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It is important to determine the relationship between the concentration of chlorophyll a (Chla) and the inherent optical properties (IOPs) of ocean water to develop optical models and algorithms that characterize the biogeochemical properties and estimate biological pumping and carbon flux in this environment. However, previous studies reported relatively large variations in the particulate backscattering coefficient (bbp(λ)) and Chla from more eutrophic high-latitude waters to clear oligotrophic waters, especially in oligotrophic oceanic areas where these two variables have little covariation. In this study, we examined the variability of bbp(λ) and Chla in the euphotic layer in oligotrophic areas of the tropical Western Pacific Ocean and determined the sources of these variations by reassessment of in-situ measurements and the biogeochemical-argo (BGC-Argo) database. Our findings identified covariation of bbp(λ) and Chla in the water column below the deep Chla maximum (DCM) layer, and indicated that there was no significant correlation relationship between bbp(λ) and Chla in the upper layer of the DCM. Particles smaller than 3.2 µm that were in the water column above the DCM layer had a large effect on the bbp(λ) in the vertical profile, but particles larger than 3.2 µm and smaller than 10 µm had the largest effect on the bbp(λ) in the water column below the DCM layer. The contribution of non-algal particles (NAPs) to backscattering is up to 50%, which occurs in the water depth of 50 m and not consistent with the distribution of Chla. Phytoplankton and NAPs were modeled as coated spheres and homogeneous spherical particles to simulate the bbp(λ) of the vertical profile by Aden-Kerker method and Mie theory, and the results also indicated that the backscattering caused by particles less than 20 µm were closer to the measured data when they were below and above the DCM layer, respectively. This relationship also reflects the bbp(λ) of particles in the upper water was significantly affected particle size, but bbp(λ) in the lower water was significantly affected by Chla concentration. This effect may have relationship with phytoplankton photoacclimation and the relationship of a phytoplankton biomass maximum with particle size distribution in the water column according to the previous relevant studies. These characteristics also had spatial and seasonal variations due to changes of Chla concentration at the surface and at different depths. There was mostly a linear relationship between Chla and bbp(700) during winter. During other seasons, the relationship between these two variables was better characterized by a power function (or a logarithmic function) in the lower layer of the DCM. The spatial and vertical relationships between the bbp(λ) and Chla and the corresponding variations in the types of particles described in this study provide parameters that can be used for accurate estimation of regional geochemical processes.
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Clorofila , Água , Clorofila A , Oceano Pacífico , Oceanos e Mares , Biomassa , Fitoplâncton/químicaRESUMO
BACKGROUND: In the early stages of the coronavirus disease 2019 (COVID-19) outbreak, the most widely recognised symptoms of the disease were fever, cough, shortness of breath, myalgia, and fatigue. However, in addition to these symptoms, COVID-19 can cause systemic symptoms outside the lungs. Older patients with severe COVID-19 often require admission to the intensive care unit (ICU). Acute rectal ulcer bleeding, characterised by painless, profuse haematochezia, caused by solitary or multiple rectal ulcers, is one of the main causes of severe haematochezia in patients with COVID-19 in the ICU. However, recurrent duodenal ulcer bleeding followed by rectal ulcer bleeding has not previously been reported in older patients during ICU treatment for severe COVID-19. CASES PRESENTATION: Herein, we report the case of an 81-year-old woman admitted to the emergency department due to severe COVID-19 and transferred to the ICU 2 days later for treatment. During treatment in the ICU, the patient developed recurrent duodenal ulcer bleeding and underwent endoscopic electrocoagulation haemostasis and gastroduodenal artery embolisation. However, the night after the final haemostatic operation, due to rectal ulcer bleeding, the patient discharged bloody stools intermittently, which was effectively controlled using endoscopic electrocoagulation, topical medication, blood transfusion, and haemostatic drugs. CONCLUSIONS: To the best of our knowledge, this is the first report of duodenal ulcer bleeding followed by rectal ulcer bleeding in an older patient with severe COVID-19 infection. This report creates awareness for clinicians about the multiple and complex gastrointestinal symptoms that may occur during COVID-19 treatment.
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COVID-19 , Úlcera Duodenal , Feminino , Humanos , Idoso , Idoso de 80 Anos ou mais , Úlcera , Úlcera Duodenal/complicações , Úlcera Duodenal/diagnóstico , Úlcera Duodenal/terapia , Tratamento Farmacológico da COVID-19 , COVID-19/complicações , COVID-19/diagnóstico , COVID-19/terapia , TosseRESUMO
OBJECTIVES: To explore the association of KISS1, LIN28B, vitamin D receptor (VDR), and estrogen receptor α (ERα) gene polymorphisms and the risk of early with fast puberty (EFP) risk, and with hormone levels in EFP cases, in Chinese girls. METHODS: The analysis was based on the data of 141 girls with EFP and 152 girls without EFP. Clinical features were documented, and all SNP genotyping was conducted using SNaPshot method. Statistical analysis was performed to assess the association of the SNPs with EFP risk, and with hormone levels in EFP cases. RESULTS: There was a significant association between rs7759938-C polymorphism in the LIN28B gene and the risk for EFP in the recessive (TT + CT vs. CC) model (p = 0.040). Remarkably, rs5780218-delA polymorphism in the KISS1 gene and rs2234693-C polymorphism in the ERα gene were significantly associated with peak LH (luteinizing hormone) levels (p = 0.008, 0.045) and peak LH/FSH (follicle-stimulating hormone) ratio (p = 0.007, 0.006). Additionally, on 7 of the 8 variant loci the alleles associated with increased levels of both peak LH levels and peak LH/FSH ratio in EFP cases were also associated with increased CPP risk. CONCLUSIONS: Our findings indicate that rs7759938-C polymorphism in the LIN28B gene might have a protective effect on EFP susceptibility. The most striking findings of this study is that, rs5780218-delA polymorphism in the KISS1 gene and rs2234693-C polymorphism in the ERα gene influenced levels of GnRH-stimulated peak LH and LH/FSH ratio, and in general CPP risk genes might also contributes to the abnormality of hormonal levels in EFP.
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Receptor alfa de Estrogênio , Kisspeptinas , Puberdade Precoce , Puberdade , Proteínas de Ligação a RNA , Receptores de Calcitriol , Feminino , Humanos , População do Leste Asiático , Receptor alfa de Estrogênio/genética , Hormônio Foliculoestimulante Humano , Hormônio Liberador de Gonadotropina/genética , Kisspeptinas/genética , Hormônio Luteinizante/metabolismo , Polimorfismo de Nucleotídeo Único , Puberdade/genética , Puberdade Precoce/genética , Receptores de Calcitriol/genética , Proteínas de Ligação a RNA/genéticaRESUMO
BACKGROUND: Pancreatic cancer is a fatal malignancy of the digestive system and the 5-year survival rate remains low. Therefore, new molecular therapeutic targets are required to improve treatments, prognosis, and the survival of patients. N6-methyladenosine (m6A) is the most prevalent reversible methylation in mammalian messenger RNA (mRNA) and has critical roles in the tumorigenesis and metastasis of various malignancies. However, the role of m6A in pancreatic cancer is still unclear. Exploring genetic alterations and functional networks of m6A regulators in pancreatic cancer may provide new strategies for its treatment. METHODS: In this study, we used data from the Cancer Genome Atlas (TCGA) database and other public databases through cBioPortal, LinkedOmics, UALCAN, GEPIA, STRING, and the database for annotation, visualization, and integrated discovery (DAVID) to systematically analyze the molecular alterations and functions of 20 main m6A regulators in pancreatic cancer. RESULTS: We found that m6A regulators had widespread genetic alterations, and that their expression levels were significantly correlated with pancreatic cancer malignancy. Moreover, m6A regulators were associated with the prognosis of pancreatic cancer patients. CONCLUSIONS: m6A regulators play a crucial part in the occurrence and development of pancreatic cancer. Our study will guide further studies of m6A RNA modification in pancreatic cancer and could potentially provide new strategies for pancreatic cancer treatment.
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Regulação Neoplásica da Expressão Gênica , Neoplasias Pancreáticas , Animais , Mineração de Dados , Humanos , Metilação , Neoplasias Pancreáticas/genética , RNA Mensageiro/metabolismoRESUMO
Health problems caused by environmental pollution may affect the process of urbanization in China. Therefore, this study, against the backdrop of promoting new-type urbanization, evaluates the level of China's urbanization comprehensively using the fully arranged polygon graphical index method. It uses a dynamic threshold panel model to study the potential non-linear relationship between environmental pollution (wastewater, sulfur dioxide, and solid wastes) and urbanization under different health costs of residents. Our findings show that environmental pollution has inhibited the improvement of comprehensive urbanization, population urbanization, economic urbanization, and living conditions urbanization, but promoted living environment urbanization, in China. It is worth noting that with the rise in residents' health costs, the inhibiting effect of environmental pollution on comprehensive urbanization, population urbanization, economic urbanization, and living conditions urbanization in China has gradually increased, but on living environment urbanization, it has decreased.
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Poluição Ambiental , Custos de Cuidados de Saúde , Urbanização , China , Humanos , Dióxido de EnxofreRESUMO
Our earlier work showed that Musashi (MSI)-2 promoted the development of pancreatic cancer (PC) by down-regulating Numb, which prevented murine double-minute (MDM)-2-mediated p53 ubiquitin degradation. Thus, we investigate the relationship among MSI2, Numb, MDM2, and p53 in PC in vitro and invivo, an association that has not been reported to our knowledge. MSI2 had no relationship with mutant p53 (mtp53) and wild-type p53 (wtp53) in normal PC cells. However, in response to gemcitabine or cisplatin treatment, MSI2 silencing simultaneously down-regulated MDM2 and up-regulated Numb and wtp53 protein levels. Moreover, these 4 endogenous proteins can be coimmunoprecipitated as a quaternary complex. Numb small interfering RNA (siRNA) reversed the MSI2 silencing-induced p53 increase. During treatment with chemical agents, MSI2 silencing decreased drug resistance and cell motility in vitro and inhibited tumor growth in vivo, all of which were significantly reversed by p53 siRNA. MSI2 was also negatively associated with Numb and positively associated with MDM2 expression in tissue. Overexpression of MSI2, MDM2, and mtp53 and weak expression of Numb were closely associated with aggressive clinicopathologic characteristics and poor prognosis for patients with PC. MSI2 negatively regulates wtp53 protein by up-regulating MDM2 and down-regulating Numb after treatment with chemical agents. MSI2 promotes drug resistance and malignant biology of PC in a p53-dependent manner.-Sheng, W., Dong, M., Chen, C., Wang, Z., Li, Y., Wang, K., Li, Y., Zhou, J. Cooperation of Musashi-2, Numb, MDM2, and P53 in drug resistance and malignant biology of pancreatic cancer.
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Regulação Neoplásica da Expressão Gênica/fisiologia , Proteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Neoplasias Pancreáticas/metabolismo , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteínas de Ligação a RNA/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Resistencia a Medicamentos Antineoplásicos , Feminino , Inativação Gênica , Humanos , Proteínas de Membrana/genética , Camundongos , Camundongos Nus , Neoplasias Experimentais , Proteínas do Tecido Nervoso/genética , Neoplasias Pancreáticas/tratamento farmacológico , Proteínas Proto-Oncogênicas c-mdm2/genética , Interferência de RNA , Proteínas de Ligação a RNA/genética , Proteína Supressora de Tumor p53/genéticaRESUMO
The Fourier interferometric spectrometer (FIS) acquires the interference data information of the spectrum and during the spectrum data processing, a series of spectrum reconstruction will be performed on the interference information to obtain the final spectrum information data. The spectral calibration is the key step to spectrum reconstruction of FIS, which directly determines accuracy and availability of the spectrum results. This paper introduces the basic ideas and calibration accuracy about the spectral calibration for the FIS and puts forward a new spectral calibration method based on calculating the precise value of the total optical path difference (TOPD). The TOPD of FIS is difficult to be precisely measured, but it is the core and key to the spectral calibration. In order to calculate the precise TOPD, this paper proposes the idea how to traverse the TOPD and analyzes the spectrum drift. During the calibration, all the possible values of the TOPD participate in the spectrum reconstruction flow to carry out spectrum recovery and analysis. Ultimately the TOPD with the minimum spectrum drift will be achieved, namely solution value of the TOPD. This method can accurately resolve the TOPD of the FIS and then calibrate the spectrum with high accuracy. In addition, the paper introduces the detailed and complete spectral calibration flow and obtains the center wavelength value of every band and wavenumber resolution. Moreover, the paper designs the main parameters of the typical FIS and generates its simulation interference data. Using the above method to calibrate the simulation data, the analysis and verification of the spectral calibration results proves that the calibration precision of wavenumber resolution achieves 0.000 25 cm⻹ or above.
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This meta-analysis of published cohort studies was conducted to evaluate how closely the promoter methylation of the vimentin gene is correlated with the pathogenesis of colorectal carcinogenesis (CRC). The Web of Science (1945 ~ 2013), Cochrane Library Database (issue 12, 2013), PubMed (1966 ~ 2013), EMBASE (1980 ~ 2013), CINAHL (1982 ~ 2013), and Chinese Biomedical Database (CBM) (1982 ~ 2013) were searched without language restrictions. Meta-analyses were conducted using Stata software (Version 12.0, Stata Corporation, College Station, TX, USA). Odds ratios (ORs) and 95 % confidence intervals (95 %CI) were calculated. Seven clinical cohort studies with a total of 467 CRC subjects met our inclusion criteria. Our meta-analysis results demonstrated that the frequency of vimentin promoter methylation in cancer tissues was significantly higher than in normal and benign tissues (cancer tissues vs. normal tissues: OR = 32.41, 95 %CI = 21.04 ~ 49.93, P < 0.001; cancer tissues vs. benign tissues: OR = 1.60, 95 %CI 1.05 ~ 2.42, P = 0.028). Ethnicity-stratified analysis indicated that the frequency of aberrant vimentin promoter methylation was correlated with the pathogenesis of CRC in both Asians and Caucasians. The findings of our meta-analysis confirm that vimentin methylation may play a crucial role in the pathogenesis of CRC.
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Carcinogênese , Neoplasias Colorretais/genética , Metilação de DNA/genética , Vimentina/genética , Neoplasias Colorretais/patologia , Predisposição Genética para Doença , Humanos , Regiões Promotoras Genéticas , Fatores de RiscoRESUMO
Triclosan (TCS), a commonly used antibacterial agent, is associated with various harmful effects on mammalian neurodevelopment, particularly when exposed prenatally. This study investigated the impact of long-term exposure to TCS on the prefrontal cortex development in adolescent mice. We evaluated the motor ability, motor coordination, and anxiety behavior of mice using open field tests (OFT) and elevated cross maze tests (EPM). An increase in movement distance, number of passes through the central area, and open arm retention time was observed in mice treated with TCS. Hematoxylin eosin staining and Nissl staining also showed significant adverse reactions in the brain tissue of TCS-exposed group. TCS induced microglia activation and increased inflammatory factors expression in the prefrontal cortex. TCS also increased the expression of pyruvate kinase M2 (PKM2), thereby elevating the levels of PKM2 dimer, which entered the nucleus. Treatment with TEPP46 (PKM2 dimer nuclear translocation inhibitor) blocked the expression of inflammatory factors induced by TCS. TCS induced the phosphorylation of nuclear signal transducer and activator of transcription 3 (STAT3) in vivo and in vitro, upregulating the levels of inflammatory cytokines. The results also demonstrated the binding of PKM2 to STAT3, which promoted STAT3 phosphorylation at the Tyr705 site, thereby regulating the expression of inflammatory factors. These findings highlight the role of PKM2-regulated STAT3 phosphorylation in TCS-induced behavioral disorders in adolescents and propose a reliable treatment target for TCS.
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Microglia , Doenças Neuroinflamatórias , Piruvato Quinase , Fator de Transcrição STAT3 , Triclosan , Animais , Triclosan/toxicidade , Camundongos , Microglia/efeitos dos fármacos , Piruvato Quinase/metabolismo , Fator de Transcrição STAT3/metabolismo , Fosforilação , Doenças Neuroinflamatórias/induzido quimicamente , Anti-Infecciosos Locais/toxicidade , MasculinoRESUMO
The clinically used glitazones (rosiglitazone and pioglitazone) for type 2 diabetes mellitus therapy have been linked to serious side effects such as fluid retention, congestive heart failure, weight gain, bone loss, and an increased risk of bladder cancer. The complete activation of PPAR-γ receptors in target tissues is linked to these effects. Many studies have demonstrated that partial PPAR-γ activators (GW0072, PAT5A, GQ16) give equivalent therapeutic benefits to full PPAR-γ agonists without the associated side effects. These breakthroughs cleared the path for the development of partial agonists or selective PPAR-γ modulators (SPPARγMs). This study combined pharmacophore modeling, molecular docking, and an adipogenesis experiment to identify thiazolidine analogs as SPPARMs/partial agonists. A custom library of 220 molecules was created and virtual screened to discover 90 compounds as SPPARγMs/ partial agonists. The chosen eight compounds were synthesized and tested for adipogenesis using 3T3L1 cell lines. These compounds' partial agonistic activity was evaluated in 3T3L1 cell lines by comparing their capacity to stimulate PPAR-γ mediated adipogenesis to that of a full agonist, rosiglitazone. The findings of the adipogenesis experiment demonstrate that all eight compounds examined had a partial potential to stimulate adipogenesis when compared to the full agonist, rosiglitazone. The current investigation identified eight possible PPAR-γ partial agonists or SPPARγMs that may be effective in the treatment of type 2 diabetes mellitus.
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Diabetes Mellitus Tipo 2 , Tiazolidinedionas , Humanos , Rosiglitazona/farmacologia , Rosiglitazona/uso terapêutico , PPAR gama/agonistas , PPAR gama/metabolismo , PPAR gama/uso terapêutico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Hipoglicemiantes/farmacologia , Hipoglicemiantes/uso terapêutico , Simulação de Acoplamento Molecular , Adipogenia , Farmacóforo , Tiazolidinedionas/farmacologia , Tiazolidinedionas/uso terapêuticoRESUMO
MicroRNAs (miRNAs/miRs) are key components of regulatory networks in cancer. Although miR190b is an important tumorrelated miRNA, its role in pancreatic cancer has not been extensively investigated. The aim of the present study was to examine the expression of miR190b in pancreatic cancer cell lines and tissues and evaluate its effects on cancer progression. Reverse transcriptionquantitative PCR (RTqPCR) analysis was used to measure miR190b expression levels in human pancreatic cancer cell lines and tissues, and the association between miR190b expression and clinicopathological characteristics was assessed. An in vitro Transwell invasion assay and an in vivo metastasis formation assay were performed using pancreatic cancer cells. The effect of miR190b on pancreatic cancer cell proliferation was evaluated using a Cell Counting Kit8 assay based on an in vivo xenograft mouse model. The direct targets of miR190b were predicted using bioinformatics tools and were validated through western blotting and luciferase reporter assays. Pancreatic cancer cell lines and tissues were found to express lower levels of miR190b compared with normal cells and adjacent nontumor tissues. Furthermore, high expression of miR190b was found to be positively correlated with low T, N and American Joint Committee on Cancer classifications, and predicted a good prognosis. miR190b was shown to exert suppressive effects on cancer cell proliferation, invasion and metastasis. In addition, it was also found that miR190b directly targeted myocyte enhancer factor 2C (MEF2C) and transcription factor 4 (TCF4) in pancreatic cancer, thus serving as a tumor suppressor and a predictor of good prognosis in pancreatic cancer. The immunohistochemistry and RTqPCR results indicated that the MEF2C and TCF4 expression levels were negatively correlated with the miR190b expression levels. The findings of the present study highlight the value of miR190b as a novel target candidate for pancreatic cancer diagnosis and therapy.
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Proliferação de Células/genética , MicroRNAs/genética , Neoplasias Pancreáticas/genética , Fator de Transcrição 4/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Linhagem Celular Tumoral , Progressão da Doença , Feminino , Humanos , Fatores de Transcrição MEF2/genética , Masculino , Camundongos , Pessoa de Meia-IdadeRESUMO
[This retracts the article DOI: 10.1016/j.omtn.2021.06.005.].
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Central precocious puberty (CPP) is associated with adverse health outcomes in females; however, CPP pathogenesis remains unclear. In this study, we investigated the association of 20 single nucleotide polymorphisms (SNPs) in eight genes with CPP risk and hormone levels. A case-control study on 247 and 243 girls with and without CPP, respectively, was conducted at Kunming Children's Hospital, China, from September 2019 to August 2020. The genotype of the SNPs and their haplotypes were identified. Additionally, the effects of the polymorphisms on hormone levels were investigated. Three variants (rs10159082, rs7538038, and rs5780218) in KISS1 and two variants (rs7895833 and rs3758391) in SIRT1 were related to an increased CPP risk (odds ratio (OR) = 1.524, 1.507, 1.409, 1.348, and 1.737; 95% confidence interval (CI) = 1.176-1.974, 1.152-1.970, 1.089-1.824, 1.023-1.777, and 1.242-2.430, respectively). Rs3740051in SIRT1 and rs1544410 in VDR reduced CPP risk (OR = 0.689, 0.464; 95% CI, 0.511-0.928, 0.232-0.925, respectively). Rs1544410, rs7975232, and rs731236 in VDR were negatively correlated with peak follicle-stimulating hormone (FSH; ß = -2.181; P=0.045), basal FSH (ß = -0.391; P=0.010), and insulin-like growth factor (ß = -50.360; P=0.041) levels, respectively. KISS1, SIRT1, and VDR variants were associated with CPP susceptibility, and VDR SNPs influenced hormonal levels in Chinese females with CPP. In particular, VDR polymorphism rs1544410 was associated with both CPP risk and GnRH-stimulated peak FSH levels. Further functional research and large-scale genetic studies of these loci and genes are required to confirm our findings.
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We aim to explore the intestinal microbial metabolites in preterm infants with noninvasive methods and analyze the effects of initial feeding methods. Preterm infants with gestational weeks lower than 34 were recruited for fecal sample collection every 7 days. Fecal pH, ammonia, bile acid, and secretory IgA (sIgA) were tested. A 1:10 fecal slurry was inoculated into different culture media containing different carbohydrates as the only carbon source: lactose (LAT), fructooligosaccharide (FOS), galactooligosaccharide (GOS), and 2'-fucosyllactose (FL2). After 24 h of anaerobic culture through an in vitro fermentation system, air pressure difference, carbohydrate degradation rate, and short-chain fatty acids (SCFAs) content in fermentation pots were measured. Preterm infants were assigned into two groups: group A, preterm infants fed by human milk, including mother's own milk and donor human milk (DHM); group B, preterm infants fed by preterm formula at first 3 days and fed by human milk (including mother's own milk and DHM) from day 4 to discharge. Group A included 90 samples and group B included 70 samples. Group A had lower fecal pH (p = 0.023), ammonia (p = 0.001), and bile acids (p = 0.025). Group B also had higher fecal sIgA levels, both in OD (p = 0.046) and concentration (p < 0.0001) methods. Carbohydrates degradation rates in group A were higher than group B, especially in LAT medium (p = 0.017) and GOS medium (p = 0.005). Gas production amount had no significant difference in all four media. Several different SCFAs in four kinds of different culture media in group A were higher than in group B, but valeric acid was lower in group A. The initial feeding methods may affect the preterm infants' intestinal microecology and microbial metabolites for at least several weeks.
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Backgroud: Renal fibrosis is the common characteristic of chronic kidney disease. Circular RNA plays an essential role in the occurrence and development of Renal fibrosis, but its regulative mechanism remains elusive. Methods: The animal and cell model of Renal fibrosis was established, and RNA-sequencing and real-time polymerase chain reaction (qRT-PCR) experiments were implemented. Subsequently, experiments for detecting apoptosis and proliferation of cell, were carried out, and the isobaric tags for relative and absolute quantification proteomics analyses were performed accordingly. Results: It was found that a newly discovered Circular RNA (circRNA_0002158), is highly expressed in kidneys or cells with fibrosis, implying that this Circular RNA might be associated with the occurrence and development of Renal fibrosis. Subsequently, the overexpression and knockdown of circRNA_0002158 were conducted in the human kidney epithelial cell line (HK-2) cells, and the results indicated that the circRNA_0002158 could inhibit apoptosis, and promote proliferation of cells. The kidney injury-related factors, including Fibronectin and plasminogen activator inhibitor-1 (PAI-1), were decreased in HK-2 cells with overexpression of circRNA_0002158, while the results were reversed in cells with knockdown of circRNA_0002158. Finally, to explore the regulative mechanism of circRNA_0002158, the iTRAQ proteomics analyses were implemented for the cell samples with OE of circRNA_0002158 and its control, it showed that multiple genes and functional pathways were associated with the occurrence and development of Renal fibrosis. Conclusion: CircRNA_0002158 is associated with regulating Renal fibrosis, and may contribute to ameliorating the progression of Renal fibrosis in the future.
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Pancreatic cancer is the deadliest malignancy of the digestive system and is the seventh most common cause of cancer-related deaths worldwide. The incidence and mortality of pancreatic cancer continue to increase, and its 5-year survival rate remains the lowest among all cancers. N6-methyladenine (m6A) is the most abundant reversible RNA modification in various eukaryotic messenger and long noncoding RNAs and plays crucial roles in the occurrence and development of cancers. However, the role of m6A in pancreatic cancer remains unclear. The present study aimed to explore the role of m6A and its regulators in pancreatic cancer and assess its underlying molecular mechanism associated with pancreatic cancer cell proliferation, invasion, and metastasis. Reduced expression of the m6A demethylase, fat mass and obesity-associated protein (FTO), was responsible for the high levels of m6A RNA modification in pancreatic cancer. Moreover, FTO demethylated the m6A modification of praja ring finger ubiquitin ligase 2 (PJA2), thereby reducing its mRNA decay, suppressing Wnt signaling, and ultimately restraining the proliferation, invasion, and metastasis of pancreatic cancer cells. Altogether, this study describes new, potential molecular therapeutic targets for pancreatic cancer that could pave the way to improve patient outcome.
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Long non-coding RNAs (lncRNAs) play important roles in human diseases. They control gene expression levels and influence various biological processes through multiple mechanisms. Functional abnormalities in lncRNAs are strongly associated with occurrence and development of various diseases. LINC00472, which is located on chromosome 6q13, is involved in several human diseases, particularly cancers of the breast, lung, liver, osteosarcoma, bladder, colorectal, ovarian, pancreatic and stomach. Importantly, LINC00472 can be used as a biomarker for breast cancer cell sensitivity to chemotherapeutic regimens, including doxorubicin. LINC00472 is regulated by microRNAs and several signaling pathways. However, the significance of LINC00472 in human diseases has not been clearly established. In this review, we elucidate on the significance of LINC00472 in various human diseases, indicating that LINC00472 may be a diagnostic, prognostic as well as therapeutic target for these diseases.
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A small subgroup of embryonic stem cells (ESCs) exhibit molecular features similar to those of two-cell embryos (2C). However, it remains elusive whether 2C-like cells and 2C embryos share similar epigenetic features. Here, we map the genome-wide profiles of histone H3K4me3 and H3K27me3 in 2C-like cells. We found that the majority of genes in 2C-like cells inherit their histone status from ESCs. Among the genes showing a switch in their histone methylation status during 2C-like transitions, only a small number acquire 2C-embryo epigenetic signatures. In contrast, broad H3K4me3 domains display extensive loss in 2C-like cells. Most of the differentially expressed genes display decreased H3K4me3 and H3K27me3 levels in 2C-like cells, whereas de novo H3K4me3 deposition is closely linked with the expression levels of upregulated 2C-specific genes. Taken together, our study reveals the unique epigenetic profiles of 2C-like cells, facilitating the further exploration of totipotency in the future.
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Embrião de Mamíferos/fisiologia , Células-Tronco Embrionárias/fisiologia , Epigênese Genética , Regulação da Expressão Gênica no Desenvolvimento , Histonas/genética , Histonas/metabolismo , Animais , Células Cultivadas , Sequenciamento de Cromatina por Imunoprecipitação , Feminino , Estudo de Associação Genômica Ampla , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Metilação , Camundongos , Regiões Promotoras Genéticas , Organismos Livres de Patógenos Específicos , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Air pollution is known to trigger and exacerbate many respiratory diseases. The interaction between respiratory microbiome and host plays a significant role in maintaining airway immune homeostasis and health. Emerging evidence has revealed the associations of disturbances in the airway microbiome with air pollution and respiratory disease. However, respiratory microbiome has been an undervalued player in progressions of respiratory disease caused by air pollution. In this review, we summarize the current research advances with respect to the effects of air pollution on respiratory microbiome, then discuss the underlying mechanisms of air pollution induction of dysbiosis in respiratory microbiota and its links to respiratory diseases. This work may be helpful to deepening understanding the relationships between exposure, microbiome and airway disease and discovering new preventive and therapeutic strategies for air pollution-mediated respiratory disease.
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Poluentes Atmosféricos/toxicidade , Poluição do Ar/efeitos adversos , Microbiota/efeitos dos fármacos , Material Particulado/toxicidade , Sistema Respiratório/efeitos dos fármacos , Doenças Respiratórias/induzido quimicamente , Humanos , Exposição por Inalação/efeitos adversos , Sistema Respiratório/microbiologia , Doenças Respiratórias/microbiologiaRESUMO
China's rapid economic development has resulted in increasingly serious environmental pollution that is negatively affecting the health of Chinese citizens. Notably, the unfair distribution of resources may cause the uneven distribution of the environmental burden. In this study, panel data of 30 Chinese provinces and 32 industries for the period 2004 to 2017 is used to investigate how the environmental burden is distributed across different regions and industries. To manage potential endogeneity and allow for dynamics, the generalized method of moments and panel vector autoregression models are employed. The estimation results indicate that, on the whole, urban residents endure the most serious environmental pollution. Notably, a big gap is observed between urban and rural residents' share of environmental pressure, and a similar gap is also observed between developed and underdeveloped areas in China. Moreover, the government is responsible for less environmental pressure than companies for urban residents and rural residents. The subindustry regression results indicate that the "polluting department" bears the most environmental pressure, and the "green department" also bore some negative environmental pressure.