RESUMO
Strain DCS523 was isolated from the branch tissue of Daphniphyllum longeracemosum and determined to be a Penicillium sp. according to the ITS sequence analysis. The extracts from the PDA solid fermentation media of Penicillium sp. DCS523 were purified to give two new chroman derivatives as well as six known compounds. Based on their spectral data the new compounds were identified as (Z)-6-acetyl- 3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methylchroman-2-one and 6-acetyl-2α,5- dihydroxy-2-(2-hydroxypropyl)- 3α,8-dimethylchroman, respectively.
Assuntos
Cromanos/isolamento & purificação , Penicillium/química , Sequência de Bases , Cromanos/química , Cromatografia Líquida de Alta Pressão , Primers do DNA , Espectroscopia de Ressonância Magnética , Penicillium/genética , RNA Ribossômico 5,8S/genética , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria UltravioletaRESUMO
The strain DC01 was isolated from the branch tissue of Daphniphyllum longeracemosum and determined to be a member of Phomopsis according to the ITS sequence analysis. The extracts from the PDA solid fermentation media of Phomopsis sp. DC01 were purified and three compounds including one new phenol compound were obtained. The new compound was identified to be (E )-7-(2-hydroxy-4-(hydroxymethyl)phenyl)-2-methyloct-6-enoic acid (1) based on 1-D NMR, 2-D NMR and HR-ESI data.