Recursos HumanosRESUMO
Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of approximately 96% of the approximately 2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (approximately 6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that approximately 47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.