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1.
Plant Biotechnol J ; 2024 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-38497586

RESUMO

Adzuki bean (Vigna angularis) is an important legume crop cultivated in over 30 countries worldwide. We developed a high-quality chromosome-level reference genome of adzuki bean cultivar Jingnong6 by combining PacBio Sequel long-read sequencing with short-read and Hi-C technologies. The assembled genome covers 97.8% of the adzuki bean genome with a contig N50 of approximately 16 Mb and a total of 32 738 protein-coding genes. We also generated a comprehensive genome variation map of adzuki bean by whole-genome resequencing (WGRS) of 322 diverse adzuki beans accessions including both wild and cultivated. Furthermore, we have conducted comparative genomics and a genome-wide association study (GWAS) on key agricultural traits to investigate the evolution and domestication. GWAS identified several candidate genes, including VaCycA3;1, VaHB15, VaANR1 and VaBm, that exhibited significant associations with domestication traits. Furthermore, we conducted functional analyses on the roles of VaANR1 and VaBm in regulating seed coat colour. We provided evidence for the highest genetic diversity of wild adzuki (Vigna angularis var. nipponensis) in China with the presence of the most original wild adzuki bean, and the occurrence of domestication process facilitating transition from wild to cultigen. The present study elucidates the genetic basis of adzuki bean domestication traits and provides crucial genomic resources to support future breeding efforts in adzuki bean.

2.
BMC Genomics ; 24(1): 195, 2023 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-37046231

RESUMO

BACKGROUND: In plants, RNA silencing is an important conserved mechanism to regulate gene expression and combat against abiotic and biotic stresses. Dicer-like (DCL) and Argonaute (AGO) proteins and RNA-dependent RNA polymerase (RDR) are the core elements involved in gene silencing and their gene families have been explored in many plants. However, these genes and their responses to stresses have not yet been well characterized in adzuki bean. RESULTS: A total of 11 AGO, 7 DCL and 6 RDR proteins were identified, and phylogenetic analyses of these proteins showed that they clustered into six, four and four clades respectively. The expression patterns of these genes in susceptible or resistant adzuki bean cultivars challenged with drought, bean common mosaic virus and Podosphaera xanthii infections were further validated by quantitative RT-PCR. The different responses of these proteins under abiotic and biotic stresses indicated their specialized regulatory mechanisms. CONCLUSIONS: In this study, 24 genes of the DCL, AGO and RDR gene families in adzuki bean were identified, and the sequence characterization, structure of the encoded proteins, evolutionary relationship with orthologues in other legumes and gene expression patterns under drought and biotic stresses were primarily explored, which enriched our understanding of these genes in adzuki bean. Our findings provide a foundation for the comparative genomic analyses of RNA silencing elements in legume plants and further new insights into the functional complexity of RNA silencing in the response to various stresses in adzuki bean.


Assuntos
Fabaceae , Vigna , Vigna/genética , Filogenia , Interferência de RNA , Secas , Genoma de Planta , Fabaceae/genética , Fabaceae/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
3.
Insect Mol Biol ; 31(5): 659-670, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-35690916

RESUMO

As an intermediate molecule in the Insulin/Insulin-like growth factor signalling pathway (IIS), the insulin receptor (IR) plays vital roles linking nutritional signals to the downstream regulation of metabolic homeostasis, development, metamorphosis, reproduction and stress responses. In the present study, we describe the molecular characteristics of IR in the cosmopolitan fruit boring pest, Grapholita molesta, and its predicted posttranscription regulator miR-982490, and elucidate its regulatory roles in glucolipid homeostasis and metamorphosis. Phylogenetic and domain analyses indicate that lepidopteran IRs normally cluster within families, and that four main domains are conserved in GmIR and those of other Lepidoptera. Bio-informatic prediction, synchronic expression profile evaluation and dual luciferase reporter assays indicated negative regulation of GmIR by miR-982490. Injection of miR-982490 agomir into fifth instar larvae yielded effects similar to dsGmIR injection, resulting in enhanced levels of trehalose and triglyceride in haemolymph, and reduced pupation success and pupal weight, both of which could be rescued by co-injection of dsGmIR and miR-982490 antagomir. We infer that GmIR regulates glucolipid homeostasis and affects G. molesta metamorphosis via interactions with its posttranscriptional regulator miR-982490. This study expands our understanding of the regulatory network of IIS in insect nutritional homeostasis and development.


Assuntos
MicroRNAs , Mariposas , Animais , Frutas , Homeostase , Larva/genética , MicroRNAs/genética , Filogenia , Receptor de Insulina/genética
4.
Zhongguo Zhong Yao Za Zhi ; 47(9): 2480-2490, 2022 May.
Artigo em Zh | MEDLINE | ID: mdl-35531704

RESUMO

The fingerprint of Boenninghausenia albiflora var. albiflora was established by ultra performance liquid chromatography(UPLC), and the content of 12 active components including chlorogenic acid was determined. Multivariate statistical analysis was used to explore the indicator components of B. albiflora var. albiflora and a comprehensive evaluation system was created for the quality of B. albiflora var. albiflora. In this study, 33 batches of B. albiflora var. albiflora with different sources were collected and studied, and the UPLC fingerprint of B. albiflora var. albiflora was developed. There were 37 common peaks, of which 12 components were identified, and the content of these 12 components was measured. In combination of the common peaks and the content of chemical components, multivariate statistical analysis was performed, and the results showed that 6 components [daphnoretin, isoimperatorin, astragalin, imperatorin, neochlorogenic acid, and isoquercitrin(weight coefficient>0.1)] were selected as chemical markers for the quality of B. albiflora var. albiflora. Technique for order of preference by similarity to ideal solution(TOPSIS) analysis and chemometrics revealed that the quality of S32, S28 and S29 were superior, while that of S12, S7 and S16 were inferior. The quality evaluation method of B. albiflora var. albiflora constructed in this study was accurate and reliable, with simpleness and easiness to operate. It is suggested that the 6 above-mentioned active components could be used as indicator components for quality control of B. albiflora var. al-biflora. The samples were harvested during the flowering and fruiting period, which is from the beginning of July to the end of August.


Assuntos
Medicamentos de Ervas Chinesas , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida , Medicamentos de Ervas Chinesas/química , Análise Multivariada , Controle de Qualidade
5.
BMC Genomics ; 22(1): 124, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33602135

RESUMO

BACKGROUND: Micrococcus luteus is a group of actinobacteria that is widely used in biotechnology and is being thought as an emerging nosocomial pathogen. With one of the smallest genomes of free-living actinobacteria, it is found in a wide range of environments, but intraspecies genetic diversity and adaptation strategies to various environments remain unclear. Here, comparative genomics, phylogenomics, and genome-wide association studies were used to investigate the genomic diversity, evolutionary history, and the potential ecological differentiation of the species. RESULTS: High-quality genomes of 66 M. luteus strains were downloaded from the NCBI GenBank database and core and pan-genome analysis revealed a considerable intraspecies heterogeneity. Phylogenomic analysis, gene content comparison, and average nucleotide identity calculation consistently indicated that the species has diverged into three well-differentiated clades. Population structure analysis further suggested the existence of an unknown ancestor or the fourth, yet unsampled, clade. Reconstruction of gene gain/loss events along the evolutionary history revealed both early events that contributed to the inter-clade divergence and recent events leading to the intra-clade diversity. We also found convincing evidence that recombination has played a key role in the evolutionary process of the species, with upto two-thirds of the core genes having been affected by recombination. Furthermore, distribution of mammal-associated strains (including pathogens) on the phylogenetic tree suggested that the last common ancestor had a free-living lifestyle, and a few recently diverged lineages have developed a mammal-associated lifestyle separately. Consistently, genome-wide association analysis revealed that mammal-associated strains from different lineages shared genes functionally relevant to the host-associated lifestyle, indicating a recent ecological adaption to the new host-associated habitats. CONCLUSIONS: These results revealed high intraspecies genomic diversity of M. luteus and highlighted that gene gain/loss events and extensive recombination events played key roles in the genome evolution. Our study also indicated that, as a free-living species, some lineages have recently developed or are developing a mammal-associated lifestyle. This study provides insights into the mechanisms that drive the genome evolution and adaption to various environments of a bacterial species.


Assuntos
Genoma Bacteriano , Micrococcus luteus , Animais , Evolução Molecular , Variação Genética , Estudo de Associação Genômica Ampla , Genômica , Micrococcus luteus/genética , Filogenia , Recombinação Genética
6.
Appl Environ Microbiol ; 85(7)2019 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30658977

RESUMO

Deciphering the genomic variation that represents microevolutionary processes toward species divergence is key to understanding microbial speciation, which has long been under debate. Streptomycetes are filamentous bacteria that are ubiquitous in nature and the richest source of antibiotics; however, their speciation processes remain unknown. To tackle this issue, we performed a comprehensive population genomics analysis on Streptomyces albidoflavus residing in different habitats and with a worldwide distribution and identified and characterized the foundational changes within the species. We detected three well-defined phylogenomic clades, of which clades I and III mainly contained free-living (soil/marine) and insect-associated strains, respectively, and clade II had a mixed origin. By performing genome-wide association studies (GWAS), we identified a number of genetic variants associated with free-living or entomic (denoting or relating to insects) habitats in both the accessory and core genomes. These variants contributed collectively to the population structure and had annotated or confirmed functions that likely facilitate differential adaptation of the species. In addition, we detected higher levels of homologous recombination within each clade and in the free-living group than within the whole species and in the entomic group. A subset of the insect-associated strains (clade III) showed a relatively independent evolutionary trajectory with more symbiosis-favorable genes but little genetic interchange with the other lineages. Our results demonstrate that ecological adaptation promotes genetic differentiation in S. albidoflavus, suggesting a model of ecological speciation with gene flow in streptomycetes.IMPORTANCE Species are the fundamental units of ecology and evolution, and speciation leads to the astounding diversity of life on Earth. Studying speciation is thus of great significance to understand, protect, and exploit biodiversity, but it is a challenge in the microbial world. In this study, using population genomics, we placed Streptomyces albidoflavus strains in a spectrum of speciation and showed that the genetic differences between phylogenomic clusters evolved mainly by environmental selection and gene-specific sweeps. These findings highlight the role of ecology in structuring recombining bacterial species, making a step toward a deeper understanding of microbial speciation. Our results also raise concerns of an underrated microbial diversity at the intraspecies level, which can be utilized for mining of ecologically relevant natural products.


Assuntos
Adaptação Fisiológica/genética , Ecologia , Evolução Molecular , Metagenômica , Streptomyces/genética , Streptomyces/fisiologia , Animais , Biodiversidade , Ecossistema , Fluxo Gênico , Genes Bacterianos , Variação Genética , Estudo de Associação Genômica Ampla , Genótipo , Recombinação Homóloga , Insetos/microbiologia , Família Multigênica/genética , Ácido N-Acetilneuramínico/metabolismo , Compostos Orgânicos/metabolismo , Filogenia , Polimorfismo de Nucleotídeo Único , Microbiologia do Solo , Streptomyces/classificação , Streptomyces/isolamento & purificação , Microbiologia da Água
7.
Proc Natl Acad Sci U S A ; 112(43): 13213-8, 2015 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-26460024

RESUMO

Adzuki bean (Vigna angularis), an important legume crop, is grown in more than 30 countries of the world. The seed of adzuki bean, as an important source of starch, digestible protein, mineral elements, and vitamins, is widely used foods for at least a billion people. Here, we generated a high-quality draft genome sequence of adzuki bean by whole-genome shotgun sequencing. The assembled contig sequences reached to 450 Mb (83% of the genome) with an N50 of 38 kb, and the total scaffold sequences were 466.7 Mb with an N50 of 1.29 Mb. Of them, 372.9 Mb of scaffold sequences were assigned to the 11 chromosomes of adzuki bean by using a single nucleotide polymorphism genetic map. A total of 34,183 protein-coding genes were predicted. Functional analysis revealed that significant differences in starch and fat content between adzuki bean and soybean were likely due to transcriptional abundance, rather than copy number variations, of the genes related to starch and oil synthesis. We detected strong selection signals in domestication by the population analysis of 50 accessions including 11 wild, 11 semiwild, 17 landraces, and 11 improved varieties. In addition, the semiwild accessions were illuminated to have a closer relationship to the cultigen accessions than the wild type, suggesting that the semiwild adzuki bean might be a preliminary landrace and play some roles in the adzuki bean domestication. The genome sequence of adzuki bean will facilitate the identification of agronomically important genes and accelerate the improvement of adzuki bean.


Assuntos
Evolução Biológica , Produtos Agrícolas/genética , Fabaceae/química , Fabaceae/genética , Regulação da Expressão Gênica de Plantas/genética , Genoma de Planta/genética , Sequência de Bases , Perfilação da Expressão Gênica , Lipídeos/análise , Lipídeos/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Análise de Sequência de DNA , Amido/análise , Amido/genética
8.
J Environ Sci Health B ; 52(10): 736-744, 2017 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-28937878

RESUMO

The degradation of chlorpyrifos (CP) by an endophytic bacterial strain (HJY) isolated from Chinese chives (Allium tuberosum Rottl. ex Spreng) was investigated. Strain HJY was identified as Sphingomonas sp. based on morphological, physiological, and biochemical tests and a 16S rDNA sequence analysis. Approximately 96% of 20 mg L-1 CP was degraded by strain HJY over 15 days in liquid minimal salts medium (MSM). The CP degradation rate could also be increased by glucose supplementation. The optimal conditions for the removal of 20 mg L-1 CP by strain HJY in MSM were 2% inoculum density, pH 6.0, and 30-35°C. The CP degradation rate constant and half-life were 0.2136 ± 0.0063 d-1 and 3.2451 ± 0.0975 d, respectively, under these conditions, but were raised to 0.7961 ± 0.1925 d-1 and 0.8707 ± 0.3079 d with 1% glucose supplementation. The detection of metabolic products and screening for degrading genes indicated that O,O-diethyl O-3,5,6-trichloropyridinol was the major degradation product from CP, while it was likely that some functional genes were undetected and the mechanism responsible for CP degradation by strain HJY remained unknown. Strain HJY is potentially useful for the reduction of CP residues in Chinese chives and may be used for the in situ phytoremediation of CP.


Assuntos
Cebolinha-Francesa/microbiologia , Clorpirifos/metabolismo , Sphingomonas/metabolismo , Biodegradação Ambiental , DNA Ribossômico , Endófitos/metabolismo , Concentração de Íons de Hidrogênio , Sphingomonas/genética , Sphingomonas/isolamento & purificação
9.
Ann Plast Surg ; 75(6): 610-4, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25180948

RESUMO

The objective of this study was to evaluate a total parotidectomy performed through a face-lift incision integrated with a temporal fascia flap. We have accomplished a group of 40 cases of total parotidectomy from July 2008 to May 2013. Twenty-two cases accepted a modified performance which combined rhytidectomy incision with temporal fascia flap. The other 18 cases were fulfilled by Blair incision and no reconstruction of parotid bed as control. The patients were followed up every 6 months. In the interviews, the assessment of the operation from patients was recorded. The cosmetic gratification, presence or absence of gustatory flushing or sweating, and functional reversion of facial nerve and great auricular nerve were surveyed by 3 investigators. The criteria that integrated the subjective with objective items were stipulated for evaluation. Gustatory sweating had been identified in 0% and 44% of patients of the testing and control group, respectively. The average scale of the experimental and control group postoperatively was 7.89 and 5.93 individually. The difference of the average scale between testing and control group presented statistical significance. The author's technique is either aesthetically satisfying or efficacious to prevention of gustatory sweating in total parotidectomy.


Assuntos
Fasciotomia , Glândula Parótida/cirurgia , Complicações Pós-Operatórias/prevenção & controle , Retalhos Cirúrgicos , Sudorese Gustativa/prevenção & controle , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Ritidoplastia , Sudorese Gustativa/etiologia , Resultado do Tratamento
10.
Bull Environ Contam Toxicol ; 95(3): 401-6, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25904088

RESUMO

The sorption behavior of chlorantraniliprole (CAP) by biochar and effect of soil extracts on sorptivity in soil-biochar systems were examined. The results showed that biochar amendment could enhance the sorption of CAP in soils. The values of K F increased significantly when the soils were amended with 0.5 % BC850, which were from 1.54 to 196.5. The indigenous sorptivity of biochar was suppressed after it was applied to the soils. The degree of biochar sorptivity attenuation in different soil-biochar systems varied with the properties of soil water soluble matters. Sorption of CAP by biochar from the five soil extracts was found to be lower than that from a CaCl2 solution. The calculated K d values at C w of 0.01 mg kg(-1) for biochar sorption of CAP from CaCl2 solution were 21.4-26.6 times of that from soil extracts. Aging of biochar in soil extract reduced CAP sorption by up to 85 %.


Assuntos
Carvão Vegetal/química , Poluentes do Solo/química , ortoaminobenzoatos/química , Adsorção , Solo/química
11.
ISME J ; 18(1)2024 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-38366066

RESUMO

Microorganisms living in soil maintain intricate interactions among themselves, forming the soil microbiota that influences the rhizosphere microbiome and plant growth. However, the mechanisms underlying the soil microbial interactions remain unclear. Streptomyces and Mesorhizobium are commonly found in soil and serve as plant growth-promoting rhizobacteria (PGPR). Here, we identified an unprecedented interaction between the colonies of red-soil-derived Streptomyces sp. FXJ1.4098 and Mesorhizobium sp. BAC0120 and referred to it as "proximity-based defensive mutualism (PBDM)." We found that metabolite-mediated iron competition and sharing between the two microorganisms were responsible for PBDM. Streptomyces sp. FXJ1.4098 produced a highly diffusible siderophore, desferrioxamine, which made iron unavailable to co-cultured Mesorhizobium sp. BAC0120, thereby inhibiting its growth. Streptomyces sp. FXJ1.4098 also released poorly diffusible iron-porphyrin complexes, which could be utilized by Mesorhizobium sp. BAC0120, thereby restoring the growth of nearby Mesorhizobium sp. BAC0120. Furthermore, in ternary interactions, the PBDM strategy contributed to the protection of Mesorhizobium sp. BAC0120 close to Streptomyces sp. FXJ1.4098 from other microbial competitors, resulting in the coexistence of these two PGPR. A scale-up pairwise interaction screening suggested that the PBDM strategy may be common between Mesorhizobium and red-soil-derived Streptomyces. These results demonstrate the key role of iron in complex microbial interactions and provide novel insights into the coexistence of PGPR in soil.


Assuntos
Mesorhizobium , Streptomyces , Simbiose , Streptomyces/genética , Ferro , Mesorhizobium/genética , Rizosfera , Solo , Microbiologia do Solo , Raízes de Plantas
12.
Front Microbiol ; 15: 1400096, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38912353

RESUMO

Introduction: Clinical significance of coagulase-negative staphylococci (CoNS) has been gradually acknowledged in both healthcare and clinical research, but approaches for their precise discrimination at the species level remain scarce. The current study aimed to evaluate the association of CoNS with orthopedic infections, where accurate and prompt identification of etiology is crucial for appropriate diagnosis and treatment decision-making. Methods: A 16S rRNA-based quantitative PCR (qPCR) assay was developed for the detection of Staphylococcus genus and two panels of 3-plex qPCR assays for further differentiation of six CoNS species with remarkable clinical significance, including S. epidermidis, S. haemolyticus, S. simulans, S. hominis, S. capitis, and S. caprae. All the assays exhibited excellent analytical performance. ΔCq (quantification cycle) between 16S rRNA and CoNS species-specific targets was established to determine the primary CoNS. These methods were applied to detect CoNS in wound samples from orthopedic patients with and without infection. Results and discussion: Overall, CoNS were detected in 17.8% (21/118) of patients with clinically suspected infection and in 9.8% (12/123) of patients without any infection symptom (p < 0.05). Moreover, the association with infection was found to be bacterial quantity dependent. S. epidermidis was identified as the predominant species, followed by S. simulans, S. haemolyticus, and S. hominis. Male sex, open injury, trauma, and lower extremity were determined as risk factors for CoNS infections. CoNS-positive patients had significantly longer hospitalization duration (20 days (15, 33) versus 13 days (7, 22) for Staphylococcus-negative patients, p = 0.003), which could be a considerable burden for healthcare and individual patients. Considering the complex characteristics and devastating consequences of orthopedic infections, further expanding the detection scope for CoNS may be pursued to better understand the etiology of orthopedic infections and to improve therapeutic strategies.

13.
Pathogens ; 13(3)2024 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-38535573

RESUMO

Campylobacter species, especially C. jejuni and C. coli, are the main zoonotic bacteria causing human gastroenteritis. A variety of Campylobacter species has been reported in wild birds, posing a potential avian-human transmission pathway. Currently, there has been little surveillance data on Campylobacter carriage in migratory birds in China. In the current work, fresh fecal droppings from individual migratory birds were collected at four bird wintering/stopover sites in China from May 2020 to March 2021. Nucleic acid was extracted and tested for Campylobacter with PCR-based methods. Overall, 73.8% (329/446) of the samples were positive for Campylobacter, demonstrating location and bird host specificity. Further speciation revealed the presence of C. jejuni, C. coli, C. lari, C. volucris, and an uncharacterized species, which all harbored a variety of virulence factors. Phylogenetic analysis performed on concatenated 16S rRNA-atpA-groEL genes elucidated their genetic relationship, demonstrating both inter- and intra-species diversity. The wide distribution and high diversity of Campylobacter spp. detected in migratory birds in China indicated potential transmission across territories. The existence of virulence factors in all of these species highlighted their public health importance and the necessity of monitoring and controlling Campylobacter and other pathogens carried by migratory birds.

14.
Front Cell Infect Microbiol ; 14: 1394352, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38938882

RESUMO

Introduction: Accurate identification of the etiology of orthopedic infection is very important for correct and timely clinical management, but it has been poorly studied. In the current study we explored the association of multiple bacterial pathogens with orthopedic infection. Methods: Hospitalized orthopedic patients were enrolled in a rural hospital in Qingdao, China. Wound or exudate swab samples were collected and tested for twelve bacterial pathogens with both culture and multiplex real time PCR. Results and discussion: A total of 349 hospitalized orthopedic patients were enrolled including 193 cases presenting infection manifestations upon admission and 156 with no sign of infection. Orthopedic infection patients were mainly male (72.5%) with more lengthy hospital stay (median 15 days). At least one pathogen was detected in 42.5% (82/193) of patients with infection while 7.1% (11/156) in the patients without infection (P < 0.001). S. aureus was the most prevalent causative pathogen (15.5%). Quantity dependent pathogen association with infection was observed, particularly for P. aeruginosa and K. pneumoniae, possibly indicating subclinical infection. Most of the patients with detected pathogens had a previous history of orthopedic surgery (odds ratio 2.8, P = 0.038). Pathogen specific clinical manifestations were characterized. Multiplex qPCR, because of its high sensitivity, superior specificity, and powerful quantification could be utilized in combination with culture to guide antimicrobial therapy and track the progression of orthopedic infection during treatment.


Assuntos
Reação em Cadeia da Polimerase Multiplex , Humanos , Feminino , Masculino , Pessoa de Meia-Idade , Idoso , China/epidemiologia , Adulto , Bactérias/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Infecções Bacterianas/microbiologia , Infecções Bacterianas/diagnóstico , Hospitalização , Idoso de 80 Anos ou mais , Reação em Cadeia da Polimerase em Tempo Real , Hospitais Rurais
15.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 44(1): 72-5, 2013 Jan.
Artigo em Zh | MEDLINE | ID: mdl-23600214

RESUMO

OBJECTIVE: To compare the predictive performances of glomerular filtration rate (GFR) prediction formulae based on serum crea level in diabetic patients and to determine their clinical application values. METHODS: Fasting serum crea of 125 diabetic patients in Chengdu were detected. 99mTc-DTPA clearance rate was used as the reference test (rGFR). The eGFR was obtained using different formulae (aMDRD and CKD-EPI formula in this study). The bias, precision, accuracy and diagnostic sensitivity of the two formulae were compared. ROC curve analyses were performed with different diagnostic boundary points [rGFR < S90 or < 60 mL/(min x 1.73(2))]. RESULTS: Significant differences between eGFR and rGFR were found with both formulae (P < 0.05). There were no significant differences in the predict performance between the two formulae in terms of bias, precision and accuracy within 30% and 50%. Two cases with higher than 90 mL/(min x 1.732) rGFR were identified as outliers using aMDRD formula according to the eGFR-rGFR scatterplot analysis. The ROC curve analysis showed that the AUC were in the range between 0.8 and 0.9. The AUC approached 0.9 when proteinuria was taken into consideration for both formulae, and this involved a significant improvement (P < 0.05). CONCLUSION: Both aMDRD and CKD-EPI formulae predict eGFR accurately in diabetic patients. However, the CKD-EPI formula may have better stability in predicting eGFR in patients at an early stage of renal injury. Combined use of proteinuria may improve the diagnostic performance of eGFR prediction formulae. [Key words] Diabetes Glomerular filtration rate aMDRD CKD-EPI


Assuntos
Diabetes Mellitus/diagnóstico , Taxa de Filtração Glomerular , Diagnóstico Diferencial , Humanos , Testes de Função Renal , Valor Preditivo dos Testes , Proteinúria , Curva ROC
16.
PLoS One ; 18(1): e0278750, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36652458

RESUMO

Delayed production mode has been adopted by an increasing number of process production enterprises as a method to realize mass customization of multi-products. This paper used the convolutional neural network-long short-term memory artificial neural network algorithm (C-LSTM) in data mining technology to analyze and determine factors that have an impact on delayed production mode in the internal and external production and operation of enterprises. Combined with the actual production situation of iron and steel enterprises, a quantitative model of the delayed production was constructed. Lastly, data from a large iron and steel enterprise with good operation was used to verify the validity of the proposed model and analyze key influencing factors. According to the research, in scenarios of considering PDP alone, considering CODP alone, considering both PDP and CODP, considering PDP and CODP and using data mining technology to model, the matching degree of these methods with the actual situation of the enterprise is 31.8%, 61.4%, 71.6% and 86.6%, respectively. The numerical analysis results of the model based on data mining technology show that in delayed production, when customer service level improves or the delay penalty coefficient increases, the optimal locations of the product differentiation point (PDP) and customer order decoupling point (CODP) move toward the end of production, and the total cost increases gradually. When the difference in production cost or benefit of early delivery between the candidate locations of PDP and CODP is small, optimal locations of PDP and CODP are close to the beginning of the general and dedicated production processes. With an increase of cost difference or early delivery benefit, the optimal locations of PDP and CODP jumped to the end stage of the general and dedicated production processes, and the total cost begins to decrease.


Assuntos
Ferro , Aço , Algoritmos , Redes Neurais de Computação
17.
Front Microbiol ; 14: 1267152, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37840734

RESUMO

Sialic acids comprise a varied group of nine-carbon amino sugars found mostly in humans and other higher metazoans, playing major roles in cell interactions with external environments as well as other cells. Microbial sialic acid catabolism (SAC) has long been considered a virulence determinant, and appears to be mainly the purview of pathogenic and commensal bacterial species associated with eukaryotic hosts. Here, we used 2,521 (pre-)assembled metagenomes to evaluate the distribution of SAC in microbial communities from diverse ecosystems and human body parts. Our results demonstrated that microorganisms possessing SAC globally existed in non-host associated environments, although much less frequently than in mammal hosts. We also showed that the ecological significance and taxonomic diversity of microbial SAC have so far been largely underestimated. Phylogenetic analysis revealed a strong signal of horizontal gene transfer among distinct taxa and habitats, and also suggested a specific ecological pressure and a relatively independent evolution history in environmental communities. Our study expanded the known diversity of microbial SAC, and has provided the backbone for further studies on its ecological roles and potential pathogenesis.

18.
Cell Biol Int ; 36(11): 1049-53, 2012 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-22900901

RESUMO

We have explored the optimal seeding density and timing for transplantation of the tissue-engineered bone with BMMSCs (bone marrow mesenchymal stem cells) and PDPB (partially deproteinized bone) in vitro. Rabbit BMMSCs of different densities were seeded into PDPB generated from fresh pig vertebrates to reconstruct tissue-engineered bone in vitro. Adhesion and proliferation of BMMSCs were analysed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay from which growth curves of BMMSCs on the PDPB materials were generated. The data show that BMMSCs began to adhere to PDPB after 24 h of primary culture, all groups reaching peak growth on the 6th day, after which the value of A decreased gradually and reached a plateau phase. The optimal BMMSCs seeding density of 5 × 10(6)/ml achieved an excellent adhesion and proliferation activity on PDPB. In summary, the best cell seeding density of constructing tissue-engineered bone with BMMSCs in vitro is 5 × 10(6)/ml, the optimal timing to transplant is the 6th day.


Assuntos
Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Osteogênese , Engenharia Tecidual/métodos , Animais , Osso e Ossos/citologia , Adesão Celular , Contagem de Células , Proliferação de Células , Células Cultivadas , Meios de Cultivo Condicionados , Cultura Primária de Células/métodos , Coelhos , Suínos , Sais de Tetrazólio , Tiazóis
19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(1): 104-7, 117, 2012 Jan.
Artigo em Zh | MEDLINE | ID: mdl-22455143

RESUMO

OBJECTIVE: To develop an estimating formula for glomerular filtration Rate (GFR) based on serum cystatin C in patients with chronic kidney disease (CKD). METHODS: Clinical characteristics of 242 CKD patients were collected. The patients were randomly divided into modeling group and model validation group. The rGFR obtained from 99mTc-DTPA clearance rate was used as a reference value of GFR. s-cystatin C was detected by latex enhanced immunoturbidimetric method. Preliminary linear regression analysis followed by multiple linear regression were performed to investigate the association between s-cystatin C and rGFR. The validity of the estimation formula was tested in the model validation group in comparison with Hoek formula and Orebro formula. RESULTS: With standardised countdown conversion, s-cystatin showed linear correlation with rGFR, with a correlation coefficient of 0.773. The multiple correlation coefficient, determination coefficient, adjusted R square and std. error of the estimation model were 0.863, 0.745, 0.742, and 0.207, respectively. The residuals P-P probability plot analysis showed that the model residuals fitted into normal distribution with homogeneity of variance. Theeformula was: eGFR = 67/s-cystatin C +3. No significant difference was found between the distribution of eGFR and rGFR. Our formula had an accuracy of 30% and 50%, which were no less than those obtained from Hoek formula and Orebro formula. The new formula also had acceptable bias and high precision. The Bland-Altman analysis and ROC curve analysis showed good applicability of the new formula. CONCLUSION: The GFR prediction formula we established has a good prediction performance as comparised with other formulae, which could be used in measuring GFR in CKD patients.


Assuntos
Cistatina C/sangue , Taxa de Filtração Glomerular/fisiologia , Nefropatias/fisiopatologia , Insuficiência Renal Crônica/fisiopatologia , Adulto , Idoso , Doença Crônica , Feminino , Humanos , Nefropatias/metabolismo , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Insuficiência Renal Crônica/metabolismo , Reprodutibilidade dos Testes
20.
Microbiol Spectr ; 10(1): e0238021, 2022 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-35019771

RESUMO

Sialic acids are present in humans and other metazoans, playing essential roles in physiological and pathological processes. Commensal and pathogenic bacteria have evolved the capacity to utilize sialic acids as nutrient and energy sources. However, in some actinobacteria, sialic acid catabolism (SAC) is associated with free-living populations. To unravel the distribution and evolutionary history of SAC in the phylum Actinobacteria, we analyzed the presence and diversity of the putative SAC gene cluster (nan) in 7,180 high-quality, nonredundant actinobacterial genomes that covered 1,969 species. The results showed that ∼13% of actinobacterial species had the potential to utilize sialic acids, with 45 species capable of anhydro-SAC, all except two of them through the canonical pathway. These species belonged to 20 orders and 81 genera, with ∼36% of them from four genera, Actinomyces, Bifidobacterium, Corynebacterium, and Streptomyces. Moreover, ∼40% of the nan-positive species are free living. Phylogenetic analysis of the key nan genes, nanA, nanK, and nanE, revealed a strong signal of horizontal gene transfer (HGT), accompanied with vertical inheritance and gene loss. This evolutionary pattern led to high diversity and differential distribution of nan among actinobacterial taxa and might cause the cluster to spread to some free-living species while losing in some host-associated species. The evolution of SAC in actinobacteria probably represents the evolution of certain kinds of noncore bacterial functions for environmental adaptation and lifestyle switch, in which HGT plays a dominant role. IMPORTANCE Sialic acids play essential roles in the physiology of humans and other metazoan animals, and microbial sialic acid catabolism (SAC) is one of the processes critical for pathogenesis. To date, microbial SAC is studied mainly in commensals and pathogens, while its distribution in free-living microbes and evolutionary pathway remain largely unexplored. Here, by examining all actinobacterial genomes available, we demonstrate that putative SAC is present in a small proportion of actinobacterial species, of which, however, ∼40% are free-living species. We also reveal remarkable difference in the distribution of SAC among actinobacterial taxa and high diversity of the putative SAC gene clusters. HGT plays a significant role in the evolution of SAC, accompanied with vertical inheritance and gene loss. Our results provide a comprehensive and systematic picture of the distribution and evolutionary history of SAC in actinobacteria, expanding the current knowledge on bacterial adaptation and diversification.


Assuntos
Actinobacteria/genética , Actinobacteria/metabolismo , Evolução Molecular , Ácido N-Acetilneuramínico/metabolismo , Actinobacteria/classificação , Actinobacteria/isolamento & purificação , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Transferência Genética Horizontal , Genoma Bacteriano , Humanos , Família Multigênica , Filogenia
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